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Natural Science: Journal of Science and Technology
Published by Universitas Tadulako
ISSN : 25411969     EISSN : 23380950     DOI : -
Core Subject : Science,
Computer Science & IT
Jurnal Natural Science online diterbitkan oleh Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) Universtas Tadulako. Jurnal ini menerbitkan artikel-artikel ilmiah hasil penelitian dalam bidang-bidang ilmu pengetahuan dan teknologi.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)" : 8 Documents clear
BACTERIAL MER GENES ARE POTENTIAL GENETIC MATERIAL FOR BIOREMEDIATION ON MERCURY POLUTION Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

Remediation over mercury-contaminated areas become major concern and needs to be solved. Recently, various parts of ecosystem were contaminated because of increase of mercury emission into environment. Methyl-mercury is the most toxic form, which commonly found in contaminated area. This pollutant was rapidly increased due to uncontrolled emission of mercury from power plan, gold mining process and from other sources. The one possible and reasonable method in mercury bioremediation is by using genetically modified plant expressing bacterial-mercury resistance gene. Group of Mer gene was characterized encode mercury transporter and mercury detoxification protein in bacteria. On this study, Blast search on database based on known Mer protein sequence, it shows that these proteins were conserved among bacteria. These groups of genes are potentially to be cloned and transformed into plant for phytoremediation purposes. In order to enhance mercury uptake and reduce its toxicity, multiple genes transformation are considered to be effective approach for transgenic plant construction. This transgenic plant should survive in medium with high concentration of mercury and it should have activities in mercury uptake, detoxification, and Volatilization.
KAJIAN TEKNOLOGI PRODUKSI BIOGAS DARI SAMPAH BASAH RUMAH TANGGA Mappiratu, Mappiratu; Sikanna, Rismawaty
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

The investigation about study the biogas production Technology from household waste has been done. This research aims to determine the starter concentration, water and household waste ratio to produc highest biogas, and also to find the fermentation time produce biogas with hight rendement. It was reache by applying 5 levels of cow dung starter concentration and 5 levels of water and household waste ratio i.e 0%, 6,25%, 12,5%, 18,75% and 25% and 1:2, 1:2,5, 1:3, 1:3,5 and 1:4 (b/v) respectively. The fermentation time was 3 days. The result showed that the higher cow dung concentration the lower biogas production. The highis biogas production (971,4 mg/L) was found with 6,25% of the starter. The increasing water and household waste ratio would decreased biogas production, and the using of 1:3,5 and 1:4 didn?t produce any biogas. The highest biogas production was found with 1:2 of water/household ratio i.e (631.29 mg/L). the fermentation time which produce the highest biogas rendement was found with 1 day of fermentation time
STUDI PENYEBARAN PENYAKIT FLU BURUNG MELALUI KAJIAN DINAMIS REVISI MODEL ENDEMIK SIRS DENGAN PEMBERIAN VAKSINASI UNGGAS Murwanti, Ica; Ratianingsih, Rina; Jaya, Agus Indra
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

Program vaksinasi unggas yang dilakukan untuk menghambat penyebaran penyakit flu burung dapat dikaji melalui model matematika. Dalam penelitian ini penyebaran penyakit tersebut di amati melalui revisi model endemik SIRS dengan memperhatikan pemberian vaksinasi pada unggas. Hal ini dilakukan dengan merevisi kompartemen penyebaran penyakit tersebut yang selanjutnya digunakan untuk menentukan persamaan pembangun model yang  direpresentasikan dalam sistem persamaan diferensial. Sistem tersebut menggambarkan  interaksi antara kelompok manusia dan kelompok unggas. Analisa kestabilan sistem di kedua titik  kritisnya dilakukan dengan menentukan nilai eigen sistem yang diperoleh dari determinan matriks jacobi  linearisasi sistem di masing-masing titik kritis. Kestabilan sistem selanjutnya diamati dibidang (  yang dibagi oleh kurva parameter   . Kedua parameter tersebut secara berturut-turut menyatakan tingkat vaksinasi unggas dan tingkat kematian alami populasi unggas. Kestabilan di titik kritis kedua membutuhkan syarat  bahwa  tingkat kematian manusia karena flu burung ( ) harus lebih kecil dari  tingkat kelompok manusia yang telah sembuh dari flu burung terjangkit penyakit kembali ( . Hasil simulasi memperlihatkan bahwa penyebaran penyakit flu burung dapat diturunkan dengan program vaksinasi bila tingkat vaksinasi unggas lebih besar dari tingkat kematian alami unggas
DISTRIBUSI LOGAM MERKURI PADA SEDIMEN LAUT DI SEKITAR MUARA SUNGAI POBOYA Purnawan, Sandi; Sikanna, Rismawati; Prismawiryanti, Prismawiryanti
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

An investigation about Mercury metal content in marine sediment around Poboya stream has been done. The sediment samples were collected at 5 points of Poboya stream with 0; 15 and 30 days of time variations. The physical conditions of the water was analyzed i.e pH, temperature and salinity. The result showed that the lowest and the highest Mercury metal content (0,0103 and 0,185 mg/Kg) were found at point E and C respectively. It was found at the second and the first sampling accordingly. There was no significant increase of Mercury accumulation by time. In general, the Mercury metal content in the sediment around Poboya stream was still under government rule.
SINTESIS BIOSURFAKTAN PALMITIN ETANOLAMIDA MENGGUNAKAN BIOKATALIS LIPASE IMOBIL GETAH PEPAYA Hendra, Hendra; Rahman, Rahman; Nurhaeni, Nurhaeni
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

