cover
Contact Name
Masruri
Contact Email
masruri@ub.ac.id
Phone
+62341-575838
Journal Mail Official
jpacr@ub.ac.id
Editorial Address
Departemen Kimia, Fakultas MIPA, Universitas Brawijaya, Jl. Veteran 65145 Malang
Location
Kota malang,
Jawa timur
INDONESIA
The Journal of Pure and Applied Chemistry Research
Published by Universitas Brawijaya
ISSN : 23024690     EISSN : 25410733     DOI : http://dx.doi.org/10.21776/ub.jpacr
The Journal of Pure and Applied Chemistry Research focuses in publishing research articles in the field of Chemistry and Applied Chemistry. The target is in exploring, investigating, and developing chemicals sources from local and/or Indonesian to increase the value. Scope of the journal is organic chemistry, analytical chemistry, inorganic chemistry, biochemistry, and physical chemistry. Included the topic also organic chemistry natural product, theoretical and computational chemistry.
Articles 7 Documents
Search results for , issue "Vol 2, No 2 (2013)" : 7 Documents clear
Study on iNOS expression and levels of Malondialdehyde in the White Rat (Rattus norvegicus) Exposed with Aggregatibacter actinomycetemcomitans Reviana Setin; Aulanni'am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.592 KB) | DOI: 10.21776/ub.jpacr.2013.002.02.125

Abstract

Aggregatibacter actinomycetemcomitant (Aa) is gram-negative bacteria composed of lipopolysaccharides (LPS) in its outer membrane. This bacteria is normally found in dental plaque and periodontal pockets causing parotid gland damage when it is present in excess amounts. LPS is an endotoxin and a complex glycolipid composed of polysaccharides (hydrophilic) and lipid A (hydrophobic) which can induces Reactive Oxygen Species (ROS), and increase the levels of malonaldialdehyde (MDA) and tissue damage. The aims of this research are to find the expression of iNOS parotid gland cell and to determine levels of MDA using thiobarbituric acid (TBA) spectrophotometric test at 533 nm. The results showed a significantly difference (P<0.05) of MDA levels in control rats (3.135 ± 0.0436 ppm) and Aa rats (6.307 ± 0.0473 ppm). iNOS expression is confirmed by the brown spots formed on the parotid gland, affording the enhancements of iNOS expression in Aa rats and control rats of 2.292 ± 0.038 and 0.166 ± 0.003, respectively (P<0.05). Keywords       : Aggregatibacter actinomycetemcomitans, Reactive Oxygen Species, malondialdehyde, iNOS
Analysis Proton Conducting Electrolyte IT-SOFC Hybrid System Exhaust Gas With External Reforming of Biofuel Nizar Amir; I.N.G. Wardana
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.555 KB) | DOI: 10.21776/ub.jpacr.2013.002.02.134

Abstract

In this analysis, a hybrid system containing proton SOFC (P-SOFC) combine with micro gas turbine (MGT) with biofuel external reforming is investigation to decrease the greenhouse gases problem facing in electrical power plant. The hybrid system consist of a proton solid oxide fuel cell stack, a micro gas turbine, a combustor, compressors, heat exchangers and external reformer. The main operating parameter such as, fuel utilization and steam - carbon ratio is determined in this analysis.
Effect of D-Alpha Tocopherol Therapy towards Malondialdehyde Level and Histology of Kidney in Rattus norvegicus with MLD-STZ Induction Marissa Agnestiansyah Mahardhian; Aulanni’am Aulanni&#039;am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (689.86 KB) | DOI: 10.21776/ub.jpacr.2013.002.02.137

Abstract

Diabetic Nephropathy is a kidney disease which occurs due to complication of diabetes mellitus  as a consequence of the damage of the kidney endothelial cells. Hyperglicemia condition in patients with diabetes mellitus that induces an oxidative stress, were related to endothelial cell damage. Oxidative stress as a result of hyperglycemia will activate a number of signal transduction pathways resulting in increase of  free radicals. D-alpha tocopherol as one of antioxidant substance, that can act as an inhibitor of free radical chain reactions, play an important role in the reduction of the oxidative stress effect. Effect of D-alpha-tocopherol in reducing oxidative stress is identified by the levels of malondialdehyde (MDA) in kidney and histology of kidney. This study used five groups mice; they were a control group, a diabetic group which was induced with MLD-STZ, and a therapeutic groups with a varieties doses of D-alpha tocopherol (100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW). The results showed that the D-alpha tocopherol was able to reduce the levels of malondialdehyde (MDA) and repair the histology of kidney of mice induced by MLD-STZ. 
Effect of D-Alpha Tocopherol Therapy towards Malondialdehyde Level and Histology of Kidney in Rattus norvegicus with MLD-STZ Induction Agnestiansyah, Marissa; Mahdi, Chanif
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.207 KB)

