cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
 6   7   8   9   10 
Molecular Analysis and Effectiveness Assay of AV1 Gene in Transgenic Tobacco for Resistance to Begomovirus Tri Joko Santoso; Muhammad Herman; Sri H. Hidayat; Hajrial Aswidinnoor; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p45-53

Abstract

Genetic transformationof tobacco plant using AV1 gene was conducted atthe previously experiment and generated transgenic tobaccoplants positively carrying the selectable marker nptII gene.The objectives of this experiment were to (1) analyze thepresence of Begomovirus AV1 gene in T0 generation putativetransgenic tobacco plants using PCR technique with specificprimers and its correlation with resistance phenotype, (2)analyze the integration and copy number of the transgene inT0 generation putative transgenic tobacco plants and itscorrelation with resistance response, (3) screen the T0generation putative transgenic tobacco plants with the targetvirus infection and to detect the presence of the virus in thetransgenic plant tissue using universal primers. PCRdetection of AV1 gene in tobacco transgenic was conductedby using specific primer for Begomovirus AV1 gene.Meanwhile, Southern Blot analysis was conducted by usingthe AV1 gene probe. The effectiveness of AV1 gene intobacco transgenic was tested by inoculation of target virususing whiteflies vector. Result of the experiments showedthat there was a positive correlation between the presenceof the AV1 transgene in T0 generation putative transgenictobacco plants and the resistant phenotype. Transgenicplants with a single copy integration of the transgeneexhibited more resistant than the multiple copy one. andnon transgenic plant. The resistance as a result of AV1 geneexpression was indicated with no symptom in T0 generationtransgenic tobacco plants and the accumulation of the virusin the transgenic plants tissue. Northern and Westernhybridization analysis need to be perfomed for investigatingthe presence of mRNA or protein accumulation so that theresistance mechanism of the AV1 gene could be explainedmore detail.
Genetic Mapping of SSR Markers in Eight Soybean Chromosomes Based on F2 Population B3462 x B3293 I Made Tasma; Ahmad Warsun; Dani Satyawan; Saptowo Jumali Pardal; Slamet Slamet
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p69-75

Abstract

Genetic Mapping of SSR Markers in Eight SoybeanChromosomes Based on F2 Population B3462 x B3293. IMade Tasma, Ahmad Warsun, Dani Satyawan, SaptowoJ. Pardal, and Slamet. Aluminum toxicity is one of the maincontrains for cultivating soybean in acid soils. GeneticHak Cipta © 2011, BB-Biogenmapping of SSR markers is one step for detecting aluminumtoxicitytolerant QTLs in soybean. Another step is tophenotype the same population at various aluminum-toxicityenvironments. The objectives of this study were to analyzethe segregation of SSR markers in progenies of an F2population and map the markers in 8 soybean chromosomes.The F2 population was previously developed bycrossing the Al-tolerant parent B3462 and the Al-sensitiveparent B3293. Polymorphic SSR markers in the parents wereused to PCR amplify DNA of the 100 F2 progenies. PCRproducts were separated using agarose or polyacrylamidegels. A Chi-Square test was done with a null hypothesis thatprogenies segregated in a 1 : 2 : 1 ratio. Results showed that125 SSR markers were polymorphics in the parents. Out of125 polymorphic markers, 122 were segregated in theprogenies of the F2 population. Among the segregatingmarkers, 114 were segregated in a 1 : 2 : 1 ratio. Only 8markers (5.6%) did not follow the 1 : 2 : 1 ratio. One hundredand nineteen SSR markers were mapped in 8 soybeanchromosomes. These include 18 markers in chromosomeA2, 10 in B1, 16 (C1), 16 (F), 10 (G), 23 (J), 16 (L), and 10 (N).Total genetic maps covered was 1,194.8 cM with averagemap distances between two adjacent markers of 10.7 cM.Further SSR marker enrichment is required to fill in the gapsof several chromosomal regions. Genetic maps presented inthis study should be useful for detection of Al-toxicitytolerant QTLs in soybean.
Analisis Kekerabatan 50 Aksesi Kelapa Sawit (Elaeis guineensis Jacq.) Asal Kamerun Berdasarkan Marka Mikrosatelit I Made Tasma; Ahmad Warsun; Dani Satyawan; Syafaruddin Syafaruddin; Budi Martono
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p19-27

