Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Jurnal Kimia (Journal of Chemistry)

jchem
Website

Published by Universitas Udayana

ISSN : 19079850 EISSN : 25992740 DOI : 10.24843/JCHEM

Core Subject : Science,

Chemistry

Jurnal Kimia (Journal of Chemistry) publishes papers on all aspects of fundamental and applied chemistry. The journal is naturally broad in scope, welcomes submissions from across a range of disciplines, and reports both theoretical and experimental studies.

Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)

Articles 21 Documents

1 2 3

Search results for , issue "Vol. 9, no. 1 Januari 2015" : 21 Documents clear

PERBANDINGAN KUALITAS DNA DENGAN MENGGUNAKAN METODE BOOM ORIGINAL DAN BOOM MODIFIKASI PADA ISOLAT Mycobacterium tuberculosis 151 Dewi Andayani Farmawati; I Nengah Wirajana; Sagung Chandra Yowani
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Tuberculosis (TB) is a disease caused by the Mycobacterium tuberculosis. Isolation DNA is a necessary step to obtain the bacterial chromosomal DNA used in the Polymerase Chain Reaction (PCR). Boom isolation method is an isolation method commonly used in isolation DNA of M. tuberculosis. In Bali, the isolation DNA of M. tuberculosis conducted at the Laboratory of Biomolecular FK UNUD uses boom modification method. This research aims to compare the quality of DNA produced by the Boom methods and Boom modification methods. This research was started with the isolation process using Boom method and Boom modification and subsequently amplified by PCR. Detection of PCR products was performed with electrophoresis method. DNA quality was determined by the thickness of bands DNA PCR product and purity analysis by spectrophotometry UV-Vis. The results obtained show that the quality of DNA Mycobacterium tuberculosis 151 isolate using Boom modification method (Laboratory of Biomolecular FK UNUD) is relatively better than Boom original methods (Boom et al, 1990).

BIOAVAILABILITAS DAN SPESIASI LOGAM BERAT Pb DAN Cd PADA TANAH PERTANIAN BASAH DAN KERING DI DAERAH DENPASAR I Made Siaka; Emmy Sahara; Gusti Agung Putu Merta Dharmayoga
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

A research on the bioavailability and speciation of Pb and Cd in wet and dry soils in some agricultural areas in Denpasar has been carried out. Samples were taken from agricultural land in Peguyangan and Kesiman areas. This study was aimed to determine the total metal concentrations and the fractions of the species of Pb and Cd, as well as the level of the metal bioavailabilities. The study included the determination of the total Pb and Cd concentrations in the samples and extraction of the metal through the application of the sequential extraction technique followed by measurement of the metal with the Atomic Absorption Spectrophotometer. The result showed that generally the concentrations of Pb and Cd total in the wet soil from the two sampling areas were higer than those of the dry soil which were varied between 14,0484-16,1072 mg/kg and 1,5983-1,9885 mg/kg respectily.the results for Pb speciation in wet and dry soils from both locations as follows : the bioavailable fraction (EFLE fraction) and potentially bioavailable (consisting of Fe/Mn oxides and organic-sulfide fractions) were of 29,81%-83,66% and 2,44%-13,96 %, respectily, while the resistant (non-bioavailable) fraction were of 28,55%-55,11%. The results for Cd speciation in wet and dry soils from both locations were as follows : the bioavailable an potentially bioavailable fractions were of 28,62%-67,87% and 7,63%-48,39%, repectily, while the resistant (non-bioavailable) fractions were of 10,68%-30,92%.

SKRINING ANTIKANKER MELALUI PENDEKATAN UJI TOKSISITAS TERHADAP LARVA UDANG (Artemia salina Leach) SERTA IDENTIFIKASI GOLONGAN SENYAWA AKTIF PADA BUAH PLUM (Prunus domestica L.) Ni Made Susita Pratiwi; I Made Dira Swantara; Ni Luh Rustini
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolation and identification of toxic compounds from ethanol extract of Prunus domestica L. have been conducted. Extraction of 1000 gr of plum fruit produced 128,75 g concentrated ethanol extract. The results of toxitcity tests of the extract using Artemia salina L. larvae showed the LC50 value of 100 ppm. Partition of ethanol extract yielded chloroform (10,52 g), n-hexane (26,58 g), and water (52,57 g) extracts. The toxicity test showed the chloroform extract to be the most toxic with LC50 of 31,97 ppm. Chloroform extract was separated by column chromatography using silica gel as stationary phase and chloroform : ethanol : water (5:4:1) as mobile phase giving three fractions. The toxicity test showed that fraction II was the most toxic with LC50 of 364,74 ppm. The phytochemical test result showed that fraction II (the active isolate) belonged to terpene groups. Analysis using UV-Vis spectrophotometer showed three peaks at 241, 409, and 668 nm, showing the possibility of the presence of funtional groups of C-H aliphatic, C-O alkoholic and C=O, respectively

AKTIVITAS ANTIINFLAMASI EKSTRAK ETER KULIT BATANG TENGGULUN (Protium javanicum Burm) TERHADAP EDEMA PADA TIKUS WISTAR YANG DIINDUKSI DENGAN KARAGENAN A. A. Tia Santika Dewi; Ni Made Puspawati; Putu Suarya
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Stem bark of Tenggulun (Protium javanicum, Burm) has been used traditionally by Balinese people as anti-inflammatory agents. This research aimed to analyze phytochemical contents and to evaluate anti-inflammatory activity of stem bark ether extract of Tenggulun. Phytochemical study was done qualitatively using phytochemical reagents. Anti-inflammatory activity was evaluated on edema rats induced by carrageenan with given extract at doses of 125, 250, and 500 mg/kg b.w. Diclophenac sodium was used as the positive control. Phytochemical study revealed that the stem bark ether extracts consisted of alkaloids, steroids, terpenoids, and phenolic compounds. Anti-inflammatory activity test results showed at a dose of 125 mg/kg b.w, the extract only inhibit inflammation by 34.54%, while a dose of 250 mg/kg b.w gave inhibition of inflammation by 94.34%, and a dose of 500 mg/kg b.w can inhibit inflammation by 96.11% during 360 minutes observation. Probit analysis gave ED50 value of 103.252 mg/kg B.W.

ANALISIS FENOL DALAM URIN PEKERJA SALAH SATU STASIUN PENGISIAN BAHAN BAKAR UMUM DI KOTA DENPASAR Abdul Rahim; Ni Made Suaniti; Wiwik Susanah Rita
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Phenol is a compound produced by the metabolism of benzene contained in the fuel. The purpose of this study was to determine the content of phenol in the urine of workers at a Public Gas Station in Denpasar. The method used in this analysis was gas chromatography – FID (GC-FID). Urine was taken 3 times with an interval of 7 days from 3 workers. Control was taken from a non-gas station worker. Standard solutions used were mixtures of benzene and phenol with concentration of 1.25, 2.50, 5, and 10 ppm. Phenol calibration curve equation was y = 2.94x ??+ 0.23 with a correlation coefficient (r) = 0.9997. The concentration of phenol of samples varied ranging from 0.51 ppm to 1.38 ppm, while the phenol content in gasoline alone was 0.66 ppm. Phenol levels in the urines were still under normal value which is 25 ppm, while the level of benzene exposure was still under the standard concentration of 10 ppm.

UJI TOKSISITAS EKSTRAK DAUN WARU (Hibiscus tiliaceus L.) TERHADAP LARVA Artemia salina Leach SERTA IDENTIFIKASI GOLONGAN SENYAWANYA Ni Luh Rustini; Komang Ariati; A. A. Indah Purna Dewi; I Made Dira Swantara
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The “Brine Shrimp Lethality Test” (BSLT) is a preliminary test used to monitor the bioactive compounds from natural products as anti-cancer. This research aimed to determine of toxic compounds in the leaf of waru (Hibiscus tiliaceus L.). Maceration of 900 g waru leaf powder with 7000 mL of ethanol (5x24 hours) yielded 53,19 g ethanol extract with toxicity tests to larvae of Artemia salina Leach (LC50) of 79.43. The ethanol extract was partitioned to yield 1,81 g of n-hexane (LC50 = 63,09 ppm), 1,52 g of chloroform (LC50 = 1000 ppm) and 50,08 g of aqueous extract (LC50 = 316 ppm). The most toxic, n-hexane extract, was then separated , purified and identified. n-hexane extract was separated by column chromatography with silica gel 60 as stationary phase and n-hexane-chloroform-ethanol (5:4:1) as mobile phase to yield 7 fractions. Fraction one (F1) with a single stain on thin-layer chromatography was the most toxic (LC50 = 398 ppm). Identification with phytochemical test, UV-Vis spectrophotometer and IR spectrophotometer, showed that the isolate contained sterol compounds.

