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BERITA BIOLOGI

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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Search results for , issue "Vol 18, No 2 (2019)" : 26 Documents clear

KERAGAMAN MORFOLOGI Hoya purpureofusca Hook.f. ASAL TAMAN NASIONAL GUNUNG GEDE PANGRANGO Rahayu, Sri; Tyas, Kartika Ning; Wawangningrum, Hary
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Hoya purpureofusca Hook.f. (Apocynaceae: Asclepiadoideae) has been used as an ornamental plant and the international trade of this species has become increasing. This species has restricted distribution on the high elevation of Java and Bali mountains. This epiphyte climber has succulent leaves and umbellate delicate flowers. Flower has star shape, succulent and waxy, purple., c.1 cm in diameter. The observation on the morphological characteristic is aimed to select the best sample for ornamental plant. The total of 17 observed samples were obtained from three populations at Gunung Gede Pangrango National Park, West Java, Indonesia. The selection was based on the node length, size of the leaves, and flower number, size and color. The result showed that P01 from Cibodas could be developed as leaf ornamental plant, because its shortest node and small leaves. SP1 from Selabintana could be developed as flower ornamental plant, it has numerous, larger and deep purple flower. SP1 and P01 have the farest relatives distance among all observed accessions, but still have a similarity of 75%.

ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Susilaningsih, Dwi; Umoro, Asahedi; Ochieng, Fredrick Onyango; Widyaningrum, Dian Noverita; Susanti, Hani; Susilo, Hadi; Swastika, I Nengah; Widyastuti, Utut
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ?Carita? Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids.

NISHAH KELAMIN, HUBUNGAN PANJANG-BERAT DAN UKURAN REPRODUKSI HIU Hexanchus spp. DI PERAIRAN SELATAN NUSA TENGGARA Sentosa, Agus Arifin
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Hexanchus spp. are shark species that are often caught in the Indian Ocean southern Nusa Tenggara and its status is Near Threatened, but the information is still relatively limited. This study aimed to sex ratio, length-weight relationship and reproductive size of sixgill shark, Hexanchus spp. caught from southern Nusa Tenggara waters. This study had been carried out during 2016 at the Fishing Port of Tanjung Luar, East Lombok, West Nusa Tenggara. Shark data collection was done by measuring the length, weight, maturity and fishing data. Data were analyzed by formula of sex ratio, length-weight relationship, length at first maturity (Lm) and length at first catch (Lc). The results showed that Hexanchus spp. caught in the southern Nusa Tenggara waters consisted of H. nakamurai (81.82%) and H. griseus (18.18%). The sex ratio of both sharks was dominated by the female. The average of the total length of H. griseus (218.3 ± 96.08 cm). It was longer than H. nakamurai (127.6 ± 30.35 cm). The length-weight relationship of H. griseus was formulated as W = 0.0195 LT 2,6344 (R² = 0,9265) and H. nakamurai with the formula W = 0.002 LT 3,1098 (R² = 0.902). Both species have the size of Lm was bigger than the size of Lc which indicated to their population vulnerability.

ANCAMAN JENIS IKAN ASING LOUHAN TERHADAP IKAN ENDEMIK DI DANAU MATANO, SULAWESI SELATAN Haryani, Gadis Sri; Dina, Rahmi; Samir, Octavianto; Nasution, Syahroma Husni
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Lake Matano is one of the ancient lakes in Sulawesi where some endemic fish species of Telmatherina, Mugilogobius, Oryziasand Glossogobius matanensis lived. The presence of alien species can be on threat for the loss of biodiversity in fresh waters, including endemic fish in Lake Matano. The research aim to review the threat of louhan fish as alien species to endemic fish in Lake Matano. The study was conducted in Lake Matano at five stations in April and August 2015 namely Lawa, Nuha, Petea, Pantai Impian, and Pantai Kupu-kupu. Sam-ples were collected using experimental gillnet with mesh size of ¾; 1; 1¼; 1?2; 2; 2,5; and 3 inches. Fouteen species were found consist of 10 endemic species and red list.These were three of genus Glossogobius, seven species of Telmatherina, and four alien species of the carp (Cyprinus carpio), louhan, gourami (Trichopodus trichopterus), and tilapia (Oreochromis niloticus). The most common species of endemic fish is T. antoniae. Louhan is the result of a hybrid species Amphilopus citrinellus x Cichlasoma trimaculatum. The abundance of louhan is 64 in April and 40 fishes in August, respectively at a time caught. The percentage ratio of louhan to endemic fish was of 8.6% and 18.3%. This indicated that the louhan fish tend to be invasive and certainly threatened endemic fish survival in this lake. The maturity of gonads of louhan is in the level of I - IV that enables the fish to reproduce quickly.

BUDIDAYA UDANG GALAH (Macrobrachium rosenbergii (de Man, 1879)) SISTEM AKUAPONIK BERBASIS POLIKULTUR DENGAN IKAN TAMBAKAN (Helostoma temminckii Cuvier, 1829) Setijaningsih, Lies; Gunadi, Bambang; Supriyono, Eddy
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This experiment aimed to find out the optimum density combinations of prawn (M. rossenbergii (de Man, 1879) and fish of kissing gouramy (Helostoma temminckii Cuvier, 1829) in the polyculture based aquaponic fish culture system. There are four treatments and three replications applied in the Completely Randomized Design experiment. The treatments were the combination of giant freshwater prawn (U) and kissing gouramy (T) densities for one square meter (m2), namely (A) 30 prawns and 100 gouramy (U30T100); (B) 20 prawns and 50 gouramy (U20T50); (C) 30 prawns and 50 gouramy (U30T50) and (D) 20 prawns and 100 gouramy (U20T100). An equal density of aquatic plant of water spinach Ipomoea reptans Poir was planted in each treatment units. The results from 60 days experiment showed that the highest growth of prawn were achieved in treatment C i.e 9,355 ± 1,096 g and survival rates of 75.02±0.67%. The highest growth of kissing gouramy were achieved at treatment D i.e 13,688 ± 1,382 g and survival rates of 86.66±0.83%. The water spinach was able to reduce ammonia, nitrite and nitrate. The lowest reduction was achieved at treatment A, i.e., amonia 6.54 %, nitrite 1,33% and nitrate 5,90%. While the highest reduction of ammonia is 11.72% found at treatment D, 3,52% for nitrite at treatment C and 11,72% for nitrate at treatment D. The highest reduction of Total N dan Total P by water spinach were found at the treatment C i.e. 32.54% and D i.e. 47.62%. According to the dominance and abundance index of plankton, Chlorophyceae and Cyanophyceae were recognized as the dominant class in all treatments.

PERKEMBANGAN TEKNOLOGI SEL MAMALIA CHINESE HAMSTER OVARY (CHO) UNTUK PRODUKSI OBAT BERBASIS PROTEIN Santoso, Adi
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3705

Chinese hamsters ovary (CHO) and its derivative such as CHO-DXB11 cells, CHO-K1, CHO-DG44 and CHO-S are mammalian cells that are often used for production of therapeutic protein drugs. The CHO cells often used for protein production have several advantages including 1) host cells that are safe to use in drug production, 2) the level of production of proteins produced can be increased by amplifying genes using methotrexate (MTX), 3) having the capacity to make post-translation modificationsand 4) CHO cells can be adapted to grow in suspension. The high need for protein-based drugs triggers the development of basic knowledge and innovation in production of recombinant proteins. The impressive technological advances in CHO cell technology have made these cells can be used to produce proteins around 10 g/liter in order to meet the market demand. The first protein successfully produced using CHO mammalian cells was the therapeutic Tissue Plasminogen Activator (r-tPA, Activase) protein used for stroke patients. The presence of this drug is quickly followed by several other types of drugs. In this review, history of development of CHO cells, the contribution of CHO cells to basic research, progress of effective line cell screening and development technology are discussed.

KARAKTERISASI BIOMETRIK IKAN LELE DUMBO PERBANDINGAN DENGAN IKAN LELE AFRIKA (CLARIAS GARIEPINUS BURCHELL, 1822) Iswanto, Bambang; Imron, Imron; Suprapto, Rommy; Marnis, Huria
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3422

Lele Dumbo was used to be a superior clariid catfish ardely cultured in Indonesia. Despite its aquaculture success, there was uncertainty about its taxonomic identity, whether it belongs to an African catfish (Clarias gariepinus Burchell 1822) or a hybrid resulted from a hybridization between African catfish C. gariepinus and an Asian catfish C. fuscus. Though lele Dumbo was no longer popular, the genetic improvement program has successfully developed lele Sangkuriang strain, and that have recently been extensively cultivated in Indonesia. As a lele Dumbo strain, the identity of lele Sangkuriang is also uncertain, thus need to be verified. The present study aimed to investigate the similarity of lele Dumbo through morphometric and meristic characterizations using samples of lele Sangkuriang (collected from BBPBAT Sukabumi, BPBAT Cijengkol and PT STP) compared to those of African catfish C. gariepinus introduced from Thailand and Kenya. The characterizations were carried out through measurement of 20 standard morphometric characters and five meristic characters the data obtained were then analyzed using principal component analysis. The results suggested that the values of morphometric and meristic characters of all three samples of lele Sangkuriang were not different from those of African catfish C. gariepinus. Likewise, the results of principal component analysis performed on morphometric and meristic characters also revealed that morphometric and meristic characteristics of all three samples of lele Sangkuriang were not different from those of African catfish C. gariepinus. Those results revealed that biometric characteristic of both lele Dumbo and African catfish C. gariepinus was not different, thus they seem belong to the same species.

