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BERITA BIOLOGI
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Articles 2,125 Documents
KEANEKARAGAMAN FLORA CAGAR ALAM NUSABARONG, JEMBER - J AWA TIMUR Tukirin Partomihardjo; Ismail Ismail
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.805

Abstract

Flora of Nusa Barong Nature Reserve, Jember-East Java, was intensively surveyed in 2005. At least 357 specimens consist of 282 species belonging to 232 genera and 88 families have been collected during the survey. Those are includes four species of Pterydophytes and 278 species of Spermatophytes. Among them there were three protected species Corypha utan, Excoecaria agalloca and Protium javanicum. Based on the IUCN categorization status, there were five species under threatened condition, i.e.Agalia edulis (LR/nt), Casearia flavovirens (VU.Bl+2c), Cycas rumphii (NT decreasing), Intsia bijuga and Intsia palembanica,both under VU.Al.cd.The natural population of these last two species was drastically decreasing due to the international trade. Most of the species recognized as new records for the island since there was no complete flora record of the area. Further studies are needed to gain more complete biodiversity information of small island ecosystem.
KERAGAMAN AKTINOMISETES ASAL SERASAH, SEDIMEN, DAN TANAH PULAU ENGGANO, BENGKULU Ade Lia Putri; Arif Nurkanto
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2238

Abstract

A total of 344 isolates of actinomycetes were isolated from leaf litter and soil samples collected from four locations of Enggano Island, Bengkulu. eighty eight isolates were selected using morphological characteristics and identified using 16S rRNA gene sequence analysis. Three families (Micromonosporacea, Streptomycetaceae, and Streptosporangiaceae) and eight genera were found from these samples. Approximately 80% of these strains belonged to Streptomyces group and 20% of isolates were similar to rare actinomycetes. Based on homology search (BLAST) and phylogenetic tree analysis, isolates of rare actinomycetes (EgA15, EgA30, EgA85, EgA386, EgA236, EgA243, EgA335, EgA112, EgA41,EgA312, EgA314) were identified as Kitasatosporia paracochleata (100%), Kitasatospora azatica (99%), Kitasatospora griseola (99%), Sphaerisporangium album (99%), Actinoplanes nipponensis (99%), Pseudosporangium ferrugineum (99%), Nonomuraea rosela (99%), Nonomuraea guangzhouensis (98%), Micromonospora chaiyaphumensis (99%),Micromonospora chaiyaphumensis (99%), and Couchioplanes caeruleus (99%) respectively.
SAMBUTAN KETUA LIPI Taufik Abdullah
BERITA BIOLOGI Vol 5, No 5 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i5.1448

Abstract

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STUDI POPULASI IKAN KALOSO (Scleropages jardinii) DI RAWA POMO KECAMATAN CITAK MITAK, KABUPATEN MERAUKE, PAPUA Agus H Tjakrawidjaja; Haryono Haryono
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1119

Abstract

Study on the population of bony tongue/kaloso fish (Scleropages jardinii) of Papua was conducted in April 1998, located in Rawa Pomo, Kecamatan (District) of Citak Mitak, Kabupaten (Regency) Merauke, Papua.The aims were to know density,fecundity, 2 population growth and related aspects.The method used was catch per unit efforts, in 3 stations, within the area of 50.000 m width; sampling was done in each 30 minutes and fecundity was counted by eye (visual).The results showed that abundance index was 1.862 2 2ind./m ,population density 0.0043/m ,population growth 0.017 ind/m each year and fecundity range was 87-161 eggs. The most th abundance in 8 size ranged from 45.81 to 48.54 mm SL;first maturation attained at + 30 cm. We noted the fishes biodiversity in the location i.e. 21 species belongs to 18 genera and 17 families.
KARAKTERISASI GEN PENYANDI PEDIOSIN PAF-11 PADA Pediococcus acidilactici F-11 Tri Marwati; Nur Richana; Eni Harmayani; Endang S Rahayu
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.495

Abstract

Pediocin PaF-11 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus acidilactici F-11. The objectives of this research is to find out the location and the nucleotide sequence of gene, which is involved in the production of pediocin PaF-11. Results showed that the pediocin PaF-11 from the cured cell of P. acidilactici F-11 loss the activity, suggested that the pediocin PaF-11 gene was carried in the plasmid. Agarose gel electrophoresis of P. acidilactici F-11 plasmid DNA with marker λDNA/HindIII showed that pediocin PaF-11 gene was carried in 12 kb plasmid. Amplification pediocin PaF-11 gene from P. acidilactici F-11 showed that uncured P.acidilactici F-11 culture contain plasmid DNA, indicated by amplification of the papA gene (256 bp). Cured P. acidilactici F-11 culture, plasmid eliminated, indicted by no aplicon DNA detected. This result also suggested that pediocin PaF-11 gene in P. acidilactici F-11 was carried in plasmid. Nucleotide of pediocin PaF-11 encoding gene was sequenced The alignment of that nucleotide sequence showed that pediocin PaF-11 encoding gene have the same sequence with pediocin PA.1 encoding gene in P. acidilactici PAC1.0 and P. acidilactici K10 and pediocin AcH encoding gene in P. acidilactici LB 42-923 and P .parvulus ATO77, and pediocin CP2 in P. acidilactici MTCC 5101.
AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK IXORA CUMINGIANA Kartika Dyah Palupi; Praptiwi Praptiwi; Dewi Wulansari; Andria Agusta
BERITA BIOLOGI Vol 19, No 1 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i1.3777

