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Ivan Permana Putra
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Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
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Jurnal Sumberdaya Hayati
Published by Institut Pertanian Bogor
ISSN : -     EISSN : 2477037X     DOI : DOI: 10.29244/jsdh.9.1.7-16
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
The "Jurnal Sumberdaya HAYATI" is a peer-reviewed and an open access journal published by Department of Biology, Bogor Agricultural University, Indonesia. The journal publishes significant and important research from all area of biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 4 No. 2 (2018)" : 5 Documents clear
Genetic Variation Analysis of Hevea brasiliensis Genotype Population of In Vitro Micro-Cutting Culture by RAPD Marker MARTIANSYAH, IRFAN; HARIS, NURHAIMI; HUSNIYATI, TATI; PURWAKUSUMAH, EDI DJAUHARI
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.57-62

Abstract

The rubber seeds are insufficient for producing rootstocks to rubber grafting. It can be overcome by an in vitro micro-cutting culture technique developed in the Indonesian Research Institute for Biotechnology and Bioindustry (IRIBB). However, the origin clone of 57 rubber genotypes used as an explant source in vitro micro-cutting culture is not recognized. The study was to investigate the 57 genotypes that came from mixed GT 1, PB 260, and RRIM 600 as parent clones. We investigated using seven primers of Random Amplified Polymorphic DNA (RAPD), i.e., OPA 02, OPA 07, OPA 15, OPB 04, OPC 05, OPC 11, and OPC 20. The qualitative analyzed by electrophoresis 1% gel agarose. A total of 47 DNA fragments produced with an average of 7 fragments per primer. OPA 02 generated of 13 fragments, whereas OPB 04 only one fragment. The DNA fragment pattern shows the presence of polymorphism. The genetic similarity coefficients obtained in the range of 62-96%. The highest genetic similarity (96%) is genotype 70 and 78. It recognized that 42 genotypes from 57 rubber genotypes had the closest relationship with PB 260 clones. Furthermore, six genotypes had a significant growth response as an explant in vitro micro-cutting culture.
Profil DNA Forensik pada Barang Bukti Dua Kasus Pembunuhan di Indonesia RIANTI, PUJI; CRISTIN, ELISA; WIDODO, PUTUT TJAHJO
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.48-56

Abstract

DNA technology for profiling purposes has been used in many basic and applied science. One of the emerged applied science in genetics is it’s uses in solving crime cases. Homicide became one of highest crime cases in Indonesia. Solving its cases through DNA profiling technology using items of evidence as tool is needed. Here, we report the profiling of human DNA from several items of evidence available in the crime scene and the suspect. We used items of evidence from study cases no. 18098 and 18101, based on the legal permission of Indonesia’s National Police. We used 21 international standards of human STR markers, one sex-determining marker, one Y STR marker, and one independent Y marker to developed human alleles from tissue and blood stains left and/or shred on the victims, soap bottles, knifes, victim’s clothes and ropes as well as the buccal swab of the crime suspect. Our alleles identification matched between the victim and the crime suspect in both cases with the accuracy of DNA profiles compatibility at 99.99%. Detection of DNA profiling is depending on the evidence and time of storage which are influence by environment that can lead to the process of decayed and/or contaminated.
Additional nest structures and natural enemies of stingless bees (Hymenoptera: Apidae: Meliponinae) PANGESTIKA, NORITA WIDYA; ATMOWIDI, TRI; KAHONO, SIH
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.42-47

