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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

143 144 145 146 147

Purification of neuraminidase from Influenza virus subtype H5N1 Simson Tarigan; Risa Indryani; Darminto .; Jagoda Ignjatovic
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Influenza-virus neuraminidase plays vital role in the survival of the organisms. Vaccination of animals with this glycoprotein confers immune responses so that enable it to protect the animals from incoming infection. Supplementation of conventional vaccines with this glycoprotein increases the protection and longevity of the vaccine. Purified neuraminidase can also be used to develop serological tests for differentiation of serologically positive animals due to infection or to vaccination. In this study purification of neuraminidase from influenza virus subtype H5N1 was described. Triton x-100 and Octyl β-D-glucopyranoside were used to extract and diluted the glycoprotein membrane. The enzymatic activity of the neuraminidase was assayed using a fluorochrome substrate, 4-methylumbelliferyl-a-D-N-acetyl neuraminic acid, which was found to be simple, sensitive and suitable for the purification purpose. The neuraminidase was absorbed selectively on an oxamic-acid agarose column. The purity of neuraminidase eluted from this affinity column was high. A higher purity of the neuraminidase was obtained by further separation with gel filtration on Superdex-200. The purified neuraminidase was enzymatically active and did not contain any detectable haemagglutinin, either by haemagglutination assay or by monospecific antibodies raised against H5N1 hemagglutinin. The purified neuraminidase was recognized strongly by antibodies raised against an internal but only weakly by that against C-terminal regions of the neuraminidase protein of H5N1-influenza virus. The purified neuraminidase was in tetrameric forms but dissociated into monomeric form on reducing condition, or mostly dimeric form on non-reducing SDS-PAGE. Key Words: Neuraminidase, Influenza, H5N1, Methylumbelliferyl, Oxamic-acid

Effect of Collection Technique on Yield of Bovine Oocytes and the Development Potential of Oocytes from Different Grades of Oocytes R.G Sianturi; M Thein; H Wahid; Yono C Rosnina
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 3 (2002): SEPTEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Oocyte collection technique is important to obtain a maximum number of oocytes to be employed on in vitro production of embryos. In this study, immature bovine oocytes were collected from slaughterhouse ovaries by two techniques: aspiration of 2- to 6-mm follicles and slicing. Following collection, oocyte qualities were classified into four categories (A, B, C, and D) on the basis of cumulus attachment. Oocytes of each category were matured in vitro in CO2 incubator for 22-24 hours and cumulus expansion and maturation rates were observed. The total number of oocytes (group A+B+C+D) and yield of good quality oocytes (only group A and B) recovered per ovary by aspiration were 12.02 and 8.21, and by slicing were 29.38 and 19.65 (P<0.01), respectively. The total cumulus cells expansion rates of A, B, C and D oocytes were 97.1%, 88.3%, 6.0% and 20.6% respectively. Maturation rates for A, B and C categories of oocytes were 91.4%, 82.3% and 35.0% respectively while no matured oocyte was observed for group D oocytes. Maturation rates were significantly different between group A and C and also between B and C but not between A and B (P<0.05). In conclusion, slicing technique recovered more oocytes per ovary (2.4 times) than that of aspiration and the best maturation rate was observed from category A oocytes which surrounded by more than 3 layers of cumulus cells. However oocytes of category A and B can be considered as good quality oocytes. Key words: Collection technique, aspiration, slicing, oocyte quality, maturation

Supplementation of corn-soybean based layer diets with different levels of acid protease Satrijo Widi Purbojo; Edwin S Luis; Reny Debora Tambunan; Dicky Pamungkas
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of this research was held in Institute of Animal Science farm, University of the Philippines Los Banos, was to know the effects of acid protease supplementation in layer diets. Ninety-four-week old pullets were caged individually. Five treatments were randomly arranged to 95 pullets following a completely randomized design. Each treatment was replicated 19 times. The feeding trial lasted for 16 weeks. The same management practices were provided to all treatments throughout the feeding period. Pullets were fed once a day in the morning and clean drinking water was available to the pullets at all times. A basal layer diet that contained 18% crude protein (CP) and 2800 Kcal ME/kg supplemented with required vitamins, minerals and amino acids were formulated. The diets with reduced protein of 17% and 2800 kcal ME/kg was also formulated. The diet with the reduced crude protein was supplemented with different levels of protease (0.05, 0.075 and 0.1%). Six birds from each treatment were randomly selected and placed in individual digestion cages. They were fed with their respective diets with chromic oxide as indicator for 7 days. Chromic oxide was added to the different diets at 0.2%. On the 3rd to 6th day of feeding, feces were collected using stainless fecal trays installed under each cage. At the end of the collection period, fecal samples collected from each replicate of treatment were dried then subjected to proximate analysis and chromic oxide determination. Result showed that no significant difference on biweekly feed consumption and overall observations. Reduced CP + 0.1% protease was the highest on the hen day production (93.75%) and the lowest of feed conversion (1.85) while reduced CP + 0.075% protease was the highest of egg weight (58.82 g) and eggshell thickness (0.392 mm). There was no significant difference on digestibility coefficient. Key words: Acid protease, layer diets, supplementation

Effect of katuk leaf meal and mulberry leaf meal in the diet on size and mineral content of tibia bone of laying quail Hermana W; Toharmat T; Sumiati .; Manalu W
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 3 (2013): SEPTEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The objectives of this research was to study tibia bone mineral content and blood profil of laying quail offered control diet (P0); diet with 10% katuk leaf meal /KLM (P1); diet with 10% mulberry leaf meal/MLM (P2); and diet with 5% KLM+5% MLM (P3). A completely randomized design using 4 treatments, 5 replications, and 2 quails of 17 weeks of age in each experimental unit replication was used in this experiment. The data were analyzed using analysis of variance and Duncan test. Parameters observed were tibia bone mineral content. The result showed that quail tibia bone weight and percentage of tibia bone weight were not affected by treatment, but diametre of tibia bone from quail fed mixture of KLM+MLM in the diet shorter (P < 0.05) than that of the other treatment. It was concluded that KLM and MLM can be used in the diet of laying quail up to 10% without affected the tibia bone mineral content. Key Words: Katuk Leaf Meal, Laying Quail, Mulberry Leaf Meal, Tibia Bone.

Effect the administration of human chorionic gonadotrophin (hCG) hormone following superovulation treatment in buffalo Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A numbers of studies had been reported to evaluate the effect of hormone for superovulation treatment in buffaloes, however the numbers of embryo recovered is still very small. One of the limiting factors which affect the numbers of embryo recovered is preovulatery LH concentration. The experiment was carried out to study the effect of hCG following superovulation treatments in buffaloes as an effort to increase the embryo recovered. Ten (10) buffaloes in three different genotypes (Riverine, swamp and its crosses) were superovulated using follicle stimulating hormone (FSH). A total of 12 ml FSH (Folltropin) was injected intramuscularry twice a day with 12 hours interval in a decreasing doses for 4 days (2.5, 2.5; 2.0, 2.0; 1.0, 1.0 and 0.5, 0.5 ml). Prostaglandin was injected intramuscularry 2 days after the first injection of FSH followed by administration of 500 IU hCG two days later. At the same days of administration of hCG, artificial insemination (AI) using frozen semen was conducted and repeated again after 12 and 24 hours. Blood collecting was conducted 3 times a week for obtaining the progesteron level of plasma blood. Embryo was collected by non-surgically technique on day 6 of estrus cycle by flushing each horn of uterus with 500 ml Dubelco's Phosphat Buffer Saline (DBPS). Parameter recorded were diameter of ovary (DO), total corpus luteum (TCL), number of embryo collected (NE), percentage of recovery rate (%RR) and peak progesteron level (PP). Administration of hCG significantly increase the response of buffalo to superovulation treatment. The mean of DO (cm), TCL, NE and RR (%) was 4.0, 6.3, 2.1 and 37.2 and 4.5, 7.5, 3.9 and 48.1 for controll and 500 IU hCG respectively. The mean NE was significantly higher (P<0.05) in hCG than those control. The peak progesterone concentration was highly significant higher (P<0.01) in hCG (8.9 ng/ml) than those control (6.8 ng/ml). Both left and right ovary gave a similar response to superovulation treatments. In conclusion the admission of hCG following superovulation treatments using folltrophin increase the number of embryos recovered. Key Words: hCG, Superovulation, Buffalo, Embryo

