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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 202 Documents

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Analisis Restorasi Ekosistem Wilayah Pesisir Terpadu Berbasis Co-Management: Studi Kasus Di Kecamatan Ujung Pangkah Dan Kecamatan Bungah, Kabupaten Gresik Rudianto Rudianto
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Penelitian ini dilakukan dengan fokus melakukan restorasi ekosistem wilayah pesisir dengan pendekatan keterpaduan di Kabupaten Gesik dengan mengambil contoh di dua kecamatan yaitu Kecamatan Ujung pangkah dan Kecamatan Bungah. Restorasi merupakan upaya untuk memperbaiki kembali kondisi wilayah pesisir yang sudah rusak lingkungannya akibat dari kegiatan manusia yang makin tidak rasional dan karena proses alam yang perubahannya sangat dtrastis akibat pengaruh global warming. Restorasi berupaya untuk melakukan perbaikan untuk mengembalikannya ke kondisi semula, walaupun untuk mencapai pemulihan kepada kondisi semula masih memerlukan penelitian lebih lanjut. Hal ini mengingat kondisi yang mendukung seperti kondisi hidrologi sudah mengalami perubahan.Metode yang digunakan untuk mencapai tujuan penelitian ini digunakan pendekatan kombinasi yaitu penelitian diskriptif, penelitian partisipatif dan penelitian eksploratif. Penelitian diskriptif terkait dengan observasi dilapang untuk menilai kondisi kedua kabupaten baik secara biogeofisik, sosial ekonomi dan sosial budaya. Untuk penelitian partisipatif berkaitan dengan menjaring aspirasi dan harapan masyarakat melalui wawancara. Sedangkan penelitian eksploratif digunakan model Partial Least Square (PLS) dan Analitycal Hierarchy Process (AHP). Disamping itu untuk merumuskan model restorasi ekosistem terkait pengelolaan digunakan model co-management.Hasil penelitian menunjukkan bahwa hubungan antara masyarakat dengan upaya restorasi ekosistem ternyata sangat kuat berperan, termasuk upaya restorasi perlu dilakukan secara terpadu. Hal ini ditunjukkan oleh hasil PLS. Sedangkan ekosistem pesisir mana yang harus didahulukan untuk ditangani AHP menyatakan bahwa prioritas utama adalah mangrove, kemudian terumbu karang, diikuti dengan kawasan estuari dan terakhir padang lamun. Berdasarkan itu maka model co-management mensyaratkan perlu adanya kolaborasi diantara masyarakat, pemerintah dan swasta. Kolaborasi tersebut dipayungi oleh kerangka strategi yang memuat visi, misi, tujuan dan prioritas strategi.

Pengaruh Waktu Panen Terhadap Kandungan Glukomannan Pada Umbi Porang (amorphophallus muelleri blume) Periode Tumbuh Ketiga Nurul Chairiyah; Nunung Harijati; Retno Mastuti
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Waktu panen diduga berpengaruh terhadap kandungan glukomannan pada umbi porang. Penelitian ini bertujuan untuk mengetahui pengaruh waktu panen terhadap dinamika kandungan glukomannan pada umbi porang. Glukomannan diekstraksi dari umbi porang yang diperoleh pada saat dua minggu sebelum tanaman rebah, pada saat tanaman rebah dan dua minggu setelah tanaman rebah. Glukomannan diperoleh dengan menggunakan metode ekstraksi yang telah dimodifikasi. Parameter yang diamati meliputi kandungan glukomannan dan berat kering umbi. Data kandungan glukomannan pada umbi dianalisis dengan One way ANOVA dan dilanjutkan dengan Tukey α 0,05. Sedangkan hubungan antara kandungan glukomannan terhadap berat umbi dianalisis dengan menggunakan Uji Korelasi Bivariate. Dari hasil analisis, Kadar glukomannan cenderung lebih tinggi pada umbi porang yang dipanen saat tanaman rebah dibandingkan dengan glukomannan pada umbi yang diperoleh ketika sebelum dan setelah tanaman rebah yaitu sebesar 29,10 ± 4,57 g/100 g. Peningkatan kandungan glukomannan dapat meningkatkan berat umbi porang.

