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Chromium (Cr) Metal Absorbtion Using Symbiosis of Fimbristylis globulosa with Agrobacterium sp. Alfian Chrisna Aji; Novia Citra Paringsih; Dwi Rizaldi Hatmoko; Ilham Mujahidin; Riza Rifai; Afif Iqbal Alviandi; Almas Al Jinan; Mujahidin Mujahidin; Sofyan Anif
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.16213

Chromium (Cr) metal is a pollutant caused by industrial waste disposal and agricultural activities. An alternative solution to managing chromium pollution in an environmentally friendly manner is phytoremediation using Fimbristylis globulosa combined with Agrobacterium sp. This research aims to measure 1). the ability of Fimbristylis globulosa combined with Agrobacterium sp. to absorb Cr metal; and 2). the value of the Cr metal bioconcentration factor (BCF) absorbed by Fimbristylis globulosa combined with Agrobacterium sp. This research used factorial design 2x5 with randomized design. Chromium metal analysis using atomic absorption spectrophotometer. Data were analyzed using BCF test. The results showed: 1) the highest Cr metal absorption in the treatment of Fimbristylis globulosa-Agrobacterium sp.-Cr metal content of 30 ppm (T2V3) root: 7,987 ppm and shoot: 15,649 ppm; 2) the highest value of Cr metal BCF in the treatment of Fimbristylis globulosa-Agrobacterium sp.-Cr metal content of 30 ppm (T2V3) root is 0.266 and Fimbristylis globulosa-Agrobacterium sp.-Cr metal content 10 ppm (T2V1) shoot is 0.675. Conclusions of this research are: 1) Fimbristylis globulosa combined with Agrobacterium sp. has a higher Cr metal absorption ability than without a combination of both; and 2) Fimbristylis globulosa combined with Agrobacterium sp. has a higher value of the Cr metal BCF than without a combination of both.

Isolation and Molecular Identification of Indole Acetic Acid-Producing Endophytic Bacteria from Daun Dewa Plant (Gynura divaricata (L.) DC) Ratna Dewi Setyowati; Artini Pangastuti; Ari Susilowati
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23003

Indole acetic acid (IAA) is an auxin hormone that can regulate plant growth and development. Bacteria produce IAA through L-tryptophan metabolism. The purpose of this study was to determine the ability of endophytic bacteria isolated from Daun Dewa plants to produce IAA to increase the germination and growth of tomato seedlings. Isolation of endophytic bacteria was carried out by sterilizing plant surfaces. The production of IAA is done by adding L-tryptophan precursors. The results showed that there were six endophytic bacteria, and three isolates of them have the potential to produce IAA, namely: AD1.1, UD1.1, and UD2.1. The highest IAA concentration was produced by isolate AD1.1. Growth in sprouts is significantly influenced by the concentration of IAA; the higher the concentration of IAA, the longer the shoots and the roots. Based on the 16S rRNA gene sequence, these isolates belong to the genus Klebsiella.

Diversity, Vegetation Structure, Estimated Biomass and Carbon Stock in The Site of Limboto Lake of Gorontalo Regency Sukirman Rahim; Dewi Wahyuni K Baderan; Bella Saskia Arfa; Melisnawati H Angio; Muhammad Rifqi Hariri; Dwinda Mariska Putri
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23010

The development of Limboto Lake Site as the pilot area of Gorontalo Geopark is one step in rescuing the lake. This research aims to analyzed the vegetation structure, estimate biomass, and assess the carbon stock value of the vegetatio at the Limboto Lake site. This study employed a purposive sampling method. Important Value Index, a measure used to evaluate plant vegetation structure, was calculated based on Realtive Density, Relative Dominance, and Relative Frequency values. Biomass estimation and carbon stock assessment were conducted using allometric equations. The study’s finding showed that the vegetation surrounding Soekarno's landing area exhibited the highest IVI (sawlogs) scores for Swietenia mahagoni tree at 82.86%, Leucaena leucocephala saplings at 79.56%, and Manihot esculenta seedlings at 46.33%. Leucaena leucocephala consistently achieved the highest IVI score within the Limboto Lake area with a score as high as 76.90%, followed by Leucaena leucocephala on the sapling level with a score as high as 70.03%, and Nauclea orientalis on the seedling level with a score as high as 53.06%. Swietenia mahagoni, Samanea saman, and Leucaena leucocephala are three species with stems that predominate at the sawlog level and sapling, and these stems have high carbon absorption values.