Abstract

Genetic diversity of theIndonesian oil palm collection remains low and collectionenrichness through exploration activities from the center oforigins is required. In 2009, 103 oil palm accessions fromCameroon were collected at the National Oil Palm GeneticResources Collection located at the District of Sijunjung,West Sumatera. The objectives of the present study were toanalayze the 50 Cameroon-originated oil palm accessions inorder to: (1) determine polymorphism levels of the SSRmarkers used; (2) understand diversity levels of the oil palmaccessions tested; and (3) analyze accessions potentiallyused for germ plasm collection. Fifty oil palm accessionswere used in this study. DNA was isolated from leaves of theselected individual plants representing each of theaccessions. DNA was analyzed with 12 SSR markers. Adendrogram was constructed using the UPGMA throughNumerical Taxonomy and Multivariate System programversion 2.1-pc. Results showed that SSR markers useddemonstrated the average number of alleles per locus of 3.6(2-6). The polymorphism level was 0.53 (0.21-0.73). Thephylogenetic analysis resulted nine clusters with geneticdiversity between two accessions ranged from 4-82%. Tenaccessions (20%) showed low genetic diversity (<10%) butthe accessions demonstrated high diversity in floweringtime. Eleven accessions showed medium diversity level (27-42%). Five accessions demonstrated high genetic diversitylevel (45-82%). A confirmation study using more SSRmarkers is recommended. This study finding may be usefulin planning the oil palm germ plasm collection activities.
Genetic Diversity Analysis of Aluminum-toxicity Tolerant and Sensitive Soybean Genotypes Assessed with Microsattelite Markers I Made Tasma; Ahmad Warsun
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n1.2009.p1-6

Abstract

Analisis Diversitas Genetik Genotipe Kedelai Toleran danPeka Keracunan Aluminium Menggunakan Marka Mikrosatelit.I Made Tasma dan Ahmad Warsun. Persilangandua genotipe kedelai dengan jarak genetik jauh menghasilkanprogeni dengan polimorfisme tinggi pada banyak lokusyang memfasilitasi keberhasilan program pemuliaan dan pemetaankarakter agronomi penting kedelai. Tujuan penelitianini untuk mengetahui diversitas genetik genotipe kedelaitoleran dan peka keracunan aluminium (Al), informasi diversitasalel dan tingkat polimorfisme marka SSR dari genotipekedelai yang diuji, menentukan genotipe dengan jarak genetikjauh sebagai tetua dalam pembentukan populasi pemetaankarakter toleran Al, dan informasi diversitas genetik dalampemilihan tetua untuk program pemuliaan kedelai tolerankeracunan Al. Dua puluh empat genotipe kedelai tolerandan peka keracunan Al dianalisis menggunakan 15 markaSSR. Marka SSR lokasinya menyebar pada 14 kromosom kedelai.Dendrogram dikonstruksi menggunakan UnweightedPair-Group Method Arithmatic (UPGMA) melalui programNumerical Taxonomy and Multivariate System (NTSYS) versi2.1-pc. Diversitas genetik antara dua genotipe kedelai berkisarantara 2-33,2%. Pada diversitas 33,2% uji klaster UPGMAmembagi genotipe menjadi 2 kelompok masing-masing terdiridari 19 dan 5 genotipe untuk kelompok 1 dan 2. Jumlahalel SSR total 81dengan rata-rata jumlah alel per lokus SSR4,4 dan rata-rata tingkat polimorfisme 0,55. Menggunakan diversitastertinggi 33,2% dua genotipe paling peka Al (B3293dan B3442) dari kelompok 1 dan dua genotipe paling toleranAl (B3462 dan B3851) dari kelompok 2 dipilih untuk membentukpopulasi pemetaan karakter toleran Al. Berdasarkannilai diversitas genetik tertinggi 33,2% banyak kemungkinankombinasi persilangan dapat dilakukan antara genotipetoleran Al untuk pemuliaan kedelai toleran Al.
Resistensi Wereng Batang Cokelat Padi, Nilaparvata lugens Stål terhadap Insektisida di Indonesia Sutrisno Sutrisno
Jurnal AgroBiogen Vol 10, No 3 (2014): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n3.2014.p115-124

