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BIO-SITE |BIOLOGI Sains Terapan

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Published by Universitas Jambi

ISSN : - EISSN : 25026178 DOI : -

Core Subject : Education, Agriculture,

Agriculture, Biological Sciences & Forestry Education

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Articles 6 Documents

Search results for , issue "Vol. 4 No. 1 (2018): Bio-Site" : 6 Documents clear

Pengaruh Pemberian Bokashi Campuran Alang-alang (Imperata cylindrica L.) dan Kotoran Kambing Terhadap Pertumbuhan Tanaman Sawi (Brassica rapa L.) Susilo Susilo; Liza Verizza Triansyah; Maryanti Setyaningsih
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing terhadap pertumbuhan tanaman sawi (Brassica rapa L.). Metode penelitian yang digunakan adalah metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL), yang terdiri dari 5 perlakuan dengan 5 ulangan. Parameter pertumbuhan yang diamati dalam penelitian ini meliputi berat segar, panjang akar dan berat kering tanaman. Hasil penelitian menunjukkan bahwa perlakuan P2 (dosis 20 g) mendapatkan nilai rata-rata terbaik untuk berat segar dan berat kering yaitu secara berturut-turut 30,692 g dan 1,680 g, dengan nilai persentase yaitu 301,49% dan 390,70% terhadap kontrol. Sedangkan pada parameter panjang akar tanaman hasil nilai rata-rata paling baik terdapat pada P3 yaitu 5,46 cm dengan persentase 177,27% terhadap kontrol. Berdasarkan uji statistik yang dihitung menggunakan uji ANAVA 1 Faktor, pertumbuhan tanaman sawi pada parameter berat segar, panjang akar dan berat kering tanaman yang secara berturut-turut menunjukkan bahwa Fhitung ≥ Ftabel, yaitu 3,40 > 2,87; 2,87 ≥ 2,87 dan 4,86 > 4,43. Oleh karena Fhitung ≥ Ftabel, maka kelima perlakuan dinyatakan berpengaruh signifikan terhadap parameter berat segar dan panjang akar tanaman dan berpengaruh sangat signifikan pada parameter berat kering tanaman. Berdasarkan hasil tersebut dapat disimpulkan bahwa bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing berpengaruh terhadap pertumbuhan tanaman sawi (Brassica rapa L.).

Deteksi Cemaran Bakteri Escherichia coli dalam Jaruk Tigaron pada Pasar Sungai Andai dan Pasar Lama Kota Banjarmasin Ika Oksi Susilawati; Rini Saidah
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Jaruk tigaron is one of the traditional fermented foods originating from South Kalimantan made by fermented tigaron flowers (Crataeva nurvala, Buch HAM). The process of making traditionally allows contamination by the bacteria Eschericia coli (E.coli). Escherichia coli is the most commonly used a microbe as an indicator of faecal contamination in water, even food or beverages, including various types of jaruk. This final project aims to detect Escherichia coli on jaruk tigaron thatdistributed in Banjarmasin. The method was used in detecting Escherichia coli is the Most Probable Number (MPN) with five test stages are estimation test, confirmatory test, complementary test, biochemical test (TSIA and IMVIC). The media was used is a Lactose Broth Single Strength (LBSS) by 3 series for forecasting test, Brilliant Green Bile Broth (BGLB) media for confirmatory test, Eosin Methylene Blue Agar (EMBA) media for complementary test, TSIA test and IMVIC test. The test results was obtained by MPN index on samples (a) 28 samples (b)> 1.100 and sample (c) 20. The detection results is Escherichia coli on jaruk tigaron thatdistributed in Banjarmasin declared negatively containing Escherichia coli but only contain Coliform bacteria. Based on SNI 7388: 2009, the determination of the maximum limit of microbial contamination in food is the absence of Coliform and Escherichia coli bacteria in 100 ml of sample

POTENSI Jatropha curcas L. SEBAGAI ANTISEPTIK PADA PERTUMBUHAN Staphylococcus aureus, Escherichia coli, dan Candida sp. Erna Harfiani; Aulia Chaerani
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Skin disease is still a health problem in the world, especially tropical countries, including Indonesia, it needs normal control of flora among others with antiseptic. Jatropha curcas L., which contains secondary metabolite compounds suspected as an antiseptic on the skin. The study aimed to evaluate Jatropha sap can inhibit the growth of Staphylococcus aureus, Escherichia coli, and Candida albicans, and to know the optimal concentration of Jatropha curcas by invitro. The antiseptic activity of Jatropha sap was tested using agar diffusion method (Kirby-Bauer) with Mueller Hinton Agar medium and Saboround Dextrose Agar at concentrations 20%, 40%, 60%, 80% and 100. The results showed that Jatropha sap can inhibit the growth of Staphylococcus aureus, and Escherichia coli but not in Candida albicans. Treatment of 100% Jatropha sap concentration is the most optimal concentration in inhibiting the growth of Staphylococcus aureus, and Escherichia coli with an inhibitory zone of 9.75 ± 0.27 mm (medium antiseptic power) and 10.61 ± 0.2 mm (strong antiseptic power). The results show that the sap of Jatropha curcas L. grown in Indonesia has a good potential to be used as an antiseptic made from nature. Penyakit pada kulit masih merupakan masalah kesehatan di dunia terutama negara beriklim tropis, termasuk Indonesia, sehingga diperlukan pengendalian flora normal diantaranya dengan antiseptik. Getah jarak pagar (Jatropha curcas L.) yang mengandung senyawa metabolit sekunder diduga berfungsi sebagai antiseptik pada kulit. Penelitian bertujuan mengevaluasi apakah getah jarak pagar dapat menghambat pertumbuhan Staphylococcus aureus, Escherichia coli dan Candida albicans, dan mengetahui konsentrasi optimal getah jarak pagar secara invitro. Aktivitas antiseptik getah jarak pagar diuji dengan menggunakan metode difusi agar (Kirby-Bauer) dengan medium Mueller Hinton Agar (MHA) dan Saboround Dextrose Agar (SDA) pada konsentrasi 20%, 40%, 60%, 80% dan 100. Hasil penelitian menunjukkan getah jarak pagar dapat menghambat pertumbuhan bakteri Staphylococcus aureus, dan Escherichia coli namun tidak pada jamur Candida albicans. Perlakuan getah jarak pagar konsentrasi 100% merupakan konsentrasi paling optimal dalam menghambat pertumbuhan Staphylococcus aureus, dan Escherichia coli dengan zona hambat 9,75 ± 0,27 mm (daya antiseptik sedang) dan 10,61 0,2 mm (daya antiseptik kuat). Hasil menunjukkan getah jarak pagar yang banyak tumbuh di Indonesia mempunyai potensi yang cukup baik untuk dipergunakan sebagai antiseptik berbahan alam. Keyword: Antiseptik, Escherichia coli, Candida albicans, Jarak pagar (Jatropha curcas L.), Staphylococcus aureus

Kemampuan Air Kelapa Muda Sebagai Antimikroba Terhadap Bakteri Escherichia coli Penyebab Diare Arif Mulyanto; Ikhsan Mujahid; Tyas Ulfah Khasanah
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Diarrhea is a circumstance when the body loses much fluid and electrolyte through feces. According to the data obtained by Ministry of Health of the Republic of Indonesia (2016), in 2015 there were 18 cases of healthemergency of diarrhea, spread over 11 provinces and 18 regencies/cities. There were 1.213 people who got the disease, 30 of them died. Partially, villagers treated diarrhea by drinking young coconut water. This research aimsto discover the effectiveness of several kinds of young coconut water as antimicrobials to bacteria that can causediarrhea (bacteria test) such as Escherichia coli. In this research the inhibitionof young coconut water sample toward above-mentioned bacteria was held. Besides, a phytochemical screeningtest was also conducted to find out the antimicrobial substances contained in young coconut water. The data of young coconut water antimicrobial testing toward Escherichia coli, wereanalyzed by employing analysis of varian (ANOVA) with F test on 95% confidence level. Data testing wasfollowed by Duncan test on 95% confidence level. Research results indicated that young coconut water had antimicrobial ability toward Escherichia coli. Based on the result ofphytochemical screening of the young coconut water (all treatments) it was only secondary metabolite tannins detected. All treatments (of the young coconut water) could inhibit tested bacteria. It is allegedly because thewater contained tannins. The water of green coconut (used as a traditional medicine) was an effective inhibitor of bacteria growth bacteri. On E. coli the inhibition zone diameter was 11,6 mm. Keywords: young coconut water, antimicrobial, bacteria, diarrhea

ISOLASI DAN IDENTIFIKASI Shigella sp. PENYEBAB DIARE PADA BALITA Fitratul Aini
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Diarrhea is the highest cause of death for toddlers. This study aims to isolate and identify the bacteria Shigella sp. causes of diarrhea in toddlers. Diarrhea samples were obtained from RSIA Annisa, Jambi City then isolation was carried out at the Biotechnology and Engineering Laboratory of the Faculty of Science and Technology, Jambi University using SSA media and dilution and biochemical tests were carried out. The results of bacterial isolates were characterized and obtained by Salmonella sp. isolates. With the characteristic of black colonies, capable produce H2S gas and can ferment sugar.

Keanekaragaman Kumbang Sungut Panjang (Cerambycidae) di Kawasan Hutan Pendidikan Universitas Jambi Tia Wulandari; Winda Dwi Kartika
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Abstrak Serangga adalah kelompok hewan yang memiliki keragaman lebih tinggi daripada yang lain. Serangga memiliki manfaat dalam ekosistem dan juga dapat membantu aktivitas manusia. Cerambycid adalah sejenis serangga yang dapat mengubah komponen organik pada kayu dan batang mati. Tujuan dari penelitian ini adalah untuk mengetahui keragaman Cerambycid di hutan pendidikan Universitas Jambi. Penelitian ini diadakan pada bulan Agustus sampai November 2016. Sampel dikumpulkan dengan menggunakan perangkap Arthocarpus di 3 tempat yang berbeda. Jumlah keramik yang telah dikumpulkan adalah 561 individu dan termasuk 14 spesies. Hasil analisis data menunjukkan tingkat keragaman 0,631. Nilai indeks keanekaragaman dapat menunjukkan kondisi hutan pendidikan Universitas Jambi yang baik sebagai habitat kumbang Cerambycid. Kata Kunci: kumbang, Cerambycid, keragaman, kumbang sungut panjang Abstract Insects are group of animals that have higher diversity than anothers. Insects have benefits in ecosystem and also can help human activities. Cerambycid is a kind of insects that can transform organic matters on wood and dead stem. The aim of this study is to know the diversity of Cerambcyid in Jambi University educational forest. It is held on August untill November 2016. Samples was collected by using Arthocarpus trap in 3 different spot. The number of Cerambycid that had collected are 561 individuals and belong to 14 species. The result of data analysis showed that Cerambcyid diversity rate is classified into medium rate with diversity value reaches 0,631. Diversity index value can show Jambi University educational forest condition which is good as the Cerambcyid beetle. Keyword: Beetle, Cerambycid, Diversity, Long Horn Beetle

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