The Effect of Lactobacillus Acidophilus Microcapsule Which Encapsulated by Kappa Caragenan Toward in Vivo Functional Test Dwi Setijawati; Muhammad Firdaus; Kartikaningsih Kartikaningsih
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The effect of Lactobacillus acidophilus microcapsule which encapsulated by kappa-caragenan toward in vivo functional test has been carried out. The goal were to get the viability of L. acidophilus and E. coli in the stool test animals which fed by L. acidophilus encapsulated kappa-caragenan, to get anti-cholesterol ability in feces test animals which fed by L. acidophilus encapsulated kappa- caragenan, gain the ability to decrease the activity of b - glukoronidase found in feces test animals by administration of L. acidophilus which encapsulated by kappa-carrageenan, get the ability of L. Acidophilus which encapsulated by kappa-carrageenan as an inducer of IgA obtained from stool test animals. Conclusion were the administration L. acidophilus encapsulated by kappa- caragenan may improve the viability of L. acidophilus and E. coli feces of mice and can raise cholesterol levels feces of mice and decrease the activity of b - glukoronidase feces of mice and increase levels of fecal IgA mice.

Engineering Technology for Increasing Soybean Production and to Control Damping-off Disease Using Biological Fertilization Mixture Ika Rochdjatun Sastrahidayat
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Fall seedling diseases (damping-off) caused by Sclerotium rolfsii is one factor in low productivity of soybean in Indonesia, which may cause loss results achieved 30-75% depending on the level of attacks. Given the character of soil borne diseases (soil borne), then all control efforts through many obstacles, either because the pathogen can survive for decades in the form resistant propagules (Sclerotium) although there is no host for the development.This study aimed to find ways to control the disease by utilizing the potential of natural biological materials and find ways to get control for effective results. For this purpose the various steps of research has been conducted both in laboratory and greenhouse using experimental research design (completely randomized design) and F test to see the difference. The results show that in the first year of research proven to provide results tuba root extract is effective in controlling pathogens. Streptomyces isolates proved to be antagonistic to Sclerotium rolfsii, and when combined with mycorrhizae (AM) will be able to increase its effectiveness. Introduction of inoculum will likely increase their effectiveness when mixed directly into cropping land that can be done as a biological fertilizer just before planting. There is a tendency that the combination treatment between biopesticide will help the effectiveness of its control, though not necessarily affect the production, so the increase in soybean production is due to the protection of soybean from the dead early in the seedling that can suppress up to 50 percent compared with the control.

Estimation of Diverse Porang (Amorphophallus Muelleri Blume) Age in Forest Are Based on Brancing Pattern of Leaf Petiolule Harijati, Nunung; Mastuti, Retno
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Porang is higher plant which has unique morphology. Tuber, petiole and lamina are the main part of its body. Even Porang doesn’t have true stem, only petiole, its lamina not simple leaves but compound leaves with special pattern. Branching patterns of petiolule are not same in different age. Therefore the aim of research was to observe branching pattern of petiolule from Porang age 1-4 that lived in their native habitat i.e. forest. The research was conducted in secondary forest in Sumberbendo village, Madiun. Determination porang age was helped by expert farmer that worked with porang for long time. The result showed that Porang age 1 had petiolule with braching type 1-0. Porang age 2 was 1-3, Porang age 3 was 1-3-2, and Porang age 4 was 1-3-2-2. The petiolule which acted as a base of new branch had one or few single simple leaf. The leave could be both sinus and un-sinus leaves located in between two initial or base branching. The position of the leaves were opposite or alternate along with petiolule. If location single leaf just in point branching, the new petiolule morphology was not be considered as petiolule but midrib.

Using Wheat Flour and Alginate In Mozzarella Cheese Making On Physical and Sensory Quality Purwadi Purwadi; Abdul Manab
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The purpose of this research was to know the best combination of using wheat flour and alginate on physical and sensory quality of Mozzarella cheese.The method that used in this research was factorial experiment with completely randomized design by using twelve treatments and three times repeatation. The variable measured was hardness, cutting point and sensory. The obtained data was analized by using analysis of variance continued by honesty significant difference (HSD).The result of this research showed that the used of wheat flour and alginate did not give a significance different interaction (P > 0,05) on the average of hardness, cutting point and sensory quality (colour, texture, and taste) of Mozzarella cheese, but used of wheat flour give a different significance effect (P < 0,05) on hardness and cutting point of Mozzarella cheese. The highest value was the treatment of T4A3, that was the combination of 7,5 % wheat flour and 1 % alginate.

α-Amylase and α-Glucosidase Inhibition by Brown Seaweed (Sargassum sp) Extracts Muhammad Firdaus; Asep Awaludin Prihanto
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Inhibition of intestinal α-amylase and α-glucosidase is an important strategy to control post-prandial hyperglycemia associated with diabetes mellitus. In vitro inhibitory effects of crude extracts of seaweed against α-amylase and α-glucosidase were studied. Crude ethyl acetate extracts of Sargassum aquifolium were the stronger inhibitor to α-amylase and α-glucosidase than others. Furthermore, Sargassum aquifolium ethyl acetate extract significantly suppressed the rise in postprandial glucose level after oral administration of glucose in normal rats. The results of this study suggest that the crude Sargassum aquifolium extract may suppress the rise in postprandial hyperglycemia in vivo in part, through inhibition of alpha amylase and glucosidase.