Abstract

The ricebrown planthopper (BPH), Nilaparvata lugens (Stål) is amajor insect pest of rice and their infestations occur everyyear in several locations in Indonesia. The use ofinsecticides often fails to control the BPH so theirpopulations are still high that cause rice crops showhopperburn and the farmer loses the yields. Thedevelopment of insecticide resistant in BPH population isone of the factors to contribute to the failure of insecticidescontrol. We have detected the development of fieldpopulation BPH resistance to BPMC, carbofuran, MIPC, andimidacloprid, but we do not know yet the development ofresistance to other insecticides to control BPH in Indonesia.This paper will review several cases on BPH resistance toinsecticides in Indonesia and other countries that includeaspects of the development of resistance in the field and inthe laboratory, the mechanism of resistance, inheritance ofresistance, genomics of resistance, and resistancemanagement. A policy and further study is also suggested forinsecticide resistance management in Indonesia.
Konservasi In Vitro Tanaman Jeruk Besar (Citrus maxima (Burm.) Merr.) Kultivar Srinyonya Menggunakan Osmotikum dan Retardan Iswari S Dewi; Gani Jawak; Ika Roostika; Muhammad Sabda; Bambang S Purwoko; Widiati H Adil
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p84-90

Abstract

In Vitro Conservation of Pomelo (Citrus maxima (Burm.)Merr.) cv Srinyonya Using Osmoticum and Retardant.Iswari S. Dewi, Gani Jawak, Ika Roostika, M. Sabda,Bambang S. Purwoko, and Widiati H. Adil. Pomelo is anunderutilized citrus fruit with a potential for commercialization.Only some cultivars have been conserved ex situ, suchas in home yards or in botanical gardens. Such collectionsare vulnerable to biotic and abiotic hazards. The goal of theexperiment was to study the effect of osmoticum (sorbitol)and retardant (ancymidol) on in vitro growth of pomelo.Four-leaf in vitro shoots of pomelo cultivar Srinyonya wereused as plant materials. Murashige-Skoog (MS) medium wasused as the basal medium for the culture. The trial wasarranged in a completely randomized design with threereplications. The treatments consisting of MS + sorbitol (0,20, 40, and 60 g/l) and MS + ancymidol (0, 1, 3, and 5 mg/l).The results indicated that based on plant height, number ofnew leaves, and visual plant architecture, sorbitol treatmentsfrom 20-60 g/l retard the growth of the pomelo plant significantly.On the other hand, ancymidol did not inhibit thepomelo growth significantly, but it was a suitable osmoticumfor improvement of in vitro plant vigor, increasing greencolor of leaf, and increasing root initiation. Leaf senescenceof in vitro plants cultured on media containing sorbitol 40and 60 g/l began 20 week after storage. The best medium forconservation of pomelo cv Srinyonya was MS + 20 gsorbitol/l.
Pembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P Toto Hadiarto; Ma&#039;sumah Ma&#039;sumah; Eny I. Riyanti
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p62-68

Abstract

Azospirillum sp. which has the ability for nitrogenfixation and phosphate solubilization may support modernfarming in Indonesia that is mostly dependent on the usageof chemical fertilizer N, P, and K. Genetic quality ofAzospirillum was improved in this research to obtainsuperior characters toward phosphate solubilization so thatit can become more effective in use for farmers. To achievethis goal, Azospirillum was mutated by means ofelectroporation using transposon EZ-Tn5<kan-2>Tnp. Theelectrotransformation resulted in 20 out of 22 transformantstested contained the marker gen (npt). 10, 6 and 4 mutantshave increased, decreased and lost phosphate-solubilizingfunction, respectively. Mutant with elevated phosphatesolubilizingability may be selected further to be utilized asbiofertilizer while others may be useful for identification ofgenes responsible for phosphate solubilization.
Indirect Organogenesis and Somatic Embryogenesis of Pineapple Induced by Dichlorophenoxy Acetic Acid Ika Roostika; Ika Mariska; Nurul Khumaida; Gustaaf A. Wattimena
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n1.2012.p8-18

Abstract

This research aimed to study the effect of 2,4-D,AdS, and basal media to the regeneration of pineapplethrough indirect organogenesis and somatic embryogenesis,and to study the complete event of somatic embryogenesis.Callus formation was induced by 21, 41, and 62 μM 2,4-Dwith addition of 9 μM TDZ. The non embryogenic calli weretransferred onto 4.65 μM Kn containing medium.Embryogenic callus formation was induced on MS or Bacbasal media consisted of N-organic compounds withaddition of AdS (0, 0.05 and 0.1 μM). The embryogenic calliwere regenerated on modified MS medium with addition of0.9 μM IBA, 1.1 μM BA, 0.09 μM GA3 or MS mediumsupplemented with 0.018 mM BA. The result proved that thesingle auxin of 2,4-D was not enough to induce embryogeniccells. Therefore the non embryogenic calli were regeneratedthrough organogenesis. The 21 μM 2,4-D yielded high level ofcallus formation (80%), higher fresh weight (0.2 g/explant)and higher number of shoot (25 shoots/explant in twomonths). Embryogenic calli were produced on N-organiccompounds enriched media. The regeneration mediumsignificantly affected the level of browning, where the MSmedium with addition of 0.018 mM BA yielded lower level ofbrowning. There was an interaction of embryogenic callusinduction medium and regeneration medium to the numberof mature somatic embryos. The embryogenic callusinduction on MS medium enriched with N-organiccompounds and 0.05 μM AdS followed by the regenerationof somatic embryos on MS medium with addition of 0.018mM BA was the best treatment which yielded 17 maturesomatic embryos/explant.
Kultur Apeks untuk Penyediaan Bibit Unggul Tebu Varietas PS864 dan PS881 Deden Sukmadjaja; Yati Supriyati; Saptowo J. Pardal
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p45-52

Abstract

In vitro culturetechniques have become alternative to help overcome theproblems those are often encountered in the provision ofseeds through conventional means. Micropropagationthrough apex culture in sugarcane has several advantages,such as the produced plants have higher genetic stability,high multiplication rate, and more healthy seeds (especiallyvirus-free)., The aims of the the research were to produceseeds of two varieties of sugarcane, namely PS864 andPS881, through apex culture. Laboratory-scale research wasconducted at the Indonesian Center for AgriculturalBiotechnology and Genetic Resources Research andDevelopment (ICABIOGRAD), Bogor, while sowing seedsnursery was done in the Experimental Station of Gowa,South Sulawesi Assessment Institute for AgriculturalTechnology. The experiments consisted of initiation andregeneration of apexes, shoots multiplication, rootinginduction, and acclimatization of plantlets. Research resultsshowed the initiation and regeneration of PS864 and PS881through apex culture could be done on MS basic mediumcontaining 0.5 mg/l BAP. Shoot proliferation of both varietiesincreased in the second subculture. Addition of 1 mg/l BAPinto medium in the second subculture resulted in higheraverage number of shoots than that of 5 mg/l BAP, both forPS864 and PS881. Addition of 1 mg/l and 5 mg/l kinetinshowed no significant differences for shoot numberscompared to that of PS864 in medium containing 1 mg/lBAP. The average number of PS881 shoots in multiplicationmedia containing 5 mg/l kinetin was higher than that of 1mg/l kinetin. Increased concentrations of NAA and IBA from0.1 mg/l to 0.5 mg/l in the MS medium were correlated to theincreased number of roots in PS864 shoots. Meanwhile, onlyincreased concentration of NAA that affected rooting percentageof PS881. Acclimatization showed the percentage ofthe plantlets grown in polybags was higher than that directlygrown in planting bed. The primary seeds (G0) produced inthese experiments were ready to be reproduced again toobtain further stages.
Development and Characterization of F2 Population for Molecular Mapping of Aluminum-Toxicity Tolerant QTL in Soybean I Made Tasma; Ahmad Warsun; Asadi Asadi
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p1-8

