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BioWallacea Journal of Biological Research
Published by Universitas Halu Oleo
ISSN : 23556404     EISSN : 26856360     DOI : https://doi.org/10.1234/1234
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) telah memiliki ISSN 2355-6404 (print) dan ISSN 2685-6360 (online) yang merupakan salah satu jurnal nasional dan diterbitkan oleh Jurusan Biologi FMIPA Universitas Halu Oleo. Jurnal ini fokus pada ilmu biologi dan serumpun. Jurnal BioWallacea menerima naskah-naskah terbaik dari penulis yang bersifat asli hasil penelitian maupun telaah (review). Naskah ditulis dengan baik untuk setiap topik berkaitan dengan biologi yang berkembang saat ini serta bidang-bidang lain, termasuk: Penelitian Ekologi Fisiologi Ekofisiologi Taksonomi Botani Zoologi Mikrobiologi Biologi Laut Ilmu terapan berkaitan dengan ilmu Biologi seperti Bioteknologi, Biokimia, dan Biologi Sel serta Molekuler. Penelitian yang berkaitan isu lingkungan, termasuk Analisis Mengenai Dampak Lingkungan (AMDAL).
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 157 Documents
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KEANEKARAGAMAN JENIS BURUNG PADA AREAL PERKEBUNAN KELAPA SAWIT DI KECAMATAN BESULUTU KABUPATEN KONAWE SULAWESI TENGGARA Sitti Wirdhana Ahmad; Jamili Jamili; Mustang Mustang
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 3, No 1 (2016): BIodiversitas
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.495 KB) | DOI: 10.33772/biowallacea.v3i1.1482

Abstract

ABSTRACTThis research aims to know the diversity and apportionment’s of bird on oils palm plantation acreage at Besulutu area Konawe Regency South-east Sulawesi. This research was done at oils palm plantation area PT. Mega Utama Tani on September until October 2015. This research constitutes explorative research. In this research the researcher made three stations with the reason that each station has distinctive. The data of Bird was taking by use IPA (Indices Punctual Abundance method). IPA (Indices Punctual Abundance) method is bird watch method to be accounted deep given time range that at compounds by transect's method of lines Transect's line. Base on observational result, it was found as much 17 bird geneses with totaled bird individual 722 one most deep groups 7 ordo and 14 families. Shannon Wiener's diversity index I-III alternately each of 1,99, 1,96, 2,30. Bird diversity on station I and II Includes in low category, meanwhile III. Station comprises category be, apportionment Pielou's index I-III alternately each of 0,72, 0,79, 0,83. Base acquired apportionment point on three stations, the apportionment of these three stations is categorize as high.Keyword : Bird, Oil Palm Plantation, Diversity, Apportionment
DISTRIBUSI VERTIKAL GASTROPODA PADA MANGROVE Rhizophora apiculata DI TELUK KENDARI Muhsin Muhsin; Jamili Jamili; Hendra Hendra
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 3, No 1 (2016): BIodiversitas
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v3i1.1486

Abstract

ABSTRACTThe aim of this study was to know the vertical distribution of gastropoda at the mangrove Rhizophora apiculata in the Kendari Beach. Method whice isusingin research is transect line. The six transects lines and 20 quadrates 20x20 m for tree, 10x10 m for pole, 5x5 m for boundary pole, ang 2x2 m for seedling. Two samplings of mangrove tree, pole, boundary pole and seedling were chosen each quadrat for observation. The numbers of species and individuals of gastropods were counted in each organ of mangrove samplings (roots, stems, and leaves organs). The parameters of vertical distrubution of gastropods were analyzed including frequency and abundance. The results of this study showed that there exists 8 species of gastropods at the mangrove samplings in Kendari Beach, where seven species found at site I and III, whereas six in found at site II. Species of Littorina scabra showed as the heighest frequency value at all site is 0,75. Therefore, the Littorina scabra are the common snails in this region, bacause they were found at all site in Kendari Beach. The abundance of gastropods in the mangrove sampling was found to be high at roots and has the lowest at leaves. The species of Littorina scabra at all vegetative organs of mangrove samplings in the Kendari Beach and suggesting is widest distribution.Keywords : Gastropods, Abundance, Vertical Distribution, Bay of Kendari.
Uji Kepekaan Kulit Batang Ceremai (Phyllanthus acidus L.) terhadap Pertumbuhan Eschericia coli. Sernita Sernita
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 3, No 2 (2016): Biodiversity in Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.332 KB) | DOI: 10.33772/biowallacea.v3i2.3233

