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alan H.E.A. Mokodompit, Kampus Baru, Jurusan Biologi Fakultas MIPA Universitas Halu Oleo Kendari
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BioWallacea Journal of Biological Research
Published by Universitas Halu Oleo
ISSN : 23556404     EISSN : 26856360     DOI : https://doi.org/10.1234/1234
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) telah memiliki ISSN 2355-6404 (print) dan ISSN 2685-6360 (online) yang merupakan salah satu jurnal nasional dan diterbitkan oleh Jurusan Biologi FMIPA Universitas Halu Oleo. Jurnal ini fokus pada ilmu biologi dan serumpun. Jurnal BioWallacea menerima naskah-naskah terbaik dari penulis yang bersifat asli hasil penelitian maupun telaah (review). Naskah ditulis dengan baik untuk setiap topik berkaitan dengan biologi yang berkembang saat ini serta bidang-bidang lain, termasuk: Penelitian Ekologi Fisiologi Ekofisiologi Taksonomi Botani Zoologi Mikrobiologi Biologi Laut Ilmu terapan berkaitan dengan ilmu Biologi seperti Bioteknologi, Biokimia, dan Biologi Sel serta Molekuler. Penelitian yang berkaitan isu lingkungan, termasuk Analisis Mengenai Dampak Lingkungan (AMDAL).
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol 2, No 1 (2015)" : 11 Documents clear
Keanekaragamaan dan Pemanfaatan Tumbuhan Obat Tradisional Pada Masyarakat di Kelurahan Lipu Kecamatan Betoambari Kota Baubau Provinsi Sulawesi Tenggara Indrawati Indrawati; Yusuf Sabilu; Puji Fitria Zainal
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 2, No 1 (2015)
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.201 KB) | DOI: 10.33772/biowallacea.v2i1.541

Abstract

The use of plants as traditional medicine has been known since long time ago by people in Lipu. This process has been inherited from one to next generation, nevertheless. nowdays, it has started to be extincted. So, it should be better to indentify the species of plant used as traditional medicine by people in Lipu. The research aims to investigate thekinds of plant which uses as traditional medicine. The part of plant used and how to process and the adventage of the plant. The research used explorative survey method. The result of the research there are 54 species from 31 family of plants used as traditional medicine by the people. The parts used of plant are leaf, stem/bark, root, flower, fruit, seed, tuber,rhizome and sap. The processing of the plants by refine, boil, squeeze, parch, pour boiling water, rasp and without processing. And the ability of the plants are can treat kinds of disease.
PENGHASILAN BIOPLASTIK OLEH ISOLAT INDIGENUS Bacillus sp. AMILOLITIK DENGAN SUBSTRAT PATI SUWEG (Amorphophallus campanulatus) Margino, Sebastian; Sari, Rarat Mulat; Martani, Erni
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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Abstract

bioplastic that was synthesized by bacteria. Glucose as the main carbon source for PHBproduction but its cost was expensive, so we need to substitute with another substrate,including starch.The ability of amylolytic bacteria hydrolyzed starch into simple sugars andconverted to PHB can minimize the costs of PHB production. This research aim was to findout amylolitic bacterial isolates and produce PHB using the selected isolate. A total of 52bacteria were isolated using starch mediumfrom soil sample around Amorphophalluscampanulatus plant. Twenty eight among of them had amylase activity, and eleven among ofthem had amylase activity value equal or more than 2 point.The next selection was donebased on the specific amylase activity and found that five of those isolates had specificamylase activity more than 10 DUN/mg.Final selection was done based on PHB productionand found that isolate E5 had the high amylase activity and PHB production. PHB productionused isolate E5 in optimum conditions (inoculum concentration 10 %, elephant foot yamstarch 3 %, medium pH 7, temperature 30 °C, agitation 125 rpm, and 48 hour incubationtimeshowed that percentage of PHB increased from 9,5% to 14,5%. Characterization andidentification showed that isolate E5 closed to the genus Bacillus sp.Keywords : Amylolytic Bacillus sp., poly-β-hidroxybutyrate, elephant foot yam starch.
KOMPOSISI DAN KEANEKARAGAMAN JENIS TUMBUHAN GOLONGAN POHON DI KAWASAN HUTAN LINDUNG NANGA-NANGA PAPALIA KOTA KENDARI SULAWESI TENGGARA Muhsin, Muhsin; Indrawati1, Indrawati; Rahardi, Wira
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
Publisher : Jurnal BioWallacea

