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UJI POTENSI TRICHODERMA INDIGENOUS SULAWESI TENGGARA SEBAGAI BIOFUNGISIDA TERHADAP Phytophthora capsici SECARA IN-VITRO Gusnawaty, HS; Asniah, Asniah; Taufik, Muhammad; Faulika, Faulika
Jurnal Agroteknos Vol 3, No 3 (2013)
Publisher : Jurnal Agroteknos

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This research was conducted in the Laboratory of Plant Pest and Disease, Department of Agrotecnologi, Faculty of Agriculture, Halu Oleo University Kendari, from May to August 2013. This study aimed to evaluate potential Trichoderma isolates indigeneous Southeast Sulawesi as biofungicide against Phytophthora capsici and Fusarium oxysporum in-vitro. The potential inhibitory test used multiple testing methods on PDA medium. The research design was a completely randomized design (CRD) consisting of 11 treatments (trichoderma isolates) with three replications. Variables measured were the inhibition of trichoderm a indigeneous on the growth of P. capsici and F. oxysporum. Results of the experiment showed that the trichoderma isolates were potential as biofungicide of P. capsici and F. oxysporum because they were able to inhibit the growth of pathogens in-vitro. All trichoderma isolates tested had the same potential as biofungicide against P. capsici, and isolate DKT, BPS, LKA, ASL, LTB, APS, DPA, LKO and DKP has the best potential as biofungicide against pathogenic F. oxysporum in-vitro. Keywords: F. oxysporum, inhibitory, indigenous of Southeast Sulawesi, P. capsici, trichoderma

PENGELOMPOKAN PATOTIPE Xanthomonas oryzae pv. oryzae ASAL SULAWESI TENGGARA MENGGUNAKAN PADI GALUR ISOGENIK IRRI Syair, H; Samirin, Samirin; Wijayanto, Teguh; Khaeruni, Andi
Jurnal Agroteknos Vol 2, No 1 (2012)
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This Research aimed at grouping of Xanthomonas oryzae pv. oryzae pathotypes in rice cropping center of Southeast Sulawesi using isogenic lines of IRRI, as well as to know the Xanthomonas oryzae pv. oryzae pathotypes dominating and spreading in rice cropping center of Southeast Sulawesi. The pathotype grouping was determined by leaf innoculated cutting method using 30 isolates of Xanthomonas oryzae pv. oryzae from Southeast Sulawesi in 10 isogenic lines, namely IRBB1, IRBB2, IRBB3, IRBB4, IRBB5, IRBB7, IRBB10, IRBB11, IRBB13, and IRBB21. Each isolate was inoculated on the 10 isogenic lines and repeated twice. Observation of disease intensity was conducted randomly on five leaves at four weeks after inoculation. The disease intensity was measured by the ratio of the length (cm) of leaf blight symptom to total the length of leaf sample (cm). The research results indicated there were seven pathotypes of Xanthomonas oryzae pv. oryzae in Southeast Sulawesi, i.e : pathotype X, XI, XII and four new pathotypes. Pathotype XII was the dominant pathotype, abort 79.31% of the total isolates and spreading in three regencies, Kolaka, Konawe, and South Konawe. Keywords: bacterial leaf blight, Xanthomonas oryzae pv. oryzae, pathotype