PREPARASI KATALIS NIKEL-ARANG AKTIF UNTUK REAKSI HIDROGENASI ASAM LEMAK TIDAK JENUH DALAM MINYAK KELAPA Imam Rasidi; Anak Agung Bawa Putra; I Wayan Suarsa
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study aims to determine the optimum concentration of nickel nitrate in the formation of a nickel catalyst embedded on activated charcoal by impregnation method, the chemical and physical properties of nickel- coconut shell charcoal in partidular acidity and surface area, as well as the ability of nickel-charcoal catalyst activity in the hydrogenation of linoleic methyl ester in coconut oil. The study begins with a preparation of a mixture of activated charcoal with NaOH activation and preparation of nickel-activated charcoal catalyst with a wet impregnation method. At this stage FT-IR and UV-Vis were used to characterize the products. The resulting catalytic activity was tested with the hydrogenation reaction. Esterification was then performed and the result was determined with GC-MS. The optimum concentration of the catalyst was found to be 0,3M. The catalyst acidity was indicated by the presence of oxide groups including carboxyl, phenols, aldehydes and carbonyl groups, whereas the average surface area was about 21.8753 m2/g. The catalytic activity of nickel-activated carbon in the hydrogenation reaction of methyl ester was optimum at a pressure of 2 atmospheres.

IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK DAUN TREMBESI (Samanea saman (Jacq.) Merr) SEBAGAI PENGENDALI JAMUR Fusarium sp. PADA TANAMAN BUAH NAGA Putu Sariningsih; Wiwik Susanah Rita; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study aimed to examine antifungal activity of Trembesi (Samanea saman (Jacq.) Merr) leaves extract against Fusarium sp on dragon fruit and to identify types of flavonoid compounds present in the extracts. Isolation of the flavonoids was started by maceration followed by fractionation into n-hexane, chloroform, and ethyl acetate respectively. Separation was carried out by preparative layer chromatography while identification was done using Ultraviolet-Visible and Infrared spectrophotometer. Antifungal activity test showed that ethylacetate extract has mild activity in inhibiting the growth of Fusarium sp. (inhibition zone 6.75 mm). The antifungal activity testing of three isolates positive flavanoid (B4, B5, B6) showed at the concentration of 10 % they have not given activity yet. The infrared spectra of isolates (B4, B5, B6) were very similar, therefore they have the same functional groups (OH, C-OH, aromatic CH, aliphatic CH, C=O, C-O-C ether, and aromatic C=C). The UV-Vis spectra showed isolates B4 gave absorption at a wavelength of 336.00 nm (band I) and 268.40 nm (band II), isolates B5 at 269.20 nm (bands II), and 325.40 nm (band I), and isolates B6 at 475.40 nm (bandI) and 282.40 nm (band II). Further UV-Vis identification using shift reagents suggested that isolates B4 was tentatively identified as 3,7,8,4 ', 5' pentahydroxy flavonols, isolates B5 as 3,5,4 'trihydroxy flavones, and isolate B6 as 3,5,7,8,3', 4 'hexahidroxy anthocyanine.

PEMBUATAN KOMPOSIT ZnO-ARANG AKTIF SEBAGAI FOTOKATALIS UNTUK MENDEGRADASI ZAT WARNA METILEN BIRU Dewa Ayu Wismayanti; Ni Putu Diantariani; Sri Rahayu Santi
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Preparation of ZnO-activated carbon composite with a mass ratio of ZnO : activated charcoal of 5:2; 5:1 and 15:2 have been carried out using the sol-gel method The characterisation of ZnO-activated carbon composite was done by using X-Ray Diffraction (X-RD) and Fourier Transform Infra Red (FT-IR). The characterization using X-RD showed that the ZnO has an hexagonal crystal structure with crystal size of 23.13 nm; 23.38 nm and 40.25 nm, while FT-IR spectra showed that there were Zn-O strain, C-O aliphatic, C-H bending, C-C aliphatic and O-H bending vibrations. ZnO-activated carbon composite was applicated as photocatalyst to degradate methylene blue by irradiatting the compound with UV light for 5 hours. The results showed that the ZnO-activated carbon composite at the ratio of 5:1 at optimum pH of 11 gave the highest percentage of methylene blue degradation which was of 99,97±0,02 %.

