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BioWallacea Journal of Biological Research

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Published by Universitas Halu Oleo

ISSN : 23556404 EISSN : 26856360 DOI : https://doi.org/10.1234/1234

Core Subject : Health, Agriculture,

Agriculture, Biological Sciences & Forestry Veterinary

BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) telah memiliki ISSN 2355-6404 (print) dan ISSN 2685-6360 (online) yang merupakan salah satu jurnal nasional dan diterbitkan oleh Jurusan Biologi FMIPA Universitas Halu Oleo. Jurnal ini fokus pada ilmu biologi dan serumpun. Jurnal BioWallacea menerima naskah-naskah terbaik dari penulis yang bersifat asli hasil penelitian maupun telaah (review). Naskah ditulis dengan baik untuk setiap topik berkaitan dengan biologi yang berkembang saat ini serta bidang-bidang lain, termasuk: Penelitian Ekologi Fisiologi Ekofisiologi Taksonomi Botani Zoologi Mikrobiologi Biologi Laut Ilmu terapan berkaitan dengan ilmu Biologi seperti Bioteknologi, Biokimia, dan Biologi Sel serta Molekuler. Penelitian yang berkaitan isu lingkungan, termasuk Analisis Mengenai Dampak Lingkungan (AMDAL).

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 157 Documents

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Pengaruh Kombinasi Media Tanah dan Pasir Terhadap Penurunan Kadar BOD dan COD pada Limbah Domestik Rumah Makan dengan Sistem Wetland Menggunakan Tumbuhan Cyprus Lily Oktavia; Catur Lega Wibisono; Muchammad Tamyiz
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 1 (2021): BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research)
Publisher : University of Halu Oleo

The development of restaurant businesses in Sidoarjo City is quite rapid, and this will of course be accompanied by an increase in domestic waste originating from a restaurant called greywater. Restaurant wastewater contains a lot of organic content and often without prior treatment is discharged directly into receiving water bodies. The purpose of this study was to determine the reduction in BOD and COD levels for the processing of restaurant wastewater using a contructed wetland with Cyperus Rotundus as the remediator. The research stage will be carried out using a laboratory scale using acclimatization as the adaptation stage of the Cyperus Rotundus plant and the crontructed wetland reactor as phytoremediation processing. Based on the results and discussion, it can be concluded that there was a decrease in BOD and COD levels in restaurant liquid waste using the Constructed Wetland System and Cyperus Rotundus plants. The percentage reduction in the largest BOD content in clay media with the number of Cyperus Rotundus plants of 3 stems gave the highest BOD removal efficiency, namely 70% at 4 days of detention time. While the largest percentage reduction in COD levels in clay media with the number of Cyperus Rotundus plants as many as 3 stems gave the highest COD removal efficiency, namely 67% at 4 days detention time.

Makroinvertebrata Sebagai Bioindikator Kualitas Air Embung Roka Kecamatan Belo, Kabupaten Bima, Nusa Tenggara Barat M. NORIS; Jamili Jamili; LOAP Rudia
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 1 (2021): BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research)
Publisher : University of Halu Oleo

Jenis penelitian ini adalah penelitian deskriptif. Metode penelitian adalah metode survey eksploratif (makroinvertebrata). Objek penelitian adalah keberagaman jenis makroinvertebrata. Lokasi Penelitian Adalah Embung Roka, Kecamatan Belo, Kabupaten Bima, Nusa Tenggara Barat. Fokus penelitian ini adalah mengenai kelimpahanan makroinvertebratasebagai bioindikator kualitas air embung roka. Tekhnik pengumpulan data dengan melakukan pengambilan sampel makroinvertebrata pada 3 stasiun (hulu, tengah, dan hilir), kemudianmengidentifikasimakroinvertebrata yang dijumpai pada area lokasi penelitian. Analisis data dilakukan dengan cara menghitung indeks keanekaragaman jenis Shannon and Wiener (H’), Indeks Dominansi (C) dan Indeks Kemerataan (E) dari Makoinvertebrata sebagai bioindikator kualitas air embung roka. Hasil penelitian menunjukkan bahwa Makroinvertebrata merupakan bioindikator yang dapat dimanfaatkan sebagai parameter menentukan kualitas air perairan Indeks nilai keanekaragaman (H’) berkisar 1.688 tergolong dalam kategori sedang, indeks kemerataan (E) berkisar 0.759 tergolong tinggi dan indeks dominansi (C) berkisar 2.784 tergolong tinggi. Familia Cichilidae, Ampullariidae, Cichonididae, Dan Gerridae ditemukan relatif mendominasi ekosistem sehingga dapat menjadi parameter bahwa kualitas perairan tergolong baik.

