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INDONESIA
Indonesian Journal of Cancer Chemoprevention
Published by Indonesian Society for Cancer Chemoprevention
ISSN : 23558989     EISSN : 20880197     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Indonesian Journal of Cancer Chemoprevention (IJCC) is an open access, peer-reviewed, triannual journal devoted to publishing articles on Cancer Chemoprevention including Experimental and Clinical Pharmacology, especially concerning Anti-Oxidants, Anti-Aging, Anti-Inflammation, Anti-Angiogenesis, and Anti-Carcinogenesis; Cancer Detection; Stem Cell Biology; Immunology; in vitro and in silico Exploration of Chemopreventive Mechanism; and Natural Products.
Arjuna Subject : -
Articles 339 Documents
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Phytochemical Screening and Toxicological Evaluation Using Brine Shrimp Lethality Test (BSLT) of Some Fractions of Prasman Leaves (Eupatorium triplinerve V) Extract Shirly Kumala; Dwi Windi Sapitri
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss1pp193-197

Abstract

Prasman leaves (Eupatororium triplinerve V) was well documented to have anti cancer benefit in Indonesian traditional medicine history. However, there were no scientific studies including toxicological assessment on the plan extract. The purpose of this study was to investigate the toxicological effect of some fractions of Prasman leaves methanol extract. Phytochemical screening by the Farnsworth method on powder and some fraction of the methanol extract were conducted followed by toxicity test using the “Brine Shrimp Lethality” test (BSLT) method. In the current study results, the phytochemical screening showed the presence of flavonoid, saponin, coumarin, tannin, steroid and volatile oil. LC50 of the n-hexane fraction 238.66 µg/mL, ethyl acetate fraction 24.42 µg/mL,  and n-butanol 64.10 µg/mL.Keywords : BSLT, Toxicity test, Eupatororium triplinerve
Red Betel Leaves Methanolic Extract (Piper crocatum Ruiz & Pav.) Increases Cytotoxic Effect of Doxorubicin on WiDr Colon Cancer Cells through Apoptosis Induction Nindi Wulandari; Argandita Meiftasari; Hilyatul Fadliyah; Riris Istighfari Jenie
Indonesian Journal of Cancer Chemoprevention Vol 9, No 1 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss1pp1-8

Abstract

Doxorubicin is a chemotherapeutic agent that causes a lot of side effects in high doses. Thus, combination with a co-chemotherapeutic agent which can increase its toxicity on cancer cells, is needed to reduce its therapeutic dose. Red betel leaves (Piper crocatum Ruiz & Pav.) have been known to contain flavonoids and alkaloids that have anticancer activity. This study was conducted to determine the cytotoxic effect and apoptosis induction of red betel leaves methanolic extract (RBM), doxorubicin (dox) and the combination of them on WiDr cells as model of colon cancer. RBM was extracted by soxhlet method using methanol. Cytotoxicity assay was performed using MTT assay for both single and combination treatments for 24 hours to determine IC50 and CI as their parameters. Apoptosis induction was analyzed by double staining method using ethidium bromide-acridine orange staining. Treatment of RBM and dox on WiDr cells for 24 hours showed cytotoxic activity with IC50 100 μg/mL and 1.6 μM respectively. Combination of RBM and dox performed synergism effect with CI<0.9 (p<0.05). Combination of RBM (12.5 μg/mL) and dox (0.4 μM) increased the number of apoptosis cells compared to each single treatment. Based on this study, it can be concluded that red betel leaves methanolic extract is potential to be developed as a co-chemotherapeutic agent of doxorubicin on colon cancer but still need further study to disclose the underlying molecular mechanisms.Keywords :  Red betel leaves (Piper crocatum Ruiz & Pav.), doxorubicin, WiDr cells, co- chemotherapeutic agent
Oyster Mushroom (Pleurotus ostreatus) Inhibits Migration and Metastasis on 4T1 Breast Cancer Cells Lodyta Nawang Tika; Layung Sekar Sih Wikanthi; Shofa Annur; Retno Murwanti
Indonesian Journal of Cancer Chemoprevention Vol 7, No 3 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev7iss3pp99-103

