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BERITA BIOLOGI
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Articles 2,125 Documents
PENGARUH PEMBERIAN BUI PHASEOLUS LUNATUS DALAM RANSUM TERHADAP KONSUMSI PAKAN DAN PERTAMBAHAN BOBOT BADAN AYAM KAMPUNG DARJONO DARJONO; DEWI MALIA PRAWIRADILAGA
BERITA BIOLOGI Vol 3, No 7 (1987): (Supplement)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i7.1381

Abstract

DARJONO & DEWI MALIA PRAWIRADILAGA 1987. Effect of level of Phaseolus lunatus seeds in the native chicken ration on feed consumption, and body weight gain Suppl. Berita Biologi 3 :61 -65. The experiment was aimed to obtain a basic guide of the amount of P., lunatus in chicken ration during growing period. Observation using 25 cockerels and 25 pullers, was based on Completely Randomized Design. The treatment consisted of level of P. lunatus in the ration, namely RK (as control ration), RI, RII, RIII, and RIV, containing 0%, 5%, 10%, 15%, and 20% respectively. Data was analyzed using Analysis of Variant, and the difference was tested by Honestly Significant Different Test. The results showed that the level of P. lunatus up to 20% had no significant effect (P > 0,05), on feed consumption and body weight gain during growing period. The highest feed efficiency for the cockerels was RI (0,0593) followed by RK (0,0559), RIII (0,0506), RIV (0,0478), and RII (0,0350) respectively; whereas for the pullets was RII (0,0267) followed by RK (0,0262), RI (0,0225), RIII (0,0220), and RIV (0,0102) respectively.
PENGARUH PENAMBAHAN JUMLAH INOKULUM DAN DI-KALIUM HIDROGEN FOSFAT PADA FERMENTASI PRODUKSI DEKSTRAN Triantarti Triantarti; Hendro Santoso M
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1060

Abstract

Dextran production is conducted by fermentation by using Leuconostoc mesenteriodes wich produces dextransucrase enzyme.Sucrose is converted to dextran by dextransucrase. Sucrose is a main carbon source in dextran fermentation. Hence, sugar cane juice mainly contains sucrose is potential material for dextran fermentation. The effect of inoculum concentration added at the beginning of fermentation of di-potassium hydrogen phosphate concentration in the medium were studied. L.mesenteroides B-512F was used. The results showed that there were no effect on optimum growth and dextran production when the inoculum concentration added at 1% and 5%(w/v). The only difference was inoculum at 1% (w/v) delaying the growth and dextran formation in comparison to the addition of 5%(w/v) inoculum. The optimum growth and dextran production were affected by di-potassium hydrogen phosphate concentration in the medium (0,5, 1,0 and 1,5% w/v).The growth was highest at di-potassium hydrogen phosphate concentration 1,5 % w/v. On the otherhand, dextran production was lower compared to the other treatments.
BARKODING DNA BURUNG ELANG (FAMILI ACCIPITRIDAE) DI INDONESIA Moch Syamsul Arifin Zein
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i2.3108

Abstract

The cytochrome c oxidase subunit I (COI) gene is a reprensentative of all the protein-coding genes of the mitochondrial DNA genome that has been widely used as an animal species identification tool. In this study, 86 sequences of DNA barcodes of members of the family Accipitridae in Indonesia including Nisaetus bartelsi, Nisaetus cirrhatus, Haliaeetus leucogaster, Spilornis cheela, Haliastur indus, and 11 sequences from Genbank were examined. Each species was confirmed through the Basic Local Alignment Search Tool (BLAST). The construction of phylogeny trees based on COI gene sequences was performed by the Neighbors-joining method where the calculation of the genetic distance matrix with the Kimura 2-parameter model was implemented in pairwise distance calculation in the Mega version 6.05 programe. The results of the analysis showed that the divergence within species ranged from 0 to 0.3% (0.13 ± 0.12%), between species ranged from 1.6 to 18.5% (12.8 ± 3.73%), between genera ranged from 13 to 18.6%, and the average in the Accipitridae Family was 11.8%. Therefore, it could form clusters in each species cohesively and clearly separated between the taxa analyzed.
DIVERSITY OF THE PARASITOID WASPS OF THE EULOPHTD SUBFAMILY EULOPHINAE (INSECTA: HYMENOPTERA, EULOPHIDAE) OF JAVA, INDONESIA AND THEIR DISTRIBUTION Rosichon Ubaidillah
BERITA BIOLOGI Vol 8, No 4(a) (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i4(a).1540

