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AGRIVITA, Journal of Agricultural Science
Published by Universitas Brawijaya
ISSN : 01260537     EISSN : 24778516     DOI : https://doi.org/10.17503
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research papers and article review in plant science i.e. agronomy, horticulture, plant breeding, soil sciences, plant protection and other pertinent field related to plant production. AGRIVITA is published three times per year. The Journal has been indexed in SCOPUS, Scimago Journal Ranks (SJR), Emerging Source Citation Index ( ESCI-Web of Science), EBSCO, ProQuest, Google Scholar and others international indexing. AGRIVITA is accredited first grade (Sinta 1/S1) for five years (2018-2023) based on Decree No: 30/E/KPT/2018 by Ministry of Research, Technology and Higher Education (Ristek Dikti), The Republic of Indonesia. We accept submission from all over the world. All submitted articles shall never been published elsewhere, original and not under consideration for other publication.
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Articles 934 Documents
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Efficient Strategies for Elimination of Phenolic Compounds During DNA Extraction from Roots of Pistacia vera L. Saeideh Rajaei; Rana Sabagh Farshi; Maryam Moazzam Jazi; Seyed Mahdi Seyedi
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.734

Abstract

Optimization of DNA extraction protocols for plant tissues and including endophytic microorganisms is a critical step of advanced plant-microbe interaction in agricultural studies. Pistachio (Pistacia vera L.) root tissue contains high levels of polyphenols have been known as major extract contaminants and inhibitors of enzymatic activities during amplification. The present study aimed to develop reliable strategies to purify DNA from Pistachio root samples. Inhibiting substances were removed from DNA through a process including extraction with hot detergent contains SDS-Tris- EDTA, AlNH4(SO4)2.12H2O as chemical coagulating factor and CTAB-NaCl. Following typically organic extraction/alcohol precipitation, denaturing agarose electrophoresis performed to purify probable remain contaminants. The purified DNA was enough free of polyphenols based upon loss of color and spectral quality (260/230>1.6) and efficiently amplified during polymerase chain reaction particularly in the present of GC-clamp primers. This method proved well with detection of Glomus sp. (arbuscular mycorrhiza fungi) associated with Pistacia vera L. using denaturing gradient gel electrophoresis (DGGE).
Does Landscape Complexity and Semi-Natural Habitat Structure Affect Diversity of Flower-Visiting Insects in Cucumber Fields? Akhmad Rizali; Damayanti Buchori; Susilawati Susilawati; Pudjianto Pudjianto; Yann Clough
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.1722

Abstract

Presence of insects in agricultural habitat is affected by the surrounding circumstances such as the complexity and structure of landscape. Landscape structure is often formed as a consequence of the fragmentation of semi-natural habitat, which can negatively affect species richness and abundance of insects. This study was aimed to study the effect of complexity and structure of landscape on the diversity, abundance and traits of flower-visiting insects in cucumber fields. This study was conducted in cucumber fields surrounded by other agricultural crops, shrubs, semi-natural habitat and housing area, in Bogor, Cianjur and Sukabumi regencies, West Java, Indonesia. In a total of 16 agricultural areas, complexity and parameter of landscape especially class area (CA), number of patches (NumP), mean patch size (MPS), total edge (TE), and mean shape index (MSI) of seminatural habitats were measured. Sampling of flower-visiting insects was conducted using scan sampling methods. The result showed that landscape complexity affected species richness (but not abundance and trait) of flower-visiting insects both for mobile and less-mobile insects. Flower-visiting insects also responded differently to landscape structure. Species richness, abundance and variation of body size of mobile insects were affected by structure of semi-natural habitat.
Production Enhancement Technology of Pegagan (Centella asiatica) Noverita Sprinse Vinolina; Marline Nainggolan; Rolan Siregar
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.469

Abstract

Centella asiatica has long been known as one of medicinal plants. Profound study is needed to be able to find out the responses of Pegagan to various treatments in order to increase centelloside content. It was also previously reported that the highest asiaticoside content was found at the lowland pegagan, Pantai Labu. Current study on planted lowland pegagan of Deli Serdang with 2.38% of asiaticoside content was aimed to know the response of the plants to phosphorus fertilizing, elicitorand harvesting time related to biomass production from leaves, petioles, roots and tendrils. Phosphorus fertilizer was applied in four levels: 0, 18, 36 and 54 kg P2O5.ha-1, which affected centelloside synthesis, and elicitor of methyl jasmonate was given at three levels: 0, 100, 200 μM, while harvesting time was set up at 56, 70 and 84 days after planting (DAP). Data showed that the best combination for biomass production was obtained when there was no elicitor and harvesting was done at 84 DAP. The best combination between methyl jasmonate hormone and harvesting period in both fresh weight of leaves and petioles as well as roots was obtained when there was no hormone  and harvesting time was 84 days old.
PLANT SPECIES DIVERSITY IN RELATION TO CARBON STOCKS AT JANGKOK WATERSHED, LOMBOK ISLAND Markum, Markum; Soesilaningsih, Endang Arie; Suprayogo, Didik; Hairiah, Kurniatun
AGRIVITA, Journal of Agricultural Science Vol 35, No 3 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i3.315