Marker Spesifik Combine DNA Index System (CODIS) 13 dalam Identifikasi Forensik pada Suku Jawa dan Madura di Indonesia Wening Prastowo; Fitria Dewi Listiani
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Forensic identification can be performed DNA examination. FBI had recommended, forensic DNA examination to used short tandem repeat CODIS 13. Result of determine Short tandem repeat CODIS 13, showed specific pattern. Java tribes are shown, the Heterozygousity Index ranged from 0,60069 (VWA) to 0.93752 (D18S51), the Power of Exclution ranged from 0.30885 (VWA), to 0.83068 (D18S51), the Power of Discrimination ranged from 0.52853 (VWA), to 0.99127 (D18S51). Madura tribes shown, the Heterozygousity Index ranged from 0,63021 (VWA) to 0.94445 (FGA), the Power of Exclution ranged from 0.33418 (VWA), to 0.84779 (FGA), the Power of Discrimination ranged from 0.60501 (TPOX), to 0.99305 ( FGA).

Effect of Noni Leaves Extract (Morinda citrifolia L.) Supplementation in Feed on Physical Quality of Broiler Breast Meat Aji Sukoco; Eko Widodo; Imam Thohari
Research Journal of Life Science Vol 2, No 2 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This research was conducted to know the effect of noni leaves extract (Morinda citrifolia L.) supplementation in feed on physical quality of broiler breast meat such as pH, Water Holding Capacity (WHC), Cooking Loss (CL), and tenderness. Ninety six 8-days old broiler chickens strain Lohmann and of undifferentiated sex (unsexed) were used in this research. The broiler chickens will be reared until 35-days old. The research method was experimental using Completely Randomized Design (CRD) with six treatments and four replications, each replication consisted of four broiler chickens. The treatments consisted of P0 (Basal Feed), P1 (Basal Feed + tetracycline 0.05%), P2 (Basal Feed + noni leaves extract 0.05%), P3 (Basal feed + noni leaves extract 0.1%), P4 (Basal feed + noni leaves extract 0.15%), P5 (Basal feed + noni leaves extract 0.2%). The data were analyzed by ANOVA and continued by Least Significant Difference (LSD) test if there was significantly different result. The results showed that noni leaves extract did not give significant effect (P>0.05) on meat pH, water holding capacity (WHC), cooking loss (CL), and tenderness. However, these results were still acceptable normally such as pH between 5.38-5.57, water holding capacity 34.13-45.64%, cooking loss 33.05-36.97%, but tenderness 16.22-20.57N were less acceptable. The research concluded that supplementation of noni leaves extract (Morinda citrifolia L.) in feed did not increase physical quality of broiler breast meat on pH, Water Holding Capacity (WHC), Cooking Loss (CL), and tenderness.

Effect Of Fish Oil Alone or In Combination With Tomato Powder Supplementation In Feed On Egg Quality of Local Ducks Faizal Andri; Eko Widodo; Osfar Sjofjan
Research Journal of Life Science Vol 2, No 2 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The purpose of this research was to investigate the effect of fish oil alone or in combination with tomato powder supplementation in feed on egg quality of local ducks. Fivety 28-weeks old female local ducks with initial egg production of 4 days before research was 55.00 + 4.08% (coefficient of variation 7.42%) were randomly distributed to five treatments with 2 repetition and 5 birds of each. The treatmens were T0: basal feed (control); T1: basal feed + 1500 ppm fish oil ; T2: basal feed + 3000 ppm fish oil; T3: basal feed + 1500 ppm fish oil + 150 ppm tomato powder; T4: basal feed + 3000 ppm fish oil + 150 ppm tomato powder. Variable observed in this research were egg shape index, egg shell weight, yolk weight, and albumen weight. Data were analyzed using one-way Anova based on Completely Randomized Design, if significant effect appear was then continued with Duncan Multiple Range Test. The result showed that there were no significant effect (P>0.05) of fish oil alone or in combination with tomato powder supplementation in feed on egg shape index, egg shell weight, yolk weight, and albumen weight of local ducks. The conclusion of this research is that there was no effect of fish oil alone or in combination with tomato powder supplementation in feed on egg quality of local ducks.

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Phone +62341 575825

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Kuswanto

Contact Email
rjls@ub.ac.id

Phone
+62341 575825

Journal Mail Official
rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA