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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,001 Documents
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The Role of Black Soybean and Purple Sweet Potato Active Compound on Advanced Glycation End-Product in Streptozotocin-Induced Type 2 Diabetes Mellitus Rat Abdul Gofur; Siti Nur Arifah; Yuslinda Annisa; Agung Witjoro; Mochammad Fitri Atho'illah; Sri Rahayu Lestari
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.02

Abstract

Diabetic nephropathy is one of the diabetes complications attacking kidney leading to kidney damage. Hyperglycemia accompanying DM causes the increase of Ad-vanced Glycation End-Product (AGE) and Receptor Advanced Glycation End-Product (RAGE) activity, then develop kidney damage and other diabetes compli-cations. The study aimed to investigate the effect of black soybean, purple sweet potato, or their combination on the expression of AGE, RAGE, and kidney necro-sis T2DM model rats. The rats were given with high-calorie diet for five weeks and then injected with a low dose of streptozotocin (30 m/kg Body Weight) in intraperitoneal. DM rats were divided into: normal, K- (T2DM), K+ (T2DM + glibenclamide 0.6 mg/kg body weight), P1 (T2DM + black soybean), P2 (T2DM + purple sweet potato), and P3-5 Combination 1-3 (T2DM + combination of black soybean and purple sweet potato in ratio of 1 : 3, 2 : 2, and 3 : 1). DM rats were then given the treatments for thirty days. The effect of black soybean, purple sweet potato, or the combination of both was evaluated through the expression of AGE, RAGE, and necrosis of renal tubules. The changes in renal tubules histological characteristics were evaluated using hematoxylin-eosin (HE) staining. Immuno-histochemistry analysis of renal tubules was to evaluate AGE-RAGE expression after the treatments. The research results indicated that there was a significant dif-ference from the combination of black soybean and purple sweet potato in reduc-ing AGE, RAGE, and renal tubules necrosis. The BSB and PSP combination ratio of 1:1 was able to improve renal tubules, decrease the expression of AGE and RAGE towards near normal. The combination of black soybean and purple sweet potato could be used as one of the alternatives to improve kidney damage in dia-betic nephropathy.
Floristic and Phytoclimatic Study of a Sacred Grove Vegetation of West Midnapore District, West Bengal, India Uday Kumar Sen; Ram Kumar Bhakat
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.01

Abstract

Traditional cultural and religious beliefs and practices play a crucial role in the conservation of the environment and biodiversity in the form of the sacred groves. The present study was carried out to explore the plant resources, their biological spectrum, leaf size spectra, the conservation status of a sacred grove, locally known as Narampur Barapir Astana (NBA) in West Midnapore district of West Bengal in India. Floristic list of the study revealed that the vegetation of NBA was diverse and composed of 277 species belonging to 238 genera distributed over 77 families under 36 orders according to APG IV classification. The dominant order and family in terms of species richness were Lamiales 27 (9.75%) and Fabaceae 32 (11.55%) respectively. The biological spectrum shows the study area was classified as “thero-chamae-cryptophytic” type of phytoclimate. As regards the leaf size spectra, mesophyll 56 (20.22%) was found to be high. The study area being a sacred grove is fairly undisturbed. For the long-term conservation of germplasm of the grove some recommendations are suggested.
New Host Record of Lutjanus gibbus for Parasitic Nerocila sp. (Crustacea, Isopo-da, Cymothoidae) from South Andaman, Andaman Islands, India Pathan Anil; Paul Sayeri; Kadeparambil Arjunan Jayaraj
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.03.01

Abstract

A new host of Cymothoid isopod, Nerocila sp. was identified from the dorsal surface of the body (caudal peduncle) of Lutjanidae fish Lutjanus gibbus (Forsskal, 1775) which is a new host record of isopod parasite Nerocila sp. from the coastal waters of South Andaman, Andaman Islands. This study revealed the first occurrence of this parasitic isopod (Nerocila sp.) from Andaman and Nicobar Islands. A comprehensive summary of the nominal Nerocila species recorded on different host species is provided. The present finding adds another species of isopod to the checklist of Andaman and Nicobar Islands.
Potentials of Hydrocarbon Utilization by Wood-Digesting Bacteria isolated from the Lagos Lagoon, Nigeria. Olanike Maria Buraimoh; Adewale Kayode Ogunyemi; Isaac Edward Oyegbite; Olukayode Oladipo Amund; Matthew Olusoji Ilori
Journal of Tropical Life Science Vol. 10 No. 1 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.01.01

Abstract

Introduction: There is a continuous search for environmental–friendly methods to address oil-polluted environments, hence this study aimed to establish the potential of using tropical estuarine bacterial strains for degradation of crude oil in polluted environment. Microbial degradation of hydrocarbons has been considered a promising, natural, less toxic and cost-effective technology. Lignocellulose-utilizing bacterial strains (Bacillus megaterium strain NOB, Streptomyces pseudogriseolus strain EOB and Paenibacillus sp. strains ROB) were isolated from decomposing wood residues in a tropical lagoon. They were identified on the basis of morphological and biochemical characteristics, observation under scanning electron microscope and 16S rRNA gene sequencing. Methods; Microbial growth assessment coupled with biosurfactant production were performed in triplicates under aerobic batch conditions in Erlenmeyer flasks containing mineral salts medium fortified with trace elements and crude oil as the sole carbon source.  Results: The generation times of strains NOB, EOB and ROB were 28.3, 44.9 and 46.8 h; with specific growth rates of 14.2, 8.9 and 8.6 h-1,respectively on crude oil. Gas chromatographic analysis of residual hydrocarbons from the growth cultures of isolates revealed that the three strains had degraded the oil by 89.34, 86.33 and 79.37%, respectively at the end of 21 day. The cell hydrophobicity of the bacterial strains also reached 83.6, 79.4 and 69.4%, respectively. Conclusion: The results from this study suggest that wood-digesting bacterial strains from a tropical lagoon possess hydrocarbon utilization capabilities. They could therefore have potentials for deployment in the bioremediation of petrochemical spills in polluted environments.
The Use of Fish Flour and Palm Sugar Medium to Grow Mataram Indigenous Isolate of Entomopathogenic Bacillus thuringiensis for Controlling Aedes agypti Larvae Bambang Fajar Suryadi; Hilal Aisyah Hidayat; Galuh Tresnani
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.04

Abstract

To grow Mataram indigenous local isolate of entomopathonic Bacillus thuringiensis using 4 combinations of fish flour and palm sugar natural liquid medium. The culture was then tested against 3rd instar Aedes aegypti larvae to gain lethal concentration (LC) value within 72-hour test. Four combinations of fish flour and palm sugar liquid medium (in 1:1; 1:3; 1:5 and 1:7 weight ratio) were made to grow B. thuringiensis isolate Bt-TP2B. Cell, endospore and protein crystal were recorded every 24 hours within 72-hour incubation. Seventy-two-hour-old B. thuringiensis cultures from those media were serially diluted, then tested against 3rd instar B. thuringiensis larvae. Larval death was recorded every 24 hours within 72-hour observation to obtain lethal concentration (LC) values. Results from all medium were compared to those of NYSM standar medium. B. thuringiensis isolate BT-TP2B could grow in fish flour and palm sugar natural medium. The highest toxicity (the lowest LC) was seen on B. thuringiensis grown on fish flour and palm sugar natural medium in 1:1 weight ratio. The LC50 values were 1.98x103 cell/mL (in 24-hour observation) and 1.49x103 cell/mL (in 48-hour and 72-hour observation), while LC90 values were 1.35x103 cell/mL in 24-hour observation and 1.58x103 cell/mL in 48 and 72-hour observations). These values were lower (more toxic) compared to those of NYSM standard medium. Fish flour and palm sugar natural medium could be used to grow entomopathogenic B. thuringiensis. Moreover, this natural medium could increase toxicity of B. thuringiensis against B. thuringiensis3rd instar larvae. 
Nutritional, Hypoglycemic, and Haematinic Potentiality of Edible Mushroom Pleurotus tuber-regium (Rumph. ex Fr.) Singer Sukumar Dandapat; Manoj Kumar; Rakesh Ranjan; Manoranjan Prasad Sinha
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Mycochemical composition, calorific value, the antioxidant activity of Pleurotus tuber- regium was analyzed and impact of P. tuber- regium extract on rat model especially glycemic, vitamins and blood parameters were explored to validate its medicinal importance. Mycochemical screening showed the presence of biochemicals such as phenols, flavonoids, proteins, carbohydrates etc. The extract showed good antioxidant activity (33.62% total antioxidant activity equivalent to 21.30 µg ascorbic acid). The extract showed dose -dependent hypoglycaemic activity by significant decreased average blood glucose level at high dose (100.62 ± 1.04mg/dL) compare to control (124.40 ± 1.45 mg/dL), haematinic activity by elevation of hemoglobin (14.75 ± 0.24 g/dL) at a high dose of extract compared to control (11.66 ± 0.21 g/dL). P. tuber-regium extract elevated vitamin B12 of rats at high dose of extract (449.60 ± 3.12 pg/mL) compared to control (420.00 ± 2.86pg/mL). P. tuber-regium extract showed a positive response to hypoglycemic, haematinic, and vitamin level of the body and posses high calorific value. Hence P. tuber-regium can be used as good fodder, medicinal and nutritional supplement.
Nutritional Value of Selected Edible Ficus Fruit in Kuantan Nadira Nazua; Rozilawati Shahari; Che Nurul Aini Che Amri; Nur Shuhada Tajudin; Mohd. Radzali Mispan
Journal of Tropical Life Science Vol. 10 No. 1 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/10.01.02

Abstract

Interest in the nutritional value content in food had been increasing as one of the ways to reduce the disease among the human being. Ficus is one of the fruit that contained high in major source of nutrients. Considering the important towards the mankind, this study was conducted to evaluate the nutritional content of Ficus fruit. There were six species and varieties of Ficus used in this study. The fresh sample was collected from selected area in Kuantan, Pahang. The major proximal components such as moisture content, crude fibre, crude lipid, total ash, crude protein, carbohydrate and energy was analysed using AOAC method. Based on this study, the moisture content ranges from 36. 33 % - 49.67 % in F. carica cv. BTM6; crude protein 2.3 % - 4.58 % in F. carica cv. TGF; crude lipid 0.01 % - 0.11 % in F. carica cv. BTM6; total ash 14.94 % - 21.22 % in F. carica cv. Alma and crude fiber 0.88 % - 3.36 % in F. carica cv. BTM6. The highest value of carbohydrate and energy content was found in F. hispida with 43.86 % and 175.44 kJ, respectively. According to our findings, consumption of Ficus fruit is recommended for commercialized in green industry based on its nutritional content that was crucial for human health.
Morphological Variation of Malayopython reticulatus (Schneider, 1801) from Several Population in Indonesia Luhur Septiadi; M Fathoni; Berry Fakhry Hanifa; Amir Hamidy
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Reticulated python (Malayopython reticulatus) is a widely distributed snake covering throughout Southeast Asia and almost all of Indonesia archipelago and divided into several subspecies based on morphological variation and its locality. Morphological variation data of M. reticulatus from Indonesia population has never been done thoroughly. This study aims to determine the morphological variations based on 21 meristic and 3 morphometric characters from several populations in Indonesia. The data was collected from the Museum Zoologicum Bogoriense (MZB) Cibinong, Indonesia and other additional collections that are carried out from June to July 2018 and then analyzed by Principal Component Analysis (PCA) to determine the population grouping. The result showed a high variation on the scale range of anterior prefrontal, posterior prefrontal, frontal, parietal, preocular, postocular, loreal, temporal, upper labial, lower labial, and dorsal scales. Ventral and subcaudal scales in male and female specimens show high variation in the total scale count, the ratio comparison of tl: SVL measurement, indicated the sexual dimorphism. Prefrontal (anterior-posterior) and frontal scales show high variation and difficult to distinguish between each locality at the subspecies level and suggest it to be intra-specific variation. There is no significant grouping were found between populations from data on morphological variations.
First record of the genus Metacirolana sp. and Cymodoce tribullis (Crustacea; Isopoda) from South Andaman, Andaman Islands, India Pathan Anil; Ameen Ummath; Bijin Mohammed PS; Kaderambil Arjunan Jayaraj
Journal of Tropical Life Science Vol. 10 No. 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.08

Abstract

Isopoda are one of the most poorly known crustaceans in Andaman and Nicobar Islands. The present study has recorded genus Metacirolana sp. and Cymodoce tribullis, for the first time from South Andaman. This recent sighting provides a new distribution of this two species in the Indian province. The specimens were collected from the seaweeds of intertidal regions of Kodiyaghat, South Andaman, Andaman Islands. The morphological characteristics of these two species were described. Furthermore, this study adds two more species to the isopoda check list of Andaman and Nicobar Islands. The present study also provides additional information on epifaunal communities associated with the seaweeds in the intertidal region of Kodiyaghat, South Andaman.
The Potency of Trichoderma sp. as A Biocontrol Agent against Fusarium sp. Pathogen of Porang (Amorphophallus muelleri Blume) Tuber Nindi Nazula Fajarini; Rodiyati Azrianingsih; Suharjono Suharjono
Journal of Tropical Life Science Vol. 10 No. 1 (2020)
Publisher : Journal of Tropical Life Science

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Abstract

Porang (Amorphophallus muelleri Blume) has high economic value in Asia. Porang tuber is high in glucomannan and thus becomes one of the exportable food commodities. Porang plants are susceptible to wilt disease caused by Fusarium sp. Trichoderma sp. has the ability to inhibit the growth of pathogenic fungi such as Fusarium sp. This research aimed to study the potency of Trichoderma sp. in controlling the growth of Fusarium sp. Trichoderma sp. was isolated from the rhizosphere soil in A. muelleri plantation, while Fusarium sp. was isolated from A. muelleri tuber infected by Fusarium. The fungi isolates were identified phylogenetically based on the similarity of Internal Transcribed Spacers (ITS) sequence. Both fungi were antagonistically assessed based on the dual culture method. The antagonistic assay showed that the two isolates of Trichoderma had the potency to inhibit the growth of the two isolates of Fusarium. Trichoderma sp. 2 has higher antagonistic potency than Trichoderma sp. 1. Based on ITS sequence similarity, Trichoderma sp. 1 and Trichoderma sp. 2 were identified as Trichoderma longipale and Trichoderma spirale respectively, while both pathogenic Fusarium were identified as Fusarium oxysporum. Trichoderma spirale therefore could be developed as a biopesticide agent in controlling Fusarium oxysporum.

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