Struktur Vegetasi dan Simpanan Karbon Sedimen Kawasan Regenerasi Mangrove Avicennia lanata (Ridley). di Pesisir Kabupaten Muna La Ode Abdul Fajar Hasidu; Arif Prasetya; Al Tafakur La Ode
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.953 KB)

This study aimed to determine the structure and composition of natural regenerated mangrove, also to analyze these soil C and N content. This study was conducted in natural regenerated mangrove at in-active pond in Muna Regency, Southeast Sulawesi on June-August 2021. The data collection of mangrove vegetation by quadratic transect method, prependicuar to the river, with 6 subplots sized 100m2. Species identification according to Panduan Pengenalan Mangrove Indonesia, by Noor YR et al. 2006. Meanwhile, the methods for sediment collection was done by purposive sampling to determine corring site. The sediment was collected by Peat Auger Set for 50 cm of depth. The sediment sample then separated based on depth i.e. 0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm, and 40-50 cm. The samples were dried with temperature 65oC during 10 days. The C and N content were analyzed by Spectrofotometer. Moreover, water content as well as bulk density was determined. The statistical analysis of One Way-Anova was done to determine the differences of bulk density, water, C, and N content on each soil layer.The result showed that the structure and composition of mangrove which found in this location was weaning mangrove (DBH < 10cm). The class of DBH size was dominated by 3,00cm - 3,99 cm (81 ind), 2,00cm – 2,99cm (62 ind), 4,00cm – 4,99cm (52 ind), 5,00cm – 5,99cm (42 ind), 7,00cm – 7,99cm (32 ind), and 6,00cm – 6,99cm (26 ind) respectively. The class of DBH size of 8,00 and 9,00 were found in small amount. The total density of vegetation was categoryzed into high density category, based on Kepmen LH No 201, year 2004 (5183,33 ind/ha). The vegetation formed into the early growing stage. The result of water content, and bulk density showed various results. The highest water content was on the surface layer of soil (0-10 cm) was 35,39±8,61%.Meanwhile, the highest bulk density was on the 20-30 cm of soil layer, was 1,49±0,29 g/cm3. The result of C and N content showed various results on each soil layer. The highest of C content was on the surface layer (0-10 cm), was 2,13±0,76%, and the lowest was on 30-40 cm of soil layer, was 1,72±0,13%. In the other hand, the highest N content was on 40-50 of soil layer, was 0,82±0,29%, and the lowest was on 30-40 cm of soil layer, was 0,57±0,14%. Although there were difference of water content, bulk density, C, and N content on each soil layer, but these differences was not significant statistically (α > 0,05). Commonly, the A. lanata species was dominated in this in-active pond area. It was caused by the environmental condition was enclosed because of soil bund wall of pond, so it is confined to another species to regenerate naturally in this area. Furthermore, the structure of A. lanata seeds which have small size facilitates its spread by the current, thus providing a opportunity for seeds spreaded, growth and regenerated naturallyKey word: A. lanata, natural regeneration, C sedimen, N content

Nepenthes di Gunung Sibuatan, Provinsi Sumatera Utara, Indonesia Mhd. Rafi'i Ma'arif Tarigan; Yusran Efendi Ritonga
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

ABSTRACT This research aims at determining the types of Nepenthes in the Mount Sibuatan area, Karo District, North Sumatera Province, Indonesia. The research was conducted in March 2021, using explorative research methods. Samples collected in fresh form. Identification was carried out using the procedures of book references Flora of Java Volume I, Nepenthes of Sumatra, Field Guide to the Pitcher Plants of Sumatra and Java. The results of the research found five species of Nepenthes namely N. pectinata Danser, N. spectabilis Danser, N. tobaica Danser and two species are natural crosses, namely N. pectinata x spectabilis, and N. pectinata x tobaica at an altitude of 1,796-2,457 m asl with a temperature 15.3°C-21.2°C. Key words: Nepenthes, Characterization of Nepenthes, Mount Sibuatan

Uji Toksisitas Ekstrak Metanol Etlingera calophrys Dengan Metode Brine Shrimp Lethality Test (BSLT) Megawati Megawati; Muhamad Jalil Baari; Carla Wulandari Sabandar
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (588.681 KB)

Pada penelitian ini, dilakukan skrining fitokimia utuk menganalisis kandungan metabolit sekunder/senyawa aktif dalam ekstrak metanol bagian-bagian tumbuhan Etlingera calophrys yang meliputi daun, buah, batang, dan rimpang, yang dilanjutkan dengan pengujian aktivitas sitotoksisitasnya. Skrining fitokimia dilakukan untuk memastikan adanya kandungan senyawa aktif, sedangkan uji sitotoksisitas dilakukan dengan metode Brine Shrimp Lethality Test menggunakan larva Artemia salina. hasil skrining menunjukkan adanya senyawa alkaloid, flavonoid, fenolik, terpenoid/steroid, dan saponin yang tersebar di seluruh bagian Etlingera calophrys dengan bagian batang mengandung paling banyak senyawa metabolit sekunder. Sementara itu, berdasarkan hasil analisis sitotoksisitas, diketahui nilai LC50 pada jaringan daun, buah, batang, dan rimpang masing masing yaitu 65.2 ± 8.9 ppm, 65.7 ± 6.4 ppm, 132.3 ± 12.8 ppm, dan 84.1 ± 10.3 ppm. Hasil tersebut menunjukkan bahwa Etlingera calophrys berpotensi sebagai antioksidan/antikanker alami sekaligus menjadi pilihan alternatif obat di masa yang akan datang.

Perbandingan dan Pengaruh Penyimpanan Sampel Serum Terhadap Kualitas DNA: Pengamatan Selama 1 Tahun Tiara Mayang P. Lio; Sanatang Sanatang; Aldiana Aldiana
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 1 (2021): BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research)
Publisher : University of Halu Oleo

Molecular diagnosis is a method of diagnosis that aims to understand the molecular mechanism of a disease in each individual patient (personalized medicine / dentistry) as well as the identification process in the forensic field. This method will be very beneficial in this modern world of health. One of the molecular identification techniques that can be used as a means is the DNA amplification technique using the PCR method. This technique is able to multiply the DNA strand of the sample so that it can be analyzed more clearly. Where in the process good DNA quality can be obtained from good samples too. This is a basic requirement that must be met in molecular studies, especially in DNA fingerprinting, whereas in field practice unexpected things often occur either in the sample collection process or the availability of chemicals which result in a delay in a DNA extraction process. While the long storage time can cause damage to blood cells including DNA due to natural degradation or by microorganisms (fungi and bacteria). Comparative study and the effect of storage of serum samples on DNA quality: Observation for 1 year was a descriptive analytic study to see whether there was an effect of storage at -40C on DNA quality. This study used an analytic observational method with a cross-sectional approach. The research sample was blood serum. Genetic analysis is performed by isolating DNA from serum using routine procedures. Based on the research results, it was found that the Sig. DNA levels in 2019 and 2020 were 0.018 and the Sig. DNA purity in 2019 and 2020 was 0.00. This shows the effect of 1 year storage time on the quality of DNA from blood serum samples.

Identifikasi Isolat Streptomyces hygroscopicus INA CC A497 sebagai Antimalaria : Uji Pendahulu Rosa Salsabila Reza; Endah Setyaningrum; Nismah Nukmal; Achmad Arifiyanto
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Streptomyces sp is known as a bacterium that is able to produce secondary metabolites that act as antiparasitic, antitumor, anti-oxidant, plant growth, herbicide, pesticide, and many others so that it is often used as an antibiotic in the form of drugs. This study aims to identify the bacteria InaCC A497 Streptomyces hygrocipcus subsp Jinggangensis collection from the Indonesian Culture Collection (InaCC) Lipi Cibinong which will be used as an antimalarial. The tests carried out were biochemical tests (sugar test, indole test, and motile test), and observation of growth curves for 6 consecutive days using a UV-VIS Spectrophotometer. The results showed that the bacteria InaCC A497 Streptomyces hygrocipcus subsp Jinggangensis has the potential as an anti-malarial.Keywords: Streptomyces hygroscopicus, Anti-malaria, UV-VIS Spectrophotometer, Secondary metabolites, Bacteria ABSTRAKStreptomyces sp. dikenal sebagai bakteri yang mampu menghasilkan metabolit sekunder yang berperan sebagai antiparasit, antitumor, antioksidan, pertumbuhan tanaman, herbisida, pestisida, dan lain-lain sehingga sering digunakan sebagai antibiotik dalam bentuk obat. Penelitian ini bertujuan untuk mengidentifikasi bakteri InaCC A497 Streptomyces hygrocipcus sub sp. Jinggangensis koleksi. Koleksi Kultur Indonesia (InaCC) LIPI Cibinong yang akan digunakan sebagai antimalaria. Pengujian yang dilakukan adalah uji biokimia (uji gula, uji indol, dan uji motil), dan pengamatan kurva pertumbuhan selama 6 hari berturut-turut menggunakan Spektrofotometer UV-VIS. Hasil penelitian menunjukkan bahwa bakteri InaCC A497 Streptomyces hygrocipcus sub sp. Jinggangensis berpotensi sebagai antimalaria.Kata kunci: Streptomyces hygroscopicus, Antimalaria, Spektrofotometer UV-VIS, Metabolit sekunder, Bakteri

Jumlah Sel - Sel Spermatogenik Testis Mencit (Mus musculus L.) Setelah Pemberian Ekstrak Mangrove Bruguiera gymnorrhiza Lamk. Wa Ode Harlis; Jamili Jamili; Ernawati Ernawati
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Tujuan penelitian ini untuk mengatahui pengaruh ekstrak mangrove Bruguiera gymnorrhiza Lamk. terhadap komposisi sel spermatogenik testis mencit (Mus musculus L.). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 4 perlakuan dengan masing-masing enam ulangan, yaitu kontrol (Na CMC 0,5%), dan pemberian ekstrak daun, kulit batang dan buah mangrove Bruguiera gymnorrhiza Lamk. dosis 20mg/kg BB. Ekstrak diberikan secara oral selama 34 hari. Sehari setelah perlakuan berakhir, organ testis diisolasi dan dibuat sediaan preparat histologis. Parameter yang diamati yaitu jumlah sel-sel spermatogonia, sel spermatosit primer, sel spermatid dan kepadatan spermatozoa mencit. Metode perhitungan dilakukan secara langsung dengan mengamati sel-sel yang tersusun di tubulus seminiferus pada sediaan histologi testis untuk setiap perlakuan dan ulangan dengan menggunakan kamera mikroskopik dan software Image Raster dari Optilab. Data yang diperoleh dianalis dengan One Way Anova dan uji lanjut BNT (α=0,05) untuk perlakuan yang menunjukkan perbedaan nyata. Pemberian ekstrak daun, kulit batang dan buah mangrove Bruguiera gymnorrhiza (L) Lamk secara signifikan menurunkan jumlah sel sel spermatogenik mencit (Mus musculus L.) dengan susunan sel sel spermatogenik yang semakin tidak teratur, renggang dan lumen yang tidak terisi penuh spermatozoa. Penurunan jumlah sel-sel spermatogenik dalam penelitian ini terbanyak pada perlakuan ekstrak daun.Kata Kunci: Bruguiera gymnorrhiza Lamk, Spermatogonia, spermatosit primer, spermatid. Kepadatan spermatozoa, mencit jantan ABSTRACTThe purpose of this study was to determine the effect of the mangrove extract of Bruguiera gymnorrhiza Lamk. on the spermatogenic cell composition testicular of mice (Mus musculus L.). This study used a completely randomized design (CRD), consisting of 4 treatments with six replications each, namely control (Na CMC 0.5%), and administration of Bruguiera gymnorrhiza Lamk leaf, bark and mangrove fruit extracts. dose of 20mg / kg BW. The extract was administered orally for 34 days. The day after the treatment ended, the testicular organs were isolated and histological preparations were made. The parameters observed were the number of spermatogonia cells, primary spermatocytes, spermatid cells and the density of spermatozoa in mice. The calculation method was carried out directly by observing the cells arranged in the seminiferous tubules on the testicular histology for each treatment and replication using a microscopic camera and Image Raster software from Optilab. The data obtained were analyzed with One Way Anova and LSD further test (α = 0.05) for treatments that showed significant differences. The administration of leaf extract, stem bark and mangrove fruit Bruguiera gymnorrhiza (L) Lamk significantly reduced the number of spermatogenic cells of mice (Mus musculus L.) with increasingly irregular, loosened and lumen cells that were not fully filled with spermatozoa. The decrease in the number of spermatogenic cells in this study was mostly in the leaf extract treatment.Key words: Bruguiera gymnorrhiza (L) Lamk, spermatogonia, primary spermatocytes, spermatids, male mice

