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Dinia R Dwijayanti,
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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 554 Documents
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Reclamation of Post Coal Mining Using Hydroseeding Involving Seeds of Some Local Papilionaceae Rufaidah Nur Baiti; Endang Arisoesilaningsih
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

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Abstract

This study aimed to determine germination rate, growth and morphological adaptation of root, shoot and leaves of some local Papilionaceae seeds sown in a post coal mining from the South Kalimantan using hydroseeding technique. Species used in this study were Desmodium triflorum (L.) DC, Indigofera spicata Forssk, Alysicarpus ovalifolius (Schumach.) J. Léonard, Crotalaria pallida Aiton, Cajanus cajan (L.) Millsp. dan Sesbania grandiflora (L.) Pers. Twenty seeds of each species were mixed with mulches became hydroseeding, sown in the surface of the tailings from South Kalimantan and each treatment was repeated four times. The media were watered periodically to maintain soil field capacity. Seeds germination rate, plant length, leaves number and coverage were observed twice per week. Plants were harvested 37 days after sown (das) to determine root length and nodules number. The results showed that the beans of D. triflorum, I. spicata, C. pallida and S. grandiflora succesfully germinated and grew in the media. Otherwise the beans of A. ovalifolius and C. cajan failed to germinate. Four germinated species grew variably depend on their specific life form. These Papilionaceae adapted well to the selected medium and showed an intensive root penetration to the tailing layer. Besides D. triflorum, I. spicata and C. pallida were capable to produce some nodules.   Keyword : Adaptation, post coal mining, Papilionaceae, revegetation
Temporal Distribution of Bird Species in the Sarinah Island Porong Sidoarjo Vendy Adrinanda; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 6 (2013)
Publisher : University of Brawijaya

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Abstract

Temporal Distribution of Bird Species in the Sarinah Island Porong Sidoarjo Vendy Adrinanda, Nia Kurniawan Department of Biology, Faculty of Mathematics And Natural Sciences Brawijaya University Birds are members of the vertebrate animals that have feathers and wings. It has given many useful in the human life, as such as many birds species and population in the nature can be used for tourist area. This study aims to determine the temporal distribution and bird species in the Sarinah island. This research use point count combination methode. Observation is done by explored island’s area which will be used as research’s location, and then devide the area with GPS become 4 location point (1,2,3,4). Observation is done 3 times: first, in the morning at 6.00-7.00 o’clock; second, in the afternoon at 12.00-13.00 o’clock; and third, in the evening at 15.30-16.30; during four days that’s two days between 10th-15th Javanese month and two days between 25th-30th Javanese month. Birds species which be found in every location point must be identified and administrated as field data. The identified methode is by taken the birds photoes for every species, count their number and administrate this data into observation data, birds species idetified by literature examination. Spreading map bird species and location that has found to tabulated into microsoft excel and then it is managed in Quantum GIS. Result of the research shows that has been found 44 bird species and divide 24 families. Species that often be found with many individual amount is walet linchi (Collocalia linchii). The bird species often be found in a great number beside 10-15 Javanese month more than beside 25-30 Javanese month, because beside 10-15 javanese month is tides occur and beach to be receding. And according their spreading status, can be devided into 33 species domestic and 11 species migrant. Keywords: birds, temporal distribution, family, species, distribution status.
Evaluasi Status Trofik dan Pencemaran Bahan Organik di Waduk Lahor Malang Menggunakan Bioindikator Diatom Dwie Zesta Viani; Catur Retnaningdyah
Biotropika: Journal of Tropical Biology Vol 6, No 1 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2018.006.01.4

Abstract

Aktivitas keramba jaring apung (KJA) dapat mengakibatkan penurunan kualitas air. Tujuan penelitian ini adalah menentukan dampak aktivitas KJA di Waduk Lahor terhadap perubahan Kualitas air berdasarkan beberapa paremeter fisika kimia dan indeks biotik dari Diatom sebagai bioindikator. Pengambilan sampel air dan Diatom dilakukan di muara Sungai Lahor (3 stasiun) merupakan inlet dari Waduk Lahor dan daerah sekitar bendungan (1 stasiun) menggunakan purposive random sampling berdasarkan aktivitas KJA dengan pengulangan pada masing-masing stasiun tiga kali. Pengambilan sampel Diatom dengan water sampler vertikal dan disaring menggunakan plankton net. Identifikasi dan perhitungan Diatom dengan Counting Chamber Sedgewick Rafter Cell dan buku identifikasi. Hasil identifikasi dan penghitungan Diatom digunakan untuk menentukan Trophic Diatom Index (TDI) dan Pollution Tolerant Value (% PTV). Karakter fisika kimia air (pH, kecerahan, turbiditas, DO dan BOD) diukur pada lokasi yang sama dengan pengambilan sampel Diatom. Hasil penelitian menunjukkan bahwa kualitas air terutama parameter BOD dan kecerahan di Waduk Lahor belum memenuhi standar untuk aktivitas perikanan. Aktivitas KJA di muara Sungai Lahor berdasarkan beberapa indek biotik dari Diatom telah berdampak pada penurunan kualitas air yaitu status nutrisi menjadi eutrofik sampai hiper-eutrofik (TDI) dan tingkat pencemaran bahan organik ringan sampai sedang (% PTV).
EXPLORATION NON-SYMBIOTIC NITROGEN FIXING BACTERIA PRODUCED IAA (INDOLE ACETIC ACID) AND PHOSPHATE SOLUBILIZATION FROM APPLE’S TREE RHIZOSPHERE IN BATU, EAST JAVA Ratna Fadhilah Israwan; Tri Ardyati; Suharjono Suharjono
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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Abstract

Biofertilizer is fertilizer contain microbes that help provide available nutriens for plants. Non symbiotic nitrogen fixing bacteria has been widely utilized as a biofertilizer agent. The objective of this research was to explore bacteria have ability in fixing nitrogen, producing IAA (indole acetic acid) and solubilizing phosphate from rhizosfer of Apple tree in Batu City. East Java. Isolation of soil sample from Apple tree rhizosphere was carried out using serial dilution. Nitrogen fixation ability was assayed qualitatively using nitrogen free bromothymol blue (Nfb) medium enriched with tryptophan. Quantitative measurement of Nitrogen fixation was done by Visocolor ammonium alpha detection kit. IAA production was observed in Luria Bertani medium enriched with tryptophan and Salkowski reagent. Detection of phosphate solubilization was done using Pikovskaya agar and Mo-blue reagent. Four isolates were obtained, isolates TR1, TR2, TR4 and TR5. All isolates have ability to fix nitrogen and to produce IAA. Isolate TR5 has the highest ability of nitrogen fixing (1 mg/L). Isolate TR1 produce maximum IAA concentration (793,55 µg/mL) at 48 hours. Isolate TR4 has the highest ability to solubilize phosphate (31,28 ppm) with index of phosphate solubization 1,21. Isolate TR1, TR4 and TR5 are potential as biofertilizer agents. Keywords: Biofertilizer, IAA, nitrogen fixation, phosphate, rhizosphere  
KAJIAN NUTRIGENOMIK: PENGHAMBATAN Igf-1 PADA ADIPOGENESIS JARINGAN LEMAK VISERAL TIKUS DENGAN EKSTRAK KULIT RAMBUTAN Rizky Nurdiansyah; Sri Rahayu Lestari; Fatchiyah Fatchiyah
Biotropika: Journal of Tropical Biology Vol 1, No 6 (2013)
Publisher : University of Brawijaya

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Abstract

Tujuan penelitian adalah untuk mengetahui dan menjelaskan pengaruh dari ektrak kulit buah rambutan terhadap adipogenesis jaringan lemak viserall tikus dengan melihat ekspresi igf-1. Tahapan penelitian yang dilakukan adalah ekstraksi kulit buah rambutan, perlakuan pada tikus, isolasi protein lemak viserall, separasi protein dan western blot. Tikus strain wistar jantan dibagi menjadi tikus berat badan normal dan obesitas. Perlakuan yang dilakukan antara lain kontrol, asam elagat, placebo, dan perlakuan ekstrak dengan dosis 5, 10, 15, dan 20 mg/kg berat badan. Hasil menunjukkan bahwa tren penurunan berat badan terjadi pada grup tikus obesitas dengan asupan pakan yang tidak berbeda signifikan (P>0,05). Dosis efektif terlihat pada perlakuan 10 mg/kg dan pada dosis 20mg/kg justru terjadi kenaikan berat badan untuk kedua jenis grup tikus. Profil protein antara tikus normal dan obesitas menunjukkan pola yang hampir sama dengan perbedaan intensitas tiap pita. Ekspresi igf-1 terpaut dengan ekspresi igfbp-1 pada 36,7 kDa dan terjadi pada seluruh perlakuan dengan perbedaan nyata pada kedua grup. Ekstrak kulit buah rambutan dengan dosis efektif 10mg/kg memiliki efek anti-obesitas pada tikus obesitas. Menariknya, ekspresi igf-1 menurun pada perlakuan 20mg/kg berat badan dibandingkan dengan kelompok lain. Hal ini diduga penghambatan obesitas tidak melewati jalur tyrosin kinase pada IR family.
Study of Reptile and Amphibian Diversity at Ledok Amprong Poncokusumo, Malang East Java Luhur Septiadi; Berry Fakhry Hanifa; Ainul Khatimah; Yunita Indawati; Muhammad Zakaria Alwi; Muhammad Prayogi Erfanda
Biotropika: Journal of Tropical Biology Vol 6, No 2 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2018.006.02.02

Abstract

Malang is one of the areas that have a high diversity of reptiles and amphibians because of the strategic ecosystem but still minimal in terms of research publications. The purpose of this study was to know the diversity of reptiles and amphibian through the existence of herpetofauna species, microhabitat and indicator species. Data was collected during the rainy season, once a month in the period of three months from October 2017 to January 2018 at night. Data collection was conducted using the Virtual Encounter Survey method with different zones and limited by time. The collected specimens were identified with the guidance of literatures. The collected specimens were then preserved at the Animal Physiology Laboratory, Department of Biology, State Islamic University of Maulana Malik Ibrahim Malang. Then, the data was analyzed to obtain the diversity index, frequency and dominance. The result showed that the number of reptiles found in the location site was seven species consisting of Famili Gekkonidae, Scincidae, Agamidae and Elapidae. Mean while for the species of amphibians, it was obtained nine species consisting of Famili Bufonidae, Ranidae, Rhacophoridae, Dicroglossidae, and Microhylidae. The ecosystem consisted of four habitat types including terrestrial, arboreal, semi-aquatic and aquatic. However, based on the value of diversity index, relative frequency, and dominance value, as well as the indicator species, this area has a relatively low diversity, the disturbed environment and therefore conservation efforts need to be undertaken.
Pengaruh Pemberian Ekstrak Etanol Daun Sirsak (Annona muricata Linn.) terhadap Peningkatan Jumlah Sel T CD4+ dan CD8+ pada Timus Mencit (Mus musculus) Linda Kartika Dewi; Sri Widyarti
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : University of Brawijaya

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Abstract

Imunomodulator merupakan suatu senyawa yang dapat mempengaruhi sistem imun humoral maupun seluler. Daun sirsak (Annona muricata) banyak dikenal sebagai tanaman berkhasiat obat karena mempunyai senyawa-senyawa aktif yang berperan sebagai agen imunomodulator. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun sirsak terhadap peningkatan jumlah sel T CD4+ dan CD8+. Pengekstrakan daun sirsak dengan metode maserasi menggunakan etanol 95%. Selanjutnya ekstrak etanol daun sirsak yang telah dilarutkan dengan NaCMC 0.5% diberikan pada mencit secara oral selama 2 minggu dengan dosis 0, 25, 50, 100 mg/kg BB.. Setelah 2 minggu, timus diisolasi sel-sel limfositnya dan dilakukan perhitungan jumlah sel dengan Flowcytometry dan Haemocytometer. Hasil penelitian menunjukkan bahwa pemberian ekstrak etanol daun sirsak (Annona muricata) dapat meningkatkan jumlah sel T CD4+ dan CD8+ pada timus secara signifikan (p<0.05) pada dosis 25 mg/kg BB. Sel T CD4+ mengalami peningkatan sebesar 75% (3.6 juta sel) dan sel T CD8+ mengalami peningkatan sebesar 238% (3.1 juta sel).   Kata kunci: dosis, ekstrak etanol, daun sirsak, sel T CD4+, sel T CD8+
Effectivity Combination of Bitter Melon (Momordica charantia) and Bitter (Andrographis paniculata) Extract to Suppress Proinflammatory Cytokines in Diabetic Mouse Models Firda Agustin; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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Abstract

nsulin Resistance (IR) is main characteristic of Type 2 Diabetes Mellitus. IL-6 and IFNγ play important role in the deterioration of this disease. The aim of this experiment was to know effectivity combination of Bitter Melon and Bitter to decrease the expression of IL-6 and IFNγ in Type 2 Diabetes Mellitus. Type 2 Diabetes Mellitus were induced by injecting streptozotocin to neonate BALB/c mice in the age of five days (100 mg/kg BW). This experiment applied five groups which divided into  normal group, T2D (Type 2 Diabetes Mellitus model group), T2D-D1 (Bitter Melon dose 5,6 mg/kg BW and Bitter 20 g/kg BW), T2D-D2 (Bitter Melon doses 56 mg/kg BW and Bitter doses 200 mg/kg BW) and T2D-D3 (Bitter Melon doses 5600 mg/kg BW and Bitter doses 20000 mg/kg BW). Relative number of proinflammatory cytokines were analyzed using One-Way ANOVA with SPSS 16.0 for Windows. The results showed that the treatment with Bitter melon (Momordica charantia) and bitter (Andrographis paniculata) gave different effect compared to T2D groups. Medicinal herbs groups supressed proinflamatory cytokines such as IL-6 and IFNγ. So that relative number of IL-6 and IFNγ in treated group is lower than Type 2 Diabetes Mellitus (T2D) mice model.   Key words: IFNγ, IL-6, Insulin, Proinflammatory
Diversitas Arthropoda Tanah di Lahan Kebakaran dan Lahan Transisi Kebakaran Jalan HM 36 Taman Nasional Baluran Mustofa Halli; I Dewa Agung; Bagyo Yanuwiadi
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Abstract

Aim of this experiment to determine arthropods diversity at HM36 Baluran National park after burned and to know it relation with human activity. This experiment held in November 2013. Taking sample using pit fall trap methods at burned land and transition land after burned with two sampling spot and three pit fall jam for each spot. Sample identified with morphological analyze and abundance measurement, Frequency, relative abundance, frequency abundance, and  importance value rank. Diversity analyze using Shannon-Wiener index and Bray Curtis Index to determine similarity level for each location. After burned land has 2.05 and 1.04 at transistion land after burned from diversity analyze. Formicidae dominating at every location of sampling. Each location is different each other with only 25% of Bray Curtis analyze measurement. Abiotic and Colony domination effecting diversity level and environment condition.   Keyword : Pit fall trap, Importance value rank, Shannon wiener index, Bray Curtis index
The Predicted Distribution of Javan Munia (Lonchura leucogastroides) in Indonesia based of Behavior Analysis in Kalibaru, Banyuwangi, East Java Agung Sih Kurnianto; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : University of Brawijaya

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Abstract

Javan Munia is one of the common species of birds that found in west area of Indonesia (Sunda land). This species is endemic to Indonesia (Lombok, Bali, Java, and South Sumatra). The large population, especially during the harvest season makes this bird became a major pest for agriculture. This research aims to determine the adaptive behaviors of Javan Munia that become major reasons of widely spreads and the influence of these factors to the distribution. Observations made in Kalibaru, Banyuwangi, East Java, which is a mostly agricultural area. Any form of behavior in the territorial area (nesting and courtship areas) and range area (where colonize and feed) were observed periodically in the past 12 months. Sunbathing is known as a activity that undertaken by many species of birds, including the Javan Munia. This munia has specific mating and nest construction behaviour. The development of Javan Munia is very quickly, in 40 days, child will become a new adult. Javan Munia is very easy to adapt to the surrounding environment, including the used of oil palm (Elais guineensis) fiber and paper receipts as nest material. There are a possibility of invasive occurrence to the entire island of Sumatra by this species. It is quite possible because of the opening of oil palm plantations and settlements continue, until Aceh. The spread possibilty to east end of distribution (Lombok) can occur up to Nusa Tenggara Island, because of short distances between the islands and similiar vegetation circumstances.

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