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Suriana
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alan H.E.A. Mokodompit, Kampus Baru, Jurusan Biologi Fakultas MIPA Universitas Halu Oleo Kendari
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BioWallacea Journal of Biological Research
Published by Universitas Halu Oleo
ISSN : 23556404     EISSN : 26856360     DOI : https://doi.org/10.1234/1234
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) telah memiliki ISSN 2355-6404 (print) dan ISSN 2685-6360 (online) yang merupakan salah satu jurnal nasional dan diterbitkan oleh Jurusan Biologi FMIPA Universitas Halu Oleo. Jurnal ini fokus pada ilmu biologi dan serumpun. Jurnal BioWallacea menerima naskah-naskah terbaik dari penulis yang bersifat asli hasil penelitian maupun telaah (review). Naskah ditulis dengan baik untuk setiap topik berkaitan dengan biologi yang berkembang saat ini serta bidang-bidang lain, termasuk: Penelitian Ekologi Fisiologi Ekofisiologi Taksonomi Botani Zoologi Mikrobiologi Biologi Laut Ilmu terapan berkaitan dengan ilmu Biologi seperti Bioteknologi, Biokimia, dan Biologi Sel serta Molekuler. Penelitian yang berkaitan isu lingkungan, termasuk Analisis Mengenai Dampak Lingkungan (AMDAL).
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 157 Documents
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Optimasi Ekstrinsik Produksi Protease dari Bacillus sp. TG-50 Isolat Tanah Tempat Pembuangan Akhir Sampah (TPAS) Talang Gulo Jambi Hafiz Muchti Kurniawan; Rizka Fikrinnisa; Ade Adriadi
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 1 (2022): BioWallacea Universitas Halu Oleo
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.51 KB) | DOI: 10.33772/biowallacea.v9i1.24584

Abstract

Protease adalah enzim yang menghidrolisis ikatan peptida pada molekul protein yang memiliki potensi yang besar untuk diterapkan dalam bidang industri, terutama industri deterjen, produk susu, minuman, farmasi, kue dan penyamakan kulit. Penelitian ini mengkaji optimisasi lingkungan ekstrinsik produksi enzim protease dari Bacillus sp. TG-50. Penelitian ini menggunakan metode eksperimental yang terdiri dari optimasi suhu dan pH medium untuk memproduksi protease dan optimasi sumber karbon terhadap variasi konsentrasi dan sumber nitrogen anroganik terhadap variasi rasio C/N. Hasil pengamatan kurva pertumbuhan Bacillus sp. TG-50 diatas diketahui bahwa protease dihasilkan pada jam ke-2 setelah inkubasi dan meningkat pada fase eksponensial pada jam ke-12 dan mulai menurun pada fase stasioner dan fase kematian, hal ini menunjukan bahwa enzim protease yang dihasilkan Bacillus sp. TG-50 tergolong kedalam metabolit primer. Hasil perhitungan terhadap kinetika pertumbuhan Bacillus TG-50 menunjukkan bahwa konstanta laju pertumbuhan µ = 0,173 jam-1. pH 7 merupakan pH optimal dalam meningkatkan laju aktivitas spesifik protease dengan nilai 1,294 U/mg. Hasil pengukuran terhadap aktivitas spesifik protease pada variasi suhu pada pH 7 menunjukkan bahwa semua variasi suhu memberikan pengaruh terhadap laju aktivitas spesifik protease. Suhu optimal ditunjukkan pada suhu 35 0C pada waktu inkubasi 48 Jam dengan nilai 4,52 U/mg. Hasil pengukuran aktivitas spesifik protease pada sumber karbon yang berbeda dalam konsentrasi berbeda pada suhu 35 0C dan pH 7 menunjukkan bahwa Maltosa 1,5% memberikan hasil paling tinggi yakni 5,005 U/mg. sedangkan Maltosa 0,5% memberikan hasil pengukuran paling kecil dibandingkan sumber karbon yang lain yakni sebesar 0,832 U/mg. pemberian sumber nitrogen KNO3 dengan rasio C/N=10 yang diinkubasi pada suhu 35 0C dan pH 7 memperlihatkan hasil aktivitas spesifik protease paling tinggi yaitu sebesar 6,034 U/mg. pemberian rasio C/N=20 memperlihatkan hasil pengukuran aktivitas spesifik protease paling rendah yaitu sebesar 2,073 U/mg dan pemberian (NH4)2SO4 menunjukkan aktivitas spesifik protease terkecil yakni sebesar 2,203 U/mg. 
Keanekaragaman Makrozoobentos sebagai Bioindikator Lingkungan di Sungai Lantahiwo Desa Kotawo Kecamatan Kulisusu Barat Kabupaten Buton Utara La Baco Sudia; Kahirun Kahirun; La Ode Muhammad Erif; Abdul Manan; Surya Cipta Ramadhan Kete; Ruslin Ruslin
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 10, No 1 (2023): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v10i1.38410

