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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

144 145 146 147 148

Indonesian avian influenza viruses character in second wave epidemic N.L.P.I. Dharmayanti; R Indriani; R Damayanti; A Wiyono; R.M.A. Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Second wave of epidemic avian influenza occurred from December 2004 until April 2005. In March 2005, the disease had infected some districts in South Sulawesi such as Wajo and Sopeng. More than 21 field isolates have been collected and identified as avian influenza virus subtype H5N1. In this study further characterized was undertaken for 14 isolates of avian influenza using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and sequencing in region of HA1 gene. It was then followed by genetic analysis to identify the mutation and phylogenetic relationship of the isolates. The study indicates that the Indonesia isolates collected in second wave epidemic are generally having a different group to the isolates group in 2003 and 2004. There is point mutation in the nucleotide sequence of the isolate collected at August 2004-March 2005, that is the replacement of adenine by guanine in the position of 195. Key Words: Avian Influenza Virus, Second Epidemic Wave, Mutation

Nitrogen kinetics in growing sheep consuming Leucaena leucocephala, Gliricidia sepium or Calliandra calothyrsus as a sole diet Yeni Widiawati; M Winugroho; E Teleni
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The utilization of protein feed by animals are influenced by the process of its degradation in the rumen and its digestion in the intestine. The extent of its degradation and digestion are influenced by the form of protein and the present of secondary compound in the plant. The aim of the study was to determine the amont of protein in the three leguminouse shrubs utilized by the animals. The study on the utilization of protein from leguminouse shrub was undertaken by investigate the nitrogen kinetic in 24 head of growing sheep (BW 23kg) consuming Leucaena leucocephala, Gliricidia sepium, Calliandra callothyrsus leaves and Rhodes grass hay. The diets were fed every two hours starting at 07:00 am. The animals were divided into four groups of dietary treatment namely LL, GS, CC and CG groups which were fed by Leucaena, Gliricidia, Calliandra dan Rhodes grass leaves, respectively. The Rhode grass, as control diet, was mixed with 1.4% of urea to increase the nitrogen offered to the animals up to 150 g/day. Thus all the four diets were offered the same amount of nitrogen/day. Measurements were undertaken on feed utilization and nitrogen kinetics in experimental animals. The amount of nitrogen consumed then undegraded in the rumen as well as undigested in the intestine was significant higher in CC group (P < 0.01) then those in LL, GS and CG groups. The value was 67% for CC group then followed by 35% for LL group and 33% for GS group. The amount of nitrogen degraded in the rumen thus lost in urine was significantly higher in animals fed GS and LL leaves compared to those in animals fed CC diet (P < 0.01). The values were 46% for GS and LL group and only 16% for CC group. The amount of nitrogen retained by the animals was similar among the three groups being 17-21% (P > 0.05). The Plasma urea concentration, urea entry rate and urea in urine animals were similar in LL and GS groups but higher than those in CC group. The values were 0.86; 0.8; 0.46 mg/ml (P < 0.01) and 79; 78; 50 g/d (P < 0.01) and 26.7; 24.5; 7.3 g/d (P < 0.01) for LL, GS and CC groups, respectively. The conclusion is most of nitrogen from Calliandra was un-degraded and un-digested in the rumen and intestine thus excreted through feces, while most of the protein in Leucaena and Gliricidia was degraded in the rumen thus excreted through urine. Key Words: Leucaena, Gliricidia, Calliandra, Nitrogen-Kinetics, Urea Entry Rate

The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Ema Damayanti; Ahmad Sofyan; Hardi Julendra; Tri Untari
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 µL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum

Comparison of sequences of hypervariable region (HVR) subunit S-1 gene of field isolate I-37 infectious bronchitis virus with Connecticut serotype N.L.P Indi Dharmayanti; Risa Indriani; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 2 (2003): JUNE 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Infectious Bronchitis is a contagious and acute respiratory disease in chickens caused by infectious bronchitis virus (IBV).Antigenic differences in IBV are associated with changes in the sequence of the spike glycoprotein (S). The subunit S1 which demonstrates more sequence variability than S-2 have been identified as hypervariable region (HVR-1 and 2). There were several IB virus field isolates included I-37 have been identified in Indonesia by serum neutralization method. However, gene sequence variation in HVR subunit S-1 had not yet been identified. Isolate I-37 was close to the serotype Connecticut 46 (Conn 46). The aim of this study is to identify sequence variation of HVR subunit S-1 gene of isolate I-37 produced by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and sequencing. Several procedures were carried out in the study including virus titration, propagation and was concentrated from the allantoic fluid infected with IBV. Then, RNA was extracted for RTPCR. urther the product was sequnced and its homology with IBV references from GenBank was compared by GenMac version 8.0. Result showed that isolate I-37 produced 515 bp of amplification product. Isolate I-37 and Conn 46 are same serotype, yet their HVR subunit S-1 nucleotides and amino acids (protein) differ by 6.9% and 15.6% respectively. It might be concluded that isolate I-37 was variant of Conn 46. Key words: Sequences variation, IBV, I-37 field isolate, HVR subunit S-1 gene