A Diversity of Copepods in Gajah Mungkur in Wonogiri Regency, Central Java, Indonesia Efri Roziaty; Afrika Arianto; Vina Listiawati
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23143

GajahMungkur Reservoir is a freshwater ecosystem thatis located6kmsouthofWonogiri Regency,CentralJavaProvince. Zooplankton is the primary consumer in the food chain of aquatic ecosystems. One of the common zooplankton found in freshwater ecosystem is copepod. Copepod is sensitive organism and play an important role as bioindicatator of water quality. The aimed of this study was to investigate the copepods diversity in Gajah Mungkur Reservoir. The method applied to this study is exploration and application of purposive sampling techniques in sampling. Copepods were collected from three different station, namely Station 1 (fishing area), Station 2 (tourism area), and Station 3 (fish cage area or keramba). The abiotic parameters were found to be varied on each station. Results showed that Gajah Mungkur Reservoir has moderately diverse copepods species with the average of diversity index 1.21. There were 7 species of copepods found in Gajah Mungkur Reservoir which mostly dominated by Mesocyclops sp., especially in Station 1 (fishing area). Meanwhile Microcyclops sp. was only found in Station 3 (fish cage area or keramba), suggesting that this species has a potential as bioindicator in Gajah Mungkur Reservoir. This study suggest that changes in physicochemical parameters might influence the diversity and abundance of copepod in Gajah Mungkur Reservoir.

The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro Kishy Dhea Herlanda; Endang Nurcahyani; Mahfut Mahfut; Tundjung Tripeni Handayani
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.22948

Red spinach (Amaranthus tricolor L.) is a commercially valuable vegetable known for its high nutritional content and immune-boosting properties, making it popular among the Indonesian population. However, red spinach cultivation in Indonesia is still relatively low and inconsistent, necessitating efforts to enhance its cultivation. One potential approach is through in vitro culture techniques. This study aimed to investigate the impact of applying mung bean sprout [Vigna radiata (L.) R. Wilczek] extract on Murashige and Skoog medium, focusing on identifying the most effective concentration for in vitro growth of red spinach explants. The experimental design employed a Completely Randomized Design (CRD) with five concentration levels: 0%, 5%, 10%, 15%, and 20%. Each concentration was replicated five times, with each replication consisting of five red spinach seeds in individual culture bottles. Data analysis involved Levene's test, one-way ANOVA, and Tukey's Honestly Significant Difference (HSD) test at a significance level of 5%. The findings revealed that the application of mung bean sprout extract significantly influenced the height of plantlets, leaf count, root length, fresh weight, and soluble carbohydrate content of red spinach. The most effective concentration for promoting red spinach explant growth was determined to be 5%.

Implementation of Bacteria Isolate as a Fertilizer and Bioinsecticide Agent for the Mealybug Slamet Santosa; Umi Fatmawati
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.20516

This research about the implementation of bacteria in papaya plantation soil in Kebumen that has been isolated and characterized and purified. This study aims to determine the potential of local isolate bacteria from papaya agricultural soil: 1). as PGPR bacteria or fertilizing bacteria for chili, eggplant and tomato plants and 2) as a natural bioinsecticide. mealybugs on papaya plants. The samples used were bacteria from the preliminary isolation that had been carried out, namely from the genera Bacillus, Pseudomonas, Erwinia, Exyguobacterium, Serratia, Stenotropomonas, and Acinetobacter. The study was conducted using 50 stems of each plant. Bioinsecticides are applied to papaya trees that are attacked by mealybugs. Measurement of plant growth was measured at 7 days and 30 days after planting. As for bioinsecticides, spraying was carried out 3 times. The results of the study can be concluded in the form of the implementation of bacteria as PGPR which can increase the growth of cayenne pepper (Capsicum frutescens), purple eggplant (Solanum melongena L) and tomato (Solanum lycopersicum Mill) and can be used as a bioinsecticide to reduce mealybugs (Paracoccus marginatus) on papaya plants (Carica papaya L.)

Dragonfly Inventory and Activity in Kasang Kulim Zoo Area, Riau Miranda Delithalia; Radith Mahatma
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23006

Dragonflies have an important role in ecosystem balance as biological control agents and environmental bioindicators because they are sensitive to changes in water quality. Community activities at the Kasang Kulim Zoo can cause changes to water quality. When habitat conditions change, Odonata (dragonfly) will also changes, both in distribution and diversity. This study aims to identify, invent and determine the activity time of dragonflies found in the Kasang Kulim Zoo area. Research was conducted at the end of March to mid-April 2023. Sampling used exploration method by using insect nets. The results obtained a total of 152 individuals belonging to 14 species, four families and two suborders (Anisoptera and Zygoptera). The family Libellulidae totaled eight species, Gomphidae one species, Coenagrionidae four species and Platycnemididae one species. The Libellulidae family are active from 08.00 am to 05.00 pm, while dragonfly species from the Gomphidae family and the Zygoptera suborder are active from 08.00 am to 03.00 pm. Dragonfly activity is influenced by air temperature, air humidity and sunlight intensity.

Identification of Anti-Cancer Compounds in Medicinal Plants Using Metabolomic Approaches: A Review Yani Magfiroh
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23017

The modern lifestyle despite its comfort has made cancer a second leading public health problem, following cardiovascular disease. As chemotherapeutic has many side effects including resistance, recent attention has focused on plants which may provide a good opportunity for complementary cancer treatment. Several studies have begun to focus on metabolomic research in order to discover the mechanisms of action of diverse medicinal plants, investigate biomarkers, and comprehend cancer progression at metabolic rates. This study aims to analyze and highlight related metabolomic investigations in medicinal plants that demonstrate the potential of biomarker compounds and their processes in cancer disease. The research methodology uses a literature review that is compiled from many connected journals. According to the findings, bioactive substances present in medicinal plants can be used as biomarkers to disrupt the metabolism of enzymes, transporters, and signaling pathways in cancer cells. Utilized analytical platforms include HPLC, GC-MS, FTIR and NMR. Statistical analysis employs the Anova, PCA, and OPLS-DA methods. Finally, we reviewed biomarkers of medicinal plants and metabolomic pathways, with the result that anticancer compounds may exert their activity by various methods affecting DNA replication, cell cycle, migration and apoptosis.

Expression and Activation of Toll-Like Receptor 9 in Dengue Virus Infection: A Scoping Review Safari Wahyu Jatmiko; Riandini Aisyah
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23052

Toll-like receptors (TLR) are essential to pathogen recognition to activate innate immunity. TLR2, 3, 4, 6, 7, 8, and 9 are involved in immunity to dengue virus. The involvement of TLR9, known to recognize CpG DNA, in dengue virus (DVI) infection, an RNA virus, is unusual. We hypothesize that there is an elevated expression and activation of TLR9 through specific mechanisms in DVI. A scoping review was carried out to collect articles that have been written regarding this topic. Article searches were carried out on the PubMed (Medline), Proquest, EBSCO, and Google Scholar databases with inclusion criteria: articles in the form of research results using human subjects or human cells, conducted from 2013 to 2023, in English, and can be accessed in full -text. Gray articles, books, and review articles were excluded from the study. The literature search results found 821 articles, with eight articles meeting the criteria. Based on mapping, synthesis, and analysis of the articles obtained, it was found that there was increased expression and activation of TLR9 in the DVI due to the release of mtDNA.

The Growth of Red Spinach (Amaranthus tricolor L.) After Application of Bean Sprouts Extract [Vigna radiata (L.) R. Wilczek] on Murashige and Skoog Medium In Vitro Herlanda, Kishy Dhea; Nurcahyani, Endang; Mahfut, Mahfut; Handayani, Tundjung Tripeni
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.22948

Red spinach (Amaranthus tricolor L.) is a commercially valuable vegetable known for its high nutritional content and immune-boosting properties, making it popular among the Indonesian population. However, red spinach cultivation in Indonesia is still relatively low and inconsistent, necessitating efforts to enhance its cultivation. One potential approach is through in vitro culture techniques. This study aimed to investigate the impact of applying mung bean sprout [Vigna radiata (L.) R. Wilczek] extract on Murashige and Skoog medium, focusing on identifying the most effective concentration for in vitro growth of red spinach explants. The experimental design employed a Completely Randomized Design (CRD) with five concentration levels: 0%, 5%, 10%, 15%, and 20%. Each concentration was replicated five times, with each replication consisting of five red spinach seeds in individual culture bottles. Data analysis involved Levene's test, one-way ANOVA, and Tukey's Honestly Significant Difference (HSD) test at a significance level of 5%. The findings revealed that the application of mung bean sprout extract significantly influenced the height of plantlets, leaf count, root length, fresh weight, and soluble carbohydrate content of red spinach. The most effective concentration for promoting red spinach explant growth was determined to be 5%.

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Journal Mail Official
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Kota surakarta,

Jawa tengah

INDONESIA