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UJI KOMBINASI PUPUK ORGANIK DAN ANORGANIK DALAM POLA LEISA TERHADAP PERTUMBUHAN DAN HASIL TANAMAN SORGUM LOKAL PADA LAHAN MARGINAL Leomo, Sitti; Sutariati, Gusti Ayu Kade; Agustina, Agustina
Jurnal Agroteknos Vol 2, No 3 (2012)
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The experiment was aimed to know the effect of combination of organic and anorganic fertilizers on growth and yield of local sorghum crops on dry land. The experiment was conducted in Kambu District Poasia and Agrotechnology Laboratory, Faculty of Agriculture University of Haluoleo, from August to December 2011. The experiment was arranged on randomized block design (RBD), which consisted of 7 treatments namely: control (A), 5 tons ha-1 of organic fertilizer (B), anorganic fertilizer (200 kg ha-1 urea, 100 kg ha-1 SP36 and 50 kg ha-1 KCl) (C), organic + anorganic fertilizer full recommended dosage (D), organic fertilizer +1/2 recommended dosage of anorganic fertilizer (E), organic fertilizer + ¼ recommended dosage of anorganic fertilizer (F) and organic fertilizer + 1/8 recommended dosage of anorganic fertilizer (G). Each treatment was repeated 3 times, therefore overall there were 21 experimental units. Data obtained were analyzed using analysis of variance and followed by Duncan’s Multiple Range Test. The research results showed that the combination of organic and anorganic fertilizers provided a significant influence on growth and yield of sorghum crops. Treatment of organic fertilizer + ½ recommended dosage of anorganic fertilizer (organic fertilizer 5 ton ha-1 + anorganic fertilizer 100 kg ha-1 Urea, 50 kg ha-1 SP36dan 20 kg ha-1 KCl) gave better effects on the observed variables: plant height, stem diameter, leaf number, leaf area, dry weight of plant biomass, panicle length, fresh weight of panicle, grain weight and the weight of 1000 seeds of sorghum. Keywords: combination of organic and anorganic fertilizers, rhizobakteria, sorghum

POTENSI RIZOBAKTERI INDIGENOUS ULTISOL UNTUK MENGENDALIKAN PENYAKIT BUSUK BATANG PHYTOPHTHORA (Phytophthora capsici) PADA TANAMAN CABAI. Khaeruni, Andi; Sutariati, Gusti Ayu Kade; Rahman, Abdul
Jurnal Agroteknos Vol 1, No 1 (2011)
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The major problems to use the ultisol land in Southeast Sulawesi are low pH, poor nutrients and Fusarium wilt disease suppression. Plant growth-promoting and bioprotecting rhizobacteria (PGPBR) is one of the few possibilities to overcome these problems. The objectives of this experiment were to evaluate the potency of ultisol indigenous rhizobacteria to control Phytophthora stem rot disease on chili pepper. Evaluation was conducted using five isolates of rhizobacteria (ST17c, ST26c, SS15b, SS16b & SS29a), as biological control agents treatments, compared to control treatment. The results indicated that application of ultisol indigenous rhizobacteria on seed and chili pepper rhizosfer significantly increased plant height and reduced disease incidence and disease severity of Phytophptora stem rot disease in chili pepper, compared to control. The best result was by the application of ST26c isolate, resulted in no disease symptoms developed during this experiment and significant increase in plant height to 32.28 centimeter at six week after planting. Key words: rizobacteria, Phytophthora capsici, Phytophthora stem rot disease

PENGEMBANGAN BIOSENSOR ELEKTROKIMIA BERBASIS ENZIM ASETILKOLINESTERASE UNTUK ANALISIS RESIDU PESTISIDA PADA PRODUK PERTANIAN Mashuni, Mashuni
Jurnal Agroteknos Vol 2, No 2 (2012)
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Pesticide is one of dangerous chemical compounds in environment. Its existence has spread so it can cause negative effect for human. Pesticide compound is toxic in for nervous system so that a quick detection system is needed to protect human’s health and as a control for agricultural product. Therefore, we conducted a research of electrochemical biosensors development based on enzyme acetylcholinesterase in electrode to analyze pesticide residue so that a more superior and reliable biosensors can be obtained for pesticide residue analysis in foodstuff samples, especially for agricultural product. Residue analysis of carbamate and organophosphate in foodstuff sample requires a sensitive, selective, cheap- and-easy-operated, and quick-analyzed tools. A new research has been designed in making of biosensors electrode by using immobilization method of enzyme acetylcholinesterase (AChE) with the supporting materials of Acetate Selulose (AS) and Glutaraldehid (GA) in biosensor electrode connected with electrochemical transducer. Designed biosensors were characterized, including Nernst factor, the detection limit, response time, and period of use. Next, biosensors were tested in sample of vegetables and fruits. Expected result was a biosensor prototype with better work than pesticide determination with instrument of Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC). Biosensor development offers low cost, accurate, quick analysis time, and sensitive to detect pesticide residue. Keywords: : Pesticide, Biosensor, Electrochemical, Enzim, Sensitive