Abstract

ABSTRACTThe area around Jangkok watershed has changed, dividing it into some land use systems. This research aimed to study the effect of plant species diversity onC-stocks, and to analyse the characteristics of land use systems based on their similarity. The observations were carried out on 18 plots representing six land use systems in Jangkok watershed (Lombok Island) i.e. primary forest (PF), disturbed forest (DF), Mahogany- woodlot (MW), candlenut- agroforestry (CA), multistrata- agroforestry (MA), and simple- agroforestry (SA).The species diversity level was measured using Shannon-Weiner diversity index, whileC-stocks according to the method of RaCSA (Rapid Carbon Stock Appraisal). Results showed that forest conversion to agricultural land usesreduced the number and density of the species, average wooddensity andtree basal area. The PF represented the highest biodiversity index (3.46), while the other land uses were categorised in medium and low. Largest C-stock wasfound inPF and in(30 years old) of MWaveraged of 500 Mg ha-1, while the lowest was in SAof 68 Mg ha-1, while in other land uses was 219 Mg ha-1.The quantity of C-stock was not related tospecies diversity and its density, but closely related (pof tree (R2=0.84), basal area of all sizes tree (R2=0.86), and with the basal area of big trees (diameter > 30 cm) (R2=0.71). Based on the number and species density, agroforestry system (MA and CA) resembled the characteristics of natural forest (DF and PF)./spanEN-GBspan style=
The Endophytic Bacteria, Salicylic Acid, and their Combination as Inducers of Rice Resistance Against Xanthomonas oryzae pv. oryzae Christoffol Leiwakabessy; Meity Suradji Sinaga; Kikin H. Mutaqien; Trikoesoemaningtyas Trikoesoemaningtyas; Giyanto Giyanto
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.1029

Abstract

Bacterial leaf damage or blight brought by bacteria Xanthomonas oryzae pv. oryzae (X. oryzae pv. oryzae) is considered as an extremely serious disease of rice worldwide, including Indonesia. Induced resistance using chemical and biological agents was considered as a method to control the disease. The objectives of this research were to analyze of endophytic bacteria (Lysinibacillus sphaericus/L.sphaericus) and salicylic acid as the inducers of rice resistance against X. oryzae pv. oryzae. This study used three-factorial incompletely randomized designs to study the effect of endophytic bacteria and salicylic acid on three varieties of rice. The results of the study showed that endophytic bacteria and salicylic acid induced rice resistance to X. oryzae pv. oryzae. This endophytic bacterium was also able to increase the plant height (AUPHGC), the number of tillers (AUNTGC), and the number of productive tillers. The application of salicylic acid at the concentrations of 10 mM was able to suppress the BLB disease and increased the number of tillers and 1000-grain weight. The result of study showed that the endophytic bacteria and salicylic acid could induce resistance of rice varities against BLB disease and increased the number of productive tillers.
Identification of Quorum Quenching Bacteria and Its Biocontrol Potential Against Soft Rot Disease Bacteria, Dickeya dadantii Syaiful Khoiri; Tri Asmira Damayanti; Giyanto Giyanto
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i1.633

Abstract

Dickeya dadantii is one of newly found bacteria causing soft rot on orchids in Indonesia. Infected plants showed severe rot rapidly only in few days. An effort to control the bacteria was conducted by utilizing selected quorum quenching (QQ) inducer bacteria which produce AHL-lactonase by aiiA gene. The aims of this research were to screen and identify of quorum quenching bacteria, and also assayed their biocontrol potential ability against D. dadantii in laboratory. The screening of QQ bacteria was achieved using the anti-QS test, anti-microbial activity, and detection of aiiA gene using specific primer. The determination of the ability against D. dadantii was done using the soft rot assay on potato and orchid. Among thirty one bacteria isolates screened, four isolates (in succession namely B37, BT2, GG3, and GG6) were selected to control D. dadantii. All of these bacteria showed QQ ability to suppress the virulence of D. dadantii infection on orchids, significantly. Based on nucleotide sequences of 16S ribosomal RNA, those of bacteria isolates had the highest identity with Brevibacillus brevis, Bacillus cereus ATCC14579, Bacillus cereus ATCC14579 and Bacillus thuringiensis ATCC 10792. Brevibacillus brevis was reported for the first time as QQ bacteria in this study.
A Study of the Efficacy of Various Nutrient Sources on the Growth and Yield of Cabbage Maghfoer, Moch. Dawam; Koesriharti, Koesriharti; Islami, Titiek; Kanwal, N. D. S.
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.1721