Efektivitas Gel Tepung Cacing Tanah (Lumbricus rubellus Hoff.) Sebagai Penyembuh Luka Sayat Pada Mencit (Mus muscullus L.) Wa Ode Harlis; Nasaruddin Nasaruddin; Nurhasana Nurhasana; Resman Resman
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Cuts are wounds that occur due to being cut by a sharp instrument. Earthworms (Lumbricus rubellus Hoff.) contain antibacterial compounds, arachidonic acid and proteins that help the growth of skin tissue. The formulation in the form of a gel is easier to use and has soothing, moisturizing properties, and easily penetrates the skin so that it provides a healing effect. This study aims to determine the effect of earthworm flour gel on wound healing in mice (Mus muscullus L.). This type of research is an experimental study with a completely randomized design (CRD). A total of 20 male mice were divided into 5 groups, namely positive control (Kalmicetine 10%), negative control (without treatment), K1 (earthworm flour gel concentration 3%), K2 (earthworm flour gel concentration 4%) and K3 (earthworm flour gel with a concentration of 4%). earthworm flour 5% concentration). The results showed that the average length of time for wound healing for each group was 11.75 days or 282 hours for a 3% concentration, 11.25 days or 270 hours for a 4% concentration, and 11 days or 264 hours for a 5% concentration, compared to positive control (Kalmicetine). 10%) 12 days or 288 hours and negative control for 14 days or 336 hours. The results of the study concluded that earthworm (Lumbricus rubellus Hoff.) flour gel was effective in accelerating the healing of cuts.Keywords: Cuts, Lumbricus rubellus, gelAbstrakLuka sayat adalah luka yang terjadi karena teriris oleh instrument yang tajam. Cacing tanah (Lumbricus rubellus Hoff.) mengandung senyawa antibakteri, asam arakidonat dan protein yang membantu pertumbuhan jaringan kulit. Formulasi dalam bentuk gel lebih mudah digunakan dan mempunyai sifat yang menyejukan, melembabkan, dan mudah berpenetrasi pada kulit sehingga memberikan efek penyembuhan. Penelitian ini bertujuan untuk mengetahui pengaruh gel tepung cacing tanah terhadap penyembuhan luka sayat pada mencit (Mus muscullus L.). Jenis penelitian adalah penelitian eksperimental dengan metode rancangan acak lengkap (RAL). Sebanyak 20 ekor mencit jantan dibagi dalam 5 kelompok yaitu kontrol positif (Kalmicetine 10%), kontrol negatif (tanpa perlakuan), K1 (gel tepung cacing tanah konsentrasi 3%), K2 (gel tepung cacing tanah konsentrasi 4%) dan K3 (gel tepung cacing tanah konsentrasi 5%). Hasil penelitian menunjukkan bahwa rerata lama waktu penyembuhan luka setiap kelompok adalah 11,75 hari atau 282 jam untuk konsentrasi 3%, 11.25 hari atau 270 jam untuk konsentrasi 4%, dan 11 hari atau 264 jam untuk konsentrasi 5%, dibandingkan kontrol positif (Kalmicetine 10%) 12 hari atau 288 jam dan kontrol negatif selama 14 hari atau 336 jam. Hasil penelitian disimpulkan bahwa gel tepung cacing tanah (Lumbricus rubellus Hoff.) efektif dalam mempercepat penyembuhan luka sayat.Kata Kunci: Luka sayat, Lumbricus rubellus, gel

