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Jurnal Biosains Pascasarjana
Published by Universitas Airlangga
ISSN : 14121433     EISSN : 26214849     DOI : http://dx.doi.org/10.20473/jbp.v22i1.2020.1-10
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology
Jurnal Biosains Pascasarjana is published not only for the publication of research results from graduates, as one of the graduation requirements but also for public that contains a discussion of the natural content, responses of living things, and their environment.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
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Search results for , issue "Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA" : 10 Documents clear
The Effect Of Secang The Effect of Secang (Caesalpinia sappan) Masks on the Healing of Acne (Acne vulgaris) in Women Aged 21–25 Years Old Maasyitoh Sari Latifah
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.1-7

Abstract

Introduction: Acne can be caused by the presence of acne-causing bacteria. Currently, various studies of acne treatment with herbal ingredients both topically and orally have been carried out with the aim of reducing acne-causing bacteria. Several studies have shown that the active compound of Secang, namely brazilin extracted with methanol and 50% ethanol in Propionibacterium acnes, has been shown to have anti-bacterial, lipase inhibitor, and antioxidant activity. So that researchers are interested in conducting research on "The Effect of a Secang Mask (Caesalpinia sappan) on Healing Acne (Acne vulgaris) in Women Aged 21-25 Years". Material and Methods: This type of research is a paired numerical comparative analytical study using experimental methods and data collection methods with repeated measurements of Pretest-Posttest Control Group Design. 15 samples with acne were examined before given Secang mask with a composition of 0.5 grams of secang extract and 9.5 grams of rice flour. data was taken every 2 weeks for 4 weeks. The research data obtained will be presented in tabular form and then tested with the T-test in analyzing the results of the differences between pre-test and post-test Results: Based on the results of the study the effect of a secang mask (Caesalpinia sappan) on the healing of acne (Acne vulgaris) in women aged 21-25 years which was carried out for 30 days with a frequency of once every 2 days showed a significant decrease in the average total number of acne lesions (p = 0.00) compared to posttest, with a mean decrease of 33.38. Conclusion: From these statistical results, it can be concluded that secang mask therapy can improve acne healing in women aged 21-25 years which is characterized by a decrease in the total number of acne lesions, both comedones and inflammatory acne.
Effectivity of Shark Cartilage (Carcharhinus sorrah) Ointment Extract on Growth of Lizard (Mabouya multifasciata kuhl) Regenerated Tail Rakhmiyati Rakhmiyati
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.31-42

Abstract

Shark (Carcharhinus sorrah) is a fish whose entire endoskeleton is composed of cartilage. Fish cartilage is widely used for treatment because shark cartilage (SC) contains glucosamine (GS) and chondroitin sulfate (CS) compounds. This study aims to determine the benefits of glucosamine and chondroitin sulfate derived from SC extract on lizard tail regeneration. The study was initiated by testing the characteristics of the SC extract ointment which included tests of spreadability, pH, viscosity and adhesion of the ointment. The effectiveness test of SC extract ointment was done using 36 lizards that were autotomized and divided into 4 treatment groups. The treatment in the form of SC extract ointment with concentrations of 0% (control), 5%, 10%, and 15% was applied to the autotomized tail every morning and evening. The lizards were then kept for 7; 18; and 35 days. The test data for the physicochemical character of the SC extract ointment were analyzed using one-way ANOVA with a significance level of 5% (p<0.05) and the lizard regeneration tail length data were analyzed using a two-way ANOVA with a significance level of 5%. (p<0.05). The result of the research showed that there was a significant difference in the physicochemical character of the spread of SC extract ointment, meanwhile the other physicochemical characters showed no significant difference. The SC extract ointment used in this study was not effective in the growth of lizard regenerate tails.
Estimation Age of Bloodstain Using Smartphone Application on Colorimetric Analysis Reinhard Saliama
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.15-23