Abstract

ABSTRACT Diabetic Nephropathy is a kidney disease which occurs due to complication of diabetes mellitus  as a consequence of the damage of the kidney endothelial cells. Hyperglicemia condition in patients with diabetes mellitus that induces an oxidative stress, were related to endothelial cell damage. Oxidative stress as a result of hyperglycemia will activate a number of signal transduction pathways resulting in increase of  free radicals. D-alpha tocopherol as one of antioxidant substance, that can act as an inhibitor of free radical chain reactions, play an important role in the reduction of the oxidative stress effect. Effect of D-alpha-tocopherol in reducing oxidative stress is identified by the levels of malondialdehyde (MDA) in kidney and histology of kidney. This study used five groups mice; they were a control group, a diabetic group which was induced with MLD-STZ, and a therapeutic groups with a varieties doses of D-alpha tocopherol (100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW). The results showed that the D-alpha tocopherol was able to reduce the levels of malondialdehyde (MDA) and repair the histology of kidney of mice induced by MLD-STZ.   Keywords: Diabetic Nephropathy, Diabetes mellitus, MLD-STZ, Malondialdehyde, D-Alpha tocopherol  
Adsorption of Cadmium By Silica Chitosan Moftah Ali; Ani Mulyasuryani; Akhmad Sabarudin
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.102 KB) | DOI: 10.21776/ub.jpacr.2013.002.02.141

Abstract

The adsorption process depends on initial concentration of Cd2+ and ratio of  chitosan in adsorbent. The present study deals with the competitive adsorption of Cd2+ ion onto silica graft with chitosan. Batch adsorption experiments were performed at five different initial Cd2+ concentrations (5, 10, 15, 20 and 25 ppm), on five different proportion from silica to chitosan (100%, 95%, 85%, 75% and 65%) as adsorbent at pH 5. In the recovery process, the high recovery at 0.5 mg and observed the recovery decrease with increasing the initial concentration of Cd2+, and the low recovery at 0.25 mg from Cd2+. In this study, the adsorption capacity of Cd2+ in regard to the ratio of silica and chitosan hybrid adsorbents are examined in detail. The aim of this study to explore effects of initial concentrations of Cd2+, and the ratio of silica to chitosan on the adsorption and recovery of Cd2+.
Carbon Nanotube Covalently Attached Laccase Biocathode for Biofuel Cell Rizmahardian Ashari Kurniawan; Aulani Aulanni&#039;am; Fa-Kuen Shieh; Peter Po-Jhen Chu
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (843.173 KB) | DOI: 10.21776/ub.jpacr.2013.002.02.143

Abstract

Biocathode for biofuel cell was prepared by covalently immobilizedLaccaseon CNT (CNT-Laccase) using glutaraldehyde as conjugates. Successful laccase immobilization was confirmed by Fourier Transform Infrared (FTIR) Spectrophotometry, Surface Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). Immobilization affected Laccase enzymatic activity where it boosts the stability at high temperature and neutral pH. At temperature 65ºC, free Laccase completely loss its activity, while CNT-Laccase still retaining 57.12% of its activity at 45ºC. The activity of CNT-Laccase at pH 7 was 7.04% of activity at pH 5 which was higher than that of free Laccase. CNT-Laccase was able to perform oxygen electroreduction with addition ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as mediator. Performance of oxygen electroreduction activity was also determined by type and composition of binding polymer. Nafion was able to provide better environment for oxygen electroreduction activity compare to polyvinyl alcohol (PVA). Current density resulted in using Nafion in ratio 1:10 to buffer volume was 1.31 mA/cm2, which was higher than that of PVA (1.01 mA/cm2). Increasing binding polymer ratio into 1:2 and 1:1 undermined oxygen electroreduction activity.
The Influence of Conditioning Agent on Phosphate Diffusion Coefficient through Polyacrylamide and Agarose Gel Layta Dinira; Barlah Rumhayati; Rachmat Triandi Tjahjanto
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.522 KB) | DOI: 10.21776/ub.jpacr.2013.002.02.155

Abstract

Excess phosphate in natural water can cause algae grow rapidly, to the extent causing many fish deaths that led to the extinction of certain species. Therefore, an analysis or periodic observations of phosphate levels in the water is needed. The commonly used method is diffusive gradient in thin films (DGT) technique. The DGT technique is based on the ability of analyte to diffuse through a gel, which have a value named diffusion coefficient. This research was conducted in order to study the effect of different storage solution to the phosphate diffusion coefficient through polyacrylamide and agarose gels. Initial research performed with making the polyacrylamide and agarose gels. To observe the effect of different storage solutions, the gels partly stored in distilled water gel while the others are stored in a NaCl solution of 0.01 M. Phosphate diffusion coefficient was determined using Fick's Law after analyze the phosphate concentration using UV-Visible spectrophotometer. The results showed that phosphate diffusion coefficient was highest when polyacrylamide and agarose gels stored in NaCl solution of 0.01 M.

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