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui kepekaan kulit batang ceremai (phyllanthus acidus L.) dan untuk mengukur diameter zona hambat kulit batang ceremai  terhadap pertumbuhan Escherichia coli ATCC 35218. Sampel dalam penelitian ini adalah kulit batang ceremai yang diekstraksi dengan metode maserasi menggunakan etanol 96%. Dibuat pengenceran dengan konsentrasi 20%, 40%, 60%, 80% dan 100%. Pengujian kepekaan menggunakan media NA dengan metode cawan kertas. Biakan bakteri dari media NB dicampur dengan media agar, kemudian cawan kertas diletakkan di atas media agar. Diinkubasi pada suhu 37oC selama 48 jam. Diukur zona hambat menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa kulit batang ceremai konsentrasi 20% tidak dapat menghambat pertumbuhan bakteri Escherichia coli. Pada konsentrasi 40% rata-rata diameter zona hambat 4,03 mm. Pada konsentrasi 60% rata-rata diameter zona hambat 4,36 mm. Pada konsentrasi 80% rata-rata diameter zona hambat 5,56 mm. Dan pada konsentrasi 100% dapat menghambat pertumbuhan bakteri Escherichia coli secara optimal dengan rata-rata diameter zona hambat 7,03 mm. Kata Kunci : Escherichia coli, ceremai, Nutrient Agar ABSTRACTThis study aims to determine the sensitivity of the bark ceremai (Phyllanthus acidus L.) and to measure the diameter of inhibition zone ceremai bark on the growth of Escherichia coli ATCC 35218. The sample in this study is the bark ceremai extracted by maceration method using ethanol 96%. Created dilution with a concentration of 20%, 40%, 60%, 80% and 100%. NA sensitivity testing using media with paper plate method. NB bacterial culture media mixed with agar medium, then the paper cup is placed on an agar medium. Incubated at 37 ° C for 48 hours. Inhibition zone was measured using a caliper. The results show that the research is conducted bark ceremai concentration of 20% could not inhibit the growth of bacteria Escherichia coli. At a concentration of 40% average inhibition zone diameter of 4.03 mm. At a concentration of 60% average inhibition zone diameter of 4.36 mm. At a concentration of 80% average inhibition zone diameter of 5.56 mm. And at 100% concentration to inhibit the growth of bacteria Escherichia coli optimally with an average diameter of 7.03 mm zone of inhibition. Keywords : Escherichia coli, ceremai, Nutrient Agar
DETEKSI PENYAKIT HEPATITIS-B PADA IBU HAMIL DI PUSKESMAS ABELI KOTA KENDARI Susanti Susanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.43 KB) | DOI: 10.33772/biowallacea.v4i1.3238

Abstract

ABSTRAKPenyakit Hepatitis B merupakan peradangan atau infeksi pada sel-sel hati yang disebabkan oleh virus Hepatitis B.  Penularan virus Hepatitis B melalui darah atau cairan tubuh yang mengandung virus Hepatitis B.  Penyakit Hepatitis B dapat dideteksi salah satunya dengan pemeriksaan HBsAg (Hepatitis B Surface Antigen) yang merupakan antigen permukaan dari Virus Hepatitis B. Ibu hamil yang terinfeksi virus Hepatitis B di Indonesia berkisar antara 1-5 %. Infeksi virus Hepatitis B pada ibu hamil dapat mengakibatkan hepatitis fulminan dan meningkatkan mortalitas pada ibu dan bayi. Data yang diperoleh di Puskesmas Abeli Kota Kendari, ibu hamil yang datang berkunjung ke puskesmas Abeli pada bulan Januari-Maret 2017 sebanyak 133 orang, dan tidak terdapat data pemeriksaan Hepatitis B pada ibu Hamil. Tujuan penelitian ini adalah untuk mendeteksi penyakit hepatitis B pada ibu Hamil di Puskesmas Abeli kota Kendari. Jenis penelitian yang digunakan adalah deskriptif observasional, dengan metode cross sectional dan pengambilan data secara Accidental sampling, sampel diperoleh dari pasien ibu hamil yang datang ke Puskesmas Abeli selama penelitian dilakukan. Jumlah sampel penelitian yang dilakukan pemeriksaan Hepatitis B pada ibu Hamil di Puskesmas Abeli sebanyak 25 orang. Hasil penelitian diperoleh sampel positif Hepatitis B sebanyak 1 orang (4%) dan sampel negative Hepatitis B sebanyak 24 orang (96%).  Kesimpulan hasil deteksi Hepatitis B pada ibu Hamil di Puskesmas Abeli kota Kendari menunjukkan terdapat hasil positif Hepatitis B pada ibu Hamil sebanyak 1 orang (4%) dan hasil negative Hepatitis B pada ibu Hamil sebanyak 24 orang (96%). Kata Kunci : Hepatitis-B, Ibu Hamil, Deteksi ABSTRACTHepatitis B is an inflammation or infection of liver cells caused by Hepatitis B virus. Hepatitis B virus transmission through blood or body fluids containing Hepatitis B virus. Hepatitis B disease can be detected by HBsAg examination (Hepatitis B Surface Antigen) which is the surface antigen of Hepatitis B Virus. Pregnant women infected with Hepatitis B virus in Indonesia ranges from 1-5%. Hepatitis B viral infection in pregnant women can lead to fulminant hepatitis and increase maternal and infant mortality. Data obtained at Puskesmas Abeli Kendari, pregnant women who come to Abeli health center in January-March 2017 as many as 133 people, and there is no data of hepatitis B examination in pregnant women. The purpose of this study was to detect hepatitis B disease in Pregnant women at Abeli Puskesmas Kendari. The type of research used is descriptive observasional, with cross sectional method and data collection by Accidental sampling, sample obtained from pregnant woman patient coming to Puskesmas Abeli during research done. The number of samples of research conducted Hepatitis B examination in Pregnant Women at Puskesmas Abeli as many as 25 people. The result of the study was Hepatitis B positive 1 (4%) and Hepatitis B negative sample 24 (96%). The conclusion of result of Hepatitis B detection in pregnant mother at Abeli Kendari health center showed positive result of Hepatitis B in pregnant mother as much as 1 person (4%) and negative result of Hepatitis B in pregnant mother counted 24 people (96%). Keywords: Hepatitis B, Pregnant Women, Detection
Seleksi Material Penempelan Biofilm Isolat Bakteri Resisten Tembaga asal PT. Freeport Indonesia Maria Massora; Erni Martani; Eko Sugiharto; Roberth Sarworm; Tumpal Sinaga
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.647 KB) | DOI: 10.33772/biowallacea.v4i1.3273

Abstract

ABSTRAK Bakteri yang mampu tumbuh pada lingkungan tercemar logam berat cenderung membentuk biofilm. Biofilm terdiri dari berbagai kelompok bakteri yang tumbuh bersama mikroba lain yang diselubungi matriks polimer ekstraseluler (EPS) dan  melekat pada permukaan materi organik maupun anorganik. Empat isolat bakteri  yang digunakan dalam penelitian ini adalah bakteri resisten tembaga yang  diisolasi dari tailing PT Freeport Indonesia, Timika, Papua. Pada penelitian ini dilakukan seleksi materi penempelan biofilm berupa materi organik maupun anorganik untuk memperoleh materi penempelan yang optimal untuk pertumbuhan biofilm. Masing- masing isolat diinokulasikan ke dalam medium LB yang mengandung 100 mg/L CuSO4. Material uji digantung pada tutup erlenmeyer, diinkubasi pada shaker inkubator dengan kecepatan 100 rpm, selama 2 minggu pada suhu ruang. Aktivitas pembentukan biofilm diamati setiap minggu sekali berdasarkan penempelan biofilm pada material yang diamati di bawah mikroskop. Pembentukan massa biofilm diukur dengan cara menimbang material penempelan sebelum dan sesudah  penempelan biofilm.  Empat isolat dapat tumbuh dengan baik pada batu kali, kayu, plastik LLDPE dan plastik PET namun penempelan biofilm lebih stabil pada potongan kayu dan batu kali. Pseudomonas aeuruginosa strain C53 menunjukkan kemampuan paling tinggi dalam pembentukan biofilm kemudian diikuti Bacillus cereus strain C38, Lycinibacillus fusiformis strain C40 dan Bacillus subtilis strain C43. Kata Kunci : Bakteri Resisten tembaga, Biofilm, Material Penempelan. ABSTRACTBacteria capable of growing in a heavy-metal polluted environment tend to form biofilm. Biofilm consists of various groups of bacteria that grow together with other microbes that are surrounded by the extracellular polymeric substance  (EPS) and adhere to the surface of both organic and inorganic matter. Four bacterial isolates used in this study were copper-resistant bacteria isolated from PT Freeport Indonesia's tailings, Timika, Papua. In this research, selection of biofilm attachment material was conducted in the form of organic or inorganic material to obtain optimal attachment material for biofilm growth. Each isolate was inoculated into LB medium containing 100 mg/L CuSO4. The test material was suspended on the erlenmeyer cover, incubated in the incubator shaker with 100 rpm, for 2 weeks at room temperature. The biofilm forming activity was observed weekly based on the attachment of biofilms to the material observed under the microscope. To determine the mass of the biofilm formed, calculation of the attachment material weight was done before and after the biofilm attachment. Four isolates could grow well on stone, wood, LLDPE plastic and PET plastic. However, the biofilm attachment looked more stable on wood and stone fragments. The highest ability in the biofilim formation was performed by Pseudomonas aeuruginosa strain C53, followed by Bacillus cereus strain C38, Lycinibacillus fusiformis strain C40 and Bacillus subtilis strain C43. Keywords : Copper Resistant Bacteria, Biofilm, Attachment Material
KOMPOSISI DAN KEANEKARAGAMAN JENIS TUMBUHAN GOLONGAN POHON DI KAWASAN HUTAN LINDUNG NANGA-NANGA PAPALIA KOTA KENDARI SULAWESI TENGGARA Muhsin, Muhsin; Indrawati1, Indrawati; Rahardi, Wira
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
Publisher : Jurnal BioWallacea