The effect of sugar cane waste product fermented by Pleurotus ostreatus on the ration to Priangan sheep performance Ana Rochana Tarmidi
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Bagasse from Saccharum officinarum has potency as roughage source for ruminants. The objective of this research was to evaluate the use of bagasse fermented by Pleurotus ostreatus (ATB) to feed consumption, daily gain and feed efficiency of Priangan sheep. The research was arranged into two stages, i.e: (i) bioconversion of bagasse by P. ostreatus, and (ii) biological test using ATB. Twenty five heads of male Priangan sheep with average body weight of 18.99 + 0.22 kg were fed with ATB for three months. A completely randomized design was carried out with five replications. The treatment were: R0= 70.0% King grass + 30.0% concentrate; R1= 59.50% King grass + 10.10% ATB + 30% concentrate; R2= 49.00% King grass + 21.10% ATB + 30% concentrate; R3= 38.50% King grass + 31.50% ATB + 30% concentrates, and R4= 28.00% King grass + 42% ATB + 30% concentrate. The results of the experiment showed that bagasse fermented by P. ostreatus (ATB) did not indicate negative effects on feed consumption, daily gain, and feed efficiency of Priangan sheep, and ATB can be used as ingredient of Priangan sheep ration up to 31.50% based on dry matter of the ration. Key words: Bagasse, Pleurotus ostreatus, feed consumption, daily gain, feed efficiency

Molecular identification technique of Trypanosoma evansi by Multiplex Polymerase Chain Reaction Dyah H. Sawitri; April H. Wardhana; H. Wibowo; M. Sadikin; Fitrine Ekawasti
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Trypanosoma evansi is a Hemoflagella parasite that infects cattle and is known as the agents of Surra. Several other trypanosome species infects mammals: T. equiperdum, T. b. rhodesiense, T. b. gambiense, T. vivax, T. congolense, T.theileri. Some of these species is quite difficult to be distinguished morphologically with T. evansi through conventional techniques (thin blood smear). Molecular technique by polymerase chain reaction (PCR) is reported to have the ability to identify, characterize and diagnose trypanosomes accurately. However, a single PCR used is relatively expensive because it takes at least two or more pairs of primers to determine T. evansi. The purpose of this study is to develop T. evansi species identification techniques by multiplex PCR/mPCR (the three pairs of primer in one reaction) that takes the relatively fast and inexpensive. A total of 31 isolates T.evansi were obtained from Bblitvet Culture Collection (BCC) and the Department of Parasitology BBLitvet used in this study. Isolates represent isolates from endemic areas and Surra outbrake isolated from 1988-2014. DNA extraction performed on each sample, including Bang 87 isolates which has been purified as a positive control. Primers used are specific for T. evansi, the ITS-1, Ro Tat 1.2 VSG and ESAG 6/7. Before running mPCR, each primer is optimized by using a single PCR. The results showed that the three primers can be combined in a single reaction with mPCR technique and amplify each DNA fragment target perfectly, so identified 31 isolates as T. evansi. This technique can be applied in the field with a lower cost and faster time.Key Words: Trypanosoma evansi, Identification, Multiplex PCR

Forage and seed production of Puero (Pueraria javanica) in a Different Light intensity level Fanindi A; Sutedi E; Prawiradiputra BR
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 2 (2013): JUNE 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Puero (Pueraria javanica) is forage that can serve as a cover crop in plantations. The limiting factor for plant growth in the plantation is the light intensity, therefore the influence of light intensity on forage and seed production of Puero needs to be examined. Research was conducted at Kaum Pandak Research station of Indonesian Research Institute for Animal Production Bogor and Laboratory of Agrostology Faculty of Animal Husbandry, Bogor Agricultural University, for 16 months. Four levels of light intensity,i.e 100, 80,60 and 40% were applied, leguminosainous species Puero (Pueraria javanica), was used. The treatments were arangged in Randomized Complete Block Design with 3 replications. Data collected were analyzed by ANOVA and Duncan’s Multiple Range Test. Forage production was evaluated in one year. The forage quality and digestibility (invitro) were assessed. Seed production was recorded accumulatively from seasonal seed production during one year. Results show that light intensity affected (P < 0.05) forage and seed production, chlorophyll a and total chlorophyll of Puero, but did not affect (P > 0.05) quality and digestibility of Puero. The highest forage and seed production of Puero were obtained from full light intensity (100%). and seed production of Puero was affected (P < 0.05) by light intensity. The seed quality of Puero was also affected by light intensity. The best seed quality of Puero was achieved by from 80% light intensity. Key Words: Light Intensity, Forage Production, Seed, Puero