Abstract

Macrozoobentos are animals that live permanently in sediments at the bottom of the water, both on soft and hard substrate conditions. Macrozoobenthos contribute greatly to the function of aquatic ecosystems and play important roles such as mineralization processes in sediments and organic material cycles and play a role in transferring energy through the food chain. Based on the results of research conducted on the Lantahiwo River, Kotawo Village, West Kulisusu District, the species richness of macrozoobenthos found in the Lantahiwo River was varied where the number of individuals obtained from station 1 (upstream) was 20 individuals. At station 2 (middle of the river) there were 60 individuals, while at station 3 (downstream) there were 14 individuals. The river is a form of aquatic ecosystem that has an important role in the hydrological cycle and functions as a catchment area for the surrounding area. So that the condition of a river is strongly influenced by the characteristics of the surrounding environment. The sampling point includes looking at the considerations made in the field and sampling at stations 1, 2 and 3 is carried out in stages, first in the upstream, middle and downstream parts of the river then observations are made with several parameters that are considered important by researchers as key parameters that represent and describes the waters of the Lantahiwo River. This research was conducted from March to April 2020 in Kotawo Village, Kulisusu Barat District, North Buton Regency. Based on the results of identification, where the types of macrozoobenthos found in the Lantahiwo River at stations 1 to 3 consist of 2 families and 2 classes, namely; types of family Gerridae from class Bivalvia, species from family Thiaridae from class Gastropods, species from family Thiaridae from class Macrozoobentos were found at each station.
Pertumbuhan Dan Adaptasi Biokimia Mangrove Rhizophora apiculata Blume., Pada Media Dengan Kondisi pH (Derajat Keasaman) yang Berbeda
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 10, No 1 (2023): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v10i1.29448

Abstract

The purpose of this study was to determine the effect of pH (acidity) on the growth of Rhizophora apiculata Blume mangroves. to determine biochemical adaptation (vitamin C, anthocyanins and tannins). This research was an experimental research. The number of samples set was 9 mangrove seedlings. The data were analyzed by using Kaleidagraph software as well as Ms. Excel software to obtain pH (acidity) relationships between controls and treatments. The results showed that the effect of pH (acidity) obtained the highest average of relative growth rate to plant height, about 0.00475, stem diameter about 0.0147 and leaf area about 0.045; The highest biochemical adaptation of vitamin C, anthocyanins.
Polisi dan Polusi (Studi Kasus Kapasitas Paru Polisi Lalu Lintas Polres Kendari)
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 10, No 1 (2023): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v10i1.24362

Abstract

Polantas (Traffic Police) is part of the Police that is needed by the community for security, especially traffic on the highway. The increase in the number of motorized vehicles has an impact other than traffic congestion can also trigger air pollution caused by motor vehicle emissions, this can have a negative impact on the lungs of traffic police. The purpose of this study was to obtain data on air quality in Kendari City and vital lung capacity of the Kendari Police Traffic Police. Measurement of lung capacity using a spirometry tool. The measurement of air quality uses the IMR 1600 tool. Measurements are carried out for at least 1 hour and then recorded in the form of a report. Parameters that can be detected by this tool: Carbon Monoxide (CO), Nitrogen Dioxide (NO2), and sulfur dioxide (SO2 ). The results showed that the vital capacity of the lungs of traffic police at the Kendari City Police consisted of three categories, namely normal, mild disturbances and moderate disturbances, while concentrations of sulfur dioxide (SO2), carbon monoxide (CO) and nitrogen dioxide (NO2) in the air, not exceeding the air quality standard. The results of the calculation of the correlation test show that there is no relationship between the air quality of both Sulfur Dioxide (SO2), Carbon monoxide (CO) and Nitrogen dioxide (NO2 ) with the vital quality of the lungs at the Traffic Police on duty at the Kendari Police.Keywords: Pollution, Lungs, Air Quality
Komposisi dan Keanekaragaman Tumbuhan Rawa Air Tawar Musiman Kasiono Oe Kecamatan Lawa Kabupaten Muna Barat Sulawesi Tenggara
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 10, No 1 (2023): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v10i1.40397

Abstract

This reserch purpose is to determine the composition and diversity of seasonal freshwater swamp plant growth of Kasiono Oe, Lawa District, West Muna Regency, Southeast Sulawesi and to determine environmental factors that affect the growth of seasonal freshwater swamp plant Kasiono Oe, Lawa District, West Muna Regency, Southeast Sulawesi.This research is a descriptive-quantitative research conducted at 5 stations in the swamp area, namely station I (Lianoghule river flow), station II (Mata river flow), station III (Soni river flow), station IV (close to settlements) and station V (near residential area). with plantations). Determination of the location using the line method and plotted lines, the determination of the line method with a width of 20x20 (for the tree phase) and the line method (for the seedling, sapling and tihang phases) installation of a 50 m transect with a length of 100 m.The results showed that the composition of plant species found in station I was found 4 plant species totaling 115 individuals, station II found 9 plant species totaling 71 individuals, station III found 5 plant species totaling 49 individuals, station IV found 5 plant species totaling 61 individuals and station IV found 8 plant species totaling 109 individuals. The diversity index for tree strata was 1.03, tihang strata 0.63, sapling strata 1.3 and seedling strata at station II 1.09 and station V 1.01. The highest uniformity index for the tree strata was 0.943 and the lowest was 0.918, for the tihang strata 0.91, the highest sapling was 0.97 and the lowest was 0.81 and the seedling strata had the highest 0.Keywords: Composition of plant species, diversity, Seasonal freshwater swamp.
Jenis-Jenis Lamun (Seagrass) Di Perairan Pantai Negeri Hutumuri Kecamatan Leitimur Selatan Kota Ambon
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 10, No 1 (2023): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v10i1.35048

Abstract

Seagrass is a very important plant that grows in coastal areas. The beach of Hutumuri village which is bordered by the open sea can affect the existence of seagrass beds and there are housing along the coast so that it allows for community activities on the beach. The purpose of this study was to determine the types of seagrass in the coastal waters of Hutumuri Village, Ambon City. The data collection method used was the cruising method which was carried out to obtain the types of seagrass at the research location. Sampling of seagrass was carried out at maximum low tide in November 2022. In this study, 3 types of seagrass were found, namely Thalassia hemprichii, Halodule pinifolia and Cymodocea rotundata. Thalassia hemprichii has flat leaves, longitudinal leaves like lines, there are brown lines on the leaves, leaf blades are slightly curved, 5-20 cm long leaves, 4 cm wide leaves. The tips of the leaves are rough and rounded. Each stand has 3-4 leaves. Each node contain 1 root surrounded by small dense hairs. Halodule pinifolia leaves are flat, narrow like needles, leaf tips are rounded. Leaf blade length 6-15 cm, leaf blade width 0.1-0.2 cm. Each stand has 1-2 leaves. each node has a number of small roots, the distance between nodes 1-3 cm. Cymodocea rotundata has flat leaves, leaf blade length 7-15 cm, blade width 2-4 cm. Each stand has 2-4 leaves. Between rhizomes there are nodes. Each stand and node has 1-2 roots. The distance between nodes is 2 cm.
Upaya Pengelolaan Ekosistem Mangrove Berkelanjutan di Teluk Kendari Provinsi Sulawesi Tenggara
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 10, No 1 (2023): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v10i1.40407

Abstract

One of the threats posed by community and local government in the coastal area of Kendari Bay was the management of mangrove ecosystems that is not friendly, which was ignores applicable environmental laws and regulations. Aims of this research was to analyze the composition and diversity of mangrove species, the level of mangrove deforestation and degradation, and to formulate strategies for sustainable mangrove ecosystem management efforts in Kendari Bay, Southeast Sulawesi. Some of the analyzes in this research was: remote sensing system with guided classification method, vegetation analysis using line transect and plot method, description and SWOT analysis. The results showed that changes in the area of mangrove forests that occurred in 2014 to 2017 decreased by 2 ha, while from 2017 to 2020 there was an increase of 26 ha. Mangrove degradation level of Kendari Bay in 2014 to 2020 anlyzed by standard criteria for mangrove degradation of Ministry of Environment and Forestry Decree No. 201 of 2004 is categorized as damaged with tree density / ha very rare. The results of the vegetation analysis showed that were 11 mangrove species in the 5 observation stations of Kendari Bay. Rhizophora mucronata is the dominant species in the Kendari Bay mangrove community from 5 observation stations. Mngrove degradation caused by factors of economic, low of education and skills, and lack of control from the authorities. Based on the SWOT diagram and matrix, the position of the mangrove management strategy at the research location is in the third quadrant (Turn Around strategy).Key words: Upaya pengelolaan; tingkat kerusakan; mangrove.

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