Abstract

Diversity of the Parasitoid Wasps of the Eulophid Subfamily Eulophinae (Insecta: Hymenoptera, Eulophidae) of Java, Indonesia and their distribution is presented for the first time. Most of eulophines are ectoparasitoids that attack concealed hosts in protected situations, such as leafminers, woodborers and leaf rollers. The subfamily are frequently involved in biological control programs directed against dipteran and lepidopteran leaf-mining pests, and many eulophine genera have been considered economically important. The taxonomy and distribution of the species in Asia, especially in Java, are however still poorly studied despite the fact that the subfamily is an important group for sustainable agriculture. This study is based on the specimens newly collected from many localities in Java and Bali using sweep netting, Malaise trapping, yellow-pan trapping and rearing from their hosts. All the three tribes (Elasmini, Cirrospilini and Eulophini) of the subfamily Eulophinae are recognized in the islands.A single genus of Elamini, three genera of Cirrospilini and 19 genera of Eulophini are recognized in the islands and they included 14 genera as new records for the islands and 66 undescribed species. A total of 110 species are recognized in Java and Bali; of those about 86% are new records for the islands and about 60% are undescribed species. Considering the species so far known for their world distribution pattern, eulophine species occurring in Java are mainly Oriental elements, a few species are Australian and a very small number of species is endemic, while several species that could have been artificially introduced with their hosts are worldwide in their distribution. Based on the climatic and geological features, the species diversity and geographical distribution of the Eulophine in Java and neighboring island are discussed.
KARAKTERISASI DAN OPTIMASI MEDIA PRODUKSIAMILASE , DARI Aspergilus niger DAN Aspergilus clavatus Elidar Naiola
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1213

Abstract

Six fungi isolates belong to Aspergillus spp were isolated from various samples and their ability to produce amylase has been tested. It was founded that all isolate shown the amylolytic activities which shown the clear zone areas after pouring with iodium solution. Two isolates which name Aspergillus niger (ISO 482) dan A. clavatus (ISO 468) to be the most active compare to another. The amylase activity of two isolate was studied in media contain rice and rice brain as a carbohydrate sources. Based on lower cost and easy to reach rice brain (local waste agriculture product) were choose as the alternative media to produce enzyme amylase from Aspergillus niger (ISO 482). The activities 2 of enzyme obtain was 54,14 x 10  U/ml (one unit activity is define as micromoles of glucose produce per ml per minute). The optimiation was done at room temperature for 7 days. The result showed the activity of enzyme increase during the fermentation process, at the first 2 2 day activity was 18,77 x 10 U/ml and reached the maximum activity (91,64 x 10 U/ml) after 3 days. The optimum temperature for enzyme reaction was 40 - 50 °C, optimum pH was (pH 5.0 - 7.0) and enzyme was relatively stable under such conditions.
APLIKASI VAKSIN Streptococcus agalactiae UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA BUDIDAYA IKAN NILA (Oreochromis niloticus) Taukhid Taukhid; Angela Mariana Lusiastuti; Tuti Sumiati
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.662

Abstract

The research with the aim to know the effectivity (yield gap) of the application of Streptococcus agalactiae vaccine (pure whole cell) in prevention of streptococcosis on tilapia (Oreochromis niloticus) culture has been carried out. The isolate of S.agalactiae – N14G was used as a master seed on vaccine production. Priming vaccination was administered by immersion method, and booster vaccination was taken th place two months latter by oral method. Challenge test at the lethal dose (LD50) against active bacteria was done at 14 days post booster vaccination, and observation was taken place for 14 days post artificial infection. The results of the research showed that the highest survival rate and relative percent survival (RPS) was found in group treated with Streptovac vaccine (S. agalactiae and A. hydrophila combination) (65.58% and 35.36%) followed by S. agalactiae vaccine (52.08% and 10.01%). The lowest survival rate was found in control group (46.75%). The result of confirmation effectivity of the vaccines by challenge test in the laboratory showed that the highest survival rate and relative percent survival (RPS) was found in S. agalactiae vaccine (50.00% dan 37.50%) followed by Streptovac vaccine (40.00% and 25.00%), and the lowest survival rate was found in control group (20.00%). Vaccination is better than the non vaccinated.
EKSTRAK DAUN MINDI (Melia azedarach) SEBAGAI BIOINSEKTISIDA UNTUK PENGENDALIAN INFEKSI Chrysomya bezziana PADADOMBA Yulvian Sani
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i4.2015

