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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 32 Documents
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Search results for , issue "Vol 5, No 1 (2000)" : 32 Documents clear
EFEKTIVITAS INOKULAN BRADYRHIZOBIUM DAN GALUR MUTAN KEDELAI TERHADAP KANDUNGAN N DAN HASIL DI LAHAN MASAM Gandanegara, S; Wemay, J; Idawati, Idawati; Ardjasa, Wayan Sabe
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.666 KB) | DOI: 10.14203/beritabiologi.v5i1.1101

Abstract

One field experiment had been conducted at Institute for Research and Agricultural Technology Evaluation at Taman Bogo FieldSta,to screen some Bradyhyzobium inoculants on soybean mutant lines developed for acid soil.Inoculants evaluated were single strain B-22,and its mixed inoculants (B-22+B37), (B-22 + G49), and (B-22+TAL 102) on two soybean mutant lines No.07 and 58,and cv.Wilis as check variety. Plant growth and N yield were determined at pod development (R4) and grain maturity (R8) stages.The effect of inoculation significantly influenced nodul-ation and N yield and tended to increase plant dry weight 25%.Mixed inoculant (B-22+B-37) showed good symbiotic compatibility with the three soybean genotypes tested,whereas inoculant(B- 22+G49) only showed good compatibility with mutant line No.58.Cultivar Wilis had broader symbiotic spectrum as compared to mutant line No. 07 and 58.The effect of plant genotypes,and its interaction with inoculants were significant on grain and N yield.It was observed that at pod development stage,the plant growth of mutant line No. 07 were slower than the other genotypes but it had better nutrient translocation rate to grain which resulted grain yield of cv. Wilis. The results showed that this mutant line could be developed for this area.
TIKUS PADA TIPE HABITAT YANG BERBEDA Maryanto, Ibnu; Kartono, Agus P; Sinaga, Martua H
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.671 KB) | DOI: 10.14203/beritabiologi.v5i1.1107

Abstract

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EFEKTIFITAS PEMBERIAN PROPOLIS LEBAH DAN ROYAL JELLY PADA ABSES YANG DISEBABKAN STAPHYLOCOCCUS AUREUS Dharmayanti, NLP I; Sulistyowati, E; Tejolaksono, MN; Prasetya, R
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.495 KB) | DOI: 10.14203/beritabiologi.v5i1.1097

Abstract

A study was done to evaluate the effect of bee propolis and royal jelly on Staphylococcus aureus growth and duration of abscess healing.In vitro and in vivo methods were used on the study. In vitro method conducted by dilution method. In vivo 3 study treatments were divided in four groups.Each group was infected by 1.66.10 cell/ml of S.aureus fo get abscess.The group consists of treatment by abscess surgical and given bee propolis (A),abscess surgical and given royal jelly(B),abscess surgical and not given medication,and group control, no abscess surgical nor given medication.In vitro result showed minimalbactericidal concentration value of bee propolis and royal jelly were the same at concentration 6.25%.In vivo result showed duration of abscess healing were significantly different (p < 0.01).
PENGARUH ARAH DAN UKURAN POTONGAN SISIK UMBI KERK LILY (Lilium longiflorum Thunb.) TERHADAP PEMBENTUKAN TUNAS MIKRO DAN BULBLET SECARA IN VITRO Priyono, Priyono; Winarsih, Sri
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.634 KB) | DOI: 10.14203/beritabiologi.v5i1.1102

Abstract

Mass production by using micro propagation technique has an important role for Lilium longiflorum regarding that it can produce seedlings in relatively short time, disease free, and regularly time. Several factors affected the successful micro shoots and bulblets formation, i.e.medium, explant, and temperature. This research aimed to know the effect of direction and width of scale section on the multiplication of kerk lily in vitro.Research has been conducted in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute, Jember.The research was arranged in factorial completely randomised design, i.e.direction of scale section and width of scale section. Two levels of direction of scale section namely longitudinal and transversal and five levels of width of scale section namely 1, 4, 6, 8, and 10 mm.The results showed that scales are able to produce micro shoot only, bulblet only, or both micro shoot and bulblet.The number of micro shoots produced from longitudinal section are higher than those of produced from transversal section. Width of scale section affected micro shoots and bulblets production, which their correlations are quadratic.The trend of micro shoots production was contrary with bulblet production.
PENDETEKSIAN BAKTERI Raistonia solanacearum, Yabuuchi et al 1995 MENGGUNAKAN TEKNIK REAKSI POLIMERASE BERANTAI DAN PEMBEDAAN STRAIN MENGGUNAKAN TEKNIK HIBRIDISASI DNA Suryadi, Yadi; Machmud, M; Suhendar, MA
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.994 KB) | DOI: 10.14203/beritabiologi.v5i1.1090