Abstract

Advances in technology should be able to help the work of forensic experts, especially in solving criminal crime cases. Estimated age of bloodstains is an important factor in forensic analysis. This study aims to identify the age of bloodstains using the colorimetric method and observe changes in the color of bloodstains on the surface of the material based on the results of photos using a smartphone camera. This study is an experimental study with a control time series design, where bloodstain samples are observed in a certain period longitudinally. Bloodstains were dripped on five different material surfaces, namely ceramics, glass, cardboard, paper, and newspapers, then observations and taking photos of bloodstains for 72 hours. Estimation of the age of bloodstains using artificial neural tissue by the backpropagation method. The results showed that the artificial neural network that was built was able to predict the age of bloodstains based on time classification. In addition, there is a significant influence of the color of bloodstains on the five surfaces of ceramic, glass, cardboard, paper and newspaper materials, namely. 63.7%; 84.3%; 84.6%; 68.2% and 86.6%.
Bioencapsulation Artemia with Bacillus subtilis and Sodium Alginate on Total Hemocyte and Survival of Litopenaeus vannamei Infected with Vibrio parahaemolyticus Anggraini Widihastuti; Woro Hastuti Satyantini
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.8-14

Abstract

Disease control in shrimp culture is mostly done by using probiotics such as B. subtilis. Immunostimulants are also widely used to improve the white shrimp immune system. One of the immunostimulants that can be used is sodium alginate. The purpose of this study was to determine the effect of bioencapsulation of Artemia with the combination of B. subtilis and sodium alginate on total hemocytes and the survival of white shrimp infected with V. parahaemolyticus. This study used a completely randomized design (CRD) with 5 treatments and 4 replications. Hemocyte total and survival were measured at H0, H7 after bioencapsulation, H9 one day post-infection, and H16 seven days post-infection. The results showed that the total hemocytes H7 and H9 in treatment P3 were significantly different (p<0.05) against all treatments, while the survival of white shrimp in H9, P1 and P3 treatment was not significantly different (p>0.05), to the treatment of K- and P2. The conclusion of this study was that the bioencapsulation of Artemia with the combination of B. subtilis and sodium alginate could increase total hemocytes and the survival of PL white shrimp infected with V. parahaemolyticus.
Effect of Pesticide Poison on Post-Mortem Interval Determination Based on Entomological Study Barizah Luay Widyana
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.49-59

Abstract

Determination of post-mortem intervals (PMI) is a major key in forensic investigation. On the discovery of a decomposed corpse, a post mortem examination is difficult. PMI can be made by observation of insects that appear around the corpse, called forensic entomology. Forensic entomology carried out by analyzing the species of larvae and calculating the age of insects so that the time of death can be estimated. Forensic entomology has been developed in toxicological examinations so that insects found can be used as toxicological samples. The cause of death can be due to poisoning. Poisons often used in case of poisoning or suicide is pesticides. This research is a Systematic Review, a study that takes and collects data from various previous studies related to the chosen theme. The study was conducted according to protocol and registration with reference to PRISMA and JBI. Literature searches are obtained from online databases. The results were obtained by 21 studies after selection and elimination in accordance with protocol. The most accurate determination of the post mortem interval depends on post-death changes and the decomposition process. Some types of pesticides can cause the decomposition process to take place faster but some other types of pesticides can slow down the decomposition process. The content of toxins in the body ingested has an effect on the development and growth of necrophagous insects. The growth of flies will be stunted or even stopped, the presence of toxins in the corpse can be a contributing factor in the determination of the post-mortem interval. Deaths caused by toxins still need to be further researched because different types of toxins can show different side effects on the body.  
Toxicity Evaluation of Iodine Disinfectant Inclusion on Mortality and Growth of Artemia franciscana Nauplii Reza Istiqomatul Hidayah; Woro Hastuti Satyantini; Sapto Andriyono
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.24-30

Abstract

Artemia franciscana nauplii is a source of nutrients and pathogen vectors due to the non-selective nature of the filter feeder. Prevention of pathogens is done by adding iodine disinfectant to suppress the emergence of bacteria. One way to find out if a substance is toxic is by testing the toxicity and lethal concentration (LC50) on A. franciscana nauplii. The aim of this study was to evaluate the disinfectant effect of iodine and determine the lethal concentration of iodine in 50% of A. franciscana nauplii (LC50). Brine Shrimp Lethality Test (BSLT) method using Artemia as test animal. The results of the toxicity test with several doses of iodine showed that the toxic threshold value for 96 hours was 20.31 ppm. The results were supported by the presence of stunted growth at a dose of 100 ppm by measuring the length and weight. The conclusion of this study is that the administration of iodine at several concentrations is toxic to A. franciscana nauplii if it exceeds 20.31 ppm and affects the growth of A. franciscana nauplii.
Effect of the Processing and Storage of Thrombocyte Concentrate on the Acidity (Ph) Levels and the Amount of Platerombocites Muhamad Syamsul Arif Setiyo Negoro
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.60-65

