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Contact Name
Y. Andi Trisyono
Contact Email
anditrisyono@ugm.ac.id
Phone
+62274-523926
Journal Mail Official
jpti.faperta@ugm.ac.id
Editorial Address
Jalan Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Jurnal Perlindungan Tanaman Indonesia
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Arjuna Subject : -
Articles 509 Documents
Appropriate Number of Sex Pheromone Trap for Monitoring Spodoptera exigua Hubner (Lepidoptera: Noctuidae) Moths on Shallot Field Dian Lestari; F. X. Wagiman; Edhi Martono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.23370

Abstract

The research was carried out to determine the appropriate number of sex pheromone traps for monitoring the Spodoptera exigua moths in shallot crop which was grown in Yogyakarta Special Territory, Bantul Regency (Samas). This research was conducted in August until September 2016. Sex pheromone trap was arranged on shallot aged  3 weeks after planting. Sex pheromone trap with hexadecenat tetra asetat ingredients was placed on sticky yellow trap. This research  used RCBD experimental design with single factor experiment namely the number of trap i.e. 3, 5, 6, 8 units/2.000 m2 with sex pheromone, and  three traps without sex pheromone as control. Each treatment was repeated 5 times so  125 samples of experiments were obtained. Observation was made by counting the number of moths caught in sex pheromone trap. The result of observation was analysed  with analysis of variance (ANOVA) and DMRT α 5.0%. The results show that the appropriate number of trap to monitor the pest infestation was 3 units/2.000 m2.
Responses of Capsicum annuum Varieties toward Root Knot Nematode Meloidogyne incognita Infection Resty Islamiati Putri; Siwi Indarti; Ani Widiastuti
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.23978

Abstract

Chili pepper (Capsicum annuum) is one of the major vegetable commodities in Indonesia. One of the challenges in chili pepper cultivation is the infection of Meloidogyne incognita which causes gall formation in root systems. The purposes of this research were to determine 1. the response of four varieties of chili pepper against M. incognita infections; 2. damaged intensity caused by this pest in the root system, and; 3. development of M. incognita populations in root systems (rhizosphere). This research was conducted in Plant Pest Science Laboratory and Greenhouse of the Department of Crop Protection, Faculty of Agriculture, University of Gadjah Mada. This research included cultivation of three chili pepper varieties (Cabai Merah Besar, Pilar F1; Cabai Merah Keriting, Kastilo F1; and Paprika, Red Star 2060), rearing of M. incognita, inoculation, and observation. Chili pepper respond to M. incognita infections were evaluated based on agronomic aspects of the plants, such as root histopathology observation, disease intensity, and nematode populations. Results showed that each variety of chili pepper expressed various responses to infections. All varieties had different root weight and length, but had similar sensitivity based on the appearance of root damage and egg mass scoring of M. incognita. Although nematode population could develop on roots, observation from root tissues showed lignification after infection of J2 M. incognita. Chili pepper var. Red Star 2060 was more susceptible against M. incognita than chili pepper var. Pilar F1 and chili pepper var. Kastilo F1.
Responses of Tomatoes Grafting Using Variation of Rootstock against Virus Infection and Tomato Yields Tri Retno Widyastuti; Sri Sulandari; Sedyo Hartono; Triwidodo Arwiyanto
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.31056

Abstract

Grafting methods on tomato have been done to reduce the infection rate of various pathogens. Begomovirus and Crinivirus are important viruses in tomato plants. The research aimed to determine the resistance response of tomato plants to viral infection, and tomato production. Field research was conducted in Harjobinangun, Pakem, Sleman, Yogyakarta in the endemic area of the viral diseases transmitted by Bemisia tabaci. This experiment used a Completely Randomized Design non-factorial with “Servo” as scion and “Amelia”, “H-7996”, “Mawar” as rootstock. The disease development, presence of viral diseases, and tomato yields were observed. PCR detection using Krusty & Hommr primer successfully amplified Begomovirus DNA bands with an approximate size of 580 bp in tomato plant with interveinal chlorosis, curling, thick, rigid, and stunt symptoms. Chlorotic spots and yellowing symptoms successfully amplified using ToCV-CF/ToCV-CR specific primer for the amplification of Tomato chlorosis virus with DNA band approximately size of 360 bp, whereas using TICV-CF/TICV-CR specific primer could not amplify the virus cDNA. The leaves roll upward with purple interveinal symptoms that were not infected by both viruses. Both viral infections affected the quality of the fruit which indicated by a higher number of abnormal fruits. “Servo” grafted onto “Amelia” and non-grafted Servo were tolerant to viral infection, “Servo” grafted onto “H-7996” or to “Mawar variety were susceptible to viral infection, self-grafted Servo were very susceptible to viral infection. 
Molecular Identification of Begomovirus Infecting Angled Luffa Alvina Clara Giovanni; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.31073

Abstract

Begomovirus was reported as one of the most aggressive and destructive viruses on several commercial crops, including cucurbits in Indonesia. Plants that infected with Begomovirus show the mosaic symptom on the leaves, change in leaf shape, stunts, change in color and shape of fruit. It was recently observed in cultivated angled luffa [Luffa acutangula (L.) Roxb] around Yogyakarta and Central Java. The aim of this research was to identify the virus by using Polymerase chain reaction (PCR). The result of Begomovirus amplification from the total DNA samples amplification using primer Krusty-Homer showed that DNA of Begomovirus from angled luffa was amplified at ~580bp. The DNA sequencing of angled luffa’s leaf isolate GD1 had 97.8% homology with SCLV-China isolate MC1. However, amplification of DNA seed samples using the same primer showed negative result. It was concluded that Begomovirus was not a seed borne virus. This is the first molecular report on the occurence of Begomovirus in angled luffa in Yogyakarta.
Efficacy of Sulfuryl Fluoride as a Fumigant against Aspergillus niger on Corn Seeds Leny Panjaitan; Salbiah Salbiah; Ranta Hadi; Abdurakhman Abdurakhman; Mustopha Ahad
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.34822

Abstract

The importation of corn seeds may inadvertently introduce seed-borne fungi like Aspergillus niger. One potential method to control this pathogen was fumigation with sulfuryl fluoride. The objectives of this applied research were to obtain effective concentration and exposure time of sulfuryl flouride as a fumigant to control seedborne fungi and to its physiological impact on corn seeds. Aspergillus niger was isolated from corn seed samples, grew it in potato dextrose agar, and used it for in vitro studies. Parameters  observed were isolates’ growth inhibition. In vivo studies were conducted using corn seed samples infected by Aspergillus niger. Sulfuryl fluoride was applied at the concentration of 30, 40, 50, and 60 g/m3 and 24, 48, 72, and 96 hour of exposure time. Results showed that sulfuryl fluoride concentration of 40 g/m3 for 48 hours at 26-32°C is the only effective concentration against A. niger in vitro but all of the treatment did not significantly affect A. niger in vivo. Concentration of 30, 40, 50, 60 g/m3 for 24 hours does not affect the quality of the seed. 
Weed Seed Species Inventory of Wheat Imported through Tanjung Perak Port Ulya Wildaniyah; Rahma Susila Handayani
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.42159

Abstract

Wheat seeds importation has the potential of weed seeds that can harm Indonesia's biodiversity. This study aims to determine the types and morphological characters of weed seeds carried by imported wheat from Australia, Canada, and Ukraine through the port of Tanjung Perak-Surabaya for January-June 2018. The research was started by collecting weed seeds from wheat samples, observing using a SMZ 1270 stereo microscope and identifying them using a reference book. The weed seeds were successfully identified as 13 families with 20 species originating from Australia, Canada and Ukraine. The most common types of weeds are from the Poaceae family. Weed this family are often found in the form of florets, the seeds of which are called caryopsis are coated in lema and palea, as in grains. Weed seed species found in wheat commodities from the three countries, are: Avena fatua, Brassica campestris, and Polygonum convolvulus, are not classified as quarantine pest.
In Vitro Cultivation of Aphelenchoides besseyi Christie on Fungal Cultures Annisa Nur Imamah; Supramana Supramana; Tri Asmira Damayanti
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.42227

Abstract

White tip disease on rice caused by Aphelenchoides besseyi is categorized as emerging infectious disease in Indonesia because the disease incidence and its geographical distributionare increasing rapidly. Until presently, there is no information related with mass culture technique of Ap. besseyi in Indonesia. Thus, studies to obtain pure population of Ap. besseyi  are necessary to be conducted to support various research aspects in the future. This research is aimed to study Ap. besseyi pure mass culture technique by rearing the nematode on three fungal species. Three different fungal cultures on PDA medium, i.e. Alternaria padwickii, Fusarium semitectum, and Botrytis cinerea were tested as Ap. besseyi cultivation media. To obtain the optimum condition for nematode cultivation, the fungal cultures were incubated in three different temperature. The nematodes were surface sterilized with 0.1% streptomycin sulfate followed by washing with sterilized water before infesting the nematode into fungal culture. Subsequently, 25 sterilized nematodes were infested into 7 days old of fungal culture and incubated at 20ºC, 25ºC and 30 ºC. After 21 days, nematodes were harvested and counted the final population. Among fungal species tested as rearing medium, the best culture for Ap. besseyi reproduction was on Al. padwickii culture at 25 ºC with average final population up to 9,115 per petri dish and reproduction factor 364.6. At a 30ºC, Ap. besseyi was failed to develop its population in all the fungal cultures tested. It indicated that the optimum temperature for reproduction  of Ap. besseyi on those of  fungal  species was 20-25 ºC.
In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani Wawan Setiawan; Suryo Wiyono; Efi Toding Tondok; Atit Kanti; I Made Sudiana
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.43344

Abstract

Rhodotorula sp. is widely known as a biocontrol agent and is reported effective in controlling several diseases on crops. Rhodotorula minuta Dmg 16 BEP is an antagonist yeast from Indonesia which was effective in controlling various plant diseases. The objective of this research was to study the in vitro mode of action of R. minuta Dmg 16 BEP against Alternaria solani. The antibiosis ability of R. minuta Dmg 16 BEP to A. solani might be based on the activity of volatile compounds and not from its metabolites products. The growth of A. solani in paper disk assay and dual culture tests were not inhibited nor inhibitory zones  developed, whereas the inverse petridish test showed the growth inhibition of A. solani. The results of the chitinolytic activity test on chitin agar and proteolytic on skim milk agar showed that no clear zone was formed. R. minuta has a strong hyperparasitic ability according to the slide culture test as the yeast cells surrounded A. solani hyphae and caused damage to some parts of hyphae.
Feeding Inhibition by Chitosan on Larvae of Spodoptera litura (Lepidoptera: Noctuidae) Sulistia Ningsih; Nugroho Susetya Putra; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.44032

Abstract

The high usage of synthetic insecticides for controlling Spodoptera litura could be detrimental to the environment, especially on non-target organisms. Therefore, more environmentally friendly pest management techniques should be used, for example, using a natural product such as chitosan. The objective of this study was to understand the effect of feeding inhibition by chitosan on the feeding activity of the third instar larvae of S. litura. The feeding inhibition test was carried out using the choice methods in a factorial completely randomized design (CRD) with seven treatments: i.e. chitosan 5×103, 15×103, 25×103, 35×103, 45×103 ppm and profenofos 0.18×103, 0.34×103 ppm, and one control treatment. The test of the choice methods was carried out by three larval laying positions: (1) between the control and treatment feeds (Position A), (2) above the treated feed (Position B), and (3) above the control feed (Position C), and were replicated three times. The non-choice test was done in a completely randomized design (CRD) with seven treatments plus control and were replicated four times. The results showed that the chitosan in the concentration range of 5×103‒45×103 ppm reduced feeding by S. litura larvae by 2.587 to 34.974% in the choice method, and 11.610 to 50,712% in the non-choice method. This feeding inhibition increased significantly with the increment of chitosan concentration. However, the inhibition effects by chitosan was weaker than the inhibition by profenofos LC50 in both tests at a concentration of 0.34×103 ppm: 44.331% and 62.491% respectively. In conclusion, chitosan with a concentration of 45×103 ppm at all larval laying positions showed the highest value of feeding inhibition activity on the third instar larvae of S. litura compared to other chitosan concentrations in both methods.
The Role of Nanochitosan on the Expression of Rice Resistance Genes against Bacterial Leaf Blight Siswanti Siswanti; Tri Joko; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.44418

Abstract

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has been reported to cause ± 20–50% of rice yield loss around the world. Resistant varieties are used to control this disease, however due to rapid evolution of this pathogen, the resistances was broken down in a few years. This study is aimed to determine the role of nanochitosan in the expression of rice Xa21 and Xa1 resistant genes against Xoo. The BLB susceptible rice cultivar IR64, the Xoo isolate MAG2 and a 0.065% concentration of nanochitosan were used in this experiment. Application of nanochitosan was carried out within 1-week intervals starting at rice aged 2–10 weeks after transplanting. The expression of Xa21 and Xa1 genes against Xoo were analyzed using conventional PCR and qPCR methods at 0 and 4 days after Xoo inoculation followed by 4x scoring of disease symptoms in 1-week interval. The treatments used in this study included the mock one/inoculated with sterile distilled water, K (+)/ plants inoculated with Xoo, CNP (-)/ with nanochitosan and sterile distilled water inoculation, and CNP (+)/ with nanochitosan and Xoo inoculation. The results showed that the 0.0065% concentration nanochitosan application was able to increase the expression of Xa21 and Xa1 genes on CNP (-). Disease intensity and AUDPC values did not show any significant difference between K (+) and CNP (+). This study concluded that nanochitosan at 0.065% was able to increase the expression of rice Xa21 and Xa1 resistance genes. However, the gene expression was not able to significantly suppress the infection development of Xoo.