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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 9 No 1 (2021)" : 15 Documents clear
Isolation and molecular identification of fungi causing stem rot disease in Bali's local legumes Ni Made Susun Parwanayoni; Dewa Ngurah Suprapta; Nyoman Darsini; Sang Ketut Sudirga
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.20426

Abstract

Efforts to improve food security in Indonesia, particularly Bali, need to be supported by improvements in cultivation techniques, including the management of pests and diseases. Meanwhile, legume crops are often attacked by stem rot diseases which potentially decrease production and leads to economic losses. This disease is generally caused by the soil-borne pathogenic fungus Sclerotium rolfsii or Athelia rolfsii. The macroscopic and microscopic morphologies of these two species are the same and difficult to distinguish, hence, molecular identification is needed to differentiate between the species. Therefore, this study aims to isolate and molecularly identify the fungi causing stem rot disease in local legume plants in Bali. The methods used include isolation of pathogenic fungi from legumes showing symptoms of stem rot disease, pathogenicity test, identification of isolates with the highest virulent levels, DNA extraction, DNA amplification by PCR and electrophoresis, ITS region sequencing and computer analysis sequences. The isolation procedure identified six fungal isolates coded SKT, SKB1, SKB2 SKB3, SKL and SKN isolates. SKT isolates had the highest virulence rate as indicated by the pathogenicity test of peanut plants. Furthermore, molecular identification results show that SKT isolate is Athelia rolfsii, a similar clade with the fungi sequences in GenBank with 100% bootstrap support.
Profile of microbial community of organic and conventional rice field using metagenomic analysis Arum Asri Trisnastuti; Edi Purwanto; Ari Susilowati
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.14228

Abstract

Organic farming can increase the diversity of soil bacterial. This research aimed to compare the profile of microbial community of organic and conventional rice fields in early (0 Day After Planting/DAP), mid (15 DAP), and late (45 DAP) fertilizer application period. The total DNA genome from the soil sample was extracted then analyzed metagenomically using Next Generation Sequencing (NGS). There was nine genus of bacteria found in high relative abundance, 95.28%, while 4.72% included in Domain of Archaea (genus Methanosaeta). Phylum of Firmicutes (genus Clostridium has 24.50% relative abundance, Bacillus 11.90%, Lactobacillus 9.69%); Proteobacteria (genus Defluviicoccus 12.10%, Buchnera 18.46%, Rosenbergiella 2.46%); and Actinobacteria (genus Nocardioides 12.21%, and Streptomyces 3.96%). Meanwhile, the average plant height of organic rice fields was shorter than conventional rice fields got directly measured coincided with soil sampled. Based on alpha and beta diversity analysis, the highest community diversity and abundance were found in organic rice field soil samples taken at 45 DAP, i.e., at the end of the fertilizer application period. However, in both organic and conventional rice field soils, there was almost no significant difference in the bacterial community, so it impacts that organic and conventional systems do not make a real difference in the total N, P available, CEC, and pH values. It makes a significant difference in organic C and organic matters.
Biological activity, molecular docking, and ADME predictions of amphibine analogues of Ziziphus spina-christi towards SARS-CoV-2 Mpro Taufik Muhammad Fakih; Dwi Syah Fitra Ramadhan; Fitrianti Darusman
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21335

Abstract

The main protease of the SARS-CoV-2 virus, SARS-CoV-2 Mpro, can be discovered as a promising target to treat the COVID-19 pandemic. The peptide-based inhibitors may present better options than small molecules to inhibit SARS-CoV-2 Mpro. Ziziphus spina-christi species reported have a peptide-based of alkaloids group, i.e., amphibine whose analogues can be identified the potential as an inhibitor of SARS-CoV-2 Mpro. The compound structure was drawn and optimized using semi-empirical AM-1 method using Quantum ESPRESSO v.6.6, while the biological activity using PASS. Prediction server and molecular docking simulation using MGLTools 1.5.6 with AutoDock 4.2 were performed. Afterward, the ADME profiles were predicted using the SWISS-ADME server. PASS server was predicting amphibine B-F and H showed potency both as antiviral and as a protease inhibitor. The molecular docking simulation of amphibine analogues showed lower binding energy than the native ligand. The binding energy of the native ligand was −7.69 Kcal/mol compared to the lowest binding energy of amphibine analogues was −10.10 Kcal/mol (amphibine-F). The ADME prediction showed that amphibine-F has the best bioavailability as an oral drug, amphibine-B, C, and D have good bioavailability, and amphibian-E and H have poor bioavailability. Concluded, amphibine B-F and H of amphibine analogues showed potency as COVID-19 treatment targeting SARS-CoV-2 Mpro.
Typification and taxonomic notes of Smilacaceae species in Java Lulut Dwi Sulistyaningsih; Abinawanto Abinawanto; Marlina Ardiyani; Andi Salamah; Agus Haryadi
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17218