EFEK BERBAGAI MINYAK PADA METABOLISME KOLESTEROL TERHADAP TIKUS WISTAR Ni Wayan Bogoriani; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study was conducted over 10 weeks using several types of oils containing unsaturated fatty acids, saturated fatty acids and trans fatty acids, namely coconut oil, bulk oil, used cooking oil and pig fat. Animal tests were used to determine the effects of various oils on the metabolism of cholesterol in wistar rats. The study design was a randomized post-test only control group design, conducted in mice. Twenty-five rats were divided into five groups: control, treatment 1, treatment 2, treatment 3 and treatment 4, each of 5 rats. The control group was given standard diet only, treatment- 1 standard food and coconut oil 2.5 ml / day, treatment-2 standard food and bulk oil 2.5 ml / day, treatment-3 standard food and used cooking oil 2.5 ml / day and the treatment-4 standard food and pig fat 2.5 ml / day. After 10 weeks of treatment their blood plasm samples were taken for examination for total cholesterol, LDL, HDL, triglycerides, VLDL cholesterol, and the ratio of total / HDL. The results showed an increase in total cholesterol, LDL, triglycerides, VLDL, the ratio of total cholesterol / HDL and a decrease in blood plasm HDL cholesterol significantly (p <0.05) in the treatment of waste cooking oil compared with the control and other treatments. Based on these results it can be concluded that the used cooking oil has the potential to cause oxidative stress and inflamation which is a risk factor for atherosclerosis formation.

Page 2 of 3 | Total Record : 21

1 2 3

Filter by Year

2015 2015

Filter By Issues

All Issue Vol. 18, No.2, Juli 2024 Vol. 18, No.1, Januari 2024 Vol. 17, No.2, Juli 2023 Vol. 17, No.1, Januari 2023 Vol. 16, No.2, Juli 2022 Vol. 16, No.1, Januari 2022 Vol. 15, No.2, Juli 2021 Vol. 15, No.1, Januari 2021 Vol. 14, No. 2 Juli 2020 Vol.14 No.1 Januari 2020 Vol.13 No.2 Juli 2019 Vol.13 No.1 Januari 2019 Vol.12 No.2 Juli 2018 Vol. 12 No.1 Januari 2018 Vol. 11. No.2 Juli 2017 Vol. 11. No. 1 Januari 2017 Vol. 10, No. 2 Juli 2016 Vol. 10, No. 1 Januari 2016 Vol. 9, No. 2 Juli 2015 Vol. 9, no. 1 Januari 2015 Vol. 8, No. 2 Juli 2014 Vol. 8, No. 1 Januari 2014 Vol. 7, No. 2 Juli 2013 Vol. 7, No. 1 Januari 2013 Vol. 6, No. 2 Juli 2012 Vol. 6, No. 1 Januari 2012 Vol. 5, No. 2 Juli 2011 Vol. 5, No. 1 Januari 2011 Vol. 4, No. 2 Juli 2010 Vol. 4, No. 1 Januari 2010 Vol. 3, No. 2 Juli 2009 Vol. 3, No. 1 Januari 2009 Vol. 2, No. 2 Juli 2008 Vol. 2, No. 1 Januari 2008 Vol. 1, No. 2 Juli 2007 Vol. 1, No. 1 Januari 2007 More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Ni Putu Diantariani

Contact Email
jurnalkimia@unud.ac.id

Phone
+628123640424

Journal Mail Official
jurnalkimia@unud.ac.id

Editorial Address
Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Kampus Bukit Jimbaran, Jimbaran, Bali, Indonesia

Location
Kota denpasar,

Bali

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ni Putu Diantariani

Contact Email jurnalkimia@unud.ac.id

Phone +628123640424

Journal Mail Official jurnalkimia@unud.ac.id

Editorial Address Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Kampus Bukit Jimbaran, Jimbaran, Bali, Indonesia

Location Kota denpasar, Bali INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ni Putu Diantariani

Contact Email
jurnalkimia@unud.ac.id

Phone
+628123640424

Journal Mail Official
jurnalkimia@unud.ac.id

Editorial Address
Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Kampus Bukit Jimbaran, Jimbaran, Bali, Indonesia

Location
Kota denpasar,

Bali

INDONESIA