Biosurfaktan palmitin etanolamida diperoleh dari reaksi antara asam palmitat dan etanolamina dengan menggunakan katalis enzim lipase imobil getah pepaya dan pelarut organik n-heksan. Pengaruh enzim lipase dapat dianalisa dengan menggunakan katalis enzim lipase ekstrak getah pepaya yang telah diimobilisasi dan reaksi tanpa katalis sebagai pembanding. Telah dilakukan tiga variasi untuk mendapatkan kondisi optimum reaksi masing-masing adalah variasi waktu, konsentrasi enzim imobil dan rasio asam palmitat terhadap etanol amida. Dari ketiga variasi tersebut maka waktu 72 jam, 12,5% enzim imobil dan rasio 1 : 7 memberikan hasil yang optimal dan hasil dilihat dari besarnya bilangan ester yang diperoleh
INDUKSI KALUS KLON KAKAO (THEOBROMA CACAO L) SULAWESI 2 PADA MEDIUM MS DENGAN PENAMBAHAN 2,4-D, BAP DAN AIR KELAPA Urfiana, Urfiana; Haliana, Haliana; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

Sulawesi 2 cacao clone is one of the clones which grown by local farmer in Central Sulawesi. One problem in cacao development in this time is the availability of high quality of seeds. One posible way in overcoming of this problem is through the application of tissue culture techniques via embryogenesis. The early stages of embryogenesis is callus induction, which aim to get emryonic callus cells and it can be a model in cacao research. This study was conducted over March and June 2013 in the Tissue Culture Laboratory, Faculty of Forestry Tadulako University. Explant that used was Stamen of flower of Sulawesi 2 cacao clone. This experiment was based on a complete randomized design (CRD) with 4 treatments, and it were repeated 3 times. On every single unit was growth 10 of explants. Different medium as treatments tested were: MS0 + 2 ppm 2,4-D + (15%) Coconut water (MK1), MS0 + 2 ppm 2,4-D + 0.2 ppm BAP + (15%) Coconut water (MK2) , MS0 + 3 ppm 2,4-D + (15%) coconut water (MK3), ms0 + 3 ppm 2,4-D + 0.2 ppm BAP + (15%) coconut water (MK4). Callus development were observed based on the emergence of callus, the percentage of explants producing callus, and cell callus morphology. Callus induction was done under dark condition and  temperature of 26º C. The results showed that all of the tested treatments can induce the callus of cacao. The best medium was MS medium + 3 ppm 2,4-D + (15%) Coconut water (MK3), characterized by the appearance of white, callus in intermediate-type, the callus mass was relatively larger than its under others treatments. Under this treatment, explant can produce uniform-relatively big cell and active in proliferation in 10 days after culture
PELABELAN TOTAL SISI AJAIB SUPER (TSAS) PADA GABUNGAN GRAF BINTANG GANDA DAN LINTASAN Sudarsana, I Wayan; Noviana, Noviana; Musdalifah, Selvy; Kasim, Anita Ahmad
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

An edge-magic total (EMT) labeling on a graph G(V,E) with the vertex set V and the edge set E, where |V| = p and |E| = q, is a bijective function ?: V E {1, 2, 3, ..., p + q} with the property that for each edge (xy) of G, ?(x) + ?(xy) + ?(y) = k, for a fixed positive integer k. The labeling ? is called a super edge magic total (SEMT) if it has the property that for each vertex obtain the smallest label, (V) = {1, 2, ..., p}. A graph G(V,E) is called EMT (SEMT) if there exists an EMT (SEMT) labeling on G. Study on SEMT labeling for the union of stars and paths initiated by Figueroa-Centeno et al. [2] with graph form . Furthermore, an investigation will be conducted on SEMT labeling of double stars and path, that are 2 ; 2 ;    2  and 2 . We obtain that the graphs presented above are SEMT with the magic constants k = , , and , respectively
PENGGUNAAN MALTODEKTRIN UNTUK MENINGKATKAN MASA SIMPAN LIKOPEN BUAH SEMANGKA (CITRULLUS VULGARIS SCHARD) sukriadi, sukriadi; Mappiratu, Mappiratu; Nurhaeni, Nurhaeni
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

Research using maltodextrin as the coating in order to increase the shelf life of watermelon lycopene has been done. This study aims to estimate the shelf life of watermelon lycopene capsules in packaging with the addition of maltodextrin coating. Achievement of the goals has been the separation of lycopene from watermelon flesh, followed by drying the product of lycopene using solar dryers, extraction of pure lycopene lycopene separation using a solvent mixture of N coarse-Hekasana: Aceton with a ratio of 2: 1, followed by mixing pure lycopene with adsorbent maltodextrin with a ratio of 25: 75% and storage in an oven at 40 and 50oC. Lycopene levels are determined every 24 hours for 10 days using the spectrophotometric method. The resulting data is used to predict the shelf life of lycopene in capsule packaging. The results obtained indicate a shelf life of lycopene at 15, 20, 25, 30, 35, 40, 45, 50, 55, and 60 ° C  are 631, 549, 478, 416, 362, 315, 276, 240, 209 and 181 days respectively.

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