Effect of enrichment of organic selenium, inorganic selenium and vitamin E in quail ration on the performances and potency of quail egg as a source of antioxidant Syahrir Akil; Wiranda Gentini Piliang; C. Hanny Wijaya; Desianto Budi Utomo; I Komang Gede Wiryawan
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The change of life style influences human health and it contributes to many human diseases outbreak, therefore nutrition of antioxidant is required. This study was aimed to get an optimum level of combined organic selenium, inorganic selenium and vitamin E in obtaining the best production and reproduction of quails and the highest antioxidant level in quail eggs. This study was conducted from January to August 2008. Numbers of observed quails were 720 individuals (360 female and 360 male quails). The treatments were applied when the quails were six weeks old. Nine treatment diets were: To (commercial diet), T1 (0.46 ppm inorganic Se + 43.50 ppm vitamin E), T2 (0.46 ppm inorganic Se + 87.00 ppm vitamin E), T3 (0.92 ppm inorganic Se + 43.50 ppm vitamin E), T4 (0.92 ppm inorganic Se +87.00 ppm vitamin E), T5 (0.46 ppm organic Se + 43.50 ppm vitamin E), T6 (0.46 ppm organic Se + 87.00 ppm vitamin E), T7 (0.92 ppm organic Se + 43.50 vitamin E) and T8 (0.92 ppm organic Se 0.92 + 87.00 ppm vitamin E). The design of the experimental applied was a factorial – nested design. Any significant differences among the treatment diets were analysed using Duncan's test. The result of this study indicated that treatment T7 (0.92 ppm organic selenium + 43.50 ppm vitamin E) in general gave the highest content of selenium in meat, in egg albumin, egg yolk, vitamin E in egg yolk, glutathione peroxidase (GSH-Px), activity of antioxidant, hatchability and low mortality. Key Words: Quail, Selenium, Vitamin E, Antioxidant

Bali cattle performance in the dry tropics of Sumbawa Tanda Panjaitan; Geoffry Fordyce; Dennis Poppi
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 3 (2003): SEPTEMBER 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Preliminary data on Bali cattle reproduction and growth presented is based on monitoring between August 2001 and August 2003 in the Sumbawa and Dompu Districts of Sumbawa Island, NTB. Sixty two % of calves were born between May and July inclusively. Inter-calving interval averaged 11.9 ±1.9 months; though recognized as an under-estimate, it still indicated of high fertility. Post-natal calf mortality was 5.3%. Birth, 6-, and 18-month weights of 14.2 ± 2.4 kg, 90 ± 20 kg, 172 ± 40 kg, respectively, were recorded. Average daily gains between birth to 6 months and from 6 to 18 months were 0.41 ± 0.11 and 0.23 ± 0.11 kg/d, respectively. Females reached mature size at 2.5-3.0 years at a mean weight of 237 kg and height of 1147 mm. Weight could be accurately predicted from chest girth: Weight (kg) = 7e0.0023 Chest girth (mm) (R2=0.9656). At the sites monitored, reproduction was high, and growth was typical for Bali cattle, indicating that these animals are well suited to the dry tropical environment of Sumbawa. Key words: Bali, weight, cattle, tropic