Abstract

Keracunan aluminium merupakan salah satukendala utama dalam budidaya kedelai pada lahan masam.Pembentukan populasi F2 merupakan langkah awal yangmenentukan keberhasilan program pemuliaan tanaman. Tujuanpenelitian ini untuk membentuk dan mengkarakterisasipopulasi F2 hasil persilangan tetua toleran dan tetua pekakeracunan Al. Pembentukan populasi dilakukan menggunakanbantuan marka SSR. Dengan marka SSR populasi dapatdibentuk dengan cepat, akurat, dan efisien. Skrining genotipakedelai pada tanah masam kahat hara menghasilkan duagenotipa toleran dan dua peka. Empat persilangan tunggaldibuat untuk mendapatkan benih F1. Tanaman F1 dan F2 diidentifikasimenggunakam marka SSR Satt_070. Dua populasi(B3462 X B3293 dan B3462 X B3442) dipilih berdasarkansuperiotas fenotipa pada lahan masam dan karakteristik molekulerpasangan tetua. Karakterisasi kedua populasi di lapangmenunjukkan transgresiveness luas untuk karakter reproduksiseperti jumlah polong dan berat 100 biji. Ini mengindikasikanbahwa karakter penting lain selain karakter ketahananterhadap keracunan Al potensial untuk dipetakandari populasi ini. Metoda pembentukan populasi ini akan sangatbermanfaat bagi pemulia tanaman khususnya pemuliakedelai untuk meningkatkan efisiensi program pemuliaanketahanan terhadap keracunan Al.

Page 8 of 26 | Total Record : 252

 6   7   8   9   10 

Filter by Year

2005 2021


Reset
Filter By Issues
All Issue Vol 17, No 2 (2021): DESEMBER Vol 17, No 1 (2021): JUNE Vol 16, No 2 (2020): December Vol 16, No 1 (2020): June Vol 15, No 2 (2019): December Vol 15, No 1 (2019): June Vol 14, No 2 (2018): December Vol 14, No 1 (2018): June Vol 14, No 1 (2018): June Vol 13, No 2 (2017): Desember Vol 13, No 1 (2017): Juni Vol 12, No 2 (2016): Desember Vol 12, No 1 (2016): Juni Vol 11, No 3 (2015): Desember Vol 11, No 2 (2015): Agustus Vol 11, No 1 (2015): April Vol 10, No 3 (2014): Desember Vol 10, No 2 (2014): Agustus Vol 10, No 1 (2014): April Vol 9, No 3 (2013): Desember Vol 9, No 2 (2013): Agustus Vol 9, No 1 (2013): April Vol 8, No 3 (2012): Desember Vol 8, No 2 (2012): Agustus Vol 8, No 1 (2012): April Vol 8, No 1 (2012): April Vol 7, No 2 (2011): Oktober Vol 7, No 1 (2011): Jurnal AgroBiogen Vol 7, No 1 (2011): April Vol 6, No 2 (2010): Oktober Vol 6, No 1 (2010): April Vol 5, No 2 (2009): Oktober Vol 5, No 1 (2009): April Vol 4, No 2 (2008): Oktober Vol 4, No 1 (2008): April Vol 3, No 2 (2007): Oktober Vol 3, No 1 (2007): April Vol 2, No 2 (2006): Oktober Vol 2, No 1 (2006): April Vol 1, No 2 (2005): Oktober Vol 1, No 1 (2005): April More Issue