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this research was to know the composition, diversity and distribution ofdiameter class (vertical structures) of plant on tree categories in Protected Forest Area ofNanga-Nanga Papalia, Kendari Town, Southeast Sulawesi Province. This research wasdone by using 5 terraced transects, which was laid followed the river stream by consideringthe elevation place and vegetated condition. Each transect consist of 5 observed plots whichwere laid systematically along the transect line. Variables observed was the composition anddiversity of plant species by the growth stager including tree, pole, sapling, seedling andstand diameter. Ecological indicators of plants were analysed including number of species,density, relative density, frequency, relative frequency, dominancy, relative dominancy,important value index, diversity index, evennes index and stand diameter. The result showedthat were discovered about 53 species of plants on tree categories, which were classifiedinto 27 families and were distributed by the growth stager, i.e. 31 species of tree, 42 speciesof pole, 31 species of sapling and 26 species of seedling. Metrosideros petiolata had thehighest important value index for the growth stager tree and pole (54,474% and 62, 516%),while Baringtonia racemosa had the highest important value index for growth stager saplingand seedling (36,361% and 31,504%). The diversity index was in estimated grade for treeand seedling (2,908 and 2,529), while the diversity index was in high grade for pole andsapling (3,102 and 3,032). The distribution of stand diameter of plants in Protected ForestArea of Nanga-Nanga Papalia showed a balanced community which was dominated bytrees.Key words : Stand Diameter, Tree Categories, Protected Forest Area, Diversity,Composition.
STUDI KARAKTERISTIK MIKRO-HABITAT BURUNG MALEO (Macrocephalon maleo) PADA KAWASAN TAMAN NASIONAL RAWA AOPA WATUMOHAI (TNRAW) SULAWESI TENGGARA Jamili, Jamili; Analuddin, Analuddin; Rudia, Adi Parman Parman
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
Publisher : Jurnal BioWallacea

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study was to determine the characteristics of the bird nestingmicrohabitat hole Maleo (Macrocephalon maleo) in National Parks Aopa WatumohaiSwamp, Southeast Sulawesi . Data were collected on the savanna region block MempahoForest and savanna region Pampaea , by using descriptive method to determine the depth,temperature, pH and substrate nesting holes were found during the study . To determine thecomposition of the vegetation around the nesting hole , using roaming with 10 meters toexplore the area around the hole nesting and record the type of vegetation found. Vegetationtypes have been known to direct scientific name recorded in the field. While the type ofvegetation that is unknown scientific name, a swab or documentation then be describedfurther in the Laboratory of Ecology and Natural Sciences Taxonomy Halu Oleo Universitywith reference to the book (FLORA by Steenis, et al., 1997). The results showed the depthof hole nesting birds Maleo (Macrocephalon maleo) in the study area is 50-60 cm,temperature 28o-32oC, and soil pH of 5.9 - 7. Maleo bird nesting substrate type is dominatedby sand. Types of vegetation found around the hole nesting nesting is Melastoma sp. andKirinyuh (Eupathorium sp.), while the type of vegetation that is a place to find food andshelter includes a thorn Bamboo (Bambusa spinosa), Rao (Dracontomelon mangiferum),Banyan (Ficus spp.), Tamarind (Aleurites molucana), Caesalpinia pulcherrima, forest Mango(Mangifera sp.), Kuia (Alstonia scolaris), Nona (Metrosideros petiolata), and Bitti/Kulipapo(Vitex sp.). Total current Maleo birds encounter is as much one of the males and femaleslaying eggs on the location of the savanna region Pampaea Resort Langkowala SwampNational Park area of Rawa Aopa Watumohai Southeast Sulawesi .Keywords : Characteristics microhabitat, Bird Maleo (Macrocephalon maleo), Rawa AopaWatumohai National Park Southeast Sulawesi.
KELIMPAHAN COLLEMBOLA PERMUKAAN TANAH DI DAERAH PERKEBUNAN MASYARAKAT DI DESA LALOWIU KECAMATAN KONDA KABUPATEN KONAWE SELATAN PROVINSI SULAWESI TENGGARA Suriana Suriana; Amirullah Amirullah; Wahyuni Wahyuni
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 2, No 2 (2015): Keragaman dan Bioteknologi
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v2i2.1467