In vitro studies: The role of immunological cells in Indonesian thin tail sheep in the killing of the liver fluke, Fasciola gigantica S.E Estuningsih; S Wiidjajanti; S Partoutomo; Spithill .; H Raadsma; D Piedrafita
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Previous studies have shown that Indonesian Thin Tail (ET) sheep exhibit high resistance to challenge with Fasciola gigantica when compared with Merino sheep, and this resistance is expressed in early infection. In order to study the role of the immune system in this resistance to ET sheep, in vitro studies were undertaken in the laboratory. In vitro study to confirm the ability of immune cells from ET sheep in the killing of F. gigantica larvae has been done by incubating immune cells and F. gigantica larvae together with immune sera or normal sera. The viability of the larvae was observed over a period 3 days incubation by observing their motility. The results showed that the cells isolated from F. gigantica- challenged ET sheep in the presence of immune sera from ET were able to kill 70% of the larvae. In contrast, cells from infected Merino were unable to kill a significant number of F. gigantica using the same sera source. It seems that the cytotoxicity was dependent on the presence of immune sera and ET peritoneal cells, suggesting the potential role of an antibody-dependent cell cytotoxic (ADCC) mechanism in the resistant ET sheep.Key words: In vitro, Fasciola gigantica, peritoneal cell, sheep gigantica.

Genetic relationships of Kampung, Pelung, Sentul and Black Kedu Chicken using Microsatellite DNA Markers: I. Lingkage group of macro chromosome Tike Sartika; S Iskandar; L.H Prasetyo; H Takahashi; M Mitsuru
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 2 (2004): JUNE 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Genetic relationships of Kampung, Pelung, Sentul and Black Kedu chickens were studied on the basis of microsatellite DNA polymorphism. DNA samples were analyzed using nine microsatellite markers which chosen from linkage group of macrochromosome (chromosome 1-8) such as, locus ABR 258, ABR359, ABR 297, ABR 339, ABR 75, ABR 209, ABR 28, ABR 419 and ABR 604. Analyses of amplified DNA fragments were performed using Gene Mapper 2.0 software (PE, Applied Biosystems). The allele frequencies in each breed estimated by direct counting. Since all nine microsatellite markers were polymorphic, genetic distance between the breeds could be calculated based on the frequencies of alleles of the microsatellite. Genetic relationships between the breeds could be constructed. The results indicated that a total of 73 allele were detected while typing all the four breeds of local chicken and one breed of White Leghorn as outgroup breed acrossed nine loci. The number of alleles was observed in all of the breed ranged 3-17 alleles according to the microsatellite under scrutiny. Highest observed number of alleles was found in Kampung Chicken 60 alleles (82.2%). The UPGMA method for dendogram based on Nei genetic distances indicated that the local chickens have the same of ancestor, while Kampung and Sentul chicken have the same cluster followed by Black Kedu and Pelung Chicken. Key words: Local chicken, microsatellite, macrochromosome, genetic distance

Effect of level of lactic acid bacteria inoculant from fermented grass extract on fermentation quality of king grass silage M.A Antaribaba; N.K Tero; B. TJ. Hariadi; B. Santoso
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 4 (2009): DECEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Ensiling is a method of preserving moist forage based on natural fermentation where lactic acid bacteria (LAB) ferment water soluble carbohydrate into organic acids mainly lactic acid under anaerobic condition. The aim of this study was to evaluate the quality of king grass (Pennisetum purpureophoides) ensiled with addition of LAB prepared from fermented grass extract (LBFG). Four treatment were (G0) king grass without additive; (G1) king grass with 2% (v/w) of LBFG; (G2) king grass with 3% (v/w) of LBFG; (G3) king grass with 4% (v/w) of LBFG. Ensiling was conducted in bottle silos of 225 g capacity at room temperatures (27.0 ± 0.20C) for 30 days. The results showed that crude protein content in silage G1, G2 and G3 were relatively higher than that in silage G0. The pH value, butyric acid, total VFA and NH3-N concentrations decreased linearly with increasing level of LBFG addition, while lactic acid concentration increased linearly with LBFG addition. It was concluded that addition of 3% (v/w) of LBFG resulting a better fermentation quality of king grass silage than 2% and 4% (v/w) of LBFG. Key words: Silage, Lactic Acid, Fermentation, King Grass

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Ahmadi Riyanto

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Journal Mail Official ahmadi_puslitbangnak@yahoo.com

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA