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BioWallacea Journal of Biological Research
Published by Universitas Halu Oleo
ISSN : 23556404     EISSN : 26856360     DOI : https://doi.org/10.1234/1234
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) telah memiliki ISSN 2355-6404 (print) dan ISSN 2685-6360 (online) yang merupakan salah satu jurnal nasional dan diterbitkan oleh Jurusan Biologi FMIPA Universitas Halu Oleo. Jurnal ini fokus pada ilmu biologi dan serumpun. Jurnal BioWallacea menerima naskah-naskah terbaik dari penulis yang bersifat asli hasil penelitian maupun telaah (review). Naskah ditulis dengan baik untuk setiap topik berkaitan dengan biologi yang berkembang saat ini serta bidang-bidang lain, termasuk: Penelitian Ekologi Fisiologi Ekofisiologi Taksonomi Botani Zoologi Mikrobiologi Biologi Laut Ilmu terapan berkaitan dengan ilmu Biologi seperti Bioteknologi, Biokimia, dan Biologi Sel serta Molekuler. Penelitian yang berkaitan isu lingkungan, termasuk Analisis Mengenai Dampak Lingkungan (AMDAL).
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 157 Documents
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Prospek Pemanfaatan Limbah Pertanian Sebagai Pupuk Organik Cair (POC) Pada Budidaya Tanaman Sawi (Brassica juncea L.) Dalam Pot Robiatul Adawiyah; Terry Pakki; Namriah Namriah; Agung Yuswana
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.163 KB) | DOI: 10.33772/biowallacea.v9i2.28721

Abstract

This study aims to determine the prospects for utilization of agricultural waste in the cultivation of mustard greens (Brassica juncea L.) in pots. This research was conducted in the Padaleu Village, Kambu District, Kendari City and the Laboratory ofthe Agronomy Unit of the Faculty of Agriculture, Halu Oleo University. The research was carried out from September to November 2018. The design used in this study was a one-factor randomized block design (RDB), namely the type of liquid organic fertilizer (LOF) various agricultural wastes consisting of control (P0), LOF of jackfruit waste (P1), LOF of banana weevil (P2),and LOF of Moringaleaves (P3).The LOF was given with the same concentration, namely15ml L-1 of water. Each treatment was repeated 3 times in order to obtain 12 experimental units and each experimental unit consistedof 3 plants for a total of 36 plants. The variables observed were plant height (cm), number of leaves (strands), leaf length (cm), leaf width (cm), wet weigh tand dry weight of mustard plant stover (g), wet weight and dry weight of mustard roots (g). There sults showed that the LOF treatment of various types of agricultural waste had a significant effect on all the observed variables. The effect of jackfruit waste LOF, banana weevil LOF and Moringa leaf LOF was the same effective for all observed variables, but significantly different from treatment without LOF (control). Therefore, the three typesof LOF are very prospective to be used as LOF to reduce the use of inorganicfertilizers. Key words: Agricultural waste, mustard greens, liquid organic fertilizer. Abstrak Penelitian ini bertujuan untuk mengetahui prospek pemanfaatan limbah pertanian pada budidaya tanaman sawi (Brassica juncea L.) dalam pot. Penelitian ini dilaksanakan d iKelurahan Padaleu Kecamatan Kambu Kota Kendari dan Laboratorium Unit Agronomi Fakultas Pertanian Universitas Halu Oleo. Pelaksanaan penelitian berlangsung dari Bulan September sampai Bulan November 2018.  Rancangan yang digunakan dalam penelitian ini adalah rancangan acak kelompok(RAK) satu faktor yaitu jenis pupuk organik cair (POC) berbagai limbah pertanian terdiri atas control (P0), POC limbah nangka (P1), POC bonggol pisang (P2),dan POC daun kelor (P3).  Pemberian POC tersebut dengan konsentrasi yang sama yaitu 15 ml L-1 air. Setiap perlakuan diulang 3 kali sehingga diperoleh 12 unit percobaan dan setiap unit percobaan terdiri dari 3 tanaman sehingga total 36 tanaman. Variabel yang diamati yaitu tinggi tanaman (cm), jumlah daun (helai),panjang daun (cm),lebar daun (cm), bobot basah dan bobot kering brangkasan tanaman sawi (g), bobot basah dan bobot kering akar tanaman sawi (g). Hasil penelitian menunjukkan bahwa perlakuan POC berbagai jenis limbah pertanian berpengaruh nyata terhadap semua variable lpengamatan. Pengaruh pemberian antara POC limbah nangka, POC bonggol pisang dan POC daun kelor sama terhadap semua variabel pengamatan, namun berbeda nyata dengan perlakuan tanpa POC (kontrol). Oleh karena itu ketiga jenis POC tersebut sangat prospek dijadikan sebagai POC untuk mengurangi penggunaan pupuk anorganik. Kata kunci: Limbah pertanian,tanaman sawi, pupuk organik cair.
Tinjauan Pemanfaatan Citra Sentinel dan Machine Learning Dalam Pendugaan Volume Tegakan Hutan Zulkarnain Zulkarnain; Muhammad Buce Saleh
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1043.874 KB) | DOI: 10.33772/biowallacea.v9i2.26227

Abstract

This literature review is carried out systematically and aims to identify and analyze research trends, data collections, methods, and frameworks used in estimating forest stand volume (VTH) research with sentinel and (machine learning) ML from 2014 to 2021 so that it can provide answers. on the research questions of this study. The results of the analysis of 24 selected articles based on inclusion-exclusion criteria showed that VTH studies with sentinel and ML generally combine sentinel with other images, such as ALOS-2 L Band, Landsat, ALOS DSM and DEM data. S2-spectral band, S2-vegetation index, slope, S1-backscatter, elevation, are the variables most widely used as predictors. RF (random forest regression), SVR (support vector regression), MLR (multi linear regression) and kNN (k-nearest neighbor) algorithms are the most widely used algorithms. The potential for significantly increasing the accuracy of VTH estimation results can be done by adding environmental factors as a predictor variable.The study of Mauya et al, Reis et al and Chen et al which combines optical, SAR and topographic data, reported a significant increase in the accuracy of VTH estimation.Furthermore Mauya et al suggested that the weighted average approach of pixels in extracting variables from image based on the position of the field plot produces a better estimation model than using the centroid approach Optimizing the use of red edge bands in VTH estimation conducted by Jiang et al, Ahmadi et al and Hu et al, shows a more significant correlation between red edge bands and VTH than other S2-derived features s proves that the modification of the vegetation index formula by replacing the band (NIR) with a red edge band is proven to significantly increase its correlation with stand volume and performs very well on the RF, SVM and MLR algorithms in estimating VTH. The kriging geostatistical method by Chen et al and Bolat concluded that SVR-kriging and Regression-kriging each outperformed SVR and had better accuracy in predicting VTH. AbstrakTinjauan literatur ini dilakukan secara sistematis dan bertujuan untuk mengidentifikasi serta menganalisis tren penelitian, kumpulan data, metode, dan kerangka kerja yang digunakan dalam penelitian pendugaan volume tegakan hutan (VTH) dengan sentinel dan machine learning (ML) tahun 2014 sampai 2021 sehingga dapat memberikan jawaban atas pertanyaan penelitian kajian ini. Hasil analisis terhadap 24 artikel terpilih berdasarkan kriteria inklusi-eksklusi menunjukan bahwa kajian VTH dengan sentinel dan ML, umumnya mengkombinasikan sentinel dengan citra lain, seperti ALOS-2 L Band, Landsat, ALOS DSM dan data DEM.  Band S2-spektral, S2-indeks vegetasi, slope, S1-backscatter, elevation, adalah variable variable yang paling banyak digunakan sebagai prediktor. Algoritma RF (random forest regression), SVR (support vector regression), MLR (multi linear regression) dan kNN (k-nearest neighbor merupakan algotirma yang ditemukan paling banyak digunakan. Potensi peningkatan akurasi hasil pendugaan VTH secara signifikan dapat dilakukan dengan menambahkan faktor lingkungan sebagai variabel prediktor. Studi Mauya et al, Reis et al dan Chen et al yang mengkombinasikan data optis, SAR dan topografi, melaporkan adanya peningkatan akurasi pendugaan VTH yang signifikan. Selanjutnya Mauya et al mengemukakan bahwa pendekatan rata rata tertimbang dari piksel dalam mengekstraksi variabel dari citra berdasarkan posisi plot lapangan menghasilkan model pendugaan yang lebih baik dari pada menggunakan pendekatan centroid. Optimalisasi pemanfaatan band red edge dalam pendugaan VTH yang dilakukan oleh Jiang et al, Ahmadi et al dan Hu et al, menunjukan koleasi yang lebih signifikan antara band red edge dengan VTH daripada fitur lain yang diturunkan dari S2. Chyrysafis membuktikan bahwa modifikasi rumus indeks vegetasi dengan mengganti band (NIR) dengan band red edge terbukti meningkatkan korelasinya dengan volume tegakan secara signifikan dan berkinerja sangat baik pada algoritma RF, SVM maupun MLR dalam menduga VTH. Metode geostatistik kriging yang dilakukan Chen et al dan Bolat, menyimpulkan bahwa SVR-kriging dan Regresi-kriging masing-masing mengungguli kinerja SVR dan memiliki akurasi yang lebih baik dalam memprediksi VTH. Katakunci: Citra Sentinel, Machine Learning, Tegakan Hutan
Kualitas Madu Lebah Apis dorsata Desa Semalah Kabupaten Kapuas Hulu Kalimantan Barat Hilda Aqua Kusuma Wardhani; Desi Ratnasari; Siti Nur Kotimah
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.993 KB) | DOI: 10.33772/biowallacea.v9i2.28720

Abstract

Honey is a sweet natural liquid produced by honey bees from the nectar of plant flowers (floral nectar), other parts of plants (extra floral nectar), or insect excretions. One of the largest honey-producing areas in Indonesia is located in Semalah Village, Kapuas Hulu Regency, West Kalimantan. This study aims to analyze the quality of Apis dorsata honey bees produced in Semalah Village, Kapuas Hulu Regency. This research was conducted from January to March 2022. The sampling of tikung and lalau honey from Apis dorsata bees was taken by purposive sampling technique. The sample then were analyzed for physical quality (smell and taste) and chemical (water content, acidity, HMF levels, glucose, sucrose, and diastase enzyme activity) at the Food Technology Laboratory, Faculty of Engineering, Pasundan University, Bandung. The data was then analyzed descriptively and adjusted to the Indonesian National Standard (SNI) Number 8664:2018 regarding honey. Based on the results of the organleptic test, the two samples of tikung honey and lalau honey of Apis dorsata bees from Semalah Village, Kapuas Hulu Regency had honey characteristics by SNI number 8664 of 2018, and based on the results of the chemical test of honey, all test parameters (acidity, hydroxymethylfurfural levels, glucose, sucrose, and diastase enzyme activity) in accordance with SNI number 8664 of 2018 concerning honey, except for the water content parameter. The water content in samples of tikung honey and lalau honey of Apis dorsata bees from Semalah village, Kapuas Hulu Regency did not meet the standards set by SNI number 8664 of 2018. Keywords: Quality, Honey, Apis dorsata, Semalah Village  Abstrak  Madu adalah cairan alami dengan rasa manis yang dihasilkan oleh lebah madu dari nektar bunga tanaman (floral nektar), bagian lain dari tanaman (ekstra floral nektar) atau ekskresi serangga. Kalimantan Barat khususnya Kabupaten Kapuas Hulu merupakan salah satu daerah penghasil madu terbesar di Indonesia. Penelitian ini bertujuan untuk menganalisis kualitas madu lebah Apis dorsata yang dihasilkan di Desa Semalah Kabupaten Kapuas Hulu. Penelitian ini dilaksanakan pada bulan Januari sampai Maret 2022. Pengambilan sampel madu tikung dan lalau lebah Apis dorsata dilakukan dengan teknik purposive sampling. Sampel selanjutnya akan dianalisis kualitas fisik (bau dan rasa) dan kimia (kadar air, kadar HMF, keasaman, glukosa, sukrosa, dan aktivitas enzim diastase) di Laboratorium Teknologi Pangan Fakultas Teknik Universitas Pasundan Bandung. Data kemudian dianalisis secara deskriptif dan disesuaikan dengan Standar Nasional Indonesia (SNI) Nomor 8664:2018 tentang madu. Berdasarkan hasil uji organleptik, kedua sampel madu tikung dan madu lalau lebah Apis dorsata asal Desa Semalah Kabupaten Kapuas Hulu memiliki karakter madu sesuai dengan SNI nomor 8664 tahun 2018 dan berdasarkan hasil uji kimia madu, seluruh paramater uji (keasaman, kadar hidroksimetilfurfural, glukosa, sukrosa, dan aktivitas enzim diastase) sesuai dengan SNI nomor 8664 tahun 2018 tentang madu, kecuali pada parameter kadar air. Kadar air pada sampel madu tikung dan madu lalau lebah Apis dorsata asal desa Semalah Kabupaten Kapuas Hulu tidak memenuhi standar yang sudah ditetapkan oleh SNI nomor 8664 tahun 2018. Kata kunci: Kualitas, Madu, Apis dorsata, Desa Semalah
Analisis Kadar Nitrogen, Fosfor dan Kalium Pada Lahan Tambang Aspal Buton Peliyarni Peliyarni; Muh. Noor Azizu; Wulan Riski Yanti; Ridwan Ridwan
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.602 KB) | DOI: 10.33772/biowallacea.v9i2.27830

Abstract

Asphalt resources in Buton Island, Southeast Sulawesi Province are the only natural asphalt deposits in Indonesia. In addition to Indonesia, natural asphalt deposits are found in the Trinidad Islands, Albania and Iraq which are used for road coatings, building roofs, mastic flooring, paving mixes and paint mixtures. This study analyzes the levels of Nitrogen, Phosphorus and Kaliam at three asphalt mining locations, namely: heaps from asphalt processing, unprocessed asphalt land and locations from mining processing. The sample was taken from Nambo Village, Lasalimu District, Buton Regency, where the entire area is an asphalt mine. This study aims to provide solutions to the community regarding the handling of nutrient levels in asphalt mining land so that it can be used as agricultural land. The research was conducted through several stages including: Preparation of Tools and Materials; Sample Preparation; Analysis of Soil Nutrient Levels (includes: elemental N analysis, elemental analysis of P and analysis of elemental K). The results obtained that the highest soil nitrogen content was found in asphalt mining waste, namely 0.39% at a depth of 25 cm and 0.27% at a depth of 50 cm, while the lowest nitrogen content was found in soil without mining activities, namely 0.1 at a depth of 50 cm. at a depth of 25 cm and 0.07 at a depth of 50 cm. The highest phosphorus levels were found in soil without mining activities, namely 1302 mg/100g at a depth of 25 cm and 1518 mg/100g at a depth of 50 cm, while the lowest phosphorus levels were found in ex-mining soil, namely 329 mg/100g at a depth of 25 cm and 405 g. mg/100g at a depth of 50 cm. The highest potassium levels were found in soil without mining activities, namely 569 mg/100g at a depth of 25 cm and 521 mg/100g at a depth of 50 cm, while the lowest potassium levels were found in ex-mining soil, which was 127 mg/100g at a depth of 25 cm. and 128 mg/100g at a depth of 50 cm.
Analisis Sifat Fisik dan Magnetik Tanah di Bawah Tegakkan Pohon Kakao, Pohon Kelapa dan Jambu Mete Rosliana Eso; La Ode Saifuddin; Sitti Kasmiati; Mardiana N; Arman Arman; Husein Husein; Tahir Tahir
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (588.765 KB) | DOI: 10.33772/biowallacea.v9i2.28544

Abstract

This study aims to analyze the physical properties of soil, such as texture, color, temperature, moisture content, bulk density and permeability as well as the magnetic properties of the soil under a tree stand of cashew, coconut and cocoa. This research was conducted on mixed farms where there are cashew, coconut and cocoa trees close to each other. Samples were taken vertically from top soil to a depth of 100 cm. The results showed that the soil color and texture of the soil under cashew, coconut and cocoa trees tended to be the same considering the locations of these three trees were very close together, but the soil texture at a depth of 50-100 cm tended to vary for these three tree species. Likewise, the water content and the ability to pass water vary from one to another following the trend of changes in bulk density and soil texture. The variation in magnetic susceptibility values which increased towards the top of the soil profile both under cashew tree stands and cocoa tree stands followed the trend of normal soil variation where superparamagnetic grain enrichment occurred and was largely influenced by pedogenesis factors. Although the magnetic properties of both are the same as well as in the paramagnetic category, the magnetic susceptibility value under cashew tree stands is greater than that under cocoa tree stands.
Analisis Perkerabatan Drosophila ananassae Dari Daratan Kendari Suriana Suriana; Dwi Arinto Adi; Nasaruddin Nasaruddin
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 1 (2022): BioWallacea Universitas Halu Oleo
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.979 KB) | DOI: 10.33772/biowallacea.v9i1.12478

Abstract

D. ananassae kinship catched on Kendari mainland had been analized by crossing beetween D. Ananassae from Kendari, Pondidaha and Lasolo regions, experiment method conducted with 3 resiprocal each, Data conversioned in isolation indexes and tested with Duncan’s test. Isolation index of D.ananassae from Kendari region with F-count =4.10, greater than F-tab (3.11), average of isolation indexes was 0.13, that mean kinship of D.ananassae from Kendari mainland (Kendari, Pondidaha and Lasolo) were closed enough one another and assumed that they were same species.Keywords: D. ananassae, kinship, Kendari mainland                                                    AbstrakKekerabatan D. Ananassae yang ditangkap didaratan Kendari telah dianalisis dengan persilangan antara D. Ananassae yang ditangkap dari daerah Kendari, Pondidaha dan Lasolo. Dengan metode eksperimen yang terdiri dari 3 kali ulangan untuk setiap perlakuan, data yang diperoleh dikonversi menjadi data indeks isolasi dan diuji dengan analisis varians dan dilanjutkan dengan uji Duncan.  Hasil analisis menunjukkan bahwa indeks isolasi seksual D. Ananassae di daratan Kendari mempunyai F-hitung sebesar 4,10 dan lebih besar daripada F-tabel sebesar 3,11.  rata-rata indeks isolasi seksual yang didapatkan adalah sebesar 0,13, hal ini menunjukkan bahwa kekerabatan D. Ananassae di daratan Kendari cukup dekat dan diduga D. Ananassae yang ditangkap di Kendari, Pondidaha dan Lasolo berada pada level spesies yang sama.Key words: D. ananassae, perkerabatan, daratan Kendari
Keanekaragaman Hayati Serangga Polinator di Perkebunan Kakao Sitti Wirdhana Ahmad; Amirullah Amirullah; Desi Afdaliana; Sitti Hadijah Sabarwati
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 1 (2022): BioWallacea Universitas Halu Oleo
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.136 KB) | DOI: 10.33772/biowallacea.v9i1.24253

Abstract

The research of the diversity of pollinator insects species in Cocoa plantations in Puudongi village, Kolono region, South  Konawe district, Southeast Sulawesi was conducted in 2016. Ssample collecting was conducted by using yellow pan traps, light traps, adhesive trap and sweep net. Insects diversity was analyzed by using Shannon-Wienner formula. Data presented in tables and graphics. The results showed that the insect pollinator species  consist of 9 species in 7 families. Insects pollinators family are Formicidae, Colydiidae, Syrphidae, Colletidae, Vespidae, Tenthredinidae, and Curculionidae. while those identified to the species level is Anoplolepsis sp., Iridomyrmex sp. and Oechopila smaraghdina. Value insect pollinator diversity found among the sites that H '= 1.96 which indicates that the level of diversity of insect pollinators on plantations Cocoa (Theobroma cacao L.) in the Puudongi village, Kolono region, district East Konawe, South East Sulawesi in the medium category.Keywords: Biodiversity, Insect Pollinators, Cacao Plantation.AbstrakPenelitian tentang keanekaragaman serangga polinator di perkebunan kakao di Desa Puudongi Kecamatana Kolono, Kabupatan Konawe Selatan telah dilakukan pada tahun 2016. Sample dikumpulkan dengan menggunakan yellow pan trap, light trap, adhesive trap dan sweep net. Nilai keanekaragaman dihitung dengan menggunakan rumus Shanon-Wienner. Data disajikan dalam bentuk tabel dan gambar. Hasil penelitian menunjukkan bahwa ditemukan 9 spesies serangga polinator dari 7 famili yaitu famili Formicidae, Colydiidae, Syrphidae, Colletidae, Vespidae, Tenthredinidae, Curculionidae, sedangkan yang teridentifikasi sampai pada tingkat spesies yaitu Anoplolepsis sp., Iridomyrmex sp. dan Oechopila smaraghdina. Nilai keanekaragaman serangga polinator yang ditemukan pada lokasi penelitian yaitu H'= 1,96 yang menunjukkan bahwa tingkat keanekaragaman serangga pollinator pada perkebunan Kakao (Theobroma cacao L.) di Desa Puudongi Kecamatan Kolono, Kabupatan Konawe Selatan Sulawesi Tenggara berada dalam kategori sedang.Kata Kunci : Keanekaragaman, Serangga Polinator, Perkebunan Kakao.
Efektivitas Ekstrak Daun Kopi Robusta (Coffea canephora) Sebagai Repelan Terhadap Aedes aegypti Laurencia Alimin; Endah Setyaningrum; Dian Isti Angraini
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 1 (2022): BioWallacea Universitas Halu Oleo
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.153 KB) | DOI: 10.33772/biowallacea.v9i1.25550

