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Jurnal Biologi Universitas Andalas
Published by Universitas Andalas
ISSN : -     EISSN : 23032162     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 295 Documents
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Above Ground Biomass Estimation of Syzygium aromaticum using structure from motion (SfM) derived from Unmanned Aerial Vehicle in Paninggahan Agroforest Area, West Sumatra Try Surya Harapan; Ahsanul Husna; Thoriq Alfath Febriamansyah; Mahdi Mutashim; Andri Saputra; Ahmad Taufiq; Erizal Mukhtar
Jurnal Biologi Universitas Andalas Vol 9, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.1.39-46.2021

Abstract

Above ground biomass (AGB) is all living organic matters above the soil including stem, seed and leaves. This study aimed to estimate the individual clove (Syzygium aromaticum) and it’s above ground biomass using Unmanned Aerial Vehicle in the Agroforestry area in Paninggahan, West Sumatra. This study used a photogrammetry method to calculate trees and estimated the AGB. We detected 257 numbers of trees based on aerial image analysis and observed 270 after we validated on ground check in the field. The result was slightly different between estimated AGB from UAV and observed AGB from our ground validation. The estimated AGB was 5.9 ton/ Ha where the surveyed AGB was 5.6 ton/Ha. The difference between estimated AGB and observed AGB was 0.3 ton/Ha.
Diversity of Bivalvia, Gastropoda and Holothuroidea in Intertidal Zone of North Javan Sea Coastal, Indonesia V. Rohmayani; E. Tunjung Sari M.; Nurhidayatullah Romadhon; H. Ichda Wahyuni
Jurnal Biologi Universitas Andalas Vol 9, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.1.1-7.2021

Abstract

This study aims to know diversity of Bivalvia, Gastropoda and Holothuroidea. Measures used in this study were Density Index (D), Diversity Index (H’), Evennes Index (E’) dan Important Value Index (INP). This study used transect sampling method by 1 × 1 meter quadrant. Target species of this study were member of Bivalvia, Gastropoda and Holothuroidea that inhabit in intertidal zone of north Javan Sea coastal. This intertidal zone was divided into 3 zone; zone I is 50 meter, zone II is 75 meter and zone III is 100 meter from coastal line to sea. Total sample of this species were 1064 individual, that divided into 7 species of Bivalvia, 10 species of Gastropoda and 2 spesies of Holothuridea. The highest Diversity Index was for Gastropoda in zone I (253 individual/m2). Diversity Index was in moderate category (1< H’ < 2) while the zone III was the highest. Evennes Index of all zones were relatively similar. The highest Important Value Index was species of Clypeomorus clypeomorus, that has value 53,06%.
The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro Maya Sari; Mayta Novaliza Isda
Jurnal Biologi Universitas Andalas Vol 9, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.1.8-17.2021

Abstract

The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting.
Ethnomedicinal Study of the Use of Zingiberaceae by the Mentawai People in Siberut, West Sumatra, Indonesia Nurainas Nurainas; Ratna Sulekha; Zuhri Syam; Samantha Lee; Syamsuardi Syamsuardi
Jurnal Biologi Universitas Andalas Vol 9, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.1.25-29.2021

Abstract

The Mentawai archipelago is situated to the west of mainland Sumatra and is part of the West Sumatra province. The Mentawai people are indigenous to this archipelago and are well known for their traditional healing practices performed by their Sikerei healers. Only a few studies on the traditional plant medicines of the Mentawai people have been published, which mostly suggest that Zingiberaceae is one of the most widely used families. This study examines the indigenous knowledge of Zingiberaceae by the Mentawai people living in Siberut. Field surveys were undertaken at four locations in the island where the Sikerei healers were interviewed directly to obtain information about medicinal treatments using plants from the Zingiberaceae family. Voucher specimens were collected, dried and deposited at the Herbarium of Andalas University (ANDA), Padang, West Sumatra. The study suggests that at least 32 Zingiberaceae species are used in the Mentawai’s traditional medicines. The floristic aspects, the plant part used, and the type of disease treated are discussed.
Distribution Pattern and Mapping of Invasive Alien Species Bellucia pentamera in Conservation Area of PT. Tidar Kerinci Agung (TKA) Solok Selatan Uswatul Inayah; Solfiyeni Solfiyeni
Jurnal Biologi Universitas Andalas Vol 9, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.1.30-38.2021