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Abstract

The aim of this research was to know the composition, diversity and distribution ofdiameter class (vertical structures) of plant on tree categories in Protected Forest Area ofNanga-Nanga Papalia, Kendari Town, Southeast Sulawesi Province. This research wasdone by using 5 terraced transects, which was laid followed the river stream by consideringthe elevation place and vegetated condition. Each transect consist of 5 observed plots whichwere laid systematically along the transect line. Variables observed was the composition anddiversity of plant species by the growth stager including tree, pole, sapling, seedling andstand diameter. Ecological indicators of plants were analysed including number of species,density, relative density, frequency, relative frequency, dominancy, relative dominancy,important value index, diversity index, evennes index and stand diameter. The result showedthat were discovered about 53 species of plants on tree categories, which were classifiedinto 27 families and were distributed by the growth stager, i.e. 31 species of tree, 42 speciesof pole, 31 species of sapling and 26 species of seedling. Metrosideros petiolata had thehighest important value index for the growth stager tree and pole (54,474% and 62, 516%),while Baringtonia racemosa had the highest important value index for growth stager saplingand seedling (36,361% and 31,504%). The diversity index was in estimated grade for treeand seedling (2,908 and 2,529), while the diversity index was in high grade for pole andsapling (3,102 and 3,032). The distribution of stand diameter of plants in Protected ForestArea of Nanga-Nanga Papalia showed a balanced community which was dominated bytrees.Key words : Stand Diameter, Tree Categories, Protected Forest Area, Diversity,Composition.
STUDI KARAKTERISTIK MIKRO-HABITAT BURUNG MALEO (Macrocephalon maleo) PADA KAWASAN TAMAN NASIONAL RAWA AOPA WATUMOHAI (TNRAW) SULAWESI TENGGARA Jamili, Jamili; Analuddin, Analuddin; Rudia, Adi Parman Parman
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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Abstract

The purpose of this study was to determine the characteristics of the bird nestingmicrohabitat hole Maleo (Macrocephalon maleo) in National Parks Aopa WatumohaiSwamp, Southeast Sulawesi . Data were collected on the savanna region block MempahoForest and savanna region Pampaea , by using descriptive method to determine the depth,temperature, pH and substrate nesting holes were found during the study . To determine thecomposition of the vegetation around the nesting hole , using roaming with 10 meters toexplore the area around the hole nesting and record the type of vegetation found. Vegetationtypes have been known to direct scientific name recorded in the field. While the type ofvegetation that is unknown scientific name, a swab or documentation then be describedfurther in the Laboratory of Ecology and Natural Sciences Taxonomy Halu Oleo Universitywith reference to the book (FLORA by Steenis, et al., 1997). The results showed the depthof hole nesting birds Maleo (Macrocephalon maleo) in the study area is 50-60 cm,temperature 28o-32oC, and soil pH of 5.9 - 7. Maleo bird nesting substrate type is dominatedby sand. Types of vegetation found around the hole nesting nesting is Melastoma sp. andKirinyuh (Eupathorium sp.), while the type of vegetation that is a place to find food andshelter includes a thorn Bamboo (Bambusa spinosa), Rao (Dracontomelon mangiferum),Banyan (Ficus spp.), Tamarind (Aleurites molucana), Caesalpinia pulcherrima, forest Mango(Mangifera sp.), Kuia (Alstonia scolaris), Nona (Metrosideros petiolata), and Bitti/Kulipapo(Vitex sp.). Total current Maleo birds encounter is as much one of the males and femaleslaying eggs on the location of the savanna region Pampaea Resort Langkowala SwampNational Park area of Rawa Aopa Watumohai Southeast Sulawesi .Keywords : Characteristics microhabitat, Bird Maleo (Macrocephalon maleo), Rawa AopaWatumohai National Park Southeast Sulawesi.
KEANEKARAGAMAN JENIS SERANGGA PADA KOMUNITAS MANGROVE DI PULAU HOGA KABUPATEN WAKATOBI PROVINSI SULAWESI TENGGARA Suriana, Suriana; Jamili, Jamili; Rahman, Rahman
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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Abstract

The aims of this research were determine diversity and evenness index of insect atHoga Island Wakatoby Regency. This research used the mehtod of squared plot. It is wasmade 3 pieces purporsively line transect direct shoroline cut mangrove communities startfrom the most advanced (seaward) direction behind. Each transect plots observations weremade on the left and the right along the transects line. The diversity index and evennessindex was determined using Shanon-Wiener. The result showed that, there were 4 orders ofinsects which consist of 7 families and 13 genera. The highest diversity index (H’) of inscetswas found at Osbornia clodonata stand (0.25), while it was the lowest at Xilocarpusgranatums stand (0.14). Evenness index (E) of insect among has significance different all ofmangrove stands, namely a stand Bruguiera gymnorrizha (0.09), Pemphis accidula (0.07),Osbornia clodonata (0.12) and Xilocarpus granatum (0.07).Keywords : Diversity, Insects, Mangrove Community, Hoga Island
EFEK PEMBERIAN SARI BUAH PARIA (Momordica charantia, L.) TERHADAP MORFOLOGI SPERMATOZOA EPIDIDYMIS MENCIT (Mus musculus, L.) Harlis, Harlis; Malik, Nurhayu; Nelpiani, Nelpiani
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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The present research was conducted to study the efect Of Extract Fruit Paria(Momordica charantia, L.) On Morphology Spermatozoa Epididymis of mice (Mus musculusL.). The test animal used were 16 male mice with weight 20-30 gr aged 2-3 month into 4dosage treatments groups i.e. P0 control (water), P1 (25%/bw), P2 (50%/bw) and P3(75%/bw). The ectracts were given orally to all conducted during 34 day. At the 35th daybody weight mice be pondered the next surgery and conducted taking cauda epididymis forobservation morphology spermatozoa. The data obtained were analysed by using theAnalysis of Variance Test (ANOVA α = 0,05) and t-Test Least Significant Differences. Theresults showed the ectractsFruit Pariawere given on all dose reduce the percentage ofmorphology spermatozoa normal mice (Musmusculus, L.) is P1 (32%), P2 (47%), and P3(67%).Keywords : Ectracts Fruit Paria, Morphology Spermatozoa, Epididymis, Mice
KADAR N, P, DAN K KEDELAI (Glycine max (L) Merril) YANG DIAPLIKASI AZOTOBACTER sp., MIKORIZA DAN PUPUK ORGANIK Sabilu, Yusuf; Damhuri, Damhuri; Imran, Imran
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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Abstract

The purpose of this study was to determine levels of N, P, and K Soybean appliedAzotobacter sp. + Mycorrhizae and organic fertilizers. Type of experimental researchusing randomized block design (RAK) factorial design with two factors, namely thecombination of Azotobacter sp. + Mycorrhiza, consisting of 3 treatment that Azotobactersp. with a density of 103 CFU mL-1 + Mycorrhiza 30 grams, Azotobacter sp. with adensity of 104 CFU mL-1 + mycorrhiza 25 grams, Azotobacter sp. with a density of 105CFU mL-1 + Mycorrhiza 35 grams (K3); organic fertilizer factor consisted of 4 treatmentsie: without any organic fertilizer; organic fertilizer plot of 0.8 kg each equivalent to 2 tonha-1; 1.6 kg of organic fertilizer per plot equivalent to 4 ton ha-1, organic fertilizer plot of2.4 kg each equivalent to 6 ton ha-1. The results showed that the combination treatmentof Azotobacter sp. + Mycorrhiza there is not difference to the levels of nitrogen,phosphorus and potassium levels in the stem . Treatment of organic fertilizer there is asignificant difference on levels of nitrogen , phosphorus and potassium levels soybeanstem . No interaction combination of Azotobacter sp + Mycorrhiza and organic fertilizeron levels of nitrogen , phosphorus and potassium in soybean stems .Key words: Levels N , P , K Soybean, Azotobacter sp., Mycorrhizal, Organic Fertilizer.
Keanekaragamaan dan Pemanfaatan Tumbuhan Obat Tradisional Pada Masyarakat di Kelurahan Lipu Kecamatan Betoambari Kota Baubau Provinsi Sulawesi Tenggara Indrawati, Indrawati; Sabilu, Yusuf; Zainal, Puji Fitria
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
Publisher : Jurnal BioWallacea