Abstract

Dengue Hemorrhagic Fever (DHF) is an endemic disease in various tropical countries that is transmitted through the bite of  Aedes aegypti. One of the prevention of DHF transmission is by using repellent. Repellents that are usually used in the community have high side effects. So we need to find a replacement made from natural components. Flavonoid is one of the compounds in robusta coffee leaves that has a repellent effect. This study used analytical research with a pure experimental research design namely the Posttest Only Control Group Design in the form of a Complete Randomized Design (CRD) conducted from November 2019 to January 2020, consisting of five treatments (negative control, concentration of 10%, 25%, 50% and positive control) and repeated five times. Data were analyzed with One Way Anova, Post Hoc Bonferroni and Probit analysis. The results of study of robusta coffee leaf extract as repellent at a concentration of 10%, 25% and 50% gave protection power to 41.21%, 68.69% and 81.46% with ED50 concentration was 16.834% and ED99 contained at a concentration of 49.788%. The conclusion is the higher the concentration of robusta coffee leaves extract, the higher its protective power as repellent against Aedes aegypti.Keywords:Aedes aegypti, Robusta Coffee Leaves, Repellent AbstrakDemam Berdarah Dengue (DBD) merupakan penyakit endemis diberbagai negara beriklim tropis yang ditularkan melalui gigitan nyamuk Aedes aegypti. Salah satu pencegahan penularan penyakit DBD adalah dengan menggunakan repelan. Repelan yang digunakan di masyarakat umumnya memiliki efek samping yang tinggi sehingga upaya pencarian repelan berbahan alami lebih diutamakan. Flavonoid merupakan salah satu senyawa yang terdapat di dalam daun kopi robusta memiliki efek sebagai repelan. Penelitian ini menggunakan jenis penelitian analitik dengan desain penelitian eksperimental murni yaitu Posttest Only Control Group Design yang berupa Rancangan Acak Lengkap (RAL) dilakukan dari bulan November hingga Januari 2020, terdiri dari lima perlakuan (kontrol negatif, konsentrasi 10%, 25%, 50% dan kontrol positif) dan diulang sebanyak lima kali. Data yang didapatkan dianalisis dengan menggunakan One Way Anova, Post Hoc Bonferroni dan analisis Probit. Hasil penelitian didapatkan bahwa ekstrak daun kopi robusta sebagai repelan pada konsentrasi 10%, 25% dan 50% memberikan daya proteksi sebesar 41,21%, 68,69% dan 81,46% dengan ED50 adalah konsentrasi 16,834% dan ED99 terdapat pada konsentrasi 49,788%. Kesimpulannya semakin tinggi konsentrasi ekstrak daun kopi robusta semakin tinggi daya proteksi sebagai repelan terhadap Aedes aegypti. Kata kunci: Aedes aegypti, Daun Kopi Robusta, Repelan.
Keanekaragaman Microarthropoda infauna Tanah Pada Areal Lahan Karst Bekas Kebun yang Diberakan di Kabupaten Buton Tengah Muhsin Muhsin; Amirullah Amirullah; Muhammad Bayu Santoso; Indrawati Indrawati
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 1 (2022): BioWallacea Universitas Halu Oleo
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.296 KB) | DOI: 10.33772/biowallacea.v9i1.24736

Abstract

This study aims to determine the population and diversity of infauna microarthropoda (Acari and Collembola) found in the karst area of abandoned gardens in Central Buton Regency. This research is an explorative research conducted on karst land in the garden that is temporarily cultivated (Station 1) and land that has been abandoned for 10 years (Station 2), each station consists of 4 sampling plots. Soil sampling is done by using Soil Ring Sampler with a diameter of 7.5 cm and a length of 10 cm. Soil samples were extracted by using Tullgreen Funnel and of the data analyzed  with Shannon-Wienner diversity index formula with descriptive data presentation. Microarthropoda infauna (Acari) at station 1 obtained as many as 6 species from 6 genera with a total of 45 individuals. At station 2 as many as 3 species of 3 genera, with a total of 20 individuals. Types of microarthropoda infauna (Acari) found in 2 research stations namely; Carpoglyphus lactis, Lasioacarus morsei, Pygmephorus spinosus, Suidasia pontifica, Tetranychus urticae  and Tyrophagus putrescentiae. The diversity of infauna microarthropodes at station 1 was 1,844 and station 2 was 1,394 in the moderate category.Keywords: Diversity, Microarthropod Infauna, Karst Area, Abandoned Garden AbstrakPenelitian ini bertujuan untuk mengetahui populasi dan keanekaragaman mikroarthropoda infauna (Acari) yang terdapat pada areal lahan karst bekas kebun yang diberakan di Kabupaten Buton Tengah. Penelitian ini merupakan penelitian eksploratif yang dilakukan pada lahan karst di kebun yang sementara diolah (Stasiun 1) dan lahan yang ditinggalkan selama 10 tahun (Stasiun 2),  masing-masing stasiun terdiri dari 4 plot pengambilan sampel. Pengambilan sampel tanah dilakukan  menggunakan Soil Ring Sampler dengan ukuran diameter 7,5 cm dan panjang 10 cm. Sampel tanah di ekstraksi menggunakan Tullgreen Funnel dan dianalisis dengan rumus indeks keanekaragaman Shannon-Wienner dengan penyajian data yang bersifat deskriptif. Mikroarthropoda infauna (Acari) pada stasiun 1 diperoleh sebanyak 6 spesies dari 6 genus dengan total 45 individu. Pada stasiun 2 sebanyak 3 spesies dari 3 genus, dengan total sebanyak 20 individu. Jenis Mikroarthropoda infauna (Acari) yang ditemukan pada ke 2 stasiun penelitian yaitu; Carpoglyphus lactis, Lasioacarus morsei, Pygmephorus spinosus, Suidasia pontifica, Tetranychus urticae dan Tyrophagus putrescentiae. Keanekaragaman Mikroarthropoda infauna (Acari) pada stasiun 1 sebesar 1,844  dan stasiun 2 sebesar 1,394 yang termasuk dalam kategori sedang.Katakunci: Keanekaragaman, Mikroarthropoda Infauna, Kawasan Karst, Kebun diberakan
Efektivitas Ekstrak Etanol Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) Sebagai Antipiretik Pada Mencit (Mus muscullus L.) Hiperpireksia Muzuni Muzuni; Uhra Ali; Wa Ode Harlis
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 1 (2022): BioWallacea Universitas Halu Oleo
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v9i1.25505