Abstract

Parasitic dermatitis may cause economic loss for livestock industry if it is not approriately controlled. Among the preventive measures available presently, the use of plant-derived insecticides is regarding as an alternative approach to control the disease since it is environmental and animal health safe. The purpose of this study was to assess the effects of mindi (Melia azedarach Linn.) extract leaves for controling Chrysomya bezziana in vitro and in vivo.The study showed that the methanolic extract of M.azedarach leaves affected various stages of C. bezziana larvae.A topical application of 0.25% methanol extract in vaseline mixture killed and inhibited the growth of larvae and reduced weight gain of both LI and LI larvae.The average mortality rate in a treated group (26%) was higher than a control group (19.2%).Greater reduction of average weight gain was also seen in the treated group (0.2719 gr.) compared to the control group (0.4761 gr). The larvae apparently had smaller size and wrinkled shape of anatomical structure seeming that they were inappropriately grown. While the average mortality rate of L2 was found higher in the treated group (46.8%) than the control group (22.4%). The leaf-methanol extract had greater effect to L2 than LI as seen higher mortality rate in L2 (46.8%) than the LI (26%). In conclusion that the higher dose rate of methanol extract applied will lead to high mortality of the larvae. The low mortality rate may be due to low concentration (0.25%) of leaf extract applied and short period of time for bioassay.These findings seem very promising, suggesting that may possible to increase larvicidal effects by increasing the concentration and time of assessment.
FLUKTUASI KOMUNITAS FITOPLANKTON DI WADUK MALAHAYU, BREBES, JAWA TENGAH MULYADI MULYADI
BERITA BIOLOGI Vol 3, No 3 (1985)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i3.1349

Abstract

MULYADI.1985. Phytoplankton community of Malahayu reservoir,Brebes,Central Java. Berita Biologi 3 (3) : 91 - 94. This study was carried out from July to October 1982, and a number ol 23 genera of phytoplankton was recorded.The population of Chlorophyceae was found to be the highest.The highest density of the phytoplankton community was recorded in September while the lowest density in August. The distribution of ftiytoplanktons in five stations was found to be relatively equal.
MUNDU: Garciniaxanthochymus Hook.f. ATAU G. dulcis (Roxb.) Kurz. Nanda Utami; Rismita Sari
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i6.850

Abstract

"Mundu" is common name for one of the member of Clusiaceae family (manggis-manggisan).In scientific writing it is sometimes called Garcinia xanthochymus Hook.f. or G. dulcis (Roxb.)Kurz. To determine the correct name for these two kinds of plant a study was conducted to review its taxonomic status. Based on morphological data, anatomical and phylogenetic analysis it is showed that the two species is separated but there are closely related, and in according to International Code of Botanical Nomenclature Garcinia xanthochymus Hook.f is the correct name for "MUNDU"
IDENTIFIKASI GALUR Ralstonia solanacearum DENGAN HIBRIDASI SLOT BLOT DNA Y suryadi
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3447

Abstract

R. solanacearum (RS), the casual agent of bacterial wilt is one of the most destructive pathogen in the tropical and sub tropical areas that affected various economic crops. Since the pathogen is very diverse, it is necessary to identify the variation of RS isolates. The Polymerase Chain Reaction (PCR) was used in this study to amplify 16S rRNA gane. Two DNA primer pairs namely pr#a and pr#b were used as probe to identify thirtyfour strains of Rs respresenting different biovars isolated from various host. The DNA probes were labelled using digoxygenin and deteced by DNA slot blot hybridization. Resutl showed that the DNA probes colud hybridzed specifically with target DNA, hence distinguish variation of Rs isolates. This assay will be of futher used in the strain identification of Rs from wide range of host from various geographic distribution.

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