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KARAKTER TRIKOMA DAUN TANAMAN JATI (Tectona grandis L.) YANG DITANAM PADA TANAH PASCATAMBANG EMAS BOMBANA DENGAN VARIASI DOSIS PUPUK KANDANG KAMBING Ambardini, Sri; Indrawati, Indrawati; Ratnaen, Ratnaeni
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
Publisher : Jurnal BioWallacea

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The purpose of this study was to determine: the character trichomes of the plant teak(Tectona grandis L.) planted on gold post-mining soil Bombana with variation dose of goatmanure. This research is experimental use completely randomized design with 4 treatment,namely without goat manure (control/K0), and treatment of goat manure 100g 10kg-1 soil(K1), 125g 10kg-1 soil (K2), and 150g 10kg-1 soil (K3) in repeated 3 times. Teak plantparameters observed were the levels of length and number trichomes on the side abaksialand adaksial leaves (Tectona grandis L.). The results showed that goat manure of thetreatments K2 gives the highest value of length trichomes abaksial (995,46 Î¼m), highestlength trikomes adaksial K0 (406,203Î¼m). The biggest number trikomes abaksial on K3(3,481) and biggest number trichomes adaksial on K3 (4,047) too. Three character anatomyof trichomes was found in this research, namely dichotome multiselluler type on the abaksialleaf surface, slim uniselluler and biselluler type on the adaksial leaf surface.Key words: Teak (Tectona grandis L.), Goat manure, post-mining soil, trichomes.

Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste Yanti, Nur Arfa; Ahmad, Sitti Wirdhana; Ambardini, Sri; Muhiddin, Nurhayani Haji; Sulaiman, La Ode Iman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10241

Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production.

Pertumbuhan dan Alokasi Biomassa Organ Tanaman Mahoni (Swietenia mahagoni L.) yang Ditanam Pada Tanah Bekas Tambang Emas dengan Perlakuan Pupuk Kandang Ambardini, Sri; Ningsih, Rita; Kali, Yustina Rante
bionature Vol 19, No 1 (2018): April
Publisher : Fakultas MIPA UNM

Abstract. Â The purpose of this study is to determine the growth and biomass allocation of Mahagony plant organs are planted on ex-gold mining soil with manure treatment. The experimental research with four treatments are control (without manure), manure (cow, goat, chicken) with five replications. The plant growth parameters are observed consist of plant height, stem diameter, leaf number, leaf area, rooth lenght, dry weight, and biomass percentage are located to leaves, stems and plant roots. The data are tested anova and continue with t-test. The result showed that the treatment of manure had efeect on the growth and mahagony planted on ex-golg mining soil. Plant biomass on all manure treatment are largest alloccated to leaves organ then to stems and roots. Based on t-test result that the cow manure treatment is better to supporting growth and biomass of mahagony plant than chicken manure, goat manure and control.Keywords: growth, biomass, mahagony, ex-gold mining soil, manure

Biomassa Bibit Tanaman Jambu Mete (Anacardium occidentale L.) yang Ditanam pada Tanah Pascatambang Emas Bombana dengan Variasi Pupuk Kandang Ambardini, Sri
Al-Kauniyah: Jurnal Biologi Vol 7, No 2 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Research to back up ex gold miningÂ restoration must Â been done with Â productive plant so gets to answer about problem environmental one arises mining industry effect and can get to increase economy of plant exploit facet. This research aims was to know plant seed growth Cashew nut (Anacardium occidentale L.) with biomassa accumulation on organ comes to root, bar, and plant seed leaf of Cashew nut ( Anacardium occidentale L.) that old three-month after implant. Experiment research utilize fledged random design (RAL), consisting tree manures (crib manures, goat den manures, and henhouse manures) with same dose, which is 150 ha-1 tons, and control (without manures) with five replicated. Experimental procedure consisting of plant media preparation utilize ex mining land, preparation organic and an organic manure seed instilling, manuring, preserve, and cropping. Instilling and plant preserve is done in green house FMIPA UHO'S Biological. Result observationaling to point out that biomassa allocation plant tends to be presented at by leaf then bar organ and root, well on control and also on conduct by manures den. Biomassa allocation percentage in plant organ that is given manures greater crib than deep plant organ on conduct manures henhouse, goat den manure and control. Biomassa allocation most little on organ comes to root with percentage most little on conduct manures crib then on henhouse manure and supreme on goat den manure.

ISOLASI DAN IDENTIFIKASI KAPANG PELARUT PHOSPHATE DARI RIZOSFER GADUNG (DIOSCOREA HISPIDA DENNST) DAN BAMBU (DENDROCALAMUS ASPER) Suleman, Darwis; Sani, Asrul; Ambardini, Sri; Yanti, Nur Arfa; Boer, Dirvamena
Berkala Penelitian Agronomi Vol 7, No 2 (2019)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/bpa.v7i2.10564

Fosfor (P) merupakan hara utama kedua yang membatasi produksi tanaman, namun ketersediaannya di dalam tanah sangat masalah karena reaksi presipitasi dengan Al+3 dan Fe+3 pada tanah masam atau Ca+2 pada tanah alkalin. Selama beberapa tahun terakhir, pemanfaatan mikroba tanah di anggap sebagai salah satu alternatif untuk meningkatkan ketersediaan P untuk tanaman. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang pelarut fosfat dari rizosfer bambu dan gadung. Isolasi kapang pelarut fosfat dilakukan pada kondisi in vitro, melalui serangkaian pengenceran dengan menggunakan media Pikovskaya padat. Hasil penelitian diperoleh dua isolat dari rizosfer gadung dan satu isolat dari rizosfer bambu. Namun, hasil uji kelarutan P hanya satu isolat dari gadung yang menunjukkan zona bening di sekitar koloni. Secara kualitatif, kelarutan P bervariasi antara 2,405 dan 2,052 untuk gadung dan bamboo. Analisis numerik-fenetik mengungkapkan bahwa isolat kapang dari gadung terdeteksi sebagai Talaromyces aculeatus dengan indeks kesamaan 100% dan isolat kapang dari bambu teridentifikasi sebagai Fuzarium proliferatum dengan indeks kesamaan 89,7% dibandingkan dengan kapang acuan.Kata kunci : Asam organik, kapang, phosphate, pelarut, rizosfer

PERTUMBUHAN DAN DAYA SERAP Chlorella vulgaris TERHADAP LOGAM BERAT TIMBAL (Pb) PADA PERAIRAN DALAM SKALA LABORATORIUM Anggraini, Wa Ode Linda Rahayu; Iba, Wa; Ambardini, Sri
JSIPi (Jurnal Sains dan Inovasi Perikanan) (Journal of Fishery Science and Innovation) Vol 6, No 1 (2022): JURNAL SAINS DAN INOVASI PERIKANAN
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jsipi.v6i1.14992

This research aims to determine the growth of C. vulgaris (based on specific growth rate and biomass productivity) cultured in Walne medium containing different concentration of lead.Â C. vulgaris ability in absorbing heavy metals Pb was also measured. This study used a complete randomized design experiment with walne media and the addition of varied concentration of lead.Â Four lead concentrations used were 3, 5, 7 and 9 ppm. Microalgae was cultured for 8 days at 30 psu and 12:12 photoperiod. Increasing density was calculated every other day. Microalgae was harvested by using 1 M NaOH solution to obtain cell biomass. The lead absporption of C.vulgaris was read using an atomic absorption spectrophotometer. Cell density, specific growth rate and biomass productivity were the highest at 3 ppm and the lowest at 9 ppm. Better lead absorption of C. vulgaris cells was observed at 5 ppm than other concentration tested. This research confirmed that C. vulgaris can be used as bioremediation agent to lead contamination in sea water up to concentration of 9 ppm.

ANALISIS SOAL UJIAN NASIONAL IPA BERDASARKAN DIMENSI PROSES KOGNITIF TAHUN 2016-2018 Sinta, Wa; Fahyuddin, Fahyuddin; Ambardini, Sri
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Publisher : Halu Oleo University