Abstract

Bellucia pentamera is one of the most dangerous invasive alien species for environment. This species had been invaded many forests in Indonesia. This species would invade more area due to deforestation. The objectives of this study were to know the mapping and distribution pattern of B. pentamera; to know the effects of distance from road and light intensity to distribution of B. pentamera. This study was conducted from March to August 2020 in Conservation Area of PT. TKA Solok Selatan using belt transect method by plotting 20x50 m2. Data was analyzed using Morishita Index and Linear Regression. Distribution pattern of B. pentamera in PT. TKA was clumped, showed by 1.17 of Morishita index. Seedlings and saplings of B. pentamera were dominant at the edge of conservation forest while trees were distributed from the middle to inside of the forest. Distance from road gave positively effects and significantly towards distribution of B. pentamera showing result 0.702 of R2 and 0.007 of p-Value. Light intensity did not give effects significantly towards distribution of B. pentamera showing result 0.0806 of R2 and 0.427 of p-Value.
RAPD Primers Selection for Genetic Variation Analysis of Banana Plant (Musa spp.) I Ketut Catur Wiguna; Made Pharmawati
Jurnal Biologi Universitas Andalas Vol 9, No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.47-53.2021

Abstract

Identification of genetic diversity using molecular markers is important for basic information for plant conservation. Banana is a fruit bearing plants that important for food sources in human life. This study aimed to determine the optimum conditions of  PCR-RAPD reaction and RAPD primers that suitable to amplify DNA fragmens. DNA isolation was done using modified of CTAB and chloroform isoamil alcohol. The samples used was young leaves of nine banana cultivar plants. Optimation was done using variety of DNA and MgCl2 concentration. Eight primers produced by Operon Primer Technology were tested. The DNA genomic concentration obtained was in the range of 23,3 ng/µl – 70 ng/µl. The optimum conditions of PCR-RAPD of banana plants that produce clear band were 50 ng/µl DNA template, 3 mM MgCl2 with the number of thermal cycles was 40 x. There were six RAPD primers that successfully amplified DNA : OPA 02, OPA 04, OPB 12, OPD 20, OPH 01, and OPH 03. The primer OPA-04 had the lowest resolving power value (4,4) , while  OPH 01 had the highest (11,3) resolving power.
Characterization And Screening Of Protease, Amylase, And Cellulase From Phylloplane Fungi Isolates Of Avicennia marina (Forssk.) Vierh. Wahyu Aji Mahardhika; Warih Ramadhany; Arina Tri Lunggani
Jurnal Biologi Universitas Andalas Vol 9, No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.54-59.2021

Abstract

Mangrove (Avicennia marina) is an important mangrove species, because it has many benefits for human life, including in the ecological scope as a source of food and medicine. These plants can also be used as a source of symbiont mold isolates which can be developed as an alternative to produce bioactive compounds, one of which is enzymes. Enzymes are protein compounds that can catalyze all chemical reactions in biological systems. This study aims to determine whether mangrove symbiont fungi (A. marina) can produce amylase, protease and cellulase enzymes. This research was conducted using a method based on enzyme activity, namely amylase activity with lugol iodine staining, protease activity and cellulase activity with congo red staining. on agar media enriched with 1% skim milk, 1% starch and 1% CMC. The results showed that 4 isolates were able to show potential enzymatic activity to be developed as agents for enzyme production.
Kidney Histology of Wistar Rats (Rattus norvegicus Berkenhout 1769) Due to White Coffee Yola Alunsa Agi; Titrawani Titrawani
Jurnal Biologi Universitas Andalas Vol 9, No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.60-67.2021