PENGARUH PERBEDAAN PADAT TEBAR LARVA TERHADAP PERTUMBUHAN DAN SINTASAN PADA IKAN UCENG (NEMACHEILUS FASCIATUS) Subagja, Jojo; Prakoso, Vitas Atmadi; Arifin, Otong Zenal; Kristanto, Anang Hari
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3561

Proper stocking density on larval rearing of barred loach (Nemacheilus fasciatus Valenciennes, 1846) is very important in terms of obtaining the best growth and survival rate. This study was aimed to observe the growth and survival rate of barred loach subjected to different larval stocking density. Fish larvae were obtained by artificial spawning from in aquarium. Larvae were stocked on aquariums (50 × 40 × 30 cm) with three stocking density treatments (5, 10, and 15 individuals/L) and three replications. The experimental fish larvae were fed by Artemia and commercial feed (crude protein content: 30%) and feeding frequency twice per day (ad libitum). The length and weight were reassured every 10 days during 70 days of rearing period. Temperature, pH, and dissolved oxygen were measured at each treatment. Data obtained from measurements were analysed to assess the length gain, weight gain, specific growth rate (SGR), biomass gain, and survival rate. The results showed length gain of 3.35 ± 0.01, 2.94 ± 0.10, and 2.82 ± 0.15 cm and weight gain of 0.573 ± 0.023, 0.436 ± 0.018, and 0.417 ± 0.043 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Specific growth rates and biomass growth were of 6.22 ± 0.41, 5.93 ± 0.21, and 5.68 ± 0.41 %/day and 94.04 ± 5.39, 146.80 ± 3.81, and 167.24 ± 17.48 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Meanwhile, the survival rates obtained in this study were 89.13 ± 7.25, 93.13 ± 5.13, and 76.50 ± 3.54 % in larval stocking density of 5, 10 and 15 individuals/L, respectively. Increased stocking density has a negative impact on the growth and survival rate of barred loach.

PERSPEKTIF GENDER SUKU OSING DI BANYUWANGI DALAM PENILAIAN KEMANFAATAN TANAMAN Prasetyo, Budi; Chikmawati, Tatik; Walujo, Eko Baroto; Zuhud, Ervizal A.M
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3689

The different roles and responsibilities of male and female of the Osing tribe influence the knowledge of the use of plants. The aims of research is to analyze the values of the benefits of plants based on gender perception. The research used a qualitative and quantitative methods. Data collection for the value of plant species utilization were carried out using Pebble Distribution Method (PDM) and analysis used Index of Cultural Significance (ICS). The Osing community recognised 86 plant species that were grouped in to eight beneficial groups. The male in three villages have a better understanding of ethnobotanical knowledge and practice than female on crops as staple foods, medicines, fruits, and firewood, while understanding of ornamental plants are relatively the same for both male and female. Female have a broader and more detailed understanding on vegetables, plant species used as building construction, and traditional rituals. The high PDM value in eight beneficial groups was supported by the high value of each ICS.

KERAGAMAN KERAPATAN KAYU BATANG DAN CABANG KOMUNITAS POHON DI HUTAN GUNUNG PAPANDAYAN, JAWA BARAT Tihurua, Eka Fatmawati; Sulistyawati, Endah
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3519

Wood density is the functional character which has important role in the function of ecosystem. Stem and branch wood density have different trait and effect on its ecosystem processes. The objectives of this research are to know the diversity of stem and branch wood density and to analyze whether branch wood density could be used to estimate stem wood density in the Mount Papandayan. Six plots of 0.1 ha at different sites (three plots each in interior and edge forest) were established. Branches were collected from trees with diameter at breast height larger than 10 cm, while stem wood density data were obtained from some wood density sources. Research results showed that stem wood density ranges were 0.35?0.82 g/cm3, while branch wood density ranges were 0.33?0.61 g/cm3. Average of branch wood density was lower (0.48 ± 0.09 g/cm3) than stem wood density (0.61 ± 0.1 g/cm3). Linear regression analysis indicated that branch wood density could be used to estimate stem wood density of trees in Mount Papandayan which is showed by R2 value and correlation coefficient of 0.28 and 0.55 (p value < 0.001) respectively.

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