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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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INDONESIA
Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 332 Documents
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Isolation and Molecular Characterization of Gelatinase-Producing Bacteria from Mangrove Sediment Asep Awaludin Prihanto; Hidayatun Muyasyaroh; Abdul Aziz Jaziri; Nada Itorul Umam
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.10826

Abstract

Protease is an important enzyme widely produced by microorganisms applied in food, health, and industry. Mangrove ecosystem, a rich microorganism habitat, accounted as a new resource for isolating the proteolytic bacteria. The purpose of this study was to identify protease-producing bacteria from mangrove ecosystems in the Tuban area, Indonesia. Three isolates that produced the gelatinase was successfully isolated from mangrove sediments. Bacterial isolates were then tested for extracellular gelatinase. The results showed that isolate T1 had high gelatinase activity. Two isolates (isolates T2 and T3) produced moderately gelatinase enzymes. Molecular identification revealed that isolate T1 is Enterobacter hormaechei.
Performance Comparison of Data Sampling Techniques to Handle Imbalanced Class on Prediction of Compound-Protein Interaction Akhmad Rezki Purnajaya; Wisnu Ananta Kusuma; Medria Kusuma Dewi Hardhienata
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.12002

Abstract

The prediction of Compound-Protein Interactions (CPI) is an essential step in the drug-target analysis for developing new drugs as well as for drug repositioning. One challenging issue in this field is that commonly there are more numbers of non-interacting compound-protein pairs than interacting pairs. This problem causes bias, which may degrade the prediction of CPI. Besides, currently, there is not much research on CPI prediction that compares data sampling techniques to handle the class imbalance problem. To address this issue, we compare four data sampling techniques, namely Random Under-sampling (RUS), Combination of Over-Under-sampling (COUS), Synthetic Minority Over-sampling Technique (SMOTE), and Tomek Link (T-Link). The benchmark CPI data: Nuclear Receptor and G-Protein Coupled Receptor (GPCR) are used to test these techniques. Area Under Curve (AUC) applied to evaluate the CPI prediction performance of each technique. Results show that the AUC values for RUS, COUS, SMOTE, and T-Link are 0.75, 0.77, 0.85 and 0.79 respectively on Nuclear Receptor data and 0.70, 0.85, 0.91 and 0.72 respectively on GPCR data. These results indicate that SMOTE has the highest AUC values. Furthermore, we found that the SMOTE technique is more capable of handling class imbalance problems on CPI prediction compared to the remaining three other techniques.
Diversity of aquatic and terrestrial molluscs from Simeulue Island, with notes on their distribution and some new records Nova Mujiono; Ristiyanti Marsetiyowati Marwoto; Heryanto Heryanto
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.13212

Abstract

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Voluntary Diving Exercise Improves Hippocampus-dependent Learning in Rats Rahadian Yudo Hartantyo; Helen Eko Putro; Epa Yohana Toga Torop; Laksmindra Fitria; Mulyati Mulyati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11168

Abstract

Several physiological alterations that arise in response to hypoxemia conditions when diving are intended to protect oxygen-sensitive organs from the hypoxic condition. Hippocampus, known for its central function in memory formation, is sensitive to hypoxic conditions. This research aimed to study the effect of voluntary diving exercise (VDE) on the hippocampus-dependent learning performance of rats. Rats were divided into control and diving groups, with swim and voluntary diving exercises every day for 60 days, respectively. Observation of memory consolidation was carried out using Morris Water Maze (MWM) and Novel Object Recognition (NOR) tests. In the MWM test, the escape latencies in the diving group were shorter than those in the control group. Consistent results were obtained in the MWM probe trial which the time spent in the target quadrant was significantly longer in the diving group. Moreover, the diving group spent more time exploring the novel object in the NOR test. Based on the results, we can conclude that 60 days VDE significantly improves hippocampus-dependent learning capacity in trained rats.
Antifungal Activity of Morinda citrifolia leaf extracts against Colletotrichum acutatum Oktira Roka Aji; Larasati Haliimah Roosyidah
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.12009

