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INDONESIA
Majalah Kedokteran Gigi Indonesia
Published by Universitas Gadjah Mada
ISSN : 24600164     EISSN : 24422576     DOI : https://doi.org/10.22146/majkedgiind.36959
Core Subject : Health,
Dentistry
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 5, No 3 (2019): December" : 8 Documents clear
Effect of nano silica coating concentration on reducing acrylic resin denture cytotoxicity Gene Rizky Natalia Gunawan; Titik Ismiyati; Haryo Mustiko Dipoyono
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37166

Abstract

Acrylic resin is a denture base material mostly used in the fabrication of removable partial denture and complete denture because it is easy to process, economical and aesthetically satisfying. However, the acrylic resin has a weakness i.e. residual monomer content which causes cytotoxic effect to human cells. Nano-silica coating could be used on the acrylic resin to reduce residual monomer content. The purpose of this study was to determine the effect of nano-silica coating concentration on the cytotoxicity of acrylic resin denture base material. Twenty-four acrylic resin disc-shaped specimens (5 mm in diameter and 2 mm in thickness) were divided into 4 groups (n=6), i.e. control, group with application of 0.5%,1%, and 2% nano silica coating. The specimens on the silica coating groups were silica coated using dip-coating method. After the coating was completed, the specimens were added into cell fibroblast culture (vero cell line). Cytotoxicity testswere done by MTT assay. The optical density was measured using ELISA plate reader to determine the percentage of cell viability. The highest mean of cell viability was shown in 0.5% concentration (96.78 ± 1.71) and the lowest mean was shown in the control group (80.32 ± 6.53). One-way ANOVA showed that there were significant differences among these groups. LSD tests showed that there was significant difference among all of the groups. This study concluded that nano silica coating concentration has a significant effect on the cytotoxicity of acrylic resin denture base materials.
Potency of stored DNA derived from buccal mucosal epithelial cells for genotype analysis of interleukin 4 -590 C/T Rezmelia Sari; Alya Nur Fadhilah; Prayitno Prayitno
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37361

Abstract

The genotype variation of the interleukin 4 (IL-4) gene on spot -590 C/T is known to correlate with IL-4 protein expression in periodontitis, but the results found in the population are inconsistent. Sequencial genotype analysis of multiple genes often used stored DNA samples obtained from buccal mucosal epithelial cells. This study aims to determine the use of stored DNA samples obtained from buccal mucosal epithelial cells for genotype analysis specifically on -590 C/T IL-4 genes. This is a descriptive analytical study. The samples were 58 DNA derived from the buccal mucosal epithelial cells taken by sterile cotton swab isolated with PrestoTM kit (GeneAid). The DNA has been stored for 2 years at -20 °C. The genotype analysis was conducted using PCR RFLP technique with BSmFI enzyme and the data were presented descriptively. The results show that 51.72% of the stored DNA is still in good condition and 37.9% can be used for genotype analysis. It was concluded that DNA derived from buccal mucosal epithelial cells stored at -20 °C for 2 years can still be used for genotype analysis, but the quality of DNA is affected by storage time. 
Effect of sandwich bone augmentation using hydroxyapatite and demineralized freeze- dried bone on infrabony pocket treatment Eka Pramudita Ramadhany; Al Sri Koes Soesilowati; Sri Pramestri Lastianny
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37427

Abstract

Periodontitis is periodontal inflammation in response to plaque bacterial antigens, causing damage to periodontal ligament and alveolar bone resorption. Bone graft material combination i.e. demineralized freeze-dried bone allograft (DFDBA) and hydroxyapatite (HA) using sandwich bone augmentation (SBA) method will support each other and will be beneficial to be used as a scaffold. The body takes long time to resorb HA so this could complement DFDBA which is more easily dissolved. This study aimed to reveal the effect of bone graft addition using SBA method on the treatment of infrabony pocket with open flap debridement in terms of probing depth, relative attachment loss, alveolar bone height, and density. This study was carried out to 20 infrabony pockets, where 10 of them were treated using open flap debridement with HA addition, while the other 10 groups were treated using open flap debridement with DFDBA and HA using SBA method. Probing depth  and relative attachment loss were measured on days 0, 30 and 90. Bone height and density were measured using cone-beam computed tomography (images on day 0 and 90). The study showed that probing depth reduction on SBA group was greater than HA group. There were significant differences in probing depth and relative attachment loss examinations. However, bone height and bone density reduction did not show any significant difference. The conclusion from this study is open flap debridement using SBA method yields better regeneration in terms of probing depth and relative attachment loss than open flap debridement with HA addition. There is no difference in bone height and bone density between the two groups.
Histopathological characteristics of dental socket healing on collagen density following use of pangas catfish (Pangasius djambal) gelatin Fredy Mardiyantoro; Nenny Prasetyaningrum; Hani Tri Rahmastuti
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.39830