Abstract

Raistonia solanacearum,the bacterial wilt pathogen, has a wide host range and genetic variability.Rapid and sensitive molecular techniques need to be developed for eariy detection and strain differentiation of the pathogen.Molecular techniques such as PCR and DNA hybridization have been succesfully used to detect and identify bacterial plant pathogens including R.solanacearum.These techniques were adopted under Indonesian condition, using purified and crude DNA from infected plant samples.An R.solanacearum specific DNA primer (OH/Y2) was used in the PCR test,and a DNA probe 5a67 were used in the non-radioactive hybridization test.The PCR techniqe could be used to detect R.solanacearum from infected plant samples in less than 5 hours.The DNA hybridization technique was applicable to differentiate strains ofR.solanacearum into three groups based on their DNA profiles.
PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Sulistinah, Nunik; Sunarko, Bambang
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.47 KB) | DOI: 10.14203/beritabiologi.v5i1.1104

Abstract

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(j was 6 h 40 minutes, the specific growth rate (J) was 0,046 h the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.
EFEK HYPOGLIKEMIK EKSTRAK ALKOHOL HERBA MENIRAN (Phyllanthus niruri L.) PADA KELINCI PUTIH JANTAN Chairul Chairul; Yuliasri Jamal; Zuherti Zainul
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1103

Abstract

A study on the influences of alcohol extract of Meniran or Phyllanthus niruri L. (Euphorbiaceae) to blood glucose concentration in rabbits (Oxytolagus cuniculus) had been carried out using glucose tolerance method.The extract was administered orally at 10, 20, 30, 40 and 50 mg/kg BW.Prior to tfie extract administration, all rabbits were put on hyperglycemic condition by injecting glucose solution intravenally at dose of 0.5 g/kg BW.The blood glucose concentration was measured every one hour starting at 2 hours before and until 7 hours after administration. Antidiabetic drug,tolbutamid 250 mg/kgBW.was used as control.The results indicated that extract with dose at 30 mg/kg BW gave a hypoglycemic effect similar to control (Tolbutamid). The blood glucose decreased regularly as the extract dose being higher.Using statistical analyses showed significant differences among the groups, especially after the 2 and 3 hours of treatment (P<0.05).
INDUKSI KALUS DAN REGENERASI TANAMAN DARI KULTUR ANTERA PADI Fl HYBRID (ORYZA SATIVA L.) Ita Dwimahyani; Ishak Ishak; Sobrizal Sobrizal
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1094

Abstract

A haploid breeding program was initiated to develop doubled haploid blast tolerance rice breeding via anther culture.Blast tolerant variety (Laka) was crossed with Kencana Bali (sensitive to blast).Anthers from F1 were cultured on two kind of media (combination of N6 macro salt and MS micro salt) containing 1 mg/l NAA + 0,1 mg/l kinetin called medium-1 and another one containing 1 mg/l 2,4-D + 0,1 mg/l kinetin called medium-2.Anthers were treated with cold shock (4 °C)for 5 and 10 days before cultured.Results of experiment showed that F1 plants derived anthers were able to form call Number ofplantlets were produced during anthers culture consisted of 482 green plants and 50 albinos. Most of green plants did not produced root.Root growth of plantlets were induced with 1.5-4.5 mg/l IBA in MS medium.
Di Sela-Sela Laboratorium dan Plot Eksperimen "PRESSURE CHAMBER", ALAT UNTUK MENGUKUR STATUS DAN TATA AIR DALAM TUMBUHAN B. P. Naiola
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1108

Abstract

Peran air yang sangat mendasar dan penting dalam tubuh tumbuhan, telah mendorong penelitian-penelitian intensif dalam aspek status air,tata air atau hubungan air-tumbuhan (Plant-Water Relations).Sebagai konsekuensinya, teknik-teknik maupun peralatan ikut dikembangkan untuk mengisi kebutuhan penelitian.Pressure Chamber atau Pressure Bomb merupakan salah satu alat yang khas dan spesifik digunakan dalam studi termaksud di atas.Apa sebenarnya PC, teori dasar dan bagaimana teknik pendekatannya dibahas dalam makalah ini.
EFEKTIVITAS ALKOHOL DAN CAMPURANNYA DENGAN FORMALIN SEBAGAI BAHAN PENGAWET SPESIMEN BINATANG UNTUK UDANG Nandang Suharna; Rita Dwi Rahayu
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1099

Abstract

This study was aimed at studying the effectiveness of alcohol, formaldehyde and their mixture in inhibiting fungal growth.This study contained two step. The first step was fungal isolation from specimen.One isolate was then selected to be inoculated into alcoholat various concentration (15%, 30%, 50% and 70%) with or without formaldehyde mixture.The second was fungal inoculation intoalcohol at various concentration (15%, 30%, 50%, dan 70%) with or without formaldehyde.The effectiveness of these subtances as specimen preservatives for shrimp were studied after six months incubation time.From the fisrt step, only genus group Monascus was isolated from seven specimens(Mabuia multifasciata, Myotis muricola,Chironax melanocephalus,Naya naya sputratrix, Miniopterus schreibersi blepotis,unidentified shrimp, unidentified Coelenterata).From the second step, the best growth of isolate Monascus sp.MM which isolated from the fisrt step was achieved in 70% alcohol.This fungus was not able to grow in both formaldehyde and its mixture with alcohol (1:1, 1:2, 1:3 and 1:4). The results also showed that Monascus growth was observed in alcohol with not sterilized shrimps without inoculation with Monascus sp. MM. It is recommended to apply mixture alcohol 70% with formaldehyde 1% at ratio 4:1 as animal specimen preservative to inhibit fungal growth.

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