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INDONESIA
JURNAL PEMULIAAN TANAMAN HUTAN
Published by Badan Penelitian dan Penegembangan Kehutanan
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 249 Documents
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ISOLASI, IDENTIFIKASI DAN KARAKTERISASI PENYEBAB PENYAKIT KARAT DAUN PADA SEMAI PINUS DI PERUM PERHUTANI BKPH PURWOREJO, KPH KEDU SELATAN Nur Hidayati; Siti Husna Nurrohmah; Fithry Ardhany
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 1 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.1.21-32

Abstract

Pinus merkusii, a multiuse tree, is cultivated at Indonesian Plantation. Pinus plantation have been threatened by rust/spot leaf disease. The pathogen has infected pinus seedlings at Perum Perhutani, BKPH Purworejo, KPH Kedu Selatan and cause high mortality. The aims of this research to identify pathogen causing rust/spot leaf thus information how to control can be observed. Pathogens were isolated from infected leaves, and grown on the potato dextrose agar (PDA) and observed macroscopis and microscopis and identified. The pathogens also were inoculated to healthy seedlings (Postulat Koch Test). The result shows that pathogen causing rust/spot leaf on seedlings pine is Pestalotia sp with characteristic white colony, hypha has aservuli with conidia that have 25 septums and at the edge, some structure looks like feather whipped 3-5. Potulat Koch test indicated that inoculated healthy seedling shows similar symptom with infected seedlings. Rust/spot leaf has similar sympto and sign. Spot or rust appear on leaves started from edge and spread to base. At first, spots were formed in small size and separate each other but they developed and became larger and wider and fused.
SIFAT KAYU JATI UNGGUL NUSANTARA (Tectona grandis L.f.) PADA TIGA KELAS DIAMETER POHON Gudiwidayanto Sapto Putro; Sri Nugroho Marsoem; Joko Sulistyo; Suryo Hadiwinoto
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 1 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.1.9-19

Abstract

Increasing the growth rate of teak trees is carried out to shorten the tree harvest rotation and reduce the deficit of teak timber supply, while maintaining the superiority of wood properties. The study was conducted to study the nature of five-year-old JUN Teak wood in three classes of tree diameter and radial position of the trunk. This study employed a completely randomized design arranged in factorial combinations of the treatments of radial positions and diameter classes consisting of large (18-22 cm), medium (14-18 cm) and small (10-14 cm) stem diameters, and the radial position of wood on the trunk based on the tree's ring. Three samples of trees were felled for each stem diameter class so that totally nine trees were used in this study. The testing of the physical properties of wood and the measurement of fiber dimensions were conducted using British Standards 373 and the IAWA method, respectively. The results showed that the diameter of the tree and the radial position of the wood had a significant effect on all parameters observed (fiber dimension and physical properties of wood), except the fiber diameter. The tree growth rate had a negative correlation with fiber length (-0.67), fiber wall thickness (-0.65), air-dry moisture content (-0.91) and air dry specific gravity (-0.86), and had a positive correlation with fiber diameter (0.61) and green water content (0.78). According to the pattern of wood properties in the radial direction, JUN wood harvested at the age of five was categorized as juvenile wood. This wood is acceptable as furniture and lightweight construction material.
PERTUMBUHAN STEK BATANG GMELINA ARBOREA HASIL KOLEKSI DARI LIMA POPULASI SEBARAN DI INDONESIA Hamdan Adma Adinugraha; Dedi Setiadi; Arnoldus Naibini; Nyuwito Nyuwito
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.83-91

Abstract

This study was conducted to determine the effect of plant material origin and size of cuttings on the growth ability of Gmelina. arborea stem cuttings in the nursery. The study was arranged in a randomized factorial pattern design, consisting of two factors, namely the length of the cuttings (L1 = 20 cm and L2 = 30 cm) and the cuttings size factor (small diameter class/D1 = <1.25 cm, medium/D2 = 1.25 – 2.50 cm and large/D3 => 2.50 cm). Each treatment used 5 stem cuttings and repeated in 3 times, which are grouped based on their origin population namely Bantul/P1, Bogor/P2, Lampung/P3, Bondowoso/P4 and Lombok/P5. The observationsshowed the survival percentage was 83.12% and the rooting percentage was 78.23% which was influenced by the length and diameter of the stem cuttings. The growth of shoots (number, length and diameter) and the number of shoot nodes and leaf were affected by the interaction of origin of the population and the length and diameter of the stem cuttings. Applying stem cutting method is very potential to produce planting stock of Gmelina arbore. Stem cuttings which has a biger size showed better grow of stem cuttings.
DETEKSI DAN IDENTIFIKASI Austropuccinia psidii PADA MYRTACEAE DI YOGYAKARTA INDONESIA Istiana Prihatini; I. L. G. Nutjahjaningsih; Farah Aulya Faradilla; Suranto Suranto
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.101-110

