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Y. Andi Trisyono
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 509 Documents
 17   18   19   20   21 
Inheritance of Resistance to Papaya Ringspot Virus-Papaya Strain in Melon (Cucumis melo L.) Budi Setiadi Daryono; Keiko T. Natsuaki`
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11966

Abstract

Papaya ringspot virus (PRSV) is one of potyviruses causing severe damage to the production of cucurbit crops including melon, however resistant melons to PRSV have not yet commercially available. To find resistant genetic source of melons against PRSV, sixty three melons were manually inoculated with PRSV-papaya strain (PRSV-P) isolated in Thailand. Levels of resistance to PRSV-P accumulation in melon leaf tissue were evaluated using a combination of visual symptom observation and RT-PCR analysis. Among melons tested, Yamatouri, Mawatauri, PI 414723, and PI 371795 showed to be resistance to PRSV-P To study the inheritance of resistance to PRSV-P, breeding experiments were conducted by crossing the resistant Yamatouri with susceptible Vakharman. The genetic analysis results revealed that a single dominant gene conferred resistance to PRSV-P in Yamatouri and its generation.
Pengelolaan Kutu Kebul (Bemisia tabaci Gen.) dengan Sistem Barier pada Tanaman Tembakau Tri Maruto Aji; Sedyo Hartono; Sri Sulandari
Jurnal Perlindungan Tanaman Indonesia Vol 19, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.16014

Abstract

Since 2009, the leaf curl disease was observed on tobacco plants grown under net shadow of PTPN X (Persero) and has caused yield losses up to 70%. The disease was likely to be associated with the existence of a high population of the sweet potato whitefly (Bemisia tabaci Gen.) and the symptoms resembled that reported for Begomovirus infection on eggplant and tomatoes. This study aimed to know the effectiveness of physical barriers in combination with a biological barrier to avoid B. tabaci in infesting the farm. The research was done by monitoring tobacco diseases to measure the diseases intensity. Research for the management of insect vectorsB. tabaci were done based on two pretexts: (1) physical barrier using the type of net; and (2) combinations of a net with a plant (corn belt). The result showed that an effective control was obtained using a net with higher mesh size rather than using a standard net with low mesh size which was presently used by PTPN X (Persero). Corn barrier did not effective to control B. tabaci except as a wind breaker.INTISARIPeningkatan populasi kutu kebul (B. tabaci Gen.) di daerah Klaten, Jawa Tengah pada tahun 2009 ternyata menjadi penyebab mewabahnya penyakit kerupuk pada tanaman Solanaceae di wilayah tersebut. Tanaman tembakau cerutu Vorstenlanden milik PTPN X (Persero) yang ditanam di area bawah naungan (TBN) yang berada di wilayah tersebut turut terjangkit wabah penyakit kerupuk setelah sebelumnya terindikasi terjadi peningkatan jumlah kutu kebul. Wabah penyakit kerupuk pada tembakau cerutu Vorstenlanden milik PTPN X (Persero) telah menurunkan hasil hingga 70%. Gejala penyakit pada tembakau berupa penyakit kerupuk. Penelitian ini bertujuan untuk mengetahui efektivitas penerapan barier fisik sebagai langkah awal untuk mengendalikan populasi kutu kebul pada pertanaman tembakau cerutu milik PTPN X (Persero). Penelitian dimulai dengan memonitoring populasi kutu kebul dan peningkatan intensitas penyakit kerupuk pada tanaman tembakau. Percobaan dilakukan secara demplot pada lahan pertanaman tembakau di wilayah PTPN X (Persero). Teknis budidaya tembakau seluruhnya mengikuti Standar Operasional Prosedur (SOP) dari PTPN X (Persero), sedangkan untuk strategi pengendalian kutu kebul dilaksanakan dengan mengikuti 2 macam perlakuan yaitu ; (1) penerapan sistem barier tunggal berupa kelambu (bermesh rapat) dan kelambu standar dari PTPN X sebagai barier fisik (2) penerapan sistem barier ganda yaitu kombinasi barier fisik dan barier biologis berupa tanaman jagung penghalang. Hasil menunjukkan bahwa penerapan sistem barier baik tunggal maupun ganda dapat menekan populasi kutu kebul sehingga berdampak pada penurunan intensitas penyakit kerupuk pada tembakau.
Ecdysone Agonist: New Insecticides with Novel Mode of Action Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 2 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.10229