Analisis Kemampuan Serapan CO2 dan Simpanan Karbon Pada Hutan Mangrove Di Desa Parida Kecamatan Lasalepa Kabupaten Muna La Ode Siwi; Basrudin Basrudin; Aminuddin Mane Kandari; Kahirun Kahirun; Herlan Hidayat; Mohammad Irsan
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

This study aims to determine the potential for CO2 uptake and carbon storage on the mangrove forest of Parida Village Lasalepa District Muna Regency. This research was conducted in January 2022 at Parida Village, Lasalepa District, Muna Regency. The sampling in this study used the purposive sampling method, the determination of the sampling was selected systematically with an intensity of 1%. The placement of the measuring plots is based on the zoning of the mangrove forest which is made of 3 (three) plots, where each plot is 15 m x 15 m (225 m2). The variables in this study were the diameter of an adult's chest height to determine above-ground biomass and carbon (above ground) without cutting down trees (non-destructive). Biomass is determined by Allometric equations. The carbon is determined by of the biomass. The results showed that the potential for CO2 absorption and carbon storage in mangroves in Parida Village, Lasalepa District, Muna Regency had an average CO2 absorption of 1057,49 ton.ha-1, and carbon storage 288,41 ton.ha-1. The carbon value is influenced by environmental conditions, the type of wood mass, the diameter of the mangrove trunk and the number of species contained in a plot.Keywords: Mangrove Forest, CO2Absorption,Carbon Storage. AbstrakPenelitian ini bertujuan untuk mengetahui potensi serapan CO2 dan simpanan karbon pada hutan mangrove di Desa Parida Kecamatan Lasalepa Kabupaten Muna.Penelitian ini dilaksanakan pada Bulan Januari 2022 bertempat di Desa Parida Kecamatan Lasalepa Kabupaten Muna.Adapun pengambilan sampel dalam penelitian ini menggunakan metode purposive sampling, penentuan pengambilan sampel dipilihsecara sistematis dengan intensitas 1%. Penempatan plot ukur didasarkan pada zonasi hutan mangrove yang dibuat 3 (tiga) plot, dimana masing-masing plot berukuran 15 m x 15 m (225 m2). Variabel dalam penelitian ini yaitu diameter batang untuk menentukan bioamassa dan jumlah karbon diatas permukaan tanah (above ground) dengan tanpa menebang pohon (non destuktif).Biomassa ditentukan dengan persamaan Allometrik.Adapun karbon ditentukan dengan ½ dari biomassa. Hasil penelitian menunjukan bahwa potensi serapan CO2 dan simpanan karbon pada hutan mangrove di Desa Parida Kecamatan Lasalepa Kabupaten Muna memiliki rata-rata serapan CO2 sebesar 1057,49ton.ha-1, serta simpanankarbon 288,41 ton.ha-1. Besar nilai karbon dipengaruhi oleh kondisi lingkungan, jenis masa kayu, diameter batang mangrove dan jumlah jenis yang terdapat dalam suatu plot.Kata Kunci : Hutan Mangrove, Serapan CO2, Simpanan Karbon

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