Abstract

Tooth extraction is a common treatment in dental practice. However, complications such as bleeding or dry socket may occur during healing process. Pangas catfish (Pangasius djambal) gelatin contains glutamine which could increase the proliferation of fibroblast, accordingly collagen synthesis increases. The study aimed to demonstrate collagen density after the application of pangas catfish gelatin on post-tooth extraction wound of albino rats (Rattus norvegicus). This wasan experimental study with randomized posttest-only control group design which involved male Wistar strain albino rats. The rats were selected using a random sampling method and divided into four groups, namely day-3 control group (n=7),day-7 control group (n=7), day-3 treatment group (n=7) and day-7 treatment group (n=7). Pangas catfish gelatin was administered into the socket once after extraction. The variable measured in the study was collagen density, determinedby collagen scoring of hematoxylin eosin–stained histopathological preparations. Collagen formation were determined according to the assessment criteria of collagen distribution and density. Data analysis showed there was a significantdifference of collagen synthesis between the control and treatment groups according to Kruskal-Wallis test of which the result of control group day-3 was 1.13 ± 0.34, control group day-7 was 1.76 ± 0.67, treatment group day-3 was 1.66 ±0.47 and treatment group day-7 was 2.93 ± 0.25. In addition, the day-7 treatment group showed the highest collagen scoring values as compared to the control groups according to the Mann-Whitney test (p<0.05). In conclusion, pangascatfish gelatin increased collagen density during dental socket healing process after tooth extraction in albino rats.
β-Carotene patch application effects on gingival crevicular fluid volume after panoramic radiography exposure Anggun Dwi Andini; Rini Widyaningrum; Rurie Ratna Shantiningsih
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.42486

Abstract

Panoramic radiography is a radiography technique which is commonly used in dentistry. X-ray radiation from panoramic radiography can cause biological damage, such as the increase of gingival crevicular fluid (GCF) volume. One of materials which was known having the ability to decrease the effect of panoramic radiography is β-carotene in mucoadhesive gingival patch form. The application of β-carotene mucoadhesive gingival patch reduce micronucleus increasing in previous study. The aim of this study is to determine the effect of β-carotene mucoadhesive gingival patch application on palatal gingiva of maxillary incisor teeth to the GCF volume of panoramic radiography exposed patients. This study involved 10 subjects (5 females and 5 males). The GCF sample was taken from the labial sulcus of 11, 12 teeth (treatment group) and 21, 22 teeth (control group) of each subject before and after panoramic radiography exposure. β-carotene mucoadhesive gingival patch was applicated on palatal gingiva before panoramic radiography examination. Sample was collected using an absorbent paper which is inserted to the labial gingival sulcus. The GCF on the absorbent papers were marked by adding ninhydrin 2%, then the GCF volume was measured using a sliding caliper. Paired t-test result shows a significant difference between GCF volume in each group before and after panoramic radiography exposure (p<0.05). Independent t-test shows no significant difference between the GCF volume difference in the control and treatment group (p>0.05). The result of this study shows that the application of β-carotene mucoadhesive gingival patch on palatal gingiva of maxillary incisor teeth shows insignificant effect in decreasing the increase of GCF volume in panoramic radiography exposed patients.
The differences in parallelism between ala-tragus line and occlusal plane of down syndrome and normal individuals An-Nissa Kusumadewi; Erna Kurnikasari; Rasmi Rikmasari; Willyanti Soewondo
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.42488

