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Y. Andi Trisyono
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 11, No 1 (2005)" : 7 Documents clear
Interaksi Geminivirus pada Kejadian Inokulasi Ganda Sri Hendrastuti Hidayat; Rina Rachmawati; Puji Lestari; Noor Aidawati
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12105

Abstract

Incidence of geminivirus infection has been caused severe losses on various economically important crops. This losses may cause by single infection or mix infection of several strain of geminiviruses. In this experiment we used two strain of geminiviruses, geminivirus infecting chillipepper from Segunung, West Java and geminivirus infecting tomato from Lembang, West Java (GVToL), to study the effect of geminivirus mix infection on the symptom expression and the capability of each strain to multiply in the host cell. Coefficient of dissimilarity between this two strain of geminiviruses is 7.5. When the two strain of geminiviruses was inoculated together to tomato plants by insect vector; Bemisia tabaci, the symptoms on infected plants is slightly different but less severe than those on plants inoculated with each strain separately. Detection with polymerase chain reaction (PCR) followed by restriction enzyme digest of PCR product revealed that GVToL multiply better in the infected host cell.
Pengaruh Berbagai Ekstrak Metanol Tumbuhan terhadap Mortalitas Juvenil Instar-2 dan Penetasan Telur Nematoda Sista Kentang (Globodera rostochiensis) Iis Nur Asyiah; Elin Yulinah; Mumu Sutisna; Buchari Buchari
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12113

Abstract

The research was conducted to know the influence of methanol extracts from 19 plant species to the second-stage juvenil (J2) mortality and to the hatching of the potato cyst nematode, Globodera rostochiensis, the new pest of potato in Indonesia. The research was conducted by using complete random design, where each treatment was repeated 3 times. Cyst hatching was conducted by using ZnSO4 10-2 M. Data obtained to be analyzed with the ANOVA and continued with the DMRT 5%. The result indicated that from 19 species of examined plant, clove flower and leaf methanol extract with concentration 10.000 ppm inflicted 100% and 96,7% J2 mortality and others inflicted <50% 12 mortality. Clove flower and leaf methanol extract permanently inhibited of cyst hatching, methanol extract of Andrograpis puniculata, Capsium frutescens, Chrysanthemum sp., and Allium sativum inhibited of cyst hatching non permanently, while other plants extract methanol didn't inhibit of cyst hatching.
Preferensi Parasitoid Trichogrammatoidea spp. yang Memarasit Telur Penggerek Buah Merah Jambu Pectinophora gossypiella Saunders (Lepidoptera; Gelechiidae) terhadap Beberapa Inang Dwi Adi Sunarto; Nurindah Nurindah; Sujak Sujak
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12114

Abstract

Pink bollwonn Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae), is one of the key pests of cotton. Exploration of its egg parasitoids in East Java, yields Trichogrammatoidea spp. The research objective are to study host acceptance of the parasitoids to several levels of host age and host species for a consideration of selection in using the parasitoids as candidates of biocontrol agent of pink bollworm. The study was carried out in Biocontrol Laboratory of Indonesian Tobacco and Fiber Crops Research Institute (IToFCRI) from April to December 2002 with laboratory condition (T: 25 - 27°C; RH: 65-70%). The treatments consist of combinations of the parasitoid origin (Trichogrammatoidea sp A and Trichogrammatoidea sp L); host species (eggs of Corcyra cephalonica, P. gossypiella, and Helicoverpa armigera) and host ages (1, 2 and 3 days). The number of replicates is 10. Preference level was assessed by using continuous observation method. Trichogrammatoidea sp A is most preferred to P. gossypiella eggs, significantly different with that of C. cephalonica and H. armigera eggs. Host preference of Trichogrammatoidea sp L to P. gossypiella and C. cephalonica eggs is not significantly different and higher than that of H. armigera eggs. Both parasitoid species have no different preference to P. gossypiella and H. armigera eggs, however, Trichogrammatoidea sp L has a higher preference to C. cephalonica eggs than Trichogrammatoidea sp A. Host preference of both species was not affected by the age of all three species of host eggs.
Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus Sholeh Avivi; Sudarsono Sudarsono; Satriyas Ilyas; Hajrial Aswidinnoor
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12103

Abstract

The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.
Variasi Kebugaran Jenis/Strain Trichogramma pada Telur Plutella xylostella (L.) (Lepidoptera: Plutellidae) Siti Herlinda
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12115

Abstract

Laboratory experiments were conducted to evaluate fitness traits of trichogrammatid species/strains reared on eggs of the diamondback moth, Plutella xylostella (L.). The experiments used eleven trichogrammatid species/strains collected from different host species and locations. They were Trichogrammatoidea cojuangcoi Nagaraja collected from parasitized P. xylostella eggs in Jarai, Muarasiban, Kerinjing, and Bogar, Trichogrammatoidea sp. A and B collected from parasitized Helicoverpa armigera (Hubner) eggs in Bogor, Trichogrammatoidea armigera Nagaraja collected from parasitized H. armigera eggs in Bogor, Trichogramma chilonis Ishii, Trichogramma japanicum Ashmead, Trichogramma australicum Girault, and Trichogrammatoidea nana (Zehntner) collected from parasitized Chilo sp. eggs in Cinta Manis, South Sumatera. When a female of Trichogramma was constantly exposed to 100 P. xylostella eggs per day throughout her lifetime, T. cojuangcoi from Jarai produced more parasitized host eggs (50.67%) and progenies (50.67 adults/female) compared to other species/strains. Sex ratio was predominantly female, the most female progenies were produced ( 38 adults/ female) by T. cojuangcoi from Jarai. There were no significant differences in larval (0-3.27%) and pupal (0-3.75%) mortality of all species/strains. Survival emerging adults of T. japanicum (90.24%) were significantly lower than those of other species/strains. These data showed that T. cojuangcoi from Jarai could be developed as biocontrol agents of P. xylostella.
Analisis Aktivitas Enzim Peroksidase dan Kandungan Asam Salisilat dalam Tanaman Cabai Merah yang Diinduksi Ketahanannya terhadap Cucumber Mosaic Virus (CMV) oleh Ekstrak Daun Bunga Pukul Empat (Mirabilis jalapa) Hersanti Hersanti
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12104

Abstract

Leaf crude extract of Mirabilis jalapa is an inducer agent of systemic resistance of red chilli to CMV. This experiment was objected to study the activity of peroxidase enzyme and the concentration of salicylic acid in the induced resistant plant to CMV by M. jalapa leaf crude extract. The results showed that the induced resistant plant to CMV by leaf crude extract of M. jalapa had a low CMV disease intensity, low content of virus, increased the activity of peroxidase enzyme 2 - 10 times; increased the salicylic acid 1.6 - 5 times. There were a significant interaction effect between disease intensity of CMV and salicylic acid content (r = 0.94), middle interaction effect between disease intensity of CMV and salicylic acid content (r = 0.46), low interaction effect between the virus content and disease intensity of CMV (r = 0.32), and salicylic acid and activity of peroxidase enzyme (r = 0.39); and no interaction effect between virus content and salicylic acid content (r = 0.05), and between virus content and activity of peroxidase enzyme ( r = 0.12 ).
Karantina Tumbuhan di Indonesia dalam Masa Perdagangan Bebas Nursamsi Pusposendjojo
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12116

Abstract

Plant quarantine is enacted to keep out foreign plant pests and to protect food, horticulture and estate crops from alien pests. The act prohibits or restricts entry into or passage through a country from foreign countries of plants, plant products, soil, and other materials carrying or likely to carry pests not known· to be established in the country. Experienced inspectors stationed at all points of entry into the country enforce quarantine of produce likely to introduce new pests. The importance of plant quarantine becomes more and more significant in the free trade era. Indonesia to be one of potential market for many agricultural products has still very limited number of plant quarantine inspectors. Number and quality of plant quarantine inspectors are badly needed in order enable coping the problems. Education and training should be organized and it is important to include plant quarantine in the Faculty of Agriculture curriculum.

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