Abstract

Anthracnose is a plant disease that can infect a variety of plants worldwide. Fungal pathogen groups are the cause of anthracnose, one of which is Colletotrichum acutatum. Morinda citrifolia is famous for having antimicrobial activity. This study aims to determine the antifungal activity of M. citrifolia leaves extract against the growth of the fungus C. acutatum. The extract solvent used was 96% ethanol. The experiment consisted of five treatments (0%, 20%, 40%, 60% and propineb 0.1% as positive control). The method used in this study was the poisoned food technique. In this technique, C. acutatum was grown on PDA media mixed with M. citrifolia leaves extract. Antifungal activity was observed based on reduced mycelium growth compared to control. Observations were made every day by measuring the diameter of the fungus mycelium for eleven days. The analysis showed that 60% M. citrifolia leaves extract effectively inhibited the growth of the mycelium C. acutatum on the eleventh day of observation.
Beak Line and Eye Alignment as Phenotypic Sexing for Domestic Canaries (Serinus canaria) Afif Muhammad Akrom; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Trini Susmiati; Alfarisa Nururrozi; Slamet Raharjo; Rief Ghulam Satria Permana; Puveanthan Nagappan Govendan
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11115

Abstract

Phenotypic sexing of birds is a common practice among the songbird-keeping community, yet it is based on non-reputable information. This study aims to determine the sex of canaries (Serinus canaria) based on the alignment of the eye with the beak line. A total of 26 samples, consisting of six samples of one-month-old canaries (three males and three females based on PCR examination), 20 samples of six-month-old canaries (ten proven breeding pairs) were used in this study. The birds' heads were photographed from the sides, and then the positions of the eyes were compared with the shadow alignment of the beak. The results provided that five young birds and ten adult birds have a beak line alignment under the eye, while ten adult birds and one young bird have a beak line alignment across the eye. The accuracy of sexing using this method was 100% for both female and male adult canaries. However, this result could not be applied to sample that are not yet sexually mature, as two young male birds were found to have beak alignment below the eye. It can be concluded that sexing adult canaries can be performed by observing the eye and beak line's alignment.
Synergism of Phage фPT1b and Antibiotics for Reducing Infection of Escherichia coli Erlia Narulita; Gerda Permata Aji; Bevo Wahono; Siti Murdiyah; Ria Yulian
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11280

Abstract

Foodborne disease caused by Escherichia coli contamination is increasing every year. It also followed by elevating of drug-resistance of E. coli. Bacteriophage can be an alternative for therapy infection.  This study aimed to determine synergism effect of bacteriophage ϕPT1b which has a high rate virulence to E. coli and phage-antibiotics (tetracycline and amoxicillin) synergy. The indigenous bacteria isolates were KR, MJ, KP, PT, PR. Five bacteriophages used namely ϕKR1b, ϕKR2, ϕPT1a, ϕPT1b, and ϕMJ1b. Virulence test was used to determine the ability of each phage in reducing E. coli. Treatment to examine synergism of phage ϕPT1b and antibiotics were P1: amoxicillin, P2: ϕPT1b, P3: ϕPT1b + Amx = 1:1, P4 : ϕPT1b + Amx = 2:1, P5: ϕPT1b + Amx = 1:2, P6 : tetracycline, P2: ϕPT1b, P7: ϕPT1b + Tet = 1:1, P8 : ϕPT1b + Tet = 2:1, and P9: ϕPT1b + Tet = 1:2. The virulence test showed that isolate ϕPT1a with 106 CFU/ml had the highest ability in reducing E. coli. While, the result of synergism test indicated that the synergism of bacteriophage and antibiotics differ significantly (P ≤ 0.05). The best ratios of synergism were 1:1 (ϕPT1b+tetracycline) and 2:1 (ϕPT1b+amoxicilline). In summarize, phage-antibiotic synergy (ϕPT1b with tetracycline/amoxicilline) can reduce the level of antibiotic resistance in isolated E. coli.
New distributional records of Cleome chelidonii L.f. and Cleome rutidosperma DC. (Cleomaceae) in Madura Island Arifin Surya Dwipa Irsyam; Muhammad Rifqi Hariri; Ashari Bagus Setiawan; Rina Ratnasih Irwanto; Asih Perwita Dewi
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.12199

Abstract

Calcareous soil and dry climate are characteristic of Madura Island, located on the east coast of Java, Indonesia. The group of flowering plants that adapted to these conditions is the genus Cleome L. (Cleomaceae). In 1963, Backer and Bakhuizen van den Brink Jr. only listed three species of Cleome from Madura, i.e., C. aspera J.König ex DC., C. gynandra L., and C. viscosa L. Since then, the updated data on the genus Cleome of Madura is not provided yet. Therefore, this study aimed to provide updated information on the genus in Madura island. The botanical exploration was conducted at Bangkalan, Sampang, Pamekasan, and Sumenep in December 2019 to January 2020. The two additional species, namely C. chelidonii L.f. and C. rutidosperma DC., were reported for the first time in Madura. Both species were considered as the newly distributional record for the island. Cleome chelidonii was found in Gapura Tengah (Sumenep Regency), and C. rutidosperma was collected from Kamal (Bangkalan Regency). The botanical information on both species is presented, including the updated key to the Cleome of Madura Island. This study provides updated information on genus Cleome in Madura and reporting the existence of Cleome chelidonii and Clome rutidosperma from the island. The presence of both species was considered as a newly distributional record. 
Determining Phytocomponent of Vetiver Grass (Chrysopogon zizanioides) Under Drought Stress Ani Sulistiyani; Syamsul Falah; Triadiati Triadiati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11686

Abstract

Vetiver grass (Chrysopogon zizanioides, Poaceae) is the leading commodity in Garut Regency, Indonesia, the second-largest producer in the world of vetiver oils. The content of vetiver oils is strongly influenced by the environment, for example, drought stress. Drought stress causes plants to adapt by producing secondary metabolites such as essential oils. This experiment aimed to analyze growth, phytocomponents and obtaining the best quality of vetiver grass accessions under drought stress. The results showed that root and shoot dry biomass were significantly affected by the interaction between drought stress duration and vetiver grass accession. The root dry biomass of Kamojang accession decreased by 25.4%, while Cilawu increased by 5% for 4 days of drought stress. The root length and shoot length were not significantly affected by the treatment. The highest root/shoot length ratio was Verina, and the lowest one was Cisarua. The highest increase in proline occurred in Cilawu accession (85.7%), while the lowest was Verina (6.67%). Essential root oils contain 53 types of phytocomponents, dominated by sesquiterpenes, being khusimol, the highest type. The Cilawu is the best accession based on growth and content rendement.
Enhancing the productivity performance of Cyprinus carpio L. by Manihot utilissima Pohl. leaves supplementation Teuku Reza Efianda; Uci Elfana Sari; Humeira Humeira; Kiki Rishki Ananda
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11510

Abstract

Cyprinus carpio is an economically crucial freshwater commodity in Indonesia and across the globe. Fish demand has a rising trend, which not in line with productivity. Innovation and aspects of food safety are the primary keys to increasing productive capacity. Therefore, the utilization of Manihot utilissima leaves as an alternative to improve national fish's productivity rate is a worthy consideration. They have multiple nutrients, including protein and fat needed by C. carpio seeds. This study aims to determine the optimal dose of M. utilissima supplementation to increase the productivity of C. carpio seeds. The methodology consisted of four treatments (0.5 mL/250 g, 10 mL/250 g, 15 mL/250 g) with four replications for 21 days. The outcome showed that P4 treatment (15 mL) could increase the relative growth rate by 21.10%/day, and it decreased the feed conversion ratio by 0.84. However, there is no significant effect on the survival rate. The study concludes that P4 treatment (15 mL) has positively increased the relative growth rate of C. carpio, and decreased its feed conversion ratio.

Page 20 of 34 | Total Record : 332

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