Abstract

The aim of this study is to know the abundance of ground surface Collembolan in plantation area at Lalowiu villlage subdistrict of Konda, South Konawe regency, province of Southeast Sulawesi. The research was done by observation a the ground and sample collection by using pitfall trap and litter trap. Trap settelment at 09.00 pm and sample collecting at 09.00 am 3 days after. Litter trap sample collection was done concurrent with pitfall trap sample collection. Extract of litter trap sampling conducting by using Berlese funnel. Result of the research show that 3 ordo which divided in 6 family and 16 genus. The highest abundance of collembola at Homidia genus 8,1 Ind/m2 and the lowest abundance at Sphaeridia genus 0,05 Ind/m2.Keywords : Collembola, , Berlese funnel, Lalowiu Village, The Abundace, Pittfall trap
DENSITAS COLLEMBOLA DALAM TANAH PADA PERKEBUNAN KAKAO DI DESA POLEONRO KECAMATAN POLEANG TENGAH KABUPATEN BOMBANA SULAWESI TENGGARA Sitti Wirdhana Ahmad; Amirullah Amirullah; Ranti Melkaresi
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 2, No 2 (2015): Keragaman dan Bioteknologi
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.161 KB) | DOI: 10.33772/biowallacea.v2i2.1478

Abstract

ABSTRACT The aim of this research was to know the number of the family and the density of Collembolan found in the cocoa plantations in the village of Poleonro, Districts Poleang, Bombana, Southeast Sulawesi. This studied was of research exploration. The sampling done with the quadrant method is to install a number of quadrants in the specified path along 100 meters. Sampling was carried out above ground level and sampling under the ground level. The extration of Collembola was done used by Tullgreen Tunnel. The results showed that the cocoa in the village in cocoa plantations in the village poleonro namely Entomobrydae, Aronelidae, Tomoceridae, Willowsinae, Paronelidae, Oncopoduridae, Isotomidae, Cyphoderidae dan Arropalitidae, the density Collembola found as mus as 2 of the order that are divided into 9 families. Higest density on the family of Paronellidae (15,06%) and followed by family of Willowsinae and Arropalitidae (8,15%). Keywords: Density, Collembola, Cacao plantations, Village of Poleonro
PENGARUH REBUSAN GAMBAS (Luffa cilindrica) TERHADAP KADAR GLUKOSA DARAH DAN PROFIL LIPID PADA TIKUS WISTAR DIABETES Barinta Widaryanti; Arshita Eka Kurniawati; Nuriansyah Putri
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 3, No 1 (2016): BIodiversitas
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/biowallacea.v3i1.1481

Abstract

ABSTRACT Diabetets mellitus is a common desease which is caused by insulin secretion and action disorder. Prevelence of the desease was predicted to be increase 2,5 times in 2030, therefore, special treatment were needed to improve the quality of life diabetic patient. Recently, many natural drug has been developed for diabetic therapy. The objective of this research was to investigate the effect of boiled sponge gourd in reducing blood glucose dan lipid profile on male diabetic rats alloxan induced. Eighteen diabetic male wistar rats were divided into three groups, first grup were normal rats, second gorup were diabetic rats,and third gorup were diabetic rats that were treated with boiled sponge gourd for fourtheen days. The research showed that boiled sponge gourd was able to reduce blood glucose, total cholesterol and triglyceride. Keywords : Diabetes mellitus, gambas, anti diabetic drug, alloxan

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