Abstract

Smilacaceae is known as a taxa with wide phenotypic variation and their taxonomical complexities remain unsolved. The three species of Smilacaceae housed in Java, are given nomenclature history. Since all the potential sources of original material have been investigated, but nothing has been identified, three neotypifications were designated here. Herbarium specimen of Koorders 34990β in Herbarium Bogoriense (BO) was chosen as the neotype of Smilax klotzschii. The de Groot & Wehlburg RD52 herbarium specimen in BO was chosen as the neotype of S. nageliana and Blume 463 herbarium specimen in L was chosen as the neotype of S. odoratissima.
Eco-pesantren development: The challenges and opportunities of ecological conservation Kristiyanto Kristiyanto; Hadi Sukadi Alikodra
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21084

Abstract

Implementing green, eco-friendly, and sustainable development is difficult, hence highly dependent on attainable economic profit, and tends to be exploited massively and destructively to attain the desired benefits. It leads to environmental degradation and, consequently, the diminishing of several natural resources. Therefore, an alternative approach involving effective and Islam-based conservation as well as land restoration is required. In this study, in-depth individual interviews on pesantren Al-Zaytun were conducted to collect the qualitative descriptive data, including biotic aspects, such as plant diversity and wastewater treatment in water management and conservation. The results showed the pesantren Al-Zaytun utilized Tectona grandis and Eichhornia crassipes to carry out land and water management as a sustainable approach to conservation as well as food, water, and energy security development. Therefore, it can become a role model for eco-pesantren development in the modern era, as well as the utilization and management of natural resources for ecological development.
Sex identification in Nepenthes adrianii from Baturraden Botanical Garden: Genetic analysis using RAPD Markers Murni Dwiati; Zeihan Aziiza; Agus Hery Susanto
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.15750

Abstract

Nepenthes adrianii is one of pitcher plant species that grows endemically in Mount Slamet, Central Java. At present, it is one of pitcher plant collections of Baturraden Botanical Garden. Since N. adrianii is dioecious and both sexes are difficult to distinguish morphologically, early sex determination supporting its conservation at Baturraden Botanical Garden is needed. One approach can be performed with the use of RAPD molecular markers. Hence, this study aims to know whether differences in RAPD pattern between male and female N. adrianii exist or not and also to find out what the differences are. Genomic DNAs were extracted from leaves of 4 males, 2 females, and 2 sexually unidentified individuals. The extracted DNAs were then used to analyze DNA variation between male and female N. adrianii employing RAPD technique. As many as five oligonucleotide primers (OPA-15, OPK-16, OPP-15, OPP-08, and OPO-08) were used to amplify N. adrianii DNA. The results showed that one primer, i.e. OPK-16 (5’-GAGCGTCGAA-3’), produces a specific band of approximately 290 bp which is only found in female plants. It is assumed that this band is related to gene(s) controlling sex determination in N. adrianii. The RAPD marker can be used for the sex determination of young N. adrianii seedlings.
Stability of watermelon phenotype characters (Citrullus lanatus (Thunb.) Matsum. & Nakai) from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’ Deris Trian Rahmandhias; Wiko Arif Wibowo; Aprilia Sufi Subiastuti; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17719

Abstract

Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is a horticultural plant that belongs to the Cucurbitaceae family with high public demand, however, local markets sometimes have limited supply. The existence of watermelon varieties that are not pest-resistant causes its production to be erratic. Therefore, plant breeding efforts are required to produce superior varieties through the stability test of plant characters. For watermelon to be certified as a new variety, it needs to possess a stable and adaptive character to various conditions. The F1 watermelon from crossing ♀ ‘Putri Delima’ with ♂ ‘Maduri’ produces inole-shaped fruit, red flesh, and a sweet taste. Therefore, this study aims to determine the stability of the phenotypic character of watermelon F2 from crossing ♀ ‘Maduri’ with cultivar ♂ ‘Putri Delima’ and was conducted in Jamusan, Bokoharjo, Prambanan, Sleman, D.I.Yogyakarta fields from August to December. There are five samples of ripe watermelons that were selected randomly while their phenotypic characters were observed qualitatively and quantitatively. Each F2 watermelon character was compared to F1, and the quantitative analysis was conducted using one-factor ANOVA with a confidence level of 5%. The results of quantitative character analysis between F2 and F1 showed a P (P-value)> 0.05. Meanwhile, the results of qualitative observations of F2 watermelon showed different flesh and skin color, while the harvest time from F1 was caused by the segregation of heterozygous crosses. Therefore, it is necessary to select superior phenotypic characters as desired for the next breeding.
Increasing the potential of Cajuput leaf waste as cattle feed through fermentation pretreatment Ana Widiana; Ukit Ukit; Prayoga Kusumah; Ayu Wiharyati; Hana Hanifah
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21215