Penelitian ini bertujuan untuk menganalisis penyebaran soal Ujian Nasional IPA tahun 2016 - 2018 berdasarkandimensi proseskognitif. Jenis penelitian ini adalah penelitian deskriptif.Sampel dalam penelitian ini adalah peserta didik kelas IX SMP Negeri di Kabupaten Wakatobi yang telah mengikuti Ujian Nasional tahun 2016-2018. Sampel penelitian ini diambil dengan metode stratified random sampling. Data penelitian diperoleh melalui observasi. Hasil analisis data menunjukkan: 1) distribusi persentase tingkat kognitif C1 soal Ujian Nasional tahun 2016 ada 20% menurun menjadi 15% ditahun 2017 dan 10% ditahun 2018; 2)persentase tingkat kognitif C2 soal Ujian Nasional tahun 2016 ada 25% meningkat menjadi 40 % ditahun 2017 dan 45% ditahun 2018; 3) persentase tingkat kognitif C3 soal Ujian Nasional tahun 2016 ada 50% menurun menjadi 37,5 % ditahun 2017 dan 35% ditahun 2018, dan 4) persentase tingkat kognitif C4 soal Ujian Nasional tahun 2016 ada 5% meningkat menjadi 7,5 % ditahun 2017 dan 10% ditahun 2018 dari total 40 nomor soal Ujian Nasional materi IPA.Kata Kunci: Ujian Nasional, Dimensi Proses Kognitif

Peranan Jamur Rhizoctonia sp. Asal Taman Nasional Rawa Aopa Watumohai Sulawesi Tenggara terhadap Keberhasilan Aklimatisasi dan Laju Pertumbuhan Planlet Anggrek Macan (Grammatophyllum scriptum BL.) Rita Ningsih; Sri Ambardini; Denofia Denofia
Al-Kauniyah: Jurnal Biologi Vol 7, No 2 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

The aim of this research was to know the role of Rhizoctonia sp. fungus from Taman Nasional Rawa Aopa Watumohai (TNRAW) to the successfully acclimatization and growth rate of tiger orchid planlet (Grammatophyllum scriptum Bl.) as well to know successfully infection of fungus Rhizoctonia sp. at the planlet root. The procedure of this research consists 3 main steps, they were fungus inoculation on orchid’s growing media, growth test and verification of successfully infection on planlet root. Growth test consisting of 3 treatments namely control (without nutrition and fungi) (K0); Rhizoctonia sp. application (R1); Rhizoctonia sp. + nutrition Potato Dextrose Borth (PDB) (R2) with the repetition for 8 times, then the growth data was analysed by statistics utilizes to F-test and continue by BNT-test on 95% trusty level. The result of the research showed that R2 treatment increase high planlet, leaf and root number. All the mean score of research indicator R2 was highest compared R1 and K0. The result of F-test showed R1 and R2 treatment gave significant influence toward the high planlet, leaf and root number growth. Except, fresh and dry weight planlet. Eventhough, fresh and dry weight was higher compared than K0 acclimatization period for a month. Rhizoctonia sp. fungus infected planlet root through velamen to eksodermis and cortex by forming hifa scroll (pelotons).

Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste Nur Arfa Yanti; Sitti Wirdhana Ahmad; Sri Ambardini; Nurhayani Haji Muhiddin; La Ode Iman Sulaiman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10241

Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production.

Aktivitas Antibakteri Kombucha Daun Sirsak (Annona muricata L.) Dengan Konsentrasi Gula Berbeda Yanti, Nur Arfa; Ambardini, Sri; Ardiansyah, Ardiansyah; Marlina, Wa Ode Leni; Cahyanti, Kartika Dwi
BERKALA SAINSTEK Vol 8 No 2 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i2.15968

Kombucha daun sirsak merupakan minuman hasil fermentasi simbiosis antara bakteri asam asetat Acetobacter xylinum dan khamir Saccharomyces sp. yang menggunakan bahan baku rebusan daun sirsak. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kombucha daun sirsak dengan variasi konsentrasi gula yang berbeda. Konsentrasi gula yang digunakan dalam pembuatan kombucha daun sirsak adalah 10%, 20%, 30% dan 40% (b/v). Pengujian aktivitas antibakteri dilakukan dengan metode difusi sumuran menggunakan bakteri Escherichia coli dan Staphylococcus aureus. Kombucha daun sirsak memiliki aktivitas antibakteri dengan spektrum luas. Kombucha daun sirsak dengan konsentrasi gula 20% memiliki aktivitas antibakteri tertinggi. Dengan demikian, kombucha daun sirsak sangat berpotensi sebagai minuman kesehatan.

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