Abstract

Research evaluated various nitrogen sources, and a supplementary foliar fertilizer or biochar on cabbage. It was conducted from July to October 2014 in Poncokusumo, Malang ca. 600 meters above sea level. The experiment used a randomized block design with four replications. The treatments were fertilized with different concentrations of urea, goat manure and selected substances i.e. 100% N urea (controls); 75% N urea + 25% N goat manure; 50% N urea + 50% N goat manure; 75% N urea + 25% N goat manure + foliar fertilizer (6-30-30), 50% N urea + 50% N goat manure + foliar fertilizer (6-30-30); 75% N urea + 25% N manure + biochar; and 50% N urea + 50% N goat manure + biochar. The dose of N fertilizer used was 69 kg ha-1. The results showed that a higher yield was recorded in the application of 75% N urea + 25% N goat manure and 50% N urea + 50% N manure with an addition of foliar fertilizer that was not significant with the application of 75% N urea + 25% N goat manure with the addition of biochar, resulting in a marketable yield of 68.84, 66.5 and 64.75 t ha-1, respectively.
Biological Control of Brown Leaf Spot Disease Caused by Curvularia lunata and Field Application Method on Rice Variety IR66 in Cambodia Huyly Tann; Kasem Soytong
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i1.768

Abstract

Curvularia lunata was found to cause a serious rice brown leaf spot in Cambodia. This is the first report of brown leaf spot on rice in Cambodia. All isolates were tested for pathogenicity. Dual culture antagonistic tests showed that Chaetomium cupreum inhibited sporulation of C. lunata when compared to the control. In a pot experiment, C.  cupreum significantly reduced the incidence of brown leaf spot caused by C. lunata. After application of a spore suspension of C. cupreum, Chaetomium-biofungicide and chemical fungicide (tebuconazole) to rice seedlings inoculated with C. lunata, the disease was reduced by 68.79 %, 75.80 % and 72.41 %, respectively. In a field trial, the chemical method gave the best results in all plant parameters, followed by the good agricultural practice (GAP) and organic methods. The chemical method gave the highest panicle/plant, panicle length, panicle weight, grain weight/plant which were different from the GAP and organic methods. The chemical method also gave the best results in filled grain panicle-1, unfilled grain panicle-1, grain weight plot-1, dry hay weight plot-1, biomass weight plot-1 and harvest index, and was significantly better than the GAP and organic methods.
Effects of Sugar Type and Concentration on Batu 55 Mandarin (Citrus reticulata Blanco.) Somatic Embryo Maturation Wahyu Widoretno; Serafinah Indriyani; Chairani Martasari; Rikza Hakin
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i1.714

Abstract

When obtaining plantlets, embryo maturation and simultaneous germination are important steps in plant micropropagation via somatic embryogenesis. Several studies have shown that the use of carbohydrates as a carbon source plays a significant role in inducing somatic embryo development in certain plant species. This study aimed to establish a somatic embryo maturation protocol for Batu 55 Mandarin (C. reticulata Blanco.) by examining the effect of various types of sugar and concentrations. The results showed that the type and concentration of sugar added to the medium affect the somatic embryo maturation of Batu 55 Mandarin. Galactose and maltose enhance somatic embryo maturation more effectively than does sorbitol. The combination of galactose or maltose with sorbitol was able to improve somatic embryo maturation more effectively than galactose or maltose alone. The combination of galactose and sorbitol enhanced the maturation of somatic embryos more effectively than did change the concentrations of maltose or sorbitol. It can be concluded that sugar type and concentration had effects on citrus somatic embryo development. The combination of sorbitol (36.5 mM) with galactose 73 mM was able to augment citrus somatic embryo maturation more effectively than the other concentrations applied.
Effect of Amending Organic and Inorganic Fertilizer on Selected Soil Physical Properties in Entisols Mohd Nizar Khairuddin; Isharudin Md. Isa; Abd. Jamil Zakaria; Abdul Rahym Abdul Rani
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1087

Abstract

Application of organic amendment has become a substantial option and it is originated from oil palm waste. The integrated application of organic and inorganic amendment on crops could effectively gain high yield of production. After application of treatments, variables such as pressure head, water content, hydraulic capacity, hydraulic conductivity, and diffusivity are important to determine and observe its effect on soil physical properties using RETC model. This study was conducted to investigate the influence of organic and inorganic amendments on Rasau soil series (Entisols). The comparison was made on the effect of organic amendments (Biogreen–BG and treated POME Sludge-TPS), and NPK fertilizer, and on maize yield which two of the treatments are the combination of organic and inorganic amendments (BG+NPK and TPS+NPK). The soil water holding capacity for NPK+TPS treatment was the highest compared to the control treatment. Hydraulic conductivity (Ks) was shown higher in NPK+BG treatment compared to the other treatments. In addition, the soil physical properties measurement in each treatment improved the soil hydraulic capacity uptake and moisture content. The combination of organic and inorganic fertilizer has shown a significant result in improving soil hydraulic properties compared to the NPK and control treatment.

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