Abstract

Metastasis is the main cause of death among brast cancer patient. Pleorotus ostreatus is known as anticancer agent that inhibits angiogenesis. Ethanolic extract of Pleorotus ostreatus (EEP) which contains lovastatin is predicted to inhibit metastatic cancer through inhibition of MMP-2 and MMP-9. The aim of this study was to determined antiproliferative and anti metastatic activity of EEPw (Ethanolic extract of wet Pleorotus ostreatus) and EEPd (Ethanolic extract of dried Pleorotus ostreatus ) in 4T1 metastatic breast cancer cells line. Qualitative analysis of lovastatin was determined by thin layer chromatography (TLC) using dicloromethan and etil acetat as mobile phase and lovastatin standard. Scratch wound healing assay was used to determine migration inhition ability of EEP while MMP-9 and MMP-2 activity were analysed by gelatine zymography. Molecular docking was performed to know the interaction between lovastatin and MMP-2 & MMP-9. The result showed that EEPw and EEPd contain lovastatin which were proved by spray reaction with anisaldehid. Each of EEPw and EPPd had cytotoxic activity with IC50 760 and 400 μg/mL respectively. Both of them inhibited closure for about 50 % on 4T1 metastatic breast cancer cells line compared to control. Either EEPw or EEPd decreased MMP-9 expression level compared to control. Lovastatin had higher affinity to bond with either MMP-2 or MMP-9 than native ligand. Overall, EEP could be developed as anticancer agent which was targeted on MMP-2 and MMP-9.Keywords : Pleurotus ostreatus, 4T1 metastatic cells, MMP-2, MMP-2, antimetastatic
Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells Yurista Gilang; Adam Hermawan; Aditya Fitriasari; Riris Istighfari Jenie
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev3iss2pp404-409

Abstract

Therapy of colon cancer by using 5-FU often causes problems of resistance. This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation. Either single treatment of hesperidin 200µM or 5-FU 1500 µM did not trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms.Keywords : hesperidin, 5-fluorouracil, WiDr cells, cytotoxic, apoptosis
Inhibitory Effect of Bombay Onion (Allium cepa L.) Extracts on Mitotic Chromosomes Edy Suwarso; Dewi Nur Anggraeni
Indonesian Journal of Cancer Chemoprevention Vol 7, No 2 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev7iss2pp46-48

Abstract

Cancer is one of the world health problems. Free radical could damage cells and lead to cancer. Antioxidant compound could be found in some vegetable such as phenols and flavonoids. Flavonoids and phenol significantly decrease micronucleus formation on mitotic chromosomes. This present study aim to explore the inhibitory effect of Bombay onion extracts (BOE) on mitotic chromosomes induced by cyclophosphamide. Twenty-five mice (body weight 20–35 g) were divided into 5 groups, with 5 mice in each group. Normal control group (NCG) were given CMC 5 mg/mice/7 days. Mutagen control group (MCG) that is previously given as NCG, 4 hours later were given cyclophosphamide at a dose of 50.0 mg/kg bw intraperitoneally. Three treatment groups, group 1 (TG-1), TG-2, and TG-3, were given BOE with doses of 100; 200; and 400 mg/kg bw for 7 days, respectively. After 7 days, all three groups were treated similarly with MCG. Thirty hours later, all groups were put to death and femur bone marrows were analyzed to count the number of micronucleus. The results showed that the number of micronucleus in every 200 polychromatic erythrocyte cells at mitotic chromosomes for NCG is 29.8 ± 2.387, MCG with a value of 120.8 ± 5,718, TG-1 showed 94.8 ± 7.049, TG-2 is 68.8 ± 3.421, and TG-3 which is 30.8 ± 0.837. TG-3 showed a similar result with NCG (p> 0.05).Keywords : mitotic chromosomes, Bombay Onion Extracts, femur bone marrow, micronucleus
The Safety of Areca Seed Ethanolic Extract as Potential Chemopreventive Agent is Proven by Acute Toxicity Test Sri Handayani; Riris Istighfari Jenie; Ratna Asmah Susidarti
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev3iss1pp345-350

Abstract

Areca (Areca catechu L.) seeds ethanolic extract (AE) exhibits antiproliferative activity and induces apoptosis on T47D and MCF-7 cells. This study aimed to verify AE safety using acute toxicity test to support its development as chemopreventive agent. Male Sprague Dawley Rat (Rattus norvegicus) age 8 weeks divided into five groups, one group of control treated with 0.5% CMC-Na only and four groups for treatment. Single dose in oral administration was done to test animal with various dose of AE starts from lowest dose to highest dose expected toxic to all of test animal (0.1; 0.72; 5.36 and 10 gram/kgBW). Observation was done during 24 hours and continued for 14 days. The observation criteria were toxic symptoms, appearance and mechanism of toxic effect and pathology of vital organ. Histopathology analysis of some vital organs was done with Haematoxyllin&Eosin (H&E) staining. Toxic effect did not appear either on treatment groups or control group. Treatment of single dose of areca ethanolic extract, even in highest dose, did not cause the death of the animals. Therefore, observation extended to 14 days and terminated by necroption of the animals. All of groups did not show histopathological alterations in microscopic observation. Category of the potential toxicity of AE is practically non-toxic, ie 10 g/kgBW. The result shows the safety of areca seed ethanolic extract which is important for its development as chemopreventive agent.Keywords: Areca catechu, acute toxicity, rat
Taraxacum officinale Leaves Ethanolic Extract as Immunostimulatory Agent For Reducing Side Effect of Doxorubicin in Sprague Dawley Rats Sri Kasianningsih; Erlina Rivanti; Ratih Hardika Pratama; Nanda Resa Pratama; Muthi&#039; Ikawati; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss1pp135-140

Abstract

Doxorubicin as chemotherapeutic agent causes immunosuppresive. The aim for this study to determine the effect of ethanolic extract of Taraxacum oficinale (ETO) in immunity system of Sprague Dawley rat that induced by doxorubicin to observe the profile of immunity cells. Sprague Dawley rats were divided into five groups each groups contain five rats: control doxorubicin group, doxorubicin dose 4,67 mg/kgBW+ ETO dose 1000 mg/kgBW, doxorubicin dose 4,67 mg/kgBW+ ETO dose 500 mg/kgBW, control extract group, and without treatment. Then the number of leukocytes, lymphocytes and neutrophils were analyzed by hematology analyzer, whereas CD8+ T lymphocytes by flowcytometry. Results showed groups of doxorubicin combined with ETO dose 1000 mg/kgBW and 500 mg/kgBW increased the number of leukocytes, lymphocytes, neutrophils,  cytotoxic CD8 + T cells T cells compared to control doxorubicin group. These data presents that etanolic extract of Jombang leaves has immunostimulatory activity and potential as co-chemotherapy agents. Molecullar mechanism underlaying it’s immune activity need to be explored in detail.Keywords: co-chemotheraphy, doxorubicin, immunostimulatory, in vivo Taraxacum officinale
Secang Heartwood Ethanolic Extract (Caesalpinia sappan L.) Inhibits Mesenchymal Stem Cells Senescence Asri Mega Putri; Nindya Budiana Putri; Rahmawaty Rachmady; Idlohatud Dilalah; Retno Murwanti; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp126-134

Abstract

Antioxidants have the ability to scavenge free radicals, leading to inhibition of cells senescence. Secang Heartwood (Caesalpinia sappan L.) contains flavonoid brazilein, known of its high antioxidant activity. However, the activity of secang as senescent cells inhibitor has not been known. The aim of this study is to explore the potential of Caesalpinia sappan ethanolic extract (CSE) as free radical scavenger, thus inhibits senescent cells. Two concentrations of SE under IC50, 2µg/mL, 5µg/mL and 10µg/mL were applied on Mesenchymal Stem Cells (MSCs) and combined with 5µM of doxorubicin (Dox) as senescence inductor; both of them were compared with MSCs-Dox group. X-gal, chromogenic substrate of senescent cells, was given on MSCs, giving blue stain on senescent cells. To explore brazilein mechanism in senescence inhibition, molecular docking using PLANTS on topoisomerase II was performed. MSCs that treated with 10µg/mL of SE qualitatively showed reduction intensity of blue stain. Number of stained cells also reduced from 76% of MSCs-Dox to 38 % of 10µg/mL SE-Dox group. Docking score shows that brazilein (-86.91) is more stable to interact with topoisomerase II than doxorubicin (-82.46) and has same binding site (Val 174). These findings demonstrate starting knowledge on CSE potential as senescent cells inhibitor through brazilein activity on topoisomerase II.Keyword: Caesalpinia sappan L., Senescence, β-galactosidase, Molecular Docking
The Effect of Co-Administration of Telang Leaves Ethanolic Extract Towards Fluoxetin’s Sedative Effect on Male Balb/C Mice Based on Sleeping Duration Parameter Anif Nur Artanti; Maria Ulfah
Indonesian Journal of Cancer Chemoprevention Vol 6, No 1 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss1pp7-11

Abstract

One of Telang plant’s advantages is its activity as sedative agent. Previous studies indicate that Telang plant has anticonvulsant activity on mice at certain dose. This study was conducted to find out the sedative effect of Telang plant, particularly its leaf, when being co-administered with fluoxetin. Phytochemical analysis was carried out qualitatively on Telang ethanolic extract leaves (TEE) to find out the content of chemical compound first.This study was an experimental research with post-test only control group design, employing male balb/c mice. The effect of Telang ethanolic extract co-administration with fluoxetin was observed. The parameter being used in the study was the duration of sleep. Analysis was done by comparing mice sleeping duration prior to administration of fluoxetin alone and in combination with Telang ethanolic extract. The data was then analyzed using SPSS 17.0 for Windows.The results showed that TEE contained tannin, saponin, and flavonoid compounds, and co-administration of TEE and fluoxetin at various doses could provide sedative effect on mice. The co-administration of 400 mg/kgBW extract and 15 mg/kgBW fluoxetin could provide the mean sleeping duration 43 minutes longer than positive control.Keywords:  telang leaves ethanol extract, fluoxetin, sleeping duration
The Cytotoxic Activity of Solanum Nigrum Ethanolic Extract on Widr Human Colon Cancer Cells Astrid Ayu Maruti; Ilham Augusta F.; Dyaningtyas Dewi Pamungkas Putri; Adam Hermawan; Muthi&#039; Ikawati
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp291-294

Abstract

Solanum nigrum L. or Leunca in Indonesia has been traditionally used as a herbal plant, which is believed to have anti-tumor properties, although the mechanism for the activity remains unknown. The resecarch aim to examine the cytotoxic effect of the ethanolic extract of Solanum nigrum on WiDr human colon cancer cells. In this study, we prepared an ethanol extract from herb of Solanum nigrum and investigated the mechanism involved in its growth-inhibitory effect on WiDr human colon cancer cells. Herbs of Solanum nigrum dry powder is extracted with 70% ethanol then added into the WiDr cell culture in 96 wells plate in various concentration : 50, 100, 250, and 500 µg/ml. Cytotoxicity of the Solanum nigrum ethanolic extract  was analyzed with MTT assay on WiDr human colon cancer cell lines. Results from the MTT assay showed WiDr cells was weakly suppressed in the presence of the extract. The result of the assay also showed a very close correlation between the Solanum nigrum extract concentration and the surviving cell numbers which means the extract caused cell death in a dose-dependent fashion in WiDr cancer cells with the IC50 of 359,23 µg/ml. Collectively, the research suggest further studies to explore other chemopreventive possibilites of Solanum nigrum ethanolic extract.Keywords : colon cancer, MTT assay, cytotoxic, WiDr, Solanum nigrum

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