EVALUASI KESESUAIAN LAHAN TANAMAN KAKAO (Theobroma cacaoL.) BERDASARKAN ANALISIS DATA IKLIM MENGGUNAKAN APLIKASI SISTEM INFORMASI GEOGRAFI Safuan, La Ode; Kandari, Aminuddin Mane; Natsir, Muhammad
Jurnal Agroteknos Vol 3, No 2 (2013)
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The aims of the study were to determine the type of climate and the distribution and wide of each class of climate suitability for cocoa crop in the district of Buton. This study was conducted in March to August 2012 by using spline interpolation through the application of Geographic Information Systems (GIS) and ArcGIS 9.3 software and overlay method to get climatic units in the research are. The research found that climate type in the research area was climate type C (rather wet), subdistricts Batauga, Lasalimu, South Lasalimu, Siotapina and part region of including sub district Wolowa; and climate type D (medium) in the region of sub ditricts Kapontori, Wabula, Lapandewa and Sampolawa and part region sub district Wolowa, Lasalimu and Pasarwajo. Based on the results of evaluation of the suitability of the actual climate in the study area for the development of cocoa plants, there were nine (9 ) units of climatic suitability classes, namely S1, S2t, S2t, w1, S2w1, S3w1, S3w1, 3, S3w3, N1w3, and N2w1 with the area sizes of 91,16 ha or 0,08 %, 14.191,47 ha or 12,54 %, 10.726,04 ha or 9,47%, 2.245,34 ha or 1.98 %, 19.324,25 ha or 17,07 %, 17,80 ha or 0,02 %, 7.908,03 ha or 6,99 %, 29.017,40 ha or 25,63 % and 29.692,10 ha or 26,23 %. On the other hand, the potential climatic suitability classes were seven (7) units, namely S1, S2t, S2w1, S3w3, N1w1,3, N1w1 and N1w3 with each area size of 2.336,50 ha or 2,06%, 24.917,51 ha or 22,01%, 17,80 ha or 0,02%, 27.232,28 ha or 24,05%, 4.068,60 ha or 3,59%, 25.623,49 ha or 22,63% and 29.017,40 ha or 25,63% respectively, of the total evaluated area, with the limiting factors of temperature, climate, the number of dry months and moisture. This research showed that there are areas that can be developed for cocoa crop in the study area based on climate suitability. Keywords: climate, cocoa, land suitability

PERBANYAKAN BIBIT JERUK SECARA IN-VITRO DAN BEBAS PENYAKIT CVPD Wijayanto, Teguh; Taufik, Muhammad; Boer, Dirvamena; Suliartini, Ni Wayan Sri
Jurnal Agroteknos Vol 1, No 3 (2011)
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Abstract Citrus Vein Phloem Degeneration (CVPD) is one of the most important diseases of citrus. Worldwide and national yield losses of citrus production due to this disease infection have been very significant. This research ultimately aimed at producing citrus stocks through in-vitro culture, and free of CVPD. Citrus seeds were used as explant and cultured on MS and WPM in-vitro media, supplemented with malt extract. Citrus plantlets were tested for the presence of CVPD DNA using CVPD specific primers in PCR reactions. Research results showed that MS basal medium supplemented with malt extract was quite good for in- vitro production of citrus plantlets. Plantlets were negative for CVPD infection based on PCR tests. CVPD-free seedlings (Citrus reticulata) have been grafted with citrus rootstock (Citrus sinensis). Grafted citrus seedlings were also proven to be negative for CVPD infection based on similar PCR tests. Citrus seedlings/stocks produced by in-vitro culture, and free of CVPD, are now available for further growth. Keywords: Citrus Vein Phloem Degeneration (CVPD), in-vitro culture, PCR

PERTUMBUHAN DAN HASIL TANAMAN SAWI (Brassica juncea L.) PADA BERBAGAI DOSIS PUPUK UREA Erawan, Dedi; Yani, Wa Ode; Bahrun, Andi
Jurnal Agroteknos Vol 3, No 1 (2013)
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The aim of this research was to know the effect of urea fertilizer on growth and yield of mustard plant. This research was carried out at the Experimental farm of Agriculture Faculty, Haluoleo University, from October to November 2012. This research was carried out using completely randomized block design, that consisted of 6 treatments namely: without Urea fertilizer (U0), 25 kg ha-1 equals to 1.6 g plot1 of urea fertilizer (U25), 50 kg ha-1 equals to 3,2 g plot-1 of urea fertilizer (U50), 75 kg ha-1equals to 4,8 g plot-1of urea fertilizer (U75), 100 kg ha-1 equals to 6,4 g plot-1 of urea fertilizer (U100),125 kg ha-1 equals to 8 g plot-1 of urea fertilizer (U125) . Each treatment was repeated 3 times which resulted in 18 experiment units. The experiment data were analyzed using analysis of variance method and then followed by Honest Significantly Difference Test. The result of the research showed that the application of various dosages of urea fertilizer has an effect on the growth and yield of mustard plant. The application of urea fertilizer to mustard plant effected plant height, leaf area at the age of 21 and 28 days after planting, dry weight of root, dry weight of leaves and relative growth rate. However, the application of urea fertilizer has no effect on the number of leaves, leaf area at the age of 14 days after planting, leaf fresh weight, and the root fresh weight of mustard plant. An increase in the dosages of Urea fertilizer was followed by an increase in the growth and production of mustard plant. 125 kg ha-1 urea fertilizer increased growth and production of mustard plant a lot higher compared to other of urea fertilizer with a production yield of 10 ton ha-1. Keywords: mustard plant, urea