Abstract

Austropuccinia psidii is a pathogenic fungus that causes rust in the Myrtaceae plant. The extensive plantation of the host of this fungus has increased the attack of fungal pathogen, therefore, will increase the threat to the presence of Myrtaceae species around the globe including in Indonesia. This present study was aiming to detect and identify the presence of this pathogen by morphological and molecular observation. Morphological observation revealed the presence of A. psidii urediniospores on salam (Syzygium polyanthum) and kayu putih (Melaleuca cajuputi) leaves collected from the arboretum of the Indonesian Center for Forest Biotechnology and Tree Improvement (CFBTI), and the presence of teliospores on young Syzygium leaves. PCR amplification using specific primers of Ppsi1 / Ppsi6 succeeded in detecting the presence of A. psidii fungi Melaleuca and Syzygium showed by DNA amplicon length around 500bp. Efforts to obtain ITS DNA sequences to compare the molecular characteristics of fungi from two different hosts have been carried out, however, the sequencing electropherogram was unreadable, so the comparison can not be performed. This study reported that A. psidii is currently present in Myrtaceae species in Yogyakarta, therefore precaution efforts should be conducted to avoid economic and ecological impact from this pathogen.
INVENTARISASI, KARAKTERISASI MORFOLOGI, DAN UJI VIABILITAS BENIH PACAR TERE (Impatiens platypetala Lindl.) HASIL EKSPLORASI DI KAWASAN TAMAN NASIONAL BROMO TENGGER SEMERU Elga Renjana; Apriyono Rahadiantoro; Linda Wige Ningrum; Dewi Ayu Lestari; Elok Rifqi Firdiana; Trimanto Trimanto; Shofiyatul Mas&#039;udah; Lia Hapsari
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 1 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.1.25-36

Abstract

Bromo Tengger Semeru National Park (BTSNP) is one of natural conservation areas in East Java with around 1,025 plant species. Impatiens platypetala, locally known as pacar tere, is a plant species mostly be found in mountainous areas of Java and with high potential as medicinal and ornamental plant. However, research on this species is still limited. This study was conducted to inventory, describe the morphological characteristics and test the seed viability of pacar tere in four resorts of BTSNP, namely RPTN Gunung Penanjakan, Coban Trisula, Senduro, and Ranu Darungan. Survey method was used to inventory the plant populations.Direct field observation was used to characterize the plant morphology. Seeds viability test was conducted through germination on straw paper media. The results showed that pacar tere populations were found in those four BTSNP resorts, on shade and humid habitats, also in open areas with wet soil conditions. The plant is characterized as an erect parennial herb, spursed pink flower, capsula fruit which dehiscent when ripe, with flattened cone-shaped and smooth hairy seeds. Seed germination test showed that the seeds began to germinate on day 5 after sowing (around 20 sprouts/day), epigeal type, with percentage of germination and viability was 56% and 90% respectively. Results of this study are expected to provide basic information to support the conservation efforts of I. platypetalaboth in-situ and ex-situ (seed and living plant), also its propagation efforts for further bioprospecting researches.
OPTIMASI DETEKSI GEN PADA Stelechocarpus burahol (Bl.) Hook.f. & Th. MENGGUNAKAN DIRECT KIR PCR Tri Suwarni Wahyudiningsih; Dian Sartika
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.93-99

Abstract

Purification of DNA molecules from a large number of samples is laborious, costly, time-consuming, and a high risk of contamination. S. burahol leaves contain phenolic compounds, flavonoids, and terpenoids that can interfere with DNA isolation. Direct PCR kits can detect genes without DNA extraction. The objective of study was to determine the method of gene detection of Stelechocarpus burahol using the direct PCR kit. S. burahol leaf samples came from the Bogor Botanical Gardens (two trees), Garut, Purwodadi Botanical Gardens, Kyai Langgeng Gardens, Yogyakarta Palace, Turi Sleman, Wanagama, Karanganyar, and South Kalimantan. Each leaf sample of 0.1 mg was dissolved into 1.25% w / v SDS (Sodium Dodecyl sulfate) 50 ul solution, incubated at 95o C for 5 minutes, and vortexed for 2 seconds. Primers used for the trials were ITS 1F primers and 4R primers. Pre-denaturation of 95oC for 7 minutes, denaturation of 95oC for 1 minute, annealing 55oC for 1 minute, extension at 72oC for 1 minute, extension at 72oC for 1 minute, and 40 cyclus. PCR product samples of 40 - 50 μl that showed positive results were detected by electrophoresis. The results of S. burahol DNA amplification measuring ± 750 bp from ten samples of S. burahol. Direct PCR kits can be used for S. burahol gene detection, time and energy efficient, only requires a small amount of tissue, and reduces contamination due to DNA extraction. Direct PCR kits can be an effective method that can be utilized to detect target genes for large populations
PENGEMBANGAN METODE PENANDA ISOZYME PADA TREMBESI Titis Hutama Syah; Arbain Arbain
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.137-145