Abstract

People with down syndrome have a different craniofacial structure compared to normal individuals. Commonly, down syndrome patients have abnormal ear morphology with smaller size, and lower ear position compared to that of normalsubjects. These patients also have smaller nose, shorter maxilla structure and facial height. Other disorders are anomalies in tooth shape, numbers and size. The ala-tragus line is one of the anatomical landmarks to determine the occlusal plane orientation. Abnormalities in the anatomical structure of people with down syndrome can affect the parallelism between the ala-tragus line and the occlusal plane. The purpose of this research was to determine the differences in parallelism between the ala-tragus line and the occlusal plane of people with down syndrome and normal individuals. The sample in this study were people with down syndrome and normal individuals. The sample was photographed using a digital camera with a fox plane attached to the oral cavity. Parallelism of the ala-tragus line and the occlusal plane was analyzed by tracing these photographs. Photo tracing was done using the Autocad 2013 software. The mean value of angle on people with down syndrome (5.852° ± 5.367°) was greater than that of normal individuals (2.169° ± 2.557°). Based on the research, it was concluded that the parallelism of the ala-tragus line to the occlusal plane in people with down syndrome was different from that in normal individuals. The ala-tragus inferior line in normal individuals was more parallel to the occlusal plane compared to that in people with down syndrome.
Local application of biphosphonate as a biochemical barrier against bone graft resorption in mandibular reconstruction Romzi Hanif; Muhammad Masykur Rahmat; Cahya Yustisia Hasan
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.45096

Abstract

Mandibular reconstruction to restore functions and aesthetics after mandibular resection remains a major surgical challenge. This type of reconstruction using non-vascularized autogenous bone graft harvested from iliac bone has been popular given that this bone is numerous and donor site morbidity can be minimized, however, it is highly resorbable. Local application of bisphosphonate by immersing bone graft in biphosphonate manages to inhibit bone graft resorption prior to the formation of new bone to support the osteogenesis and osteointegration of bone grafts. This paper aimed at examining the positive results of iliac bone graft osteogenesis and osteointegration following local application of bisphosphonate in mandibular reconstruction. A 22-year-old female patient came with a complaint of painless right cheek mass that has swollen slowly since 2 years. Radiographic examination showed cloudy radiolucent images in the right mandibular corpus to the right mandibular ramus, while histopathological examination indicated ossifying fibroma. Patient underwent mandibular resection followed by iliac bone graft-based mandibular reconstruction. Bone graft was immersed with bisphosphonate (zoledronate acid 0.005 mg /ml) for 3 minutes, then rinsed with saline for 3 minutes, followed by fixing bone graft on the reconstruction plate. Postoperative follow up in the 36th week showed no signs of infection and dehiscence in the surgery site, and the radiographic examination indicated signs of osteogenesis and osteointegration of mandibular bone graft. Local application of biphosponate on bone graft promotes favorable results of osteogenesis and osteointegration in mandibular reconstruction.
Oral health knowledge and practices of antenatal mothers in Hospital Universiti Sains Malaysia Muhammad Azim Syahmi Kamaruddin; Norsamsu Arni Samsudin
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.45958

Abstract

Mothers play a very significant role in influencing the oral health status of the family. Many studies have highlighted the importance of good oral health during pregnancy. This study aimed to assess knowledge, self-perceived oral health status, and practices of antenatal mothers in Hospital Universiti Sains Malaysia, Kelantan, Malaysia. A total of 76 antenatal mothers visiting Obstetrics & Gynaecology specialist clinic at Hospital USM were involved in this cross-sectional survey study. The questionnaires contained items related to oral health knowledge, self-perceived dental problems, barriers to seeking dental care, oral hygiene habits, perceptions of oral health, and access to dental care. Majority (98.7%) agreed that their oral health was important as part of general health. About 58% of them had answered correctly regarding oral health knowledge. Most respondents had self-perceptions of having dental problems during pregnancy, including cavitated teeth (34.4%) and sensitive teeth (20.5%). Regarding practice, only 35.3% visited a dentist in the last six months. Time constraints (52.4%) and safety concerns regarding dental treatment (26.2%) were the main barriers to seek dental care. Many (90%) brushed their teeth at least twice daily with adult fluoridated toothpaste, while some used mouthwash daily (36.8%) and flossed their teeth (11.8%). Antenatal mothers had an average level of knowledge regarding oral health, experienced dental problems during pregnancy and lacked awareness of a regular dental visit. Hence, there is a need for more vigorous oral health promotion, which also include antenatal care providers, to improve oral health awareness among antenatal mothers.

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