PENINGKATAN PERTUMBUHAN Pueraria javanica YANG DIINOKULASI FUNGI MIKORIZA ARBUSKULA DAN APLIKASI VERMIKOMPOS Arif, Asrianti
Jurnal Agroteknos Vol 1, No 1 (2011)
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The increase in plant productivity can be achieved by improving the quality of marginal lands (such as dried land, stone land, and mining lands). The efforts of land improvement include planting legume plant, application of organic fertilizer and introducing biological agent like Arbuscular Mycorrhiza Fungi (AMF). The combination of legume plant, AMF inoculation and vermicompost could be an effective method and need to be site tested. A pre-evaluation in finding the compatible AMF type to host and obtaining the best composition of vermicompost to Pueraria javanica growth has been conducted in a green house. This research was factorial experiment in CRD using two factors. The first factor was AMF inoculum type and the second factor was medium formulation. The growth factors measured were plant height, root dry weight, shoot dry weight, total dry weight and AMF propagule number. The research revealed that 20% AMF type of Glomus sp. combined with vermicompost composition treatment + 80% zeolit was the best treatment and capable of increasing root, shoot and total plant dry weights by 377%, 90%, and 704%, respectively, compared to the control. AMF inoculum type G. etunicatum (selected) and Glomus sp. (endogenous) gave different responses againts vermicompost addition in which type G. etunicatum was more tolerant than Glomus sp (endogenous). The best inoculum were Glomus sp, G.etunicatum and 30 % AMF type Glomus sp combined with vermicompost composition + 70% zeolit treatment, which produced propagule amount 10120 x 104, 308 x10, 77 x 104 per 100 g zeolit, respectively Key words : Pueraria javanica, Arbuscular Mycoriza fungi, vermikompost

PERKECAMBAHAN DAN PERTUMBUHAN TANAMAN SAWI (Brassica Juncea L.) YANG DIBERI PUPUK CAIR NUTRIFLORA DENGAN SISTEM HIDROPONIK Sarawa, Sarawa
Jurnal Agroteknos Vol 1, No 2 (2011)
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ABSTRACT The research was conducted to study seedling and growth of Brassica juncea applied with nutriflora liquid Fertilizer on hydrophonix system. The experiment has been carried out from September to December 2010 at Anduonohu, Haluoleo University Campus. The research was arranged in a randomized blocked design with three replications and five liquid fertilizer dosages; 2,5 ml L-1 water, 5,0 ml L-1 water, 7,5 ml L-1 water, 10 ml L-1 water, and 12,5 ml L-1 water. Characters observed were seedling percentage (%), vigor index, seedling dry weight (g), number of leaf, plant height (cm), fresh weight (g), shoot root ratio, and dry weight (g). The results of research indicated seedling and growth of Brassica juncea increased with application of nutriflora liquid fertilizer on hydrophonix system. The highest fresh weight of Brassica juncea occurred with the application of 7.5 ml L-1 water of nutriflora liquid ferlizer, and the highest dry weight of Brassica juncea occurred with the application10 ml L-1 water nutriflora liquid fertilizer. Nutriflora liquid fertilizer applied significantly correlated with the fresh and dry weights. Keywords: seedling, growth, Brassica juncea, liquid fertilizer