Antibody response in naïve and sensitised goats infested by Sarcoptes scabiei Simson Tarigan
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purpose of this study was to characterize the IgG and IgE antibody responses in goats infested repeatedly with Sarcoptes scabiei. Ten goats purchased from scabies-free farms were infested with 2000 live mites on the auricles. Fifty days after the initial infestation, the goats were treated with ivermectin. After being completely recovered, the goats were reinfested then treated again at 50 days post infestation. Blood samples were collected at the time of the first infestation, then every 10 days afterwards for 270 days. Seroconversion for IgG took place after 30 days following the first infestation, whereas the maximum level of the specific IgG antibodies occurred after 50 days. Immunoblot analysis identified a number of antigens (Mr 180, 135, 43 and 38 KDa) that recognised by the IgG at 10 days and continuously recognised throughout the course of the multiple infestations. Being consistently recognised, those antigens should be essential in the development immunological diagnostic tests for scabies. The levels of scabies-specific IgE antibodies increased slowly during the first infestation and rapidly dropped following treatment of the animals with ivermectin. In the second and third infestations, however, the reaginic antibodies rose rapidly and with a grater level. On immunoblot analysis, at least 10 antigens (Mr 130, 72, 64, 58, 48, 44, 41, 39, 27 and 25 KDa) were observed to be recognised by the IgE present in the sera from scabies-infested animals. Since IgE response is considered to play a major role in the immune protection, those allergens, therefore, could be used as the main component of an anti-scabies vaccine. Key words: Sarcoptes scabiei, antibody, goats

Morphological characteristics of forage crops indigofera zellongeriana at different levels drought stress and interval pruning Iwan Herdiawan; L Abdullah; D Sopandie; P.D.M.H Karti; N Hidayati
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 4 (2012): DECEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The objectives of this experiment was to evaluate the effect of drought stress and defoliation interval on shoot and root morphologic characteristic of Indigofera zollingeriana. The experiment was arranged in a completely randomized design with two factors and four replications. The first factor consisted of 3 level of drought stress i.e: 100% field capacity (FC) (control), 50% FC, and 25% FC. The second factor comprised of 3 defoliation interval were interval defoliations of 60, 90 and 120 days. The observed variables were shoot, root dry weight, root/shoot ratio and root length. Data were analyzed by ANOVA and the differences between treatments were tested by LSD. The results shows that there were interactions (P < 0,05) between drought stress and defoliations interval on shoot dry weight, while root dry weight, root/shoot ratio, and root length was not. Drought treatment significantly (P < 0,05) decreased shoot, root dry weight, but increase of root/shoot ratio and root length. Defoliation interval significantly affected (P < 0,05) on shoot dry weight, but not on root dry weight, root/shoot ratio, and root length. Key Words: Indigofera zollingeriana, Drought Stress, Defoliation Interval, Shoot And Root Morphology

In vitro slow-release urea characteristics under different molasses levels contained in rice straw based diets D. Kardaya; K.G. Wiryawan; A. Parakkasi; H.M. Winugroho
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Slow-release urea characteristics of zinc-urea, zeolites-urea, and zeolites-zinc-urea were examined using in vitro techniques. The objective of this experiment was to study the in vitro slow-release urea characteristics of zinc-urea, zeolites-urea, and zeolites-zinc-urea under different molasses concentrations in relation to the ruminal fermentative changes observed in different incubation time. The experimental design employed was randomized block design with a 4 x 3 factorial arrangement plus a control treatment, and conducted in two replications. Factors were various urea sources (urea, zinc-urea, zeolites-urea, and zeolites-zinc-urea) and molasses concentrations (0%, 6%, and 12%) in rice straw based diets. The control treatment was rice straw based diet containing neither urea nor molasses. Diets consisted of 45% rice straw and 55% concentrates (DM basis) were formulated to have similar N and TDN levels. Responses of parameters measured were subjected to MANOVA using the GLM procedure of SPSS 16.00 and differences among mean values, if applicable, were examined using HSD-test. Orthogonal comparisons were used to determine the effects of control treatment vs. various urea sources following significance for the two-factor ANOVA model. Results indicated that zinc-urea, zeolites-urea, and zeolites-zinc-urea under different molasses concentrations contained in rice straw based diets decreased ruminal ammonia up to 48 hours incubation, controlled total VFA level and pH values revealed from lower NH3:VFA ratio, and improved both in vitro dry matter and organic matter degradabilities. The best impact of the in vitro slow-release urea characteristics of zinc-urea, zeolites-urea, and zeolites-zinc-urea on the ruminal fermentative changes (NH3, VFA, pH, DMD, OMD) was well attributed to the diets contained 6% molasses. Key words: Slow-Release Urea, Molasses, Rice Straw, In Vitro

Page 146 of 176 | Total Record : 1756

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Contact Name Ahmadi Riyanto

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Location Kota bogor, Jawa barat INDONESIA

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
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Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA