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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,002 Documents
Natural Deep Eutectic Solvent–Extracted Old and Young Mangrove Avicennia marina Leaves: Comparative Antibacterial Activity Against Propionibacterium acnes Djamaludin, Heder; Firdaus, Shelina Innasa; Suprayitno, Eddy; Yahya, Yahya; Arisandi, Desy; Ahmad, Mirza Gulam; Tambunan, Jeny Ernawati; Ahmad, Nurul Hawa; Ramli, Nurul Shazini; Kartikaningsih, Hartati
Journal of Tropical Life Science Vol. 15 No. 3
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.03.13

Abstract

Acne vulgaris is a common inflammatory skin disorder largely associated with the proliferation of Propionibacterium acnes. Although antibiotics are widely used for acne treatment, the increasing incidence of antibiotic resistance necessitates the exploration of effective natural alternatives. Mangrove plants are known to contain diverse bioactive compounds with antimicrobial potential. This study aimed to evaluate the antibacterial activity of Natural Deep Eutectic Solvent (NADES) extracts derived from young and old leaves of Avicennia marina against P. acnes. Extraction was conducted using citric acid–glucose-based NADES with different molar ratios: P1 (1:1), P2 (2:1), P3 (3:1), and P4 (4:1), to assess the effect of solvent composition on antibacterial performance. Antibacterial activity was determined using inhibition zone assays, while molecular docking analysis was performed to investigate interactions between extracted metabolites and P. acnes target proteins. The results showed that both young and old leaf extracts exhibited significant antibacterial activity, strongly influenced by the NADES formulation. Young leaf extracts produced the largest inhibition zone with the P4 formulation (24.51±0.75 mm) and the smallest with P1 (21.63±0.67 mm). Similarly, old leaf extracts showed maximum inhibition in P4 (37.38±4.34 mm) and minimum inhibition in P1 (22.62±1.40 mm). Increasing the proportion of citric acid enhanced the extraction of bioactive compounds responsible for antibacterial activity. Molecular docking confirmed strong binding affinities between key metabolites and P. acnes target proteins, supporting the experimental findings. Thus, NADES extracts of A. marina leaves, particularly those prepared using a 4:1 citric acid–glucose ratio, demonstrate strong potential as natural antibacterial agents for acne prevention and treatment.
Integrated Bacterial and Fungal Microbiome Profiling of Green Honey from Banggi Island, Sabah Ullah, Saeed; Huyop, Fahrul Zaman
Journal of Tropical Life Science Vol. 15 No. 3
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.03.15

Abstract

Green honey from Banggi Island, Sabah, is a premium product recognized for its distinctive color, yet its microbial composition remains poorly characterized. This study employed amplicon sequencing to investigate the microbial diversity of green honey, targeting the bacterial 16S rRNA V3 region and the fungal internal transcribed spacer one (ITS1) region. A total of 286 amplicon sequence variants (ASVs) were identified, comprising 270 bacterial and 16 fungal/yeast ASVs across 12 bacterial and 2 fungal phyla. The bacterial community was dominated by members of the phylum Pseudomonadota, with representative genera including Comamonas, Acidovorax, and Bacillus. Fungal profiling revealed a strong dominance of Zygosaccharomyces rouxii, accounting for over 98% of fungal reads. Overall, fungal sequencing reads substantially exceeded bacterial reads, indicating a fungal-enriched microbial profile in this honey sample. These findings provide the first integrated characterization of both bacterial and fungal communities in Banggi Island green honey. While the results highlight distinct microbial patterns potentially shaped by local environmental and floral factors, functional, safety, and bioactivity implications remain exploratory and warrant further investigation using complementary approaches.

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