Abstract

Fifty samples of raintree from Sangatta, the capital city of Kutai Timur Regency, East Kalimantan were analyzed using isozyme markers to determine the characteristics of the banding pattern. The use of isozymes was intended as a biochemical marker for genetic diversity analysis. This study aimed to determine the enzyme system that could be used to determine genetic diversity of raintree. The enzyme systems used were diaphorase, esterase, and peroxidase. Polymorphism assessments were carried out on the parameters of expected heterozygosity (H), polymorphism information content (PIC), effective multiplex ratio (E), marker index (MI), discriminanting power (D), and Resolving (R). Among the three enzyme systems used, diaphorase showed consistent performance against each of the parameters assessed, with a value of H =0.475; PIC = 0.362; E =6.1; MI = 2,2; D = 0.63: and R = 4.44. However, esterase had the highest multiplex effective ratio (E = 6.16). Therefore, diaphorase is the best isozyme marker that can be used to analyze the genetic diversity of raintree.
PERTUMBUHAN BIBIT MALAPARI DARI PROVENAN UJUNG KULON PADA UMUR 5 BULAN DI PERSEMAIAN Tri Maria Hasnah; Eritrina Windyarini; Budi Leksono; Hamdan Adma Adinugraha; Lukman Hakim
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 1 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.1.37-49

Abstract

Malapari (Pongamia pinnata) is one of tree species belonging to Family of Leguminosae. Malapari seed oil were known as potential source for biofuel. The previous study showed that Provenance from Taman Nasional Ujung Kulon Banten had highest oil content among provenances in Java. Seed exploration was carried out to determine variations among families on oil content and growth performance. This study was conducted to determine the variation among families on growth performance at nursery level. The seedlings were used as planting stocks for Progeny Test establishment. This study was arranged in randomized completely block design with 50 families, 10 seedlings per plot and repeated in 4 blocks resulting the total number of observation units were 2000 seedlings. Seedling survival rate, growth performance (height, diameter, leave number), and sturdiness ratio was measured monthly up to 5 months after sowing. Analyses of variance was used to find out differences among families. Correlation among characters/parameters was analyzed by Pearson Correlation Analyses. The results showed that variations among families were found on seedling growth performance. The seedling survival rate at the age of 5 months was 84.60% (26,70-100%) with an average growth of 47.10 cm (31,2-59,7 cm) in height, 5.49 mm (4,7-6,5 mm) in diameter, 8.56 for seedlings sturdiness and 15.4 (10,9-18,8) for leave number.
INDUKSI KALUS EMBRIOGENIK DAN EMBRIO SOMATIK DARI EKSPLAN DAUN KULIM (Scorodacarpus borneensis Becc.) Yelnititis Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.73-81

Abstract

Kulim is one of woody plant that have multifunction as wood source and for spice and medicinal. Generative propagation of this plant have trouble because seed use limited. The use of leaf segment through somatic embryogenesis to solve the problem. The objective of this study is to obtain the best treatment to embryogenic callus induction. The modification of basal medium of Murashige and Skoog was used as growth medium. The experiment was conducted in three stages are callus induction, embryogenic callus and somatic embryo induction. The treatment of 2,4-D (3,0 – 12 mg/l) used for callus induction. For embriogenic callus induction used 2,4-D (3,0 – 12,0 mg/l) combined with NAA 0,5 mg/l. The treatment of thidiazuron (0,1 – 0,7 mg/l) used for somatic embryo induction. The result showed that the treatment of 2,4-D 6,0 mg/l is the best for callus induction with compact of texture, green, dry and non embryogenic. The treatment of combination 2,4-D 12.0 mg/l with NAA 0.5 mg/l is the best for friable callus induction. The treatment of 2,4-D 6.0 mg/l combined with NAA 0,5 mg/l is the best for embryogenic callus induction with very friable of texture, easy to separate, dry, smooth and glossy. Thidiazuron of 0,1 mg/l treatment is the best for somatic embryos induction with the average number of 7,8 somatic embryos.
PERTUMBUHAN AWAL KAYU MERAH (Pterocarpus indicus Willd) PADA PLOT KONSERVASI EX-SITU DI GUNUNGKIDUL UMUR 2 TAHUN Yuliah Yuliah; Ari Fiani; Tri Pamungkas
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.129-135

Abstract

Ex-situ conservation plot of kayu merah (Pterocarpus indicus Willd) has been established in Gunungkidul in2016. The purpose of this study was to observe the early growth of kayu merah in the plot at 2 years old. The plot was laid-out in randomized complete block design consisting of 68 families, 4 tree-plot and 5 blocks with spacing of 3 m × 3 m. Genetic materials of kayu merah were collected from 3 populations namely Timor, Flores and Seram. Measurement was conducted in survival rate, tree diameter, tree height and number of branches. The result showed that the average survival rate of family was high (≥80%). Based on the populations, the  survival rate of Timor, Flores and Seram were around 95%, 96% and 96.5% respectively. The average height, diameter and number of branches were 275 cm, 2.79 cm and 1.66, respectively. There were significant differences for height, diameter and number of branches among the tested families. In general, family ranking varied among the measured traits. Correlation between the traits varied among the populations. The Seram population showed the top family ranking for all the traits. It indicated that characterization in ex-situ conservation plot using genetic materials collected from the three populations was important for further conservation program of kayu merah.

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