Abstract

Stingless bees (Hymenoptera: Apidae) are widely distributed in tropics and subtropics areas. Now these bees are farmed by the human because they produce honey and propolis. Natural enemies can disturb the colony that affects to nest structure and productivity of these bees. This study aimed to study the common nest structure and additional nest structure of stingless bees which caused by natural enemies. This research was conducted from August to November 2015. Samples were taken from three locations, i.e, Pasuruan (East Java), Tasikmalaya (West Java), and Rangkasbitung (Banten). Descriptive analysis was used to compare the structure and composition of the nest in bamboo and additional nest structures in relation with natural enemies. Generally, the structure and composition of the nest of stingless bees in bamboo consist of nest entrance, storage pots (honey and pollen), and brood cells. We didn’t found natural enemies of stingless bees in Pasuruan and Tasikmalaya and these colonies did not create additional nest structures. In contrast, the colonies in Rangkasbitung created additional nest structures i.e. the inner entrance covered by batumen and cerumen and also build a root-like structure. Natural enemies found in the colony at Rangkasbitung were cockroaches (Blattodea: Blattidae), histerid beetles, Platysoma leconti (Coleoptera: Histeridae), nitidulid beetles, Carphophilus sp. (Coleoptera: Nitidulidae), and ants, Pheidole sp. (Hymenoptera: Formicidae).
Aplikasi Trigona (Tetragonula) laeviceps sebagai Agen Penyerbuk pada Sistem Tumpang Sari Buncis dan Tomat di dalam Rumah Kaca LEKSIKOWATI, SOVIA SANTI; EKA PUTRA, RAMADHANI; ROSMIATI, MIA; KINASIH, IDA; ZAKIYATUN USNA, INAYATI; NOVITASARI; SETYARNI, EKA; ANJARNISA RUSTAM, FAJRINA
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.63-70

Abstract

Greenhouse is widely applied to provide protection against pest and harsh environment condition and most farmers usually planted mixed crops inside. However, this system creates an isolation to pollination which responsible for fruit production. In this study, local domesticated stingless bees, Trigona (Tetragonula) laeviceps, was applied as pollinator for two crops, beans (Phaseolus vulgaris L.) and tomatoes (Solanum lycopersicum L.) planted as mixed crops inside greenhouse. The benefit of the bees was measured as pollination success and quality of the crops produced (size and weight). Tomato flowers received no visit from both wild pollinators (all was Apis cerana) and stingless bees while in bean flowers, A. cerana exhibited more and longer visits to flower than T. laeviceps albeit lower number of flowers visited. The pollination efficiency between wild pollinator and T. laeviceps for beans (both at 41%) while self pollination produced better pollination efficiency for tomatoes planted at open field (84% compared to 71%). Beans planted at open field produced better products (bigger size and heavier) while tomatoes produced lesser quality although the differences were not significant. Based on this study, T. laeviceps may applied as pollinator agent in greenhouse and for mixed crops of beans and tomatoes.
Daerah Jelajah dan Penggunaan Ruang Kukang Jawa (Nycticebus javanicus) Di Taman Nasional Gunung Halimun Salak, Jawa Barat ARISMAYANTI, EKA; PERWITASARI, RR DYAH; WINARTI, INDAH
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.28-41

Abstract

Javan slow loris (Nycticebus javanicus) is a slow moving nocturnal prosimian primate. Javan slow lorises are considered critically endangered by International Union for the Conservation of Nature. To date, there is limited source information available regarding wild loris ecology and behavior. In this study, home range areas and use space of wild Javan slow loris was carried out in Mount Halimun-Salak National Park. This study aims to measure home range size and to analyze activity budgets in natural forest conditions. Kernel Home Range methods were used to analyze home range areas, while Point Centered Quarter methods were used to collect habitat data, and five minute Instantaneous-Focal Animal Sampling was used to observe and record behavioral data. Two adult female lorises were observed. Analysis showed contiguous home range sizes of 5.43 and 5.58 hectares for Individuals I and II, respectively. These home ranges were larger than that reported in Malaysian Nycticebus coucang. Behavioral analysis showed that daily activities were dominated by travel, feeding, active, and foraging, which took place primarily in the lower canopy stratum, whereas sleeping typically occurred in the medium canopy stratum. The use of space of Javan slow loris correlated with height, substrat, contact, and size of branch.

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