Abstract

 In order to maintain good quality of trombho concentrate (TC)  based on pH level and platelet count, to store it at 22 ± 2°C for 3 – 5 days in vitro. is to determine the effect of the processing and shelf life of platelet concentrate on pH levels and platelet counts. The research design was quasi-experiment pre and posttest group design.. there was no significant decrease in the pH level of the TC which was processed from whole blood. The  pH level of the TC which was processed from thrombopheresis experienced a significant increase in p value of 0.00. There was a significant increase in the number of platelets in the TC which was processed from whole blood, whereas in the TC which was processed from thrombopheresis it decreased significantly. that the shelf life and the processing of platelet concentrates affect the pH level and the number of platelets
An In vitro Tuberculosis Granuloma Model in M. tuberculosis H37Rv: In vitro Tuberculosis Granuloma Model in M. tuberculosis H37Rv Talita Shofa Adestia
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.66-73

Abstract

M. tuberculosis is a bacterium that has many evasion mechanisms against the immune system, one of them is the formation of granulomas which is beneficial for the bacteria’s survival. The granuloma structure is useful for limiting the spread of M. tuberculosis and localizing infection, also considered as part of M. tuberculosis life cycle that successful fighting the body's immune system. This study aims to look at the formation of an in vitro tuberculous granuloma model. This study used the True Experiment type which began with blood sampling, PBMC isolation, macrophage isolation, MOI 10 making and granulomas making. Granulomas were observed on day 0, 1, 4, 7, 9, 10 and 14. Cells started to aggress on day 1 and giant cells were seen on day 4. The granuloma formed on day 9 and was maintained on day 10, however, the granuloma ruptured on day 14 which caused the cells to re-aggregate. Keywords: Granuloma, M. tuberculosis, PBMC
Training for Disaster Response Youth Cadres About Post Earthquake Injury Using Student Centered Learning (Scl) Approach septyana eka rahmawati; Eka Rudy Purwana; Hardiansyah hardiansyah; Dewi Purnamawati
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.74-78

Abstract

Efforts to deal with crises due to disasters or so-called disaster management are a series of activities that began before the occurrence of outbreaks and disasters which were carried out through prevention, mitigation (mitigating/mitigating the impact) and preparedness in dealing with outbreaks and disasters. In general, disaster management is aimed at preventing and limiting the number of victims, so there must be early intervention efforts made by people exposed to training so that the severity of the disaster does not occur. due to the earthquake at SMA Negeri 8 Mataram with a student centered learning (SCL) approach. This study uses a quasi-experimental design with one pre and post test design. A total of 40 Disaster Response Cadre (KATANA) cadres were given disaster response cadre training and disaster preparedness was measured before and after the training. Data analysis used paired t test. The results show that there is an effect of post-disaster intervention disaster response training on disaster preparedness with a p-value of 0.000 <0.05. Conclusion, the results of this study are expected to be a program innovation for youth cadres to improve disaster preparedness.    
Effect of Storage Time on DNA Content and Purity in Lip Print Ahmad Yudianto; Titik Erliyah; Abdul Hadi Furqoni; Indah Nuraini; Qurrota A'yunil Huda
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.43-48

Abstract

Forensic experts in uncovering the truth of a case must go through identification, documentation, and collection of evidence. Sometimes at a crime scene, lip prints are found on the surface of an object such as the mouth of a plastic bottle. Lip print research on plastic bottle mouths was carried out for 1, 3 and 7 days. There are 21 samples with details of 3 as controls, 6 samples for the first day, 6 samples for the 3rd day, and 6 samples for the 7th day. DNA extraction using DNAzol, quantification using UV spectrophotometer, and DNA amplification using STR primers, namely TPOX and TH01 loci. For DNA visualization using acrylamide gel. The average results of lip print DNA levels for 1, 3, and 7 days were 369.82 g/ml, 550.72 g/ml, 318.02 g/ml. The average yield of lip print DNA purity for 1, 3, and 7 days was 1.79; 1.78 and 1.79. From the results of DNA quantification, the lowest and highest DNA levels were taken on days 1, 3, and 7. Of the 6 samples and 3 controls amplified using the TPOX and TH01 loci, the results were clearly visible on the acrylamide gel band.

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