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Abstract

The use of plants as traditional medicine has been known since long time ago bypeople in Lipu. This process has been inherited from one to next generation, nevertheless.nowdays, it has started to be extincted. So, it should be better to indentify the species ofplant used as traditional medicine by people in Lipu. The research aims to investigate thekinds of plant which uses as traditional medicine. The part of plant used and how to processand the adventage of the plant. The research used explorative survey method. The result ofthe research there are 54 species from 31 family of plants used as traditional medicine bythe people. The parts used of plant are leaf, stem/bark, root, flower, fruit, seed, tuber,rhizome and sap. The processing of the plants by refine, boil, squeeze, parch, pour boilingwater, rasp and without processing. And the ability of the plants are can treat kinds ofdisease.Keywords : Herbal medicine, Lipu Distric
KARAKTER TRIKOMA DAUN TANAMAN JATI (Tectona grandis L.) YANG DITANAM PADA TANAH PASCATAMBANG EMAS BOMBANA DENGAN VARIASI DOSIS PUPUK KANDANG KAMBING Ambardini, Sri; Indrawati, Indrawati; Ratnaen, Ratnaeni
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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The purpose of this study was to determine: the character trichomes of the plant teak(Tectona grandis L.) planted on gold post-mining soil Bombana with variation dose of goatmanure. This research is experimental use completely randomized design with 4 treatment,namely without goat manure (control/K0), and treatment of goat manure 100g 10kg-1 soil(K1), 125g 10kg-1 soil (K2), and 150g 10kg-1 soil (K3) in repeated 3 times. Teak plantparameters observed were the levels of length and number trichomes on the side abaksialand adaksial leaves (Tectona grandis L.). The results showed that goat manure of thetreatments K2 gives the highest value of length trichomes abaksial (995,46 μm), highestlength trikomes adaksial K0 (406,203μm). The biggest number trikomes abaksial on K3(3,481) and biggest number trichomes adaksial on K3 (4,047) too. Three character anatomyof trichomes was found in this research, namely dichotome multiselluler type on the abaksialleaf surface, slim uniselluler and biselluler type on the adaksial leaf surface.Key words: Teak (Tectona grandis L.), Goat manure, post-mining soil, trichomes.
SINTESIS ANTIGEN AFLATOKSIN M1-OVA ALBUMIN (Ova) SEBAGAI PEREAKSI AGAR GEL PRECIPITATION TEST (AGPT) Fusvita, Angriani
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
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Synthesis aflatoksin M1 -Ova albumin (Ova) antigen used as Agar Gel Precipitation Test(AGPT) reagent. The AFM1-Ova antigen was synthesized by reacting AFM1 standard andOva albumin using carboxymethoxylamine hemihydrochloride (CMO) as intermediatereactions. The component of Reagent were AFM1-Ova antigen and antibody G (Ig G/serum)used for AGPT. The result of AFM1-Ova Antigen synthesis were 3,45 mg/ml. AGPT resultshowed bond precipitation reaction beetween specific antibody with AFM1-Ova antigen.Therefore synthesis of AFM1-Ova antigen can be used to serology reagent test as AGPT.Keywords : Aflatoxin, Ova albumin, Aspergillus flavus, Antigen

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