Abstract

Waste of Cajuput leaves (Melaleuca cajuputi Powell) has the potential to be used as an alternative cattle feed because it has a high nutritional content. The nutritional content of Cajuput leaf waste needs to be improved before being used as an alternative feed. The fermentation process can increase nutrient content in Cajuput leaf waste and increase the organic matter digestibility (OMD), dry matter digestibility (DMD), ammonia (NH3) and volatile fatty acid (VFA). This study aimed to determine the improvement of Cajuput leaf waste nutrition with the help of yeast as well as the effect of fermented Cajuput leaf waste along with a mixture of concentrates and field grass on digestibility, NH3 and VFA which were carried out by in vitro test. The results showed the nutritional content of Cajuput leaf waste fermented by yeast of Aspergillus chevalieri had the best value of protein 16.03%, fiber 16.92% and fat 5.93%. The treatment R4 (50% Cajuput leaf waste + 25% concentrate + 25% field grass) had the best digestibility (DMD= 46.12%; OMD=32.08% ), NH3 (8.37 mM) and VFA (168.5 mM) production.
Morphological and molecular characters of Cherax quadricarinatus (von Martens, 1868) from Sermo Reservoir and Tambakboyo Retention Basin, Daerah Istimewa Yogyakarta, Indonesia Rury Eprilurahman; Aplina Krismutia Simarmata; Lukman Hakim; Trijoko Trijoko
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.16612

Abstract

The Australian red claw freshwater crayfish, Cherax quadricarinatus is one of the most widely distributed and cultivated freshwater crayfish due to its high tolerance towards various environmental conditions. Native to North Australia and South Papua New Guinea, this crayfish was found in Tambakboyo Retention Basin in 2016 and Sermo Reservoir in 2019. This research was aimed to identify the morphological and molecular characters of  C. quadricarinatus collected from Sermo Reservoir and Tambakboyo Retention Basin, Yogyakarta. The genetic information of the samples was compared to Australian red claw freshwater crayfish currently available. The methodology used for this research are morphological, morphometrical, meristic identification, and molecular identification using the PCR method. The primer used to be 1471 primers as the forward primer and 1472 primers as the reverse primer. In conclusion, all six specimens obtained were identified to be C. quadricarinatus. Morphological analysis using UPGMA showed that all specimens were formed one big cluster and has the highest similarity index (1.00). Molecular analysis using BLAST showed that specimen from Sermo Reservoir was 98.96% identical to C. quadricarinatus and specimen from Tambakboyo Retention Basin was 100% identical to C. quadricarinatus. Thus concluding that based on their morphological and molecular character, all samples of this study were C.  quadricarinatus. This finding also contributes to the distribution information of C. quadricarinatus in Daerah Istimewa Yogyakarta
Viability, productivity, and anatomical response of groundnuts (Arachis hypogaea L.) to biofertilizer-sludge biogas applications Nur Hidayah Pangestuti; Dwi Umi Siswanti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.18037

Abstract

Groundnuts (Arachis hypogaea L.) is one of the essential food commodities in Indonesia. The use of biofertilizer has been applied to various types of crops. Meanwhile, the effect of using biofertilizer-sludge biogas on groundnuts is yet unknown. This study aims to analyze the seed viability and vigour, yield productivity, the anatomical response of groundnuts, and optimum concentration that could increase the values of each parameter. Treatments given include applying biofertilizer-sludge with 15 levels of treatment concentration compared to groundnuts without biofertilizer-sludge application as a control. The land was divided into 16 beds for each treatment consisting of control, biofertilizer from 10, 15, 30 L/ha, sludge from 12, 24, to 36 ml, and variations dosage of biofertilizer and sludge combined. The parameters observed for viability and vigour include the percentage of seed germination (GP), seed vigor index (SVI) for yield, the value of harvest index (HI), dry weight of the harvest, and root-shoot ratio (R/S). Anatomical responses were observed with stem diameter, stem’s metaxylem diameter, root diameter, root’s metaxylem diameter, and seed diameter. The biofertilizer-sludge results significantly affected HI, R/S values, stem diameter, root’s metaxylem diameter, and seed diameter. This research concluded that the application of biofertilizer-sludge did not significantly affect the seed viability and vigour and the dry weight of the harvest. The application of biofertilizer-sludge in various doses of concentration resulted in a decrease in the stem metaxylem diameter and root diameter compared to the control. A total of 10 L/ha biofertilizer + 24 ml sludge was an optimum concentration to increasing HI and R/S values. For the increasing stem, root metaxylem, and seed diameter, biofertilizer 30 L/ha + sludge 12 ml, sludge 24 ml, and biofertilizer 15 L/ha + sludge 12 ml were the optimum concentrations, respectively.

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