PENGARUH FUNGI MIKORIZA ARBUSKULA DAN NUTRISI ORGANIK TERHADAP PERTUMBUHAN TANAMAN CABAI MERAH BESAR (Capsicum annuum L.) Arma, Makmur Jaya; Risnawati, Risnawati; Gusnawaty, HS
Jurnal Agroteknos Vol 3, No 3 (2013)
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The research to study the effects of arbuscular mycorrhiza fungi (AMF) and organic nutrients to enhance the growth of chili has been conducted in Experimental Field, Faculty of Animal Husbandry and laboratory of Agrotechnology, Halu Oleo University, from June to November 2012. The research was based on the split-plot design with a randomized block design pattern (RAK) of two factors: Organic Nutrition as the main plot and AMF as subplot. Organic nutrients as the main plot consisted of three levels, namely: without organic nutrition (S0), 1 mL L-1 of water (S1) and 2 mL L -1 of water (S2); and AMF dose as subplot consisted of three levels, namely: without AMF (M0), 5 g plant-1 (M1) and 10 g plant-1 (M2). therefore, there were 9 combinations of treatments and each treatment combination was repeated three times to obtain 27 experimental units. Each variable was analyzed by analysis of variance, then followed by Duncan's Multiple Range Test (UJBD) at 95% confidence level. The results of research indicated that the best interaction of AMF and organic nutrient treatment was 10 g AMF plant-1 (M2) and 2 mL L-1 (S2) of organic nutrients. This treatment combination can improve growth on variables: leaf area, leaf area index and yield index of the chili plants. The best treatment for AMF independently was at 10 g plant-1 (M2) because it can promoted growth of plant height of the chili plants. The best treatment for organic matter independently was at 2 mL L-1(S2), because it can promoted growth of plant height of the chili plants. Keywords: FMA, organic nutrition, growth, chili

UJI KONSENTRASI CAIRAN PERASAN DAUN KENIKIR(Tagetes patula Juss) TERHADAP MORTALITAS ULAT PENGGULUNG DAUN (Lamprosema indica ) PADA TANAMAN UBI JALAR Rahayu, M; Pakki, Terry; Saputri, Ramlia
Jurnal Agroteknos Vol 2, No 1 (2012)
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This experiment was conducted at the Plant Pest and Disease Laboratory, taking place in October 2011 to Januari 2012. This research compiled based on completely randomized design (RAL) with 5 (five) treatments and 3 treatment. The treatment were control (P0), dilution of juice of leaf kenikir 10 mL/ water litre (P1), dilution of juice of leaf kenikir 15 mL/ litre (P2) dilution of juice of leaf kenikir 20 mL/ litre (P3), dilution of juice of leaf kenikir 25 mL/litre (P4), therefore were 15 treatment units. Parameter observed was insect mortality, observed at 12, 24, 36, and 48 hours after application (JSA). Research results indicated that highest average of leaf winder caterpillar mortality (Lamprosema indica) was at treatment P4 (dilution of juice of leaf kenikir 25 mL/ litre), equal to 63,33 % at observation of 36 JSA. Keyword: kenikir’s leaf, mortality, Lamprosema indica, sweet potato

UJI EFEKTIVITAS BEBERAPA MEDIA UNTUK PERBANYAKAN AGENS HAYATI Gliocladium sp. Gusnawaty, HS; Taufik, Muhammad; Wahyudin, Edi
Jurnal Agroteknos Vol 3, No 2 (2013)
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This research was carried out in the Laboratory of Pests and Disease, Agricultural Faculty of Haluoleo University, from October to December 2012. This research was arranged in A Completely Randomized Design (CRD), with 7 treatments, namely: Gliocladium sp. in sago waste (a), Gliocladium sp. in cashew seed wastes (b), gliocladium sp. in saw dust waste (c), Gliocladium sp. in corn medium (d), Gliocladium sp. in bran medium (e), Gliocladium sp. in rice medium (f) and gliocladium sp. in rice husk waste (g). The research results showed that media used had different effectiveness. The most effective medium for Gliocladium sp. Propagation was rice bran medium, based on Gliocladium sp. growth (100%), and the number of Gliocladium sp. conidium ( 2,0 x105/g). Keywords: effectiveness, Gliocladium sp., medium, propagation

