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INDONESIA
Journal of Agricultural Science and Biotechnology
Published by Universitas Udayana
ISSN : 23020113     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Mempublikasikan hasil-hasil penelitian yang berbasis bioteknologi dalam pemanfaatannya dibidang pertanian, termasuk biological control, pascapanen, serta semua penelitian tentang pemanfaatan komponen biologi yang dikemas dalam teknologi dalam lingkup pertanian secara luas.
Arjuna Subject : -
Articles 29 Documents
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KERAGAMAN Liriomyza spp. (DIPTERA;AGROMYZIDAE) YANG BERASOSIASI DENGAN BERBAGAI JENIS TANAMAN SAYURAN DAN TUMBUHAN LIAR DI TIMOR LESTE Americo Alves Brito; I Wayan Supartha; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Field research was conducted in Distrit Ainaro, Aileu, Bobonaro, Dili and Ermera. Maintenance and identification of Liriomyza and parasitoids was conducted in laboratory of Plant Protection, Department of Plant Protection of Agriculture, Ministry of Agriculture and fisheries Timor Leste. The purpose of the study to determine the diversity and abundance of leaf miner populations of Liriomyza spp. and parasitoids on vegetable crops in Timor Leste. The results showed there were two Liriomyza species, associated with host plants in the lowlands to the highlands of Timor Leste. The Both species are Liriomyza sativae (Blanchard) and Liriomyza sp. (Blanchard) The highest population abundance of Liriomyza sativa on tomatoes, red beans, Cucurbita maxima dan Cucumis sativus. while the highest populations of Liriomyza sp. on petsay crop.
EFIKASI MINYAK ATSIRI TANAMAN CENGKEH (Syzygium aromaticum (L.) Meer. & Perry), PALA (Myristica fragrans Houtt), DAN JAHE (Zingiber officinale Rosc.) TERHADAP MORTALITAS ULAT BULU GEMPINIS DARI FAMILI LYMANTRIIDAE Made Mika Mega Astuthi; Ketut Sumiartha; I Wayan Susila; Gusti Ngurah Alit Susanta Wirya; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The analysis of efficacy of clove oil (Syzigium aromaticum), ginger oil (Zingiber officinale), and nutmeg oil (Myristica fragrans) to hairy caterpillar was conducted. The hairy caterpillar were reported to attack some plants in 2010 to 2011 in Indonesia .To control the caterpillar, recently, peoples used chemical insecticide, however the impact of chemicals insecticide is dangerous to human being, livestock, and environmental. Therefore to minimizing those problems, the control methods should be environmental-friendly and safe against human being. One of those methods is utilizing the botanical pesticide which is extracted from tropical plants. Therefore efficacy of essential oils was done in order to find out the method to control population of it with environmental friendly approach. The experiment result shown at the concentration 10%, all of the essential oils are effective to kill the caterpillar (90-100%). Therefore the examinations of low concentrations of essential oils were conducted (5, 2, and 1%). The result of 1% concentration of ginger oil, nutmeg oil and clove oil are 80 %, 76 % and 68 % respectively.
UJI EFEKTIVITAS RIZOBAKTERI SEBAGAIA GEN ANATGONIS Sclerotium rolfsii PENYEBAB PENYAKIT BUSUK BATANG PADA TANAMAN KACANG TANAH (Arachis hypogea L.) Ni Kadek Desy Andya Dewi; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Groundnut (Arachis hypogea L.) is one of important commodities in Indonesia, however the production of groundnut is still relatively low which ispartly because of the plant disease i.e. stem rot disease caused by Sclerotium rolfsii. Rhizobacteria is one of the solutions to be used as the biological controlagent against S. rolfsii. The aim of this study is to know the effectiveness of rhizobacteria to control stem rot disease caused by S. rolfsii. The results the studyshowed that among 130 isolates of rhizobacteria tested against S. rolfsii, 11 isolates showed inhibitory activity, and among them one isolate, BjTk11 fromexposed the highest inhibitory activity (96.09%) against S. rolfsii. On a green house experiment, treatment with formula of isolate BjTk 11 at doses of 5 g, 10 g,15 g and 20 g per polybag effectively controlled the pre emergence damping off (disease incidence = 0%) while the disease incidence on control was 23.5%. Nopost emergence damping off symptom was observed on groundnut treatment with formula of isolate BjTk11, while the disease incidence on control was 75%. The number of sclerotia in soil of control was 79/gram of soil which is obviously higher than those of treatments. The mode of action the rhizobacteria isolatesBjTk11 in inhibiting the growth of S. rolfsii is through antibiosis where the rhizobacteria produce the antifungal compound Butanedial, R - (-) - 1,2propanediol, 2,3-Butanediol, Isopropyl Alcohol, Acetic acid, anhydride with formic Acid , 2-bromohexane, 4,5-Diamino-6-hydroxypyrimidine, 4H-Pyran-4one, 3-hydroxy-2-methyl and Cyclotrisiloxane, hexamethyl-. The presence of these compounds probably closely related to the inhibitory activity against S. rolfsii.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN LEGUMINOSAE UNTUK MENGENDALIKAN JAMUR Sclerotium rolfsii PENYEBAB PENYAKIT REBAH KECAMBAH PADA TANAMAN KEDELAI Komang Adi Mahartha; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Damping off disease caused by Sclerotium rolfsii is an important disease of soybean, because soybean plant will die after attacked by pathogen. This pathogen can survive for a long time in the soil by sclerotia form. Some of rhizobacteria isolated from the rhizospheres of Leguminosae plants were known could control soil-borne pathogens. This study aims to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to suppress the growth of S. rolfsii. Total of 11 isolates of 112 isolates rhizobacteria that could be isolated from Leguminosae plants had antifungal activity against S. rolfsii. Inhibition test of 11 isolates of rhizobacteria against S. rolfsii showed that three isolates had the highest ability to inhibit the growth of S. rolfsii namely isolates KtD1, KtD6, and KtB3. This result suggested that three isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of damping off disease on soybean.
PENGENDALIAN PENYAKIT VIRUS PADA TANAMAN CABAI RAWIT (CAPSICUM FRUTESCENS L.) DENG¬AN MULSA PLASTIK HITAM DAN PERAK Dewa Nyoman Darmayasa; I Gede Rai Maya Temaja; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The research of virus diseases management in chili pepper plant (Capsicum frutescens L.) by utilizing black and silver plastic mulch, was conducted in order to find the capability of black and silver plastic mulch in controlling virus diseases in chili pepper plant and also to find the capability of black and silver mulch in increasing yield of chili pepper plant. This research used randomized block design with 3 treatments and 9 replications. The result of the research shows that utilization of silver plastic mulch had ability to control virus diseases indirectly. The yield of chili pepper has incrising up to 143% with silver plastic mulch treatments.
PEMANFAATAN RHIZOBAKTERI PELARUT FOSFAT DARI TANAMAN LEGUM UNTUK PENINGKATAN PERTUMBUHAN DAN HASIL TANAMAN KEDELAI Ni Komang Budiyani; G. N. Alit Susanta Wirya; I Made Sudana; I Gusti Ngurah Raka
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Soybean is one of the strategic commodities in Indonesia. Soybean became the third most important crop after rice and maize. Leguminose plants are known to have microbial diversity in soils through their root exudates and can have a positive impact on plant growth. The use of rhizobacteria, known as Plant Growth Promoting Rhizobacteria (PGPR), is a bacteria that lives in the root area (rhizospher) and plays an important role in the growth of plants with the ability to form colonies around the roots quickly and can maintain environmental sustainability, one of bacteria rootsis phosphate solubilizing bacteria. This research is conducted to determine the influence of phosphate solubilizing bacteria on the growth and yield of soybean crops. This study used a factorial pattern (RAK) with 3 repetition and 9 treatments. Treatments of Rhizo 09 isolates from undis Rhizosfer here showed the best results.The analysis of sequencing and pyhlogeni shows that Rhizo 09 has close proximity withFlavobakterium johnsoniae from China and having a high similarity (98%) and a small genetic distance (0.003).
STRATEGI PENGENDALIAN PAPAYA RINGSPOT VIRUS (PRSV) PENYEBAB PENYAKIT BERCAK BERCINCIN PADA PEPAYA I Gede Rai Maya Temaja; I Putu Sudiarta; Ni Nengah Darmiati; Ni Made Puspawati
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

ELISA test results of symptomatic leaves from Tabanan, Bali showed that papaya ringspot virus (PRSV) as causing agents of ring spot disease on papaya in Bali. The objective of the study were to determine the control strategies of ringspot disease on papaya in the field. Control strategies of ringspot disease tested in this study were the use of metallic gray plastic mulch and plant barrier. Research result showed that plant barrier can provide a buffer zone to protect papaya that are planted within the confines of the buffer.
UTILIZATION OF SDT-RT-PCR FOR PLANT VIRUS DETECTION I Gede Rai Maya Temaja; Ni Made Puspawati; Ni Nyoman Ari Mayadewi
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Metode simple-direct-tube (SDT) dikembangkan untuk preparasi     RNA virus atau viroid, yang selanjutnya dipakai untuk sintesis cDNA. SDT tidak memerlukan penyaringan atau sentrifugasi seperi pada ekstraksi RNA total menggunakan KIT. Metode ini juga tidak menggunakan antiserum dalam preparasi RNA virus. Di samping itu metode SDT dapat dikerjakan di laboratorium dengan temperatur ruang dan selesai dikerjakan lebih kurang dalam 25 menit. Penerapan metode ini dilanjutkan dengan reverse transcription-polymerase chain reaction (RT-PCR), dapat mendeteksi keberadaan tomato chlorosis virus (ToCV), tomato infectious chlorosis virus (TICV), chrysanthemum virus B (CVB), Potyvirus and chrysanthemum stunt viroid (CSVd). Hal ini menunjukkan bahwa metode SDT dapat dipakai untuk mendeteksi virus atau viroid.
Pemanfaatan Rizobakteri Penghasil Indole Acetic Acid Sebagai Agens Penginduksi Ketahanan Tanaman Tembakau Terhadap Cucumber Mosaic Virus (CMV) Ni Luh Gede Sriyanti; Khamdan Khalimi; I Gede Rai Maya Temaja
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Tobacco is a high economic value commodity, in order to meet the demand for tobacco in the market it requires good quantity and quality. One of the diseasethat often causes harm to tobacco is Cucumber Mosaic Virus (CMV). The utilization of indole acetic acid (IAA)-producing rhizobacteria can be used to increase plant growth and resistance from pathogenic infection. The aim of this study was to determine the effectiveness of IAA producing rhizobacteria formula in increasing the growth of tobacco plants in greenhouses, and to determine the effectiveness of rhizobacterial formulations in increasing tobacco plant resistanceagainst the infection of CMV. Identification of compounds in tobacco leaves that infected with CMV was carried out to determine the effectiveness of rhizobacteria in inducing plant resistance. Compound analysis was carried out using Gas Mass Chromatography Spectroscopy (GCMS). The effectiveness of rhizobacteria in increasing plant growth and resistance was conducted in a greenhouse. Based on the results of the study, four of IAA-producing rhizobacteria isolates were Stenotrophomonas maltophilia Sg3, Proteus mirabilis BjB17, Bacillus thuringiensis TNJbx3.3, and Bacillus cereus GR12, all the rhizobacterial isolates were effective in increasing plant height, leaf number, leaf chlorophyll content, wet weight and dry weight leaf. Based on the results of the rhizobacterial effectiveness test, all rhizobacterial isolates were effective in increasing plant resistance as indicated by a lower intensity of infection. Based on the analysis of the tobacco leaves compounds content showed that rhizobacteria S. maltophilia Sg3 and P. mirabilis BjB17 were able to induce resistance to tobacco plants by forming antiviral compounds namely 2-Naphthalene-sulfonic acid and rhizobacteria Bacillus cereus GR12 can stimulate the formation of 1-Naphthalene-sulfonic acid compounds and 2-Naphthalenesulfonic acidin tobacco as a form of induction of resistance to CMV infection.

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