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INDONESIA
International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 8 Documents
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Search results for , issue "Vol 2 No 2 (2015)" : 8 Documents clear
ISOLATION OF PROTEASE ENZYME FROM CHAYOTE FRUIT (Sechium edule (Jacq.) Sw.) WITH AMMONIUM SULFATE FRACTINATION METHOD Ketut Ratnayani; Lia Kusumaningrum
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Protease is an enzyme that is capable to hydrolyze (breakdown) protein molecules intosimpler compounds such as small peptides and amino acids. The aim of the research was toisolate protease enzyme from chayote (Sechium edule (Jacq.) Sw.) using fractinationammonium sulfate method and to find out the optimum saturation level of the ammoniumsulfate. P rotease activity examination of each fraction of ammonium sulfate was performedusing Anson method. Protein content assay was determined using Biuret method. The resultsshowed that crude extract protease of chayote had specific activity of 3,7338 x 10-3 U/mg. Theoptimal saturation levels of ammonium sulfate for protease chayote precipitation was 40-50%. At this saturation level, the highest enzyme spesific activity were 16,00 x 10-3 U/mg,with four times purifying of protease enzyme from the crude extract protease.
THE USE OF ADVANCED GENOMIC PLATFORMS TO ACCELERATE BREEDING PROGRAMS OF THE INDONESIAN AGENCY FOR AGRICULTURAL RESEARCH AND DEVELOPMENT (A Review) I Made Tasma
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The use of advanced genomic platforms such as next generation sequencing (NGS) and highthroughput SNP (HT-SNP) genotyping platform facilitates the use of PGR collection in amore comprehensive manner for a more efficient breeding program. Indonesia is recognizedas the second richest mega-biodiversity in the world. This includes the plant and animal GRof agricultural importance. Few excellent example PGR of Indonesian origin included rice,banana, and sweet potato. The available GR diversity richness must be manipulated forhuman kinds (e.g. breeding purposes) to develop superior crop and animal for food, feed,ornament, and industry. A genomic-based breeding program facilitates the manipulation ofthe wealth GR collection in a more comprehensive, effective, and efficient manner. Thispresentation describes the current status of NGS-based genome sequencing project as well asthe application of HT-SNP array technology in genomic and breeding projects of theIndonesian Agency for Agricultural Research and Development (IAARD). The main cropsand animal under study included soybean, maize, rice, cacao, chili pepper, potato, physic nut,oil palm, and cattle. The sequencing project was done using NGS HiSeq2000 and the SNPchip genotyping study was done using the Illumina iScan. Genome Browser (GB) of thegenomic data was developed containing millions of genomic variations (SNP, Indels, andSSR). The GB is an excellent breeding resource to support breeding program of the crop andanimal under study. The genome browser also contains phenotypic and SNP genotypic dataof specific targeted populations. The SNPs discovered in this study are marker resources forHD SNP chip development. The SNP chips are useful for HT-SNP genotyping projects, GRcharacterization, and gene tagging to identify superior genes and QTLs for characters ofinterest to accelerate national breeding program of the national priority crops and animalunder study.
UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.
ASSESSMENT OF LIFE QUALITY OF CERVICAL CANCER PATIENTS DELIVERED BLEOMYCIN ONCOVIN MITOCYN PLATINUM (BOMP) CHEMOTHERAPY REGIMENS IN SANGLAH DENPASAR Rini Noviyani; I Nyoman Gede Budiana; Putu Ayu Indrayathi
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Cervical cancer disease is one of cancers that attack many women in the world and ranks second afterbreast cancer. Symptoms of cervical cancer may cause degradation quality of patients life.Chemotherapy is one of treatments of cervical cancer patients. Chemotherapy provides therapeuticeffects, however, it can causes a decrease in life quality due to its side effects. BOMP is one ofregimens that can be used as one of the management of cervical cancer. Assessment of quality ofpatients life with cervical cancer are needed to see the changes in the quality of life in patients besidesknowing the functional status changes from time to time. Monitoring the effects of treatment, andcollecting the data on quality of life can be used as initial data for consideration in formulating theappropriate action for the patient, And also assist physicians in selecting an effective and welltolerated drug for patients.The study was conducted in February to June 2014 in Obstetrics Clinic andCempaka Timur Room General Hospital (RSUP) Sanglah in observational design with cross sectionalprospective. The sample selection is done by consecutive sampling. The methods used for datacollection using questionnaires EORTC QLQ with interview techniques before and afterchemotherapy in patients suffering cervical cancer of squamous cell stage IIB - IIIB who had BOMPchemotherapy regimens for 3 series in RSUP Sanglah. Research for quality of life is conducted ingeneral and the 15 domains that affect the quality of life of patients. In this study, 12 patients indicatedthe inclusion criteria. There were significant differences in the value of the quality of life of patientswith cervical cancer squamous cell stage IIB - IIIB before and after BOMP chemotherapy with 0.001p value. BOMP chemotherapy regimens can improved the quality of life of cervical cancer patientsincreasing from 44.833 ± 6.235 to 60.333 ± 9.168. In 15 ratings domain included decrease the qualityof life of pain domain, nausea domain, vomiting domain, decreased appetite domains, fatigue domain,physical function domain, functional role domain, social functioning domain, sleeplessness domain,constipation domain and domain of financial difficulties and on the other hand emotional domainincrease.
MULTIPLEX PCR FOR DETECTION OF CAPSULAR POLYSACCHARIDES TYPES OF Streptococcus pneumoniae CLINICAL ISOLATES IN BALI Ni Nengah Dwi Fatmawati; Ni Made Adi Tarini; I Putu Bayu Mayura
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Streptococcus pneumoniae is causative agent of non-invasive and Invasive Pneumococcal Diseases(IPD). One of the major virulence factors is capsular polysaccharides (CPS). The CPS is known as thepneumococcal vaccine component. Several types of S. pneumoniae CPS are dominant in Indonesiasuch as types 6, 23, 15, 33 and 12 in West Nusa Tenggara, type 7F in Jakarta, and types 6A/B dan15B/C in Central Java. No data is reported from Bali related to S.pneumoniae CPS typing. Therefore,the aim of this study was to determine CPS types of S. pneumoniae isolates in Clinical MicrobiologyLaboratory, Sanglah General Hospital, Denpasar, Bali by using Multiplex PCR. Twenty-one isolatesthat were isolated from blood (11/52.4%), sputum (5/23.8%), and other clinical specimens (5/23.8%)were included in this study. Identification of S. pneumoniae was based on optochin test and presenceof pneumolysin gene (ply). Uniplex PCR was conducted to determine capsular type of each isolates,and then continued with Multiplex PCR 1 and 2, which used in-house positive controls. All isolateswere positive for the presence of ply, confirming the isolates were S. pneumoniae. Moreover, thisstudy showed that type 19F was the predominant type (7 isolates (66.7%)); 2 isolates (9.5%) werepositive for each type 23F and also for type 6A/B; and, there was only 1 isolate (4.8%) for each type7F and 15B/C. Total of 8 isolates (38.1%) were found to be nontypeable isolates. Multiplex PCR wassuccessfully identified different types of CPS. Development of Multiplex PCR could help indiagnosing and identifying capsular type of S. pneumoniae simultaneously.
CD4 COUNT FROM CRYOPRESERVATION OF BUFFY COAT AND PBMC Rasmaya Niruri; Inna Narayani; Wayan Tunas Artama; Mantik Astawa; Ahmad Hamim Sadewa
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This study aimed to determine CD4 count from cryopreservation of Buffy coat (BC) and PeripheralBlood Mononuclear Cell (PBMC) with and without ficoll. Fifteen EDTA Blood sample (2 ml for eachtube) were drawn from one adult healthy subject. The samples were categorized into five group beforemeasuring the CD4 level (which were fresh whole blood [Group(G)-I], BC without ficoll [fresh <GII>and frozen <G-III>] , and PBMC resulted from BC and ficoll isolation [fresh <G-IV> andfrozen <G-V>]. Each group was replicated three times. Blood storage before preparation was less thanfour hours. Two months cryopreservation using liquid nitrogen (in 40% FBS, 10% DMSO, and RPMI)was conducted. The mean value of CD4 count (cell /mu1) were 522 (G-I), 1410 (G-II), 906 (G-III), 807(G-IV), and 733 (G-V). CD4 count, after 2 month preservation in liquid nitrogen, of the BC sample (G-III) was higher (906 cell /mu1) than PBMC (G-IV) sample (733 cell /mu1).
RESPONSE OF OFFERED FERMENTED PURPLE SWEET POTATO (Ipomoea batatas L) SKIN AS ANTIOXIDANT COMPOUND IN DIETS IMPROVED MEAT QUALITY OF BALI DUCK Tjokorda Gede Belawa Yadnya; Ida Bagus Gaga Partama; Anak Agung Ayu Sri Trisnadewi; Ni Made Suci Sukmawati
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This experiment was carried out to study the response of offered fermented purple sweetpotato(Ipomoea batatas L) skin as antioxidant compound in diets on quality meat quality ofbali duck. Five treatment diets were used in a completely randomized design (CRD) consistedof control diet A (diet without containing purple sweet potato skin), diet B containing 10%purple sweet potato skin, diet C containing 10% fermented purple sweet potato skin, diet Dcontaining 20% purple sweet potato skin and diet E containing 20% purple sweet potato skin.Each treatment consisred of four replications and each replication consisted of five ducks.Variable s observed in this study meat quality with obyective method consisted meat colour,water concentration, water holding capacty (WHC), pH, and cooking loss. Organolepticquality consisted colour, smell, taste, texture, and to receive of whole. Anthocyanin, proteinand crude fibre consumption. In general, the showed that offered fermented purple sweetpotato skin in diets were significantly (P<0,05) increased the meat colour, water holdingcapacity, and pH, but on water concentration was not significantly (P>0,05) and on cookingloss was deceased significantly P<0,05). also could be increased organoleptic meat qualitywere colour, smell, taste, texture, and to receive of whole(P<0,05). Offered fermented purplesweet potato skin in diets could be increased anthocyanin and crude protein consumption.However, crude fibre consumption did not affected compared with the treament in A diet. Itwas concluded that the fermented purple sweet potato (Ipomoea batatas L) in diets couldimprove meat quality of Bali duck.
EFFECT OF THE SOIL PROTECTION SHEET AND THE BAG MATERIAL ON THE SOIL MICROORGANISMS AND THE INDIGENOUS ARBUSCULAR MYCORRHIZAL FUNGI IN THE VOLCANIC DEVASTATED SITE IN MT. BATUR, BALI, INDONESIA Nobuyuki Kohno; Kayo Gouya; Shin-ichi Sekiyama; Motoyuki Suzuki; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

In the volcanic devastated site of Mt. Batur located in the north eastern part of Bali, Indonesia,the test construction with the soil protection sheet and the bag material for the prevention of soilerosion and the revegetation had been executed in December, 2012 and the effect has beenverified by now. In this study, the effect of the sheet on the soil microorganisms was investigatedand the effect of the indigenous arbuscular mycorrhizal fungi, which had been put into the bagmaterial with 3 kinds of woody plant seeds, on the vegetation was verified. As a result, after 2years and 8 months from the construction, it was showed that the soil microorganisms under thesheet without the plant increased 2 to 2.4 times more than those in the bare site without the plant.Furthermore, those soil microorganisms under the sheet with the plant increased 8.4 to 8.9 timesmore than those in the bare site without the plant. It is considered that the cutting fragments in thelength of about 5cm of the gramineous plant root existed near the construction site put into thebag material with the woody plant seeds contributed to the colonization of the arbuscularmycorrhizal fungi into the roots of seedlings from the seeds and the survival of the seedlings fromthe seeds as the inocula of the fungi.

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