PERKEMBANGAN DAN HAMBATAN MAKAN LARVA Crocidolomia pavonana YANG DIBERI SEDIAAN FRAKSI DIKLORMETAN KULIT BATANG Calophyllum soulattri Syahputra, Edy; Prijono, Djoko
Jurnal Agroteknos Vol 1, No 3 (2011)
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ABSTRACKThe objectives of this study were to evaluate the develovment time, and feeding inhibity of Crocidolomia pavonana larvae fed dichloromethana fraction of Calophyllum soulattri bark preparation. Fractionation of C. soulattri fraction was performed with vaccuum liquid chromatography. Bioassay was conducted against C. pavonana larvae by leaf-feeding method. Second-instar C. pavonana larvae were fed extract-treated broccoli leaves for 48 haurs, then were presented with untreated leaves until the surviving larvae reached the fourth-instar stage. The number of dead larvae was recorded. For feeding inhibity, preparation was assayed using leaf-disc choice and no-choice test towards third- instar C. pavonana larvae. The results showed that dichloromethan fraction of C. soulattri barks possessed strong insecticidal activity against C. pavonana larvae with LC50 of 0,06%. The dichloromethan fraction at 0,06%-0,09% could prolong development time of instar II-III larvae by 1,7-2,7 days. In choice and no-choice leaf disc methods, the dichloromethan fraction at 0,02%-0,075% inhibited feeding of instar III larvae by 54,8%-100%. Concerning with their potentiality, further studies are needed to identify insecticidal compounds in those active extracts.Keywords: Calophyllum soulattri, Crocidolomia pavonana, feeding inhibity, insecticidal activity

KAJI TINDAK PENGENDALIAN HAMA PENGGEREK BUAH KAKAO (Conopomorpha cramerella Snellen) DENGAN PESTISIDA NABATI Nuriadi, Nuriadi; Gusnawaty, HS
Jurnal Agroteknos Vol 3, No 1 (2013)
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This research aimed to know the effectivity of botanical pesticide in retarding cocoa pod borer. The study was conducted using completely randomized design, with 7 treatments and 3 replications. Total number of trial units was 21. The results of the study showed that the ingredient botanical pesticide that gave significant effect was ingredients III with interval 5 days/application which can limit attack of pod from 100 % to 24,78 % and intencity attack from 92,56 % to 11,25 %. Keywords : botanical pesticide, cocoa pod borer, intencity attack

RESPON BIBIT TANAMAN LADA TERHADAP APLIKASI MIKORIZA INDIGENOUS GULMA Hasid, Rachmawati; Halim, Halim
Jurnal Agroteknos Vol 1, No 1 (2011)
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The research on the response of pepper seedlings to the application of mycorrhiza indigenous of weed has been carried out at Sindang Kasih village of Konawe Selatan district on May until September 2008. The aim of this research was to study response of pepper seedlings to the application of mycorrhiza indigenous of weed. The research used randomized complete design with six treatments and three replications. The treatments were control (M0), 10 grams mycorrhiza indigenous of weed/polybag (M1), 15 grams mycorrhiza indigenous of weed/polybag (M2), 20 grams mycorrhiza indigenous of weed/polybag (M3), 25 grams mycorrhiza indigenous of weed/polybag (M4), 30 grams mycorrhiza indigenous of weed/polybag (M5). The parameters were number of leaves, percentage of infection of mycorrhiza to pepper root and relative field mycorrhiza dependency of pepper. The research showed that the highest number of pepper leaves at the end research was M1 with 12.65 leaves, percentage of infection of mycorrhiza to pepper root was M4 with 96.67 % and the lowest relative field mycorrhiza dependency was M2 with 19.74 %. Key words: mycorrhiza indigenous, weed, pepper, relative field myccorhiza dependency

EFEKTIFITAS INOKULASI ACTINOMYCETES DAN VAM DALAM MENGENDALIKAN PENYAKIT REBAH SEMAI (Sclerotium rolfsii ) PADA TIGA VARIETAS KEDELAI PADA MUSIM KEMARAU Gusnawaty, HS
Jurnal Agroteknos Vol 1, No 2 (2011)
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ABSTRACT The aim of the research was to know the effectiveness of actinomycetes and VAM inoculations in preventing the occurrence of infection of Sclerotium rolfsii on soybean at dry season. This research was conducted at the Phytophatology Laboratory, Mycology sub- Laboratory of the Plant Protection Faculty of Agriculture, University of Brawijaya, and in the trial plantation of Balai Benih Induk Palawija, Lawang, Malang subdistrict. This research used the Random Group Design (RGD) which consisted of two factors, namely (1) variety factor consisted of Ratai, Kaba and Burangrang; (2) inoculum factor, that were without actinomycetes and VAM inoculation, inoculation with actinomycetes, inoculation with VAM, combined inoculation of actinomycetes and VAM. The research result showed that the combined inoculation of actinomycetes and VAM could control the level of attack in three soybean varieties with the highest control effectiveness. Keywords: Actinomycetes, VAM, soybean, Sclerotium rolfsii

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