Abstract

Coffee is a drink that is very popular with the people of Indonesia and in other countries. Coffee has many benefits but on the other hand, coffee can also damage health, the dangerous content in coffee is caffeine and cafeeic acid. The effect of coffee consumption is still a matter of controversy, one of which is the effect on the kidneys because the kidneys are the main excretory organ for removing metabolic waste from the body. The kidney is the second organ after the liver, which is most often the target of damage by chemicals. This study aims to determine the effect of multilevel doses of white coffee on the histological picture of rats' kidneys. This research is a completely randomized design study with four 4 treatments and four 4 replications. Consists of K (distilled water), P1 (0.36 ml), P2 (1.08 ml) and P3 (2.16 ml). The results of this study showed that macroscopic observations of the administration of white coffee civet coffee solution with different doses showed that the kidneys looked normal. It is characterized by the shape of the kidney like a pea and brownish-red in color. The P0 group was significantly different from the P1, P2, P3 groups. The P1 group was significantly different from the P0 group and the P2 group, P3. The P2 group was significantly different from the P0, P1, and P2 groups. The P3 group was significantly different from the P0, P1, and P2 groups. This study concluded that white coffee in multilevel doses affects the histopathology of kidneys in rats.
In Vitro Callus Induction in Tacca (Tacca chantrieri Andre) Leaf Explants on Murashige and Skoog Media with Different Concentrations of Sucrose Mayta Novaliza Isda; Melda Jannatul Salsabilla
Jurnal Biologi Universitas Andalas Vol 10, No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.1-9.2022

Abstract

Tacca chantrieri belongs to the family Taccaceae has black flowers and has a long filiform that looks like a bat. T. chantrieri contains phytochemicals in the form of spritosol saponins used as traditional medicine by the people of China and Thailand. The amount of land clearing, forest exploitation and habitat destruction resulted in a reduction in the number of T. chantrieri, so T. chantrieri was propagated to maintain its sustainability. One way that can be used is the in vitro culture technique, namely callus culture. Callus culture is an early stage of in vitro culture technique where this stage aims to produce and multiply callus cells. The purpose of the study is were to determine the effect of different sucrose concentrations on callus induction from T. chantrieri leaf explants and determine the best sucrose concentrations for callus culture from T. chantrieri leaf explants on Murashige and Skoog (MS) media. This study used a single factor completely randomized design (CRD), namely sucrose concentrations 0 g/l, 10 g/l, 20 g/l, 30 g/l, 40 g/l, 50 g/l with five replications. The results of this study showed that the administrations of sucrose with different concentrations on MS media had an effect on increasing callus induction in Tacca chantrieri leaves. The best sucrose concertations for callus induction of Tacca chantrieri leaves was the addition of 40 g/l sucrose at 20 days after planting, 60 % callus formation percentage, callus formed in the form of compact callus and produce yellow-white callus. Keywords: Tacca chantrieri, sucrose, callus, Murashige and Skoog.
Ethnobotanical Study of Ferns as Traditional Medicine in Central Siberut, Mentawai Island Nova Syafni; Amri Bakhtiar
Jurnal Biologi Universitas Andalas Vol 10, No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.10-14.2022

Abstract

West Sumatra Province has two ethnicities, namely Minangkabau and Mentawai. Both ethnicities have the capability to apply plants in folk medicine. This ethnobotanical study was conducted in Saibi Simokop village in three hamlets namely Sirisurak, Simoilaklak, and Saibi. The study was focused on the use of ferns in traditional medicine. Data collection was carried out using the snowball sampling method to seek the traditional healers (sikerei). The results showed that seven species of ferns were used for treatment of fever, inflammation, and poisoning. The ferns were included Cephalomanes javanicum (Blume) Bosch, Angiopteris evecta (G. Forst.) Hoffm., Dicranopteris linearis (Burm. F.) Underw., Diplazium esculentum (Retz.) Sw., Phymatosorus membranifolium (R. Br.) S.G. Lu, Lecanopteris sp., and Asplenium nidus L. The application of ferns in concoctions was administered topically and some were combined with oral administration.

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