PENGARUH PEMBERIAN BERBAGAI TAKARAN SERASAH TANAMAN UBI KAYU TERHADAP PERTUMBUHAN BIBIT METE DAN KETAHANANNYA TERHADAP SERANGAN HAMA WERENG PUCUK METE (Sanurus indecora) DI PEMBIBITAN Nurmas, Andi; Mallarangeng, Rahayu; Mursalim, Soleha
Jurnal Agroteknos Vol 2, No 3 (2012)
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This research was conducted at the New Campus Anduonohu, Kendari from December 2011 to March 2012. The purpose of this study was to determine the effect of various doses of cassava plant litter on the growth of cashew seedlings and resistance to pest attack planthopper shoots cashew in the nurseries. The design of the study was Randomized Block Design (RBD) with 4 levels of treatment that were: treatment without cassava plant litter +(U0), the provision of cassava plant litter 40 g polybag-1, equivalent to 10 ton ha-1 (U1), the provision of cassava plant litter 80 g polybag-1, equivalent to 20 ton ha-1 (U2), the provision of cassava plant litter 120 g of polybag-1, equivalent to 30 ton ha-1 (U3) and each treatment was repeated 3 times so that there were 12 experimental units and each treatment used 2 cashew seedlings,therefore overall cashew seedlings used was 24 cashew seedlings. The results showed that administration of various doses of the cassava plant litter showed no significant effect on plant height, leaf number, stem diameter ages 60 and 90 days after planting, leaf area, dashed ratio of plant roots and dry weight and had significant effect on stem diameter age 30 days after planting. The intensity of hopper pests of cashew shoots (Sanurus indecora) obtained the highest treatment without cassava plant litter with the intensity of attacks and the lowest 13,9% obtained in the treatment of 10 ton ha-1, 20 ton ha-1 and 30 ton ha-1 with 2,8% intensity of attacks. Keywords: cassava plant litter, cashew seedlings, and Sanurus indecora.

KARAKTERISASI BIOKIMIAWI RIZOBAKTERI ASAL GULMA BERDAUN LEBAR YANG BERPOTENSI SEBAGAI DELETERIOUS RHIZOBACTERIA Asniah, Asniah; Rakian, Tresjia C.; Wangadi, Sri; Gusnawaty, HS
Jurnal Agroteknos Vol 3, No 3 (2013)
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The research aimed to know biochemical characters of rhizobacteria from broadleaf weed that are potential as deleterious rhizobacteria. The research was conducted at Agronomy Laboratory of Agriculture Faculty Halu Oleo University Kendari from January until March 2013. The results showed that 9 of 10 rhizobacteria isolates tested from broadleaf weeds rhizosphere had the ability to solubilize phosphate with different diameters. For nitrogen fixation ability, all isolates tested were potential but only isolates of ML-01 and KL-06 had high capability. All isolates had different ability to produce IAA, with isolates of KL-06 produced higher concentration of IAA (33,07 ppm) compared to other isolates. Isolates that had the ability to produce HCN were isolates BL-07, with filter paper change from yellow to dark brown, and BL-08 and BL-03 light brown color changes indicated to that the production of HCN was increased. Result of research showed that some isolates tested had biochemical character as deleterious rhizobacteria by the ability to solubilize phosphate, fix nitrogen, produce IAA and HCN. Keywords: biochemical characterization, rhizobacteria, broadleaf weeds, deleterious.

PENGARUH PUPUK KANDANG AYAM DAN NPK SEBAGAI PUPUK DASAR TERHADAP KESUBURAN TANAH DAN HASIL TANAMAN TOMAT PADA ULTISOL KAMBU KHUSNUL KHOTIMAH; SAHTA GINTING; FRANSISCUS S REMBON; DARWIS DARWIS; SYAMSU ALAM; NAMRIAH NAMRIAH
Jurnal Agroteknos Vol 13, No 2 (2023)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v13i2.43331

This study aims to determine the effect of chicken manure and NPK fertilizer as a basal fertilizer on Ultisol soil fertility and tomato plant yields and to find out the dose of chicken manure and NPK fertilizer as a basal fertilizer that affect both Ultisol soil fertility and tomato crop yields. This research was carried out at the Field Laboratory II of the Faculty of Agriculture and the Integrated Laboratory of Halu Oleo University, from March to June 2022. This study was conducted using a Randomized Block Design (RBD). The treatment tested was chicken manure and NPK fertilizer as a basal fertilizer consisting of 4 treatments, namely treatment without chicken manure (K0), chicken manure 200 g polybag-1 (K1), chicken manure 400 g polybag-1 (K2), chicken manure 600 g polybag-1 (K3). Each treatment was repeated by 3 tests, so there were 12 experimental units. The results showed that the application of chicken manure and NPK fertilizer as a basal fertilizer with various doses had an influence on soil fertility and tomato crop yields. In the K0 and K1 treatment, the fertility status was low, while the K2 and K3 treatment the fertility status increases to moderate, while the dose of chicken manure and NPK fertilizer as basal fertilizers that had higher on ultisol soil fertility and tomato plant yields, namely a dose of 600 g polybag-1 (K3) when compared to and a dose of 0 g polybag-1 (K0).

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