Abstract

Development of insect resistance to insecticide has been the major driving force for the development of new insecticides. Awareness and demand from public for more environmentally friendly insecticides have contributed in shifting the trend from using broad spectrum to selective insecticides. As a result, scientists have looked for new target sites beyond the nervous system. Insect growth regulators (IGRs) are more selective insecticides than conventional insecticides, and ecdysone agonists are the newest IGRs being commercialized, e.g. tebufenozide, methoxyfenozide, and halofenozide. Ecdysone agonists bind to the ecdysteroid receptors, and they act similarly to the molting hormone 20-hydroxyecdysone. The binding provides larvae or nymphs with a signal to enter a premature and lethal molting cycle. In addition, the ecdysone agonists cause a reduction in the number of eggs laid by female insects. The ecdysone agonists are being developed as selective biorational insecticides. Tebufenozide and methoxyfenozide are used to control lepidopteran insect pests, whereas halofenozide is being used to control coleopteran insect pests. Their selectivity is due to differences in the binding affinity between these compounds to the receptors in insects from different orders. The selectivity of these compounds makes them candidates to be used in combinations with other control strategies to develop integrated pest management programs in agricultural ecosystems.
Seleksi, Karakterisasi, dan Reaktivitas Antibodi Monoklonal Virus Kerdil Kedelai Sri Sulandari; Y. B. Sumardiyono; Roechan M.
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12233

Abstract

Soybean Stunt Virus (SSV) is a member of Cucumber mosaic virus group that caused soybean stunt disease. The disease is the most important viral disease of soybean in Indonesia. The objective of the study was to obtain SSV monoclonal antibody for detection SSV in both infected seed and plant. Six hybridoma clones were obtained from fusion between spleen cells of BALB/c immunized with Soybean stunt virus and mouse myelome cell lines (NS-1); they were IgG3 type antibodies, and its titres were varied between 1: 1O to 1: 1OO. Using non-recoated I-ELISA method, the homolog antigen (SSV) was well detected but not the heterolog antigens (CMV isolated from banana, tobacco).
Mikovirus, Pengembangannya sebagai Agens Pengendali Hayati Supyani Supyani
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.17874

Abstract

Mycoviruses are viruses that infect fungus. In plant pathogenic fungi, mycoviruses infection may cause hypovirulence. Therefore, mycoviruses could be developed as biological control agents against the plant pathogenic fungi. Abroad, mycoviruses have long been studied and developed into biological control agents. So far, many mycoviruses have been and are being developed as biological control agents. One that has been successfully manipulated is the Cryphonectria hypovirus 1 to control blight disease on chestnut trees (Castanea dentata). In Indonesia, in brief, mycoviruses has not been sufficiently investigated. This article is aimed to introduce mycoviruses in Indonesia, as well as to inform that a study on mycoviruses has been underway in the country IntisariMikovirus adalah virus yang menginfeksi jamur. Pada jamur patogen tumbuhan, infeksi mikovirus dapat menyebabkan hipovirulen. Dengan demikian mikovirus dapat dikembangkan sebagai agens pengendali hayati terhadap jamur patogen tumbuhan. Di luar negeri mikovirus sudah sejak lama dikaji, diantaranya adalah untuk dikembangkan sebagai agens pengendali hayati. Sampai sekarang sudah banyak mikovirus yang telah/sedang dikembangkan sebagai agens pengendali hayati. Salah satu yang sudah berhasil adalah Cryphonectria hypovirus 1 untuk mengendalikan penyakit hawar kastanye (Castanea dentata). Di tanah air, mikovirus dapat dibilang belum dikaji secara memadai. Artikel ini bertujuan untuk memperkenalkan mikovirus di tanah air, sekaligus menginformasikan rintisan kajian mikovirus yang telah dilakukan di tanah air.
Hubungan Antara Inokulum Awal Patogen dengan Perkembangan Penyakit Hawar Upih pada Padi Varietas Ciherang Bambang Nuryanto; Achmadi Priyatmojo; Bambang Hadisutrisno; Bambang Hendro Sunarminto
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11724

Abstract

Study on the role of initial inoculums on disease development of rice sheath blight caused by Rhizoctonia solani Kühn was conducted in glass house at the Faculty of Agriculture University of Gadjah Mada from December of 2009 to July of 2010. The aim of this study was to evaluate the importance of sclerotia and mycelium in plant debris as primary inoculum form of R. solani. Results indicated that both sclerotia and mycelium in plant debris significantly affect the development of rice sheath blight. Disease severity was closely related to the number of sclerotia on rice plant. The highest disease severity was observed in plant inoculated with 10 sclerotia per hills. Treatments of 6 sclerotia and 5 g plant debris per hills had comparable effect on disease severity and area under disease progress curve of rice sheath blight. The results suggested that sclerotia and mycelia in plant debris might play a major role as primary inocula of the disease in rice growing field.
Resurjensi Serangga Hama Karena Perubahan Fisiologi Tanaman dan Serangga Sasaran Setelah Aplikasi Insektisida Yuni Ratna; Y. Andi Trisyono; Kasumbogo Untung; Didik Indradewa
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11766

Abstract

The use of insecticides at sub-lethal dosage may induce insect resurgence. Factors contributing the insect resurgence include mortality of natural enemies, mass migration, improvement in plant nutrition, or increase in insect reproductive capacity which works individually or in combinations. This article provides a comprehensive review from recent publications, particularly those dealing with the impact of insecticide applications on plant nutrition and insect reproduction, and function to upgrade our knowledge since most publications in Indonesia were made in the 1980s. The insecticides together with its metabolites and conjugates could function as plant growth regulators, increase absorption, improve the nutritional content, influence the biochemical and ultrastructural aspects of the plant, or protect the plant from stresses. An increase in the nutritional value of the plant would increase feeding rate, fecundity, and longevity of the insects. Furthermore, insecticides could also directly stimulate the reproductive capacity of the insect. All of these impacts might end up in increasing the population of insect after application of certain insecticides. Research findings related to the elucidation on the mechanism underlying the phenomenon of resurgence could be used in considering the registration of new insecticides or extension of the existing insecticides.
Uji Patogenisitas Bakteri Pasteuria penetrans terhadap Nematoda Puru Akar (Meloidogyne spp.) Mulyadi Mulyadi; B. Triman; Bambang R. T. P.
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 2 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12885

Abstract

The objective of the research was to study the effectiveness of P. penetrans for controlling root-knot nematodes (Meloidogyne spp.) in micro plot and in the field. The pathogenicity study in micro plot was done in Banguntapan, and field experiment was done in Ngipiksari, Pakem, Steman, Yogyakarta. The plots were arranged in Randomized Completely Block Design with three replications. The treatments used in the research were: isolat no. 2 and 3 of P. penetrans; carbofuran nematicide. and control. The results in micro plot test were: 1) root damage caused by root-knot nematodes in plot treated with P. penetrans lower than the control, and 2) percentages of parasitism of P. penetrans were 63.57 % (in isolate no. 2) and 53.46 % (in isolate no. 3). In field experiment the results showed: 1) P. penetrans found to be effective in reducing root damage caused by Meloidogyne spp. especially in 45 days old of tomato plant, whereas in 90 days old the effectiveness were decreased; 2) P. penetrans was able to grow and reproduction in the field: 3) the level of parasitisms of P. penetrans were increased rapidly during the experiment: and 4) the highest yield was found in tomato treated with carbofuran and followed by isolate 2, isolate 3, and control.
Pengaruh Kesuburan Tanah terhadap Penyakit Blendok pada Pertanaman Jambu Mete Ahmad Sulle
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11888

Abstract

Soil fertility can pressure rate attack of gummosis disease on cashew nut plantation in Muna Regency South-East Sulawesi. Blendok disease or gummosis have disseminated on some cashew plantation in Muna Regency, South-East Sulawesi. There was a correlation between soil fertilities and disease spreading. This assessment was intended to know the level attack and damage which were resulted from disease of gummosis at cashew crop. The assessment was conducted at Fongkaniwa Village of Muna Regency South-East of Sulawesi in 2004, using the rural cashew nut plantation in 30 ha area. The assessment area was divided into three blocks, i.e. high, moderate, and low level of soil fertility. Result of study indicated that attack intensity of gummosis disease on cashew nut are 19.49 %, 30.43 %, and 50.03 % on each levels of soil fertility, while Rate of Attack by the score value of 0.16, 1.14, and 1.77. Result of study indicated that improvement of soil fertility of cashew plantation are very important to pressure of attack and damage of gummosis disease.
Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Fariha Wilisiani; Susamto Somowiyarjo; Sedyo Hartono
Jurnal Perlindungan Tanaman Indonesia Vol 18, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.15602

Abstract

One of important problems of melon farming in Yogyakarta is a leaf curl disease that show specific symptom of Begomovirus infection. The little information about the nature of virus causal agent was constraint for the disease management. The purposes of this research were to molecularly identify the leaf curl causing virus in melon in Yogyakarta and to find the genetic relationship of this virus with other Begomovirus members which cause leaf curl disease. This research was conducted through several phases, which are: curly leaves collection on the field, virus DNA isolation, Begomovirus identification using universal primer Krusty Homer, Begomovirus DNA-A and DNA-B detection using primer Gemini full BamH1 forward and reverse for full genome DNA-A, and primer BF518 and BR1641 for DNA-B, genome sequencing and genetic relationship analysis of the sequence with other Begomovirus causing leaf curl. The result of field studies which were conducted in Sewon found some melon plant with symptom of Begomovirus infection. The molecular identification result using PCR showed that leaf curl causing virus in melon is Begomovirus, having DNA-A and DNA-B. Genetic relationship analysis of this virus with other Begomovirus causing leaf curl shows that this virus is closely related with Pepper yellow leaf curl Indonesia virus (AB267834) based on nucleotide and amino acid sequencing as coat protein of Begomovirus. The result of shows that the study is the first report of PepYLCIDV infection, a bipartite genome virus on melon, and its natural leaf curl symptom in Indonesia. Salah satu kendala budidaya melon (Cucumis melo L.) di Yogyakarta yaitu adanya penyakit daun keriting dengan gejala khas infeksi Begomovirus. Belum tersedianya informasi mengenai jenis dan ciri patogen virus penyebab penyakit tersebut merupakan salah satu kendala penting dalam menentukan strategi pengelolaan virus tersebut. Tujuan penelitian ini adalah mengidentifikasi secara molekuler virus penyebab daun keriting pada melon di Yogyakarta dan mengetahui hubungan kekerabatan virus tersebut dengan virus anggota Begomovirus lain penyebab daun keriting yang telah dipublikasi di database genebank. Penelitian dilakukan dengan beberapa tahapan, yaitu deskripsi gejala daun keriting di lapangan, isolasi DNA virus, identifikasi Begomovirus dengan primer universal Krusty Homer, deteksi DNA-A dan DNA-B Begomovirus dengan primer Gemini full BamH1 forward dan reverse untuk full genome DNA-A, serta primer BF518 dan BR1641 untuk DNA-B, sequencing genom, dan analisis hubungan kekerabatan sequence tersebut dengan Begomovirus lain penyebab daun keriting. Hasil pengamatan lapangan di Sewon Bantul diperoleh tanaman melon dengan gejala khas infeksi Begomovirus. Hasil identifikasi secara molekuler dengan PCR menunjukkan bahwa virus penyebab daun keriting pada melon adalah Begomovirus, memiliki DNA-A dan DNA-B. Analisis hubungan kekerabatan virus penyebab daun keriting pada melon dengan Begomovirus lain penyebab daun keriting menunjukkan bahwa virus tersebut berkerabat dekat dengan Pepper yellow leaf curl Indonesia virus (AB267834) berdasarkan sekuen nukleotida dan asam amino sebagian coat protein Begomovirus. Hasil penelitian ini menunjukkan bahwa penelitian ini merupakan laporan pertama infeksi PepYLCIDV dengan bipartite genome pada melon dengan gejala daun keriting secara alamiah di Indonesia. 

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