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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 151   152   153   154   155 
KAPASITAS PREDASI TUNGAU Macrocheles merdatius BERLESE (ACARI: MACROCHELIDAE), PADA TELUR LALAT RUMAH Musca domestica LINNAEUS, DI LABORATORIUM Sri Hartini; Singgih H. Sigit; S. Kadarsan; F.X. Koesharto
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1284

Abstract

The capacity of Macrocheles merdarius Berlese to destroy Musca domestica Unnaeus eggs were related to the mite's development stage. Predation abiality of adult femak M. merdarius on fy eggs was 1.8 egg, followed by deutonymphal stage as much as 1.3 eggs and no predation by the protonymphal stage at all.
TINGKAT KELANGSUNGAN HIDUP FRAGMEN KARANG Acroporaformosa YANG DITRANSPLANTASIKAN PADA MEDIA BUATAN YANG TERBUAT DARI PECAHAN KARANG (RUBBLE) Nur Fadli
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.782

Abstract

Coral reefs around the world continue to decline.Corals killed by natural or anthropogenic disturbances are often degraded into rubble. This rubble is dynamic, easily shifted by storms and currents which may forms "killing fields" for coral juveniles, hindering coral recovery. In order to rehabilitate the coral reef, artificial substrates are always used as artificial reefs both for coral transplantation and recruitment. Unfortunately, most artificial substrates are expensive and used material from outside of the ocean (for example concrete/cement base). In order to develop a new low-cost artificial substrate that can be replacing the concrete/cement-base as a media for coral transplantation and coral recruitment, the modified coral rubble were tested in Seribu Islands, Jakarta. Two different net (nylon and polyethylene) were used to form or to shape rubble into a compact shape, stable and strong substrate.The stability of the rubble and the complexity of the surface which is created by the net make this substrate suitable for coral transplantation and coral recruitment. In 6 m, the highest survival was recorded for coral fragments that were transplanted on cement-base (58%). The lowest survival was recorded on nylon + rubble (16%). While, in 10 m, the highest survival was found on cement-base (40%) and the lowest was on polyethylene + rubble (3%). The modified coral rubble is a potential method for coral transplantation and coral recruitment. However, this approach requires testing at additional sites to determine the replicability of the results.
POTENSI Rhodococcus pyridinovorans GLB5 SEBAGAI BIOKATALIS DALAM KONVERSI SENYAWA METHIL SIANIDA DAN PHENIL SIANIDA Nunik Sulistinah; Rini Riffiani; Bambang Sunarko
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i1.2856

Abstract

Nitrile and amide bioconversion have received increasing attention due to their ability to provide a range of commercially important chemicals. The experiment was conducted to investigate the potential of bacterial isolate GLB5 to convert methyl cyanide and phenyl cyanide. The samples were collected from various industrial waste. Selection of isolates to utilize  these substrates as a sole source of energy, carbon and nitrogen was conducted on 96 whell microtitter plates, based on the growth ability using INT (Iodo nitrotetrazolium chloride) reagent. Based on the growth  pattern, it showed that the bacterial isolate GLB5 grew well and it was capable of utilizing  methyl and phenyl cyanide compound as the sole source of carbon and nitrogen.  The isolate GLB5 was isolated from industrial waste of Batik factory in Cirebon, and  identified as Rhodococcus pyridinovorans. Bioconversion of methyl cyanide using whole cells of R. pyridinovorans GLB5 showed that ethanamide (C2H5NO) and ethanoic acid (C2H4O2) were detected. Formation of ethanamide and ethanoic acid as the product of bioconversion, indicated that the nitrile hydratase and amidase enzymes  involved in the bioconversion process. Phenyl carboxamide (C7H7NO) as the product of phenyl cyanide bioconversion was also detected,  although  in  low  concentration. In this study, R. pyridinovorans GLB5 was capable of completely converting 300 mM methyl cyanide to ±  140 mM ethanoic acid in relatively short times (<60 minutes).
PERTUMBUHAN BIAK JARINGAN DENDROBIUM SCHULLERI PADA TIGA MACAM MEDIA DJUNAEDI GANDAWIDJAJA; MARIA IMELDA
BERITA BIOLOGI Vol 2, No 8 (1984)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v2i8.1413

Abstract

Teknik biakjaringan telah banyak dimanfaatkan baik untuk tujuan perbanyakan tanaman rnaupun untuk tujuan lain. Penerapan teknik ini dalam bidang peranggrekan terutama diarahkan pada tujuan komersial. Pada umumnya anggrek mudah diperbanyak dengan teknik biak jaringan, tetapijenis jenis tertentu ataupun bagian-bagian tertentu dari tanaman anggrek kadang-kadang sulit ditumbuhkan dalam suatu medium buatan (Mitra 1971, Sagawa dan Shoji 1967, Stewart dan Button 1975).Pemilihan bahan biak dan medium sangat menen+ukan berhasil tidaknya suatu biak jaringan (Gamborg et at 1976).Dalam usaha merangsang pertumbuhanbiak, berbagai macam hormon ataupun bahan organik, misalnya air kelapa, telah sering digunakan. Percobaan ini dilakukan dalam rangka mencari medium yang cocok bagi pertumbuhan biak jaringan Dendrobium schulleri J.J.S. sebagai alternatif cara perbanyakan dan pelestariannya. Pemilihan D. schulleri sebagai bahan percobaan didasarkan atas pertimbangan potensinya yang tinggi sebagai tanaman hias penghasil bunga potong. Pertimbangar lainnya ialah bahwa jenis yang asli dari Indonesia ini sudah banyak terkuras dan terdesak dari habitat aslinya sehingga dikhawatirkan akan mengalami erosi populasi ataupun erosi genetik.
PANEN HUJAN DAN ALIRAN PERMUKAAN UNTUK PENINGKATAN PRODUKTIVITAS PERTANIAN LAHAN KERING, PENANGGULANGAN BANJIR DAN KEKERINGAN Gatot Irianto
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1096

Abstract

Rainfall and runoff harvesting study at the Kali Garang basin, Semarang under the cooperation program between Centre for Soil and Agroclimate,Agency of Agriculture Research and Development (CIRAD) France and Government of Central Jawa for improving soil productivity,controlling flood and drought are presented in this paper.The results show that through water harvesting it is possible to increase diversity, improving added value and decreasing agriculture risks of agriculture commodity. Moreover rehabilitation of degraded land and soil conservation practices can be promoted.
PREFERENSI PERTUMBUHAN BIBIT GEMBILI [Dioscorea esculenta (Lour.) Burkill] ASAL BAHAN TANAM DAN TEKNIK PENANAMAN BERBEDA Ning Wikan Utami; Peni Lestari; Albert Husein Wawo
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3417

Abstract

Lesser yam (Dioscorea esculenta (Lour.) Burkill) tubers have two important roles in conventional cultivation, as the food as well as the propagation material. The stem cuttings technique is reported can not produce tubers, while micropropagation is less efficient due to the need of large capital, experts, and specific tools. The other hand, farmers are less interested in cultivating this commodity. It has been reported that mini tuber sett can be used for propagation, but it has not been able to explain the growth preferences in each part of the tuber and its effect on yield. The research aims to provide information about the growth preference of lesser yam seedlings from different planting materials and planting techniques and their effects on yields. The research was carried out in Research Center for Biology, LIPI, Cibinong. The experiment was arranged based on split plots with tubers (apical and basal parts) as the main plot and planting position (vertical and horizontal) as subplots. Each treatment was replicated 3 times, with 5 samples for each replication. The results revealed that the apical part produced better shoots and roots than the basal section. The apical dominance of the lesser yam tuber was very high. It is herefore buds that form in the apical tended to grow faster. This section also produced tuber with a better character. With this information, farmers recommended to use planting materials from the apical part of the tuber with horizontal planting position.     
KOI HERPES VIRUS SEBAGAI PENYEBAB KEMATIAN MASSAL Cyprinus carpio koi DI INDONESIA [Koi Herpes Virus the Causative Agent of Sporadically Mortality of Cyprinus carpio koi in Indonesia] Sri Oetami Madyowati; Sumaryam Sumaryam; A Kusyairi; Hari Suprapto
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1878

