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All Journal Biosfer: Jurnal Tadris Biologi Bioeksperimen: Jurnal Penelitian Biologi Jurnal Penelitian Pertanian Terapan Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat AGROSCRIPT Journal of Applied Agricultural Sciences Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Agroscript: Journal of Applied Agricultural Sciences Analit: Analytical And Environmental Chemistry
Tundjung Tripeni Handayani, Tundjung Tripeni
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Veterinary Other

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EFFECT OF CITRICACID ON THE SEEDLING GROWTH OF UPLAND RICE (ORYZA SATIVA L.) VARIETAS SITU BAGENDIT UNDER ALUMINUM STRESS Dwiningrum, Riza; Zulkifli, Zulkifli; Handayani, Tundjung Tripeni
Jurnal Penelitian Pertanian Terapan Vol 16, No 3 (2016)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v16i3.48

Abstract

The objective of this research was to know whether soaking of upland rice seed in citric acid solution  could  alleviate  subsequent  growth  of  seedling  under  aluminum  stress.  The experiment was conducted in plant physiologylaboratory, Department of Biology, Faculty of Mathematic and Natural Science, Lampung University on January 2016, and implemented in2 x 3 factorial designs. Factor A was aluminum with two levels: 0 mM, 5 mM and factor B was Citric Acid with three levels: 0Mm, 5Mm, 10Mm. Variables in this research were shoot length, seedling fresh weight, relative water content, and shoot root ratio. Analysis of variance was conducted at 5% significant level. If   interaction factor A and B was not significant, main effect was determine with lsd test at 5% significant level, but if  significant simple effect was determine with F test at 5% significant level. The result showed that interaction beetwen aluminum and citric acid decreased the shoot length 16-31%. Aluminum decreased fresh weight of seedling 11%, relative water content 3%, and shoot root ratio decreased 15%. It was concluded that soaking upland rice seed in citric acid solution was not able to alleviate subsequent growth of seedling under aluminum stress. Keyword: citric acid, aluminum, upland rice, shoot length, seedling fresh weight
PENGARUH ASAM SITRAT, ALUMINIUM, DAN INTERAKSINYA TERHADAP PERTUMBUHAN KECAMBAH JAGUNG HIBRIDA (ZEA MAYS L.) VARIETAS BISI- 18 Safutri, Wina; Zulkifli, Zulkifli; Handayani, Tundjung Tripeni
Jurnal Penelitian Pertanian Terapan Vol 16, No 3 (2016)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v16i3.92

Abstract

The objective of this research was to know effect of citric acid, aluminum, and their itsinteraction on the seedling growth of hibrida maize (Zea mays L.) Var. Bisi-18. Theexperiment was conducted during January 2016 in Plant Physiology Laboratory,Department of Biology, Faculty of Mathematics and Natural Science, University ofLampung. The experiment was implemented in 2x3 factorial experiments. Factor A wasAluminum with 2 levels: 0 mM, 5 mM. Factor B was Citric Acid with 3 levels: 0 mM, 5 mM,and 10 mM. After selected corn seeds were soaked in citric acid solution for 24 hours, andgerminated in plastic tray. After 7 days seedlings were planted in plastic containers thatcontain aluminum solution. Variables in this research were shoot length, seedling freshweight, shoot root ratio, and relative water content after 7 days of growing period. Analysisof variance was conducted at 5% significant level. If interactions between aluminum andcitric acid was not significant, main effect was determined at 5% significant level with LSDtest. If interactions between aluminum and citric acid was significant, simple effect wasdetermined at 5% significant level with F test. The result showed that no aluminum effectobserved on all variables, and citric acid affected negativly all variables. No interctionsbetween aluminum and citric acid on all seedling growth variables of maize hibrida Var.Bisi-18. It is concluded aluminum 5mM was not able to cause stress in maize seedling. Inno stress condition citric acid has negative effect on maize seedling growth.Keyword: Citric acid, Aluminium, Maize Seedling, Shoot Length, Seedling Fresh Weight.
Identification of dendrobium Natural Orchids in Liwa Botanical Garden based on leaf morphological characters Mahfut, Mahfut; Handayani, Tundjung Tripeni; Wahyuningsih, Sri; Sukimin, Sukimin
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol 5, No 1 (2021)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v5i1.2938

Abstract

Lampung Indonesia is one of the provinces on the island of Sumatra, which has a flora conservation area located in Liwa Botanical Garden, West Lampung Regency. One of the flora species in the area with a high level of diversity is the orchid plant. The Dendrobium is the largest orchid species, where the public widely hunts this orchid for an economic reason due to the beauty of its various flower shapes and colors. Given the high level of human encroachment, this can threaten the existence of natural orchid plants, causing loss of their original habitat and causing the extinction of orchid species. Based on data in Liwa Botanical Garden, there has been no further identification of natural orchids as a plant conservation effort. To protect and immediately save these natural orchids, it is necessary to identify the Dendrobium orchid species based on the leaf morphological approach. This research will be conducted in April-September 2020. Observations of leaf morphology include leaf shape, leaf tip shape, leaf base shape, leaf edge, leaf reinforcement, leaf arrangement, leaf surface texture, leaf symmetry, and leaf sitting based on guidelines for ornamental plant characterization books. Observations as secondary data were added to the character of the pseudobulb shape and orchid habitat. Observations as secondary data were added on the habitat character of pseudobulbs and orchids. The implementation of this activity is still in the period of the Covid-19 pandemic, so most of the activities are carried out online. The coaching and training phase was conducted through internal virtual discussions on Tuesday, July 7, 2020, while the coaching phase was conducted through meetings or verbal communication via telephone groups and WhatsApp. Based on the evaluation data, it is known that there is an increase in knowledge and understanding of training participants by 21.64 points. The percentage increase in value before and after training is 34.72%. Orchid-related activities at Liwa Botanical Garden were never carried out, thus imparting knowledge to Liwa Botanical Garden technicians and workers on the development of natural orchid identification methods based on the morphological character in local natural resource conservation in the 4.0 industrial revolution era.
Efek Pemberian Ekstrak Umbi Kembang Sungsang (Gloriosa superba L.) Pada Pembentukan Planlet Pisang (Musa paradisiaca L.) Kultivar Kepok Kunig Secara In Vitro Wahyu, Julaiha; Ernawiati, Eti; Handayani, Tundjung Tripeni; Wahyuningsih, Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4377

Abstract

The world community, including Indonesia much like bananas because it has a relatively low price and good nutritional content. Banana kepok is a type of banana favored by the public because it tastes good and sweet. However, the obstacle that is often faced in kepok bananas is the difficulty in providing quality banana seeds quickly. Therefore, alternative technology is needed through tissue culture techniques. The purpose of this study was to determine the effect of adding breech flower tuber extract in culture media on the formation of banana plantlets of yellow kepok cultivar and to obtain scientific information about the growth profile of yellow kepok plantlets due to the addition of breech flower tuber extract to culture media. This research was conducted at the MTC Laboratory, PT. Great Giant Pineapple PG 4 Labuhan Ratu, East Lampung from December 2019 - April 2020 using a Completely Randomized Design (CRD) with ten replications consisting of the addition of 10% breech flower tuber extract as the primary treatment, 0.1% pure colchicine as a positive control, and without addition as a negative control. The results showed that the addition of 10% breech flower tuber extract in the culture media caused the growth of yellow kepok banana plantlets to be slower than the addition of 0.1% colchicine. Then in the media, with the addition of 10% breech flower tuber extract, the number of shoots, shoot height, number of better root and leaf area than the media without expansion, but 0.1% colchicine still showed the best results.
Pengaruh Konsentrasi Giberelin dan Lama Perendaman terhadap Viabilitas Benih Kopi (Coffea canephora Pierre ex A. Froehner) Armalia, Inayah; Wahyuningsih, Sri; Handayani, Tundjung Tripeni; Mahfut, Mahfut
AGROSCRIPT: Journal of Applied Agricultural Sciences Vol 6 No 1 (2024): June
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/agroscript.v6i1.1554

Abstract

Benih kopi robusta sulit berkecambah karena memiliki kulit biji yang keras sehingga menghambat proses penyerapan air dan gas. Upaya yang dapat dilakukan untuk mempercepat pertumbuhan kecambah yaitu dengan merendam benih kopi robusta dalam larutan giberelin yang merupakan zat pengatur tumbuh. Penelitian ini bertujuan untuk mengetahui konsentrasi giberelin, lama perendaman, dan kombinasi kedua perlakuan yang efektif dalam mempercepat pertumbuhan kecambah benih kopi robusta. Penelitian dilakukan di Laboratorium Botani, FMIPA Universitas Lampung dari Bulan Oktober sampai Desember 2022. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) faktorial. Faktor pertama yaitu konsentrasi giberelin yang terdiri dari 0 ppm (G0), 85 ppm (G1), dan 170 ppm (G2). Faktor kedua 2 yaitu lama perendaman yang terdiri dari 1,5 jam (T1) dan 3 jam (T2). Benih kopi robusta yang telah diberi perlakuan disemai dan diamati selama 50 hari. Parameter pengamatan meliputi, persentase pertumbuhan kecambah, laju pertumbuhan kecambah, panjang akar terpanjang kecambah, dan panjang hipokotil. Data hasil penelitian dianalisis dengan ANOVA dan uji Beda Nyata Jujur (BNJ) pada taraf 5%. Hasil penelitian menunjukkan bahwa kombinasi perlakuan konsentrasi 85 ppm (G1) dan lama perendaman 1,5 jam (T1) efektif dalam mempercepat pertumbuhan kecambah benih kopi robusta dan memberikan pengaruh yang paling positif pada parameter persentase pertumbuhan kecambah, laju pertumbuhan kecambah, panjang akar terpanjang kecambah, dan panjang hipokotil.
Effectiveness of Coconut Water and Atonics on the Growth of Eggplant (Solanum melongena) Seedlings of the Laguna F1 Variety: EFEKTIVITAS PEMBERIAN AIR KELAPA DAN ATONIK TERHADAP PERTUMBUHAN KECAMBAH TERONG UNGU (Solanum melongena) VARIETAS LAGUNA F1 Wulandari, Adelia saviera; Handayani, Tundjung Tripeni; Handayani, Kusuma; Wahyuningsih, Sri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.253

Abstract

Eggplant (Solanum melongena L.) is an authentic plant native to tropical climates, widely favored by the community, and available at an affordable price. The demand for eggplant in Indonesia reached 69,963 tons in 2015 and has continued to increase to 74,081 tons. Based on FAO data in 2016, Indonesia is one of the eggplant-producing countries, ranking sixth in the world after China, India, Egypt, and Turkey. The Laguna F1 eggplant variety is known for its quality and resistance to bacteria-causing wilting. To minimize the cultivation time of eggplants, it is necessary to use readily available natural fertilizers, one of which is coconut water. The utilization of coconut water as fertilizer is due to its content of various hormones that play a crucial role in plant growth, namely auxin, cytokinin, and gibberellin. The purpose of this research is to investigate the effects of coconut water and atonic, determine the optimum concentrations, and understand the interaction between coconut water and atonic in the growth of eggplant plants. The research was conducted in the Botany Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Lampung, in October-November 2019, using a 2x3 factorial design. The first factor involved coconut water at concentration levels of 25%, and 50%, and compared with a control group (0%). The second factor was the atonic solution with a concentration of 10 ml/l, compared with a control. The treatments were repeated in five replications, resulting in 30 experimental units. Observed variables included plant height, fresh weight, dry weight, relative water content, and chlorophyll content. The results of this research can be summarized as follows: the application of coconut water and atonic has a positive effect on the growth of eggplant plants. Additionally, the combined induction of coconut water at a concentration level of 25% and atonic at 0% demonstrated superior outcomes in terms of plant height, fresh weight, dry weight, relative water content, and chlorophyll content in eggplant plants.
Isolation and Identification of Fungi from Bactrocera dorsalis as Candidate Entomopathogenic Fungi Damayanti, Rizka Dwi; Emantis Rosa; Setiawan, Wawan Abdullah; Handayani, Tundjung Tripeni
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.359

Abstract

Cultivation of horticultural crops especially fruits, has long been practiced in Indonesia. Citrus plants are one of the main fruit commodities developed in Indonesia. Increased citrus production is inseparable from challenges, especially in controlling harmful pest attacks. Bactrocera fruit fly has attracted worldwide attention because of its ability to attack more than a hundred horticultural crops and cause crop failure. Various ways have been done to control Bactrocera, such as protecting the fruit with wrapping and even the use of synthetic insecticides that cause many losses. To overcome this, innovations are needed using biological control. Entomopathogenic fungi are potential candidates for bioinsecticides. This study aims to isolate and identify entomopathogenic fungi from Bactrocera dorsalis from citrus plants. The entomopathogenic fungus isolation method uses the moist chamber method. Then the fungal isolates will be identified macroscopically and microscopically. The isolation results show that five types of fungi are identified from the body of Bactrocera dorsalis. The five fungi can be used as candidates for entomopathogenic fungi.
Colchicine Effect of Mitosis Root Tip by Banana Kepok Yellow (Musa paradisiaca L.) Polyploid Explants Yana, Dellya Vivi; Ernawiati, Eti; Agustrina, Rochmah; Handayani, Tundjung Tripeni
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.210

Abstract

Colchicine can inhibit spindle formation so that doubled chromosomes fail to separate during anaphase cytologically to induce mutations that produce polyploid plants. Yellow kepok banana is a triploid banana. The supply of yellow kepok bananas is hampered by conventional cultivation methods and the limited availability of quality seeds. This study aims to determine the effect of adding colchicine compound to tissue culture media on the mitotic index, chromosome number, and chromosomal abnormalities in yellow kepok bananas and the formation of polyploid plantlets. This study was included in the experimental study by conducting mitotic preparation and observing morphology after the test sample was induced by colchicine. The results of the research that has been done show that the addition of the compound colchicine to the tissue culture medium of yellow kepok banana causes a decrease in the mitotic index, an increase in the number of chromosomes, the presence of chromosomal abnormalities, the formation of polyploid plantlets, and an increase in the number of roots, but there is a reduction in root length.
Quantitative and Descriptive Paradermal Anatomy of Dendrobium discolour and Phalaenopsis amabilis Orchid Leaves Handayani, Tundjung Tripeni; Pramono, Eko
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.216

Abstract

Dendrobium dan Phalaenopsis merupakan sumber daya genetik anggrek  yang semakin langka, sehingga perlu dilakukan upaya pemuliaan untuk melestarikannya. Tujuan penelitian ini untuk mengetahui karakter morfologi dan anatomi Dendrobium dan Phalaenopsis. Metode yang digunakan yakni sayatan paradermal pada epidermis daun anggrek yang diamati secara mikroskopik, kuantitas anatominya, dan kualitas gambarnya. Penelitian ini menggunakan faktor tunggal dengan dua jenis anggrek yang digunakan dalam rancangan acak kelompok, serta variabel kualitatif dan kuantitatif. Hasil penelitian menunjukkan bahwa kedua permukaan Dendrobium memiliki Panjang dan lebar stomata yang hampir sama, sedangkan pada Phalaenopsis pada permukaan bawah daun memiliki ukuran stomata yang sedikit lebih besar dibandingkan dengan permukaan atas. Dendrobium memiliki bentuk sel epidermis atas heksagonal, jenis stomata parasite dan bagian bawah tetrasitik, sedangkan daun Phalaenopsis di permukaan bawah dan atas adalah tetrasitik. Stomata terdapat pada permukaan atas dan bawah daun anggrek Dendrobium dan Phalaenopsis. Berdasarkan dari penelitian yang telah dilakukan terdapat perbedaan pada jumlah stomata, bentuk sel epidermis dan jenis stomata pada permukaan daun atas dan bawah anggrek Dendrobium maupun Phalaenopsis.   Kata kunci: Anatomi, Anggrek, Dendrobium, Paradermal, Phalaenopsis.   Dendrobium and Phalaenopsis are genetic resources of orchids that are increasingly rare, so breeding efforts are needed to preserve them. The purpose of this study was to determine the morphological and anatomical characters of Dendrobium and Phalaenopsis. This research was conducted in April-September 2021 at the Botanical Laboratory of the Biology Department, University of Lampung. The method used is a paradermal incision on the epidermis of orchid leaves which was observed microscopically, the anatomical quantity, and the image quality. This study used a single factor with two types of orchids used in a randomized block design, as well as qualitative and quantitative variables. The results showed that the two surfaces of Dendrobium had almost the same length and width of stomata, while Phalaenopsis on the lower surface of the leaves had stomata size which was slightly larger than the upper surface. Dendrobium has a hexagonal upper epidermal cell shape, parasite stomata type and the bottom is tetracytic, while Phalaenopsis leaves on the lower and upper surfaces are tetracytic. Stomata are found on the upper and lower surfaces of the leaves of Dendrobium and Phalaenopsis orchids. Based on the research that has been done, there are differences in the number of stomata, the shape of the epidermal cells and the type of stomata on the upper and lower leaf surfaces of Dendrobium and Phalaenopsis orchids.   Keywords: Anatomy, Orchid, Dendrobium, Paradermal, Phalaenopsis.
Efek Alelopati Ekstrak Daun Babandotan (Ageratum conyzoides L.) terhadap Perkecambahan dan Pertumbuhan Kecambah Padi Gogo Varietas Inpago 8 Diana, Elsi; Zulkifli, Zulkifli; Handayani, Tundjung Tripeni; Lande, Martha Lulus
Biosfer: Jurnal Tadris Biologi Vol 10 No 1 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v10i1.4197

Abstract

Tujuan dari penelitian ini adalah membuktikan bahwa pada fase awal pertumbuhan vegetatif padi gogo varietas Inpago 8 tahan terhadap alelopati dari ekstrak daun kering babandotan (Ageratum conyzoides L.). Penelitian ini dilakukan pada bulan November – Desember 2018 di Laboratorium Botani Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Lampung. Penelitian ini menggunakan metode RAL (Rancangan Acak Lengkap) dengan ekstrak daun kering babandotan sebagai faktor utamanyayang terdiri atas 5 taraf konsentrasi yaitu 0% b/v, 2,5% b/v, 5% b/v, 7,5% b/v, dan 10% b/v dan terdiri dari 5 kali ulangan. Parameter yang diukur dalam penelitian ini adalah daya kecambah, panjang tunas, berat segar, berat kering,kadar air relatif, dan kandungan klorofil a,b serta total kecambah. Homogenitas ragam ditentukan dengan uji Levene pada taraf nyata 5%. Analisis ragam dan uji BNJ dilakukan pada taraf nyata 5 %. Hasil penelitian ini menunjukkan bahwa ekstrak daun kering babandotan tidak bersifat alelopati terhadap perkecambahan benih padi gogo varietas Inpago 8. Pada konsentrasi 7,5% b/v menstimulasi pertumbuhan kecambah padi gogo Inpago 8 yang ditunjukkan oleh peningkatan panjang tunas dan berat segar tunas.
Co-Authors Armalia, Inayah Bambang Irawan Damayanti, Rizka Dwi Diana, Elsi Eko Pramono Emantis Rosa Endang Nurcahyani Ernawiati, Eti Fachry Abda El Rahman Hadi, Muhammad Syamsoel Herlanda, Kishy Dhea Krisna Rendi Awalludin Kusuma Handayani, Kusuma Mahfut Martha Lulus Lande, Martha Lulus Mochammad Imron Awalludin Oktaviani, Ratna Riza Dwiningrum Rochmah Agustrina Sani, Dian Putri Sri Wahyuningsih Sukimin Tumiar Katarina Manik Wahyu, Julaiha WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH Wina Safutri Wulandari, Adelia saviera Yana, Dellya Vivi Zulkifli Zulkifli