Abstract

This study aims to find out the effectiveness of ethanol extract rhizome curcuma (Curcuma xanthorrhiza Roxb.) as an antipyretic in mice(Mus muscullus L.) hyperpireksia. This study is an experimental study with the Complete Randomized Design (RAL) method. A total of 15 male mice weighing 30-35 grams were divided into 5 treatment groups namely KN (drinking water), KP (paracetamol), P1 (extract 3 mg/gr BB), P2 (extract 8 mg/gr BB) and P3 (extract 14 mg/gr BB). Hyperpyreksia is obtained by inducing mice with 10% peptons. When it reaches above normal temperatures (35.50C - 37.00C), it is given extracts according to the treatment group and measured its effect at minutes 30, 60 and 90. The data was analyzed using ANOVA with α=0.05 and advanced tests of LSD. The results showed P3 was more effective and faster at lowering body temperature, compared to KN, P1 and P2. Seen in the 90th minute the body temperature squeaked P3 (35.960C) back to normal temperatures and closer to KP (35,760C), after which followed by P2 (36.30C). KN (38,630C) and P1 (37,630C) are still categorized as high, as they have not returned to normal temperatures. The results can be concluded that the ethanol extract of curcuma rhizome has antipyretic activity. ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol rimpang temulawak(Curcuma xanthorrhiza Roxb.) sebagai antipiretik pada mencitMus muscullus L.) hiperpireksia. Penelitian ini merupakan penelitian eksperimental dengan metode Rancangan Acak Lengkap (RAL). Sebanyak 15 ekor mencit jantan berat 30-35 gram dibagi dalam 5 kelompok perlakuan yaitu KN (air minum), KP (parasetamol), P1 (ekstrak 3 mg/gr BB), P2 (ekstrak 8 mg/gr BB) dan P3 (ekstrak 14 mg/gr BB). Mencit hiperpireksia diperoleh dengan cara menginduksi mencit dengan pepton 10 %. Ketika mencapai di atas suhu normal (35,50C - 37,00C), maka diberikan ekstrak sesuai kelompok perlakuan dan diukur pengaruhnya pada menit ke 30, 60 dan 90. Data dianalisis menggunakan ANOVA dengan α=0,05 dan uji lanjut LSD. Hasil penelitian menunjukkan P3 lebih efektif dan lebih cepat menurunkan suhu tubuh mencit, dibandingkan dengan KN, P1 dan P2. Dilihat pada menit ke-90 suhu tubuh mencit P3 (35,960C) kembali ke suhu normal dan lebih mendekati KP (35,760C), setelah itu diikuti P2 (36,30C). KN (38,630C) dan P1 (37,630C) masih dikategorikan tinggi, karena belum kembali ke suhu normal. Hasil tersebut dapat disimpulkan bahwa ekstrak etanol rimpang temulawak memiliki aktivitas antipiretik.Kata Kunci : Antipiretik, Temulawak, Mencit, Efektitivitas, Hiperpireksia.

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