Abstract

Virus was isolated from infected koi Cyprinus carpio koi with KHV and confirmed by polymerase chain reaction (PCR) and transmission electron microscope (TEM). Fish that were naturally living near koi pond such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma did not infected after the cohabitation test. Based on the results KHV was the agent responsible for mortality of million cultured koi in East Java Province Indonesia. Absolute mortality was occurred in koi between 48-72 h post infection, while other cyprinids family was not caused in mortality. Other fresh water fish such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma was not infected. Virus-like particles as found in gill ofinfected fish with KHV.
REGENERASI TANAMAN DARI JARINGAN KOTILEDON +RUAS BENIH KEDELAI, {Glycine max L., KULTIVAR WILIS) DENGAN RADIASI SINAR GAMMA Dameria Hutabarat; Rivaie Ratma
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1245

Abstract

Soybean Wilis cultivar was efficiently regenerated in vitro via somatic embryogenesis. Cotyledonary explants were excised from mature geminating seeds. Seeds were germinated on agar solution and on B5 medium enriched with 5 ppm BA, 0,25 pom IBA and 500 ppm casein hydrolyzate. Cotyledonary nodes from both germinating seeds were excised and cultured on B5 medium enriched with 5 ppm BA, 0,25 ppm IBA and 500 ppm casein hydrolyzate. Age of seedlings had a remarkable influence on shoot regeneration. Cotyledon from seeds germinated on agar solution with light gave better result in shoot regeneration compare with those germinated in darkness. The highest number of regenerants per explants (5 shoots) was produced by cotyledon from seeds germinated on 65 medium enriched with 5 PPM BA, 0,25 PPM IBA and 500 PPM casein hydrolyzate in darkness. The seeds of soybean were exposed to gamma-rays doses 10 Gy then germinated on S5 medium enriched with 5 PPM BA,0,25 PPM IBA and 500 ppmcase/n hydrolyzate did not improve the number of plant regeneration.Only 5-day-old seedlings from seeds were exposed to gamma-rays dose 30 Gy could improve the number of shoot regeneration, one of the cotyledonary node treated produced 21 regeneration shoots.
OPTIMASI FREKUENSI PEMBERIAN VITAMIN C PAD A PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPES VIRUS (KHV) PADA IKAN MAS (Cyprinus carpio Linn.) Taukhid Taukhid; Angela Mariana Lusiastuti; Kusumasari Suryadi; Rosidah Rosidah; Gunawan Setiadharma
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.749

Abstract

The research with objective to understand optimization frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) feeding to control the Koi Herpes Virus (KHV) disease infecting common carp has been done in Fish Disease Laboratory Fishes were reared in plastic container (80 litres), with density of 20 fish sized 10 gram in average. The treatments were: (A) daily application, (B) three daily application, (C) five daily application, and (D) without vitamin C as a control. Examined fishes were challenged to KHV infection after the 21 days rearing period by cohabitation method for 2 weeks. Observations been done on behaviour, clinical signs and mortality of fishes. The results showed that the highest survival rate was found on the application o vitamin C given every 3 days (50.0%); followed by every day (12.5%), every 5 days (7.5%), and the lowest was found on contro group (1.3%). Control techniques in the case of KHV carp populations through the provision of vitamin C immunostimulatory conducted regularly since well before the existence of KHV infection provides the best protective level.
PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 Enung Sri Mulyaningsih; Hajrial Aswidinnoor; Didy Sopandie; Pieter BF OuwerkerV; Inez Hortense Slamet Loedin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

Abstract

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.

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