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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,001 Documents
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Apigenin, an Anticancer Isolated from Macaranga gigantifolia Leaves Sofa Fajriah; Megawati Megawati; Akhmad Darmawan
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.02

Abstract

Apigenin, a flavonoid compound has been isolated from the ethyl acetate fraction of methanol extract of Macaranga gigantifolia leaves. Isolation and purification of apigenin conducted using chromatographic method, and chemical structure characterized based on spectroscopic data. In vitro anticancer activity test against murine leukemia P-388 cell line showed potential activity with IC50 14.13 μg/mL.Key words: apigenin, flavones, Macaranga gigantifolia, anticancer, murine leukemia P-388 cell line.
Spatial Variability of Fruit and Seedling Growth of Mindi (Melia azedarach L.) in Community Forest, West Java, Indonesia Laswi Irmayanti; Iskandar Zulkarnaen Siregar; Prijanto Pamoengkas
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.09

Abstract

Mindi (Melia azedarach L.) is mostly found in community forests of West Java. One of the important factors affecting productivity of community forests is the use of quality seed. Due to scarcity of seed sources, however, seed quality is not easily available. Currently, community forests serve also as ad hoc seed sources that are usually without appropriate quality assessment. This research was aimed to determine baseline information on spatial variability of mindi fruits collected from five seed sources, and seedling growths treated in seven different growing media consisting mixtures of soil, rice husk, sand and cattle manure. Five mindi populations were selected in the following villages: Nagrak (Bogor), Babakan Rema (Kuningan), Padasari (Sumedang), Sukakarya (Bogor) and Gambung (Bandung). The results showed that variability of seed dimension (CV=4.82-14.37%) and seedling growth (11.79-21.77%) were moderate. The mindi fruits from Nagrak were found to be the biggest in terms of size (1.22 ± 0.17 cm), weight (0.40 ± 0.04 g), and seed set (85.47 ± 15.09%). In addition, the seedling growth from Nagrak was also better than those of other villages showing its potential for future seed sources. Growing media containing cattle manure were observed consistently to be the best for seedling growth. The findings are useful in supporting scientifically the selection of seed sources from community forests.
The effect of Nigella Sativa extract on Alpha-ketoglutarate activity and histopathologic changes on Rat liver induced by Monosodium glutamate Ala Shukri Eshami; Karyono mitaroem; Hidayat Sujuti; Abdussalam Ashour
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.03

Abstract

         Monosodium glutamate (MSG) is a commonly used food additive and found in most soups, fish and processed meat. The use of MSG in food is growing. Irrational fear had increased in the last few years due to the adverse reactions and toxicity of MSG, which effect on the liver.  Nigella sativa is used as traditional medicine for the treatment of many diseases, it has been extensively investigated in recent years, traditional medicine for the treatment of many disease due to its notable pharmacological properties. NS can inhibit oxidative stress. The present study was undertaken to investigate different Doses of Nigella sativa on alpha KGDH activity and liver histology of MSG induced rat. The animals (n=30) Were grouped  A as (control), B treated with MSG 1g/kg, C MSG treated with NS 0.01g/kg, D MSG treated with NS 0.02g/kg, E MSG treated with NS 0.04g/kg and F treated with NS 0.02g/kg then we investigated the effect alpha KGDH activity by ELISA method and liver histopathology by light microscope.  alpha KGDH activity were significantly increased in treatment MSG compared with treatment negative control, MSG + NS 0.1g/kg, MSG + NS 0.02g/kg, MSG + NS 0.04g/kg, and NS 0.02g/kg. The histological changes in group B and C showed disturbed liver architecture, hemorrhage in the central veins, areas of necrosis, vacuolation and increased inflammatory cells infiltration while group D showed central vein is normal and vacuolation of cytoplasm, group E showed more normal liver architecture as shown less vacuolation in cytoplasm, central vein is normal, and group F showed no pathologic changes.These finding showed that administration of MSG increase alpha KGDH and induced damage in liver tissue. Nigella sativa extract can reduce alpha KGDH and prevent liver damage induced by MSG.Keywords :  Monosodium glutamate, Alpha-ketoglutarate Dehydrogenases, Nigella sativa and liver damage.
Correlation between Serum Ferritin and Cardiac Troponin I in Major Beta Thalassemia Children Muhammad Ali Shodikin; Renny Suwarniaty; Susanto Nugroho
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.03

Abstract

Background: Major beta thalassemia (MBT) is a hereditary disease which synthesis defect on beta chains of haemoglobin, it is cause red blood cell destruction and the symptoms of anemia. Red blood cells destruction, frequent blood transfusion and low adherence to routine use of iron chelator were cause iron accumulation in the heart, liver and endocrine organs. Accumulation of iron in the myocard can lead acute myocardial infarction. One of cardiac markers that had been used for the diagnosis of myocardial infarction was cardiac troponin I (cTnI). The aim of this research is find correlation between serum ferritin levels and cTnI in MBT children.Methods: A descriptive analytic research was conducted using a cross sectional design. The subjects were divided into 2 groups, the MBT group and the control group. In both groups perform laboratory examination to checks the serum ferritin and cTnI levels. Data were analyzed using t-test and Pearson correlation test.Result: Eleven children in the MBT group and 11 children in the control group were involved in this study. In the MBT group, the mean of serum ferritin and cTnI levels were 4292.5 μg/L and 0.22 ng/mL respectively. In the control group, the mean of serum ferritin and cTnI levels were 136.2 μg/L and 0.20 ng/mL respectively. The mean of serum ferritin levels in MBT group was higher than control, statistically significant (p= 0,0004 ). The mean of serum cTnI in MBT group was higher than control, statistically not significant (p= 0,82). In MBT group, there was a weak corellation between serum ferritin and cTnI levels (r = 0,34). Keywords: Major beta thalassemia, children, ferritin, cTnI
Modulation of Granulocyte Cells Development by VipAlbumin® Administration in BALB/C Mice with Diabetes Mellitus Andi Rizki Adi Pradana; Mansur Ibrahim; Muhammad Sasmito Djati; Muhaimin Rifa'i
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.05

Abstract

Diabetes mellitus is a metabolic disease that is caused either by the decrease of insulin secretion frompancreatic β cells or the insensitivity of target cells against insulin. High glucose levels (hyperglycemia condition)can trigger the formation of free radicals, the main cause of diabetes micro and macrovascular complications. Theformation of free radicals and AGE (advanced glycation end-products) is assumed to became the key factor in thedecline of granulocyte cell production as well as the disruption of these cells functional activity. The purpose ofthis research was to determine the role of VipAlbumin® in inhibiting the adverse effects of increased blood glucoselevels, which highly influence the production of granulocyte. This study was divided into in vitro and in vivostage. BALB/C mice were used as experimental animals at in vivo stage and induced to undergo diabetes through100 mg/kg BW streptozotocin (STZ) injection at the age of 5 days. VipAlbumin® administered orally for 14 days,which began when mice reached the age of 14 weeks. The administration of VipAlbumin® divided into 3 dosesi.e. 0,01664 mg/gr BW (1st dose), 0,416 mg/gr BW (2nd dose), and 10,4 mg/gr BW (3rd dose). The further step wasa flowcytometric analysis to see the development of granulocyte cells relative amount, which were isolated fromthe bone marrow. The result of this analysis shows that VipAlbumin® administration, particularly at the 2nd and3rd dose, were able to modulate granulocyte cells development in the bone marrow.
Genetic Diversity of Porang Populations (Amorphophallus Muelleri Blume) In Central Java and West Java Based on LEAFY Second Intron Marker Isna Arofatun Nikmah; Rodiyati Azrianingsih; Didik Wahyudi
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.05

Abstract

Porang (Amorphophallus muelleri Blume) dispersed and grows well in Java island. This research aimed to determine the genetic diversity of porang populations in Central Java and West Java based on LEAFY second intron (nuclear genome encoding flower development). DNA samples of porang were from previous study, which are obtained from Central Java (Pamedaran, Grobogan, Wonogiri, Karangtengah) and West Java (Cisompet) as ingroup. A. variabilis from Pamedaran Brebes, A. ochroleucus, A. longituberosus, A. sumawongii, and A. tinekeae from genbank were treated as outgroup. LEAFY second intron was amplified using primers FLint2 F1 5’-CTTCCACCTCTACGACCAGTG-3’ and FLint2 R1 5’-TCTTGGGCTTGTTGATGTAGC-3’. Analysis using dnasp5 showed that 23 samples had 89 invariable sites and 139 variable sites that consisted of 12 singleton two variants sites, 118 parsimony-informative two variants sites and 9 Parsimony-informative three variants sites. The total of haplotype were 7 haplotypes where 16 ingroup samples clumped on haplotype 1 whereas 2 samples of Grobogan (Grobogan I and Grobogan III) clumped on haplotype 2 and outgroup samples occupied at their own haplotype. Genetic distance of all ingroup samples were 0-0,004 showing no differences among individuals. Analysis of phylogenetic tree using Maximum Parsimony, Neighbor Joining and Maximum Likelihood resulting a polytomy branch of ingroup samples except Grobogan which forms a separated sub clad. Key words: Genetic diversity, haplotype, Java, LEAFY, Porang.
Effect of Nigella Sativa Extract on Inflammatory Cells, Interleukin-10, Interferon-γ and Histological of Kidney in Monosodium Glutamate-Induced Rats Abdalrauf A Mahmud Yousif; Hidayat Sujuti; Edi Widjajanto
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.04

Abstract

There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), a flavor enhancer was unhealthy. Herbal medicine Nigella sativa (NS) has antioxidant properties able to cure the toxic induced by MSG. This study aimed to evaluate the risks of excessive use of MSG and to study the role of NS to inhibit inflammation and renal damage. Treated rats (twenty four male wistar rats) were divided into six group and analyzed by measuring the cells in blood, interleukin-10, interferon-γ serum levels by ELISA method and remove kidneys for histological examination. Histological of kidney for all groups except control, were showed different abnormalities include congestion of some blood vessels, hemorrhage  between tubules, widening in the renal tubules, revealed severe dilatation of Bowman's capsule and shrinkage of glomeruli, and areas of huge vacuole, were observed compared with control. Interleukin-10 was reduced in Groups 2,3,4 and 5, whereas increase in NS group compared with control. Interferon-γ was increased in groups 2,3,4 and reduced in groups 5,6 compared with control.  Eosinophil was increased in groups 2,5 and reduced in groups 3,4, 6 compared with control. This present study showed that administration of MSG to rats induced many changes effects on inflammatory cells, cytokines and histological of kidneys. NS has benefit in blood parameters, whereas harmful on kidney at these doses.
Genomic and Haplotype Comparison of Butanol Producing Bacteria Based on 16S rDNA Ekwan Nofa Wiratno; Suharjono Suharjono; Agustin Krisna Wardani
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.01

Abstract

High butanol demand for transportation was support to butanol development. Exploration of butanol producing bacteria using genome comparison and biogeography give role to butanol industrialization. Objective of this research are butanol production, genome comparison and haplotype analysis of butanol producing bacteria from Ranu Pani Lake sediment using 16S rDNA sequences. Highest butanol concentrations were resulted by Paenibacillus polymyxaRP 2.2 isolate (10.34 ± 0.00 g.l-1) then Bacillusmethylotrophicus RP 3.2 and Bacillusmethylotrophicus RP 7.2 isolate (10.11 ± 0.01 g.l-1 and 9.63 ± 0.01 g.l-1). Number of bases (T, C, A, G) of group 1 are similar, but different with group 2. Least G+C content is Clostridium saccharobutylicum Ox29 (51.35%) and highest is Bacillus methylotrophicus RP 7.2. Conserve region (1044 bp) of 16S rDNA higher then variative region (367 bp). The number of 319 bp is PIS whereas single tone as much as 48 bp. There are 17 conserves sequences. All of butanol producing bacterial sequences was clustered to 8 haplotype. Based on source of bacteria, there are three group of haplotype. Group A was isolated from Asia, group B was isolated from America and group C was isolated from Europe.
Kinetic Parameters Analysis of Liver and Kidney Catalase Under The Influence of Cadmium and Mercury In Vitro Ellsa Anggun Karantika; Supianur Supianur; Edyson Edyson; Eko Suhartono
Journal of Tropical Life Science Vol. 6 No. 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.01

Abstract

Cadmium (Cd) and mercury (Hg) are toxic metals that affect human organs function, including liver and kidney. This toxic activity is because the heavy metal could induce oxidative stress and interfere antioxidant activities, including catalase (CAT). The present study was aims to evaluate the effect of Cd and Hg to liver and kidney CAT kinetic parameters in vitro. In this experiment, liver and kidney were taken from male rats (Rattus novergicus). Sample the homogenized and divided into three groups with; T0 served as control which contains liver or kidney homogenate + H2O2, T1 which contains liver or kidney homogenate + H2O2 + 0.03 mg/L CdSO4; and T2 which contains liver or kidney homogenate + H2O2 + 1 mg/L Hg. Solutions then incubated at 37ºC for 1 hour and then was prepared for CAT activity measurement. The CAT activity was measured using spectrophotometer at 240 nm. For measuring the kinetic parameters, different concentration of H2O2 were used. The kinetics parameters (Km and Vmax) were calculated using Lineweaver-Burk plot. The results shows that Cd and Hg could decrease the affinity of CAT-H2O2 complex which expressed by the higher Km and Vmax values. Also from the results, Cd has better activity to decreased the affinity of CAT-H2O2 complex than Hg. From this results, it can be concluded that Cd and Hg treatments could inhibit CAT activity in liver and kidney in vitro.
Evaluation of Indigenous Potato Challisha (Solanum tuberosum L. Cv. Challisha) Somaclonals Tolerance to Salinity In Vitro Md. Sanaullah Biswas; Md. Riazul Islam; Mohammad Zakaria
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.13

Abstract

Potato is one of the most important food crops in the world. It is generally sensitive to salinity and likes to grow in neutral soil. On the other hand, salinity is increasing alarmingly in the ever changing climatic conditions. Thus, the selection of salt tolerant potato cultivars is necessary to keep pace the production of potato. To select salt tolerant cultivars, here we attempt to compare the salinity level between indigenous and modern cultivars. In vitro selection of local and modern potato cultivars were investigated with five levels of NaCl (0, 30, 60, 90 and 120 mM). The indigenous potato Challisha and modern cultivars Diamant and Felsina were used as plant materials. Significant differences were noticed among the cultivars in response to different levels of NaCl. Plant growth and root development were gradually reduced with increased concentration of NaCl. All three cultivars were survived well with exhibiting different growth status up to 60 mM NaCl, but they performed poorly at 120 mM of NaCl. Cultivar Challisha performed better regarding shoot length, root length, the number of nodes per plantlet and the fresh weight per plant up to 90 mM of NaCl. Thus, we can conclude that local indigenous variety Challisha is salt tolerant comparing with the modern cultivated varieties.

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