Abstract

Plants from Ixora genus exhibit a variety of pharmacological activities including antioxidant, antibacterial, and antitumor activities. This plants may contain many interesting bioactive compounds, especially phenolics and terpenoids groups. Ixora cumingiana is one of the Ixora species whose pharmacological effect has not been explored comprehensively. This study aimed to evaluate the antibacterial and antioxidant activity of eight extracts from bark and leaf of I. cumingiana. The plant samples were successively extracted using n-hexane, dichloromethane, ethyl acetate and methanol. The antibacterial evaluation was carried out against Staphylococcus aureus and the antioxidant activity was evaluated using a radical scavenging assay against 1,1-diphenyl-2-picrylhydrazyl (DPPH). Initial activity screening was performed using thin layer chromatography-bioautography followed by the determination of minimum inhibitory concentration (MIC) and the determination of antioxidant activity index (AAI) using microdilution technique. The dichloromethane extract of the bark as well as the n-hexane and dichloromethane extract of the leaf of I. cumingiana exhibited moderate antibacterial effect with MIC value of 128, 128, and 256 µg/ml, respectively. The methanol extract from the bark displayed a strong antioxidant activity (AAI = 1.5±0.13) and possessed the highest total phenolic content (43±0.91 mg AGE/ g extract). These experimental results showed that I. cumingiana is potential to be developed as an antioxidant agent rather than as an antibacterial agent. 
KARAKTERISASI DAN STUDI STABILISASI a-AMILASE Bacillus licheniformis TVII.6 MENGGUNAKAN BAHAN ADITIF Puji Lestari; Nur Richana; Rosmimik Rosmimik
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1914

Abstract

The limited stability of enzyme during long-term storage attributes to its reduced function. In this study, a-amylase from Bacillus licheniformis TVII.6 were formulated with different kind of additives for storage stabilization and better performance. Simultaniously, some minerals and calcium ion were applied to elucidate the inhibition and activation effects to a-amylase. Crude enzyme which was prepared by aceton precipitation was used for this stability test. It demosntrated that 10% of mannitol in citrate phosphate buffer gave the highest residual activity after 3 months of storage (98.5%). Calcium ion influenced the thermal stability of a-amylase and it gave optimum activity at 5 mM CaCl , thus the stability increased from 76.0%/90 C/2 hours to 114.8%/90 C/22 hours in comparison without calcium ions. Calcium ions (5 mM CaCl ) on the stability of a-amylase at 4 C also produced the 2highest residual activity, which remained 100% during 48 hours of incubation. Chemical compounds like FeSO , Na CO and EDTA 4 2 3acted as inhibitors, while (NH ) SO , CuSO , CoSO , MgCl and K HPO did not inhibit activity of a-amylase. NaOH and MnCl 4 2 4 4 4 2 2 4 2 compounds at low concentrations (2 mM) did not inhibit the enzyme but at 10 mM became a-amylase inhibitors. This amylase stability information is very important as a consideration when applying and storing the enzyme, thereby reducing the degradation a-amylase activity.
STUDI AWAL KEBERADAAN JAMUR TANAH PERAKARAN TUMBUHAN DI KAWASAN STASIUN PENELITIAN KETAMBE, TAMAN NASIONAL GUNUNG LEUSER, ACEH TENGGARA Nandang Suharna
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1274

Abstract

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PARTENOKARPI: BUAH TANPABIJI - APA, MENGAPADAN BAGAIMANA L Agus Sukamto
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.773

Abstract

Fruit growth and development occurred in plant for producing the offspring. Pollination and fertilization will form embryo and seed, which produce and supply plant growth regulators (PGR) for fruit growing. The role of seed can be exchanged with supply of POR exogenously and produce seedless fruit. Seedless fruit could be produced from triploid plant by crossing between tetraploid x diploid plants, in vitro culture of endosperm/irradiated pollen and genetic engineering or treated diploid plant by using PGR, pollen stress, chemical agent/ antibiotic and environment/ plant control. The benefit of triploid plant is quicker growth and produce of seedless fruits directly, whereas diploid plant could produce seedless fruits after fruit induction with special treatment. The other advantages of seedless fruit on certain plants are increased yield, fruit size/ weight,carotene and total sugar contents, decreased harvest period, yield fluctuation and blossom-end rot. Seedless fruit is preferential of consumer and give a higher price than the seeded fruit. Recently, seedless fruits of tomato, grape, citrus, cucumber and watermelon have been produced in commercial industries
APLIKASI MEDIA TUMBUH DAN PERENDAMAN BUI PADA PERKECAMBAHAN JELUTUNG (Dyera costulata (Miq.) Hook, f) Ning Wikan Utami; EA Widjaja; Arief Hidayat
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i4.2119

Abstract

Jelutung {Dyera costulata) is a potential plant species that has good an economic prospect,_especially for timber and latex production. The aim of this research is to know the effect of seed soaking treatments and growth media variation on seed germination of jelutung. The experiment was carried out in the Treub Laboratory, Research Centre for Biology-LIPI, Bogor, using a Complete Randomized Design with two main factors in three replications. The first factor is growth media i.e. peat + saw dush (1:2), peat + cocopeat (1:2), peat + zeolit (1:1), peat + saw dush (1:1), soil + peat (1:1) and soil (control). The second factor is soaking of seeds in fresh water for 0, 6, 12 or 18 hours prior to sowing. The results showed that there was no interaction effect of growth media and seed soaking treatments. However, both factors had individual significant effects on the germination of jelutung. Therefore it is recommended the best media for germination are peat + cocopeat (1:2), peat + zeolit (1:1), (or) peat +saw dush (1:2). These media had pH between 4 - 4.7. Seed soaking prior to sowing is not required for the germination of jelutung seed. In fact, the longer the time of seed soaked, the lower the germination of the seed. The highest germination percentage was 74%,which was recorded in treatment combination of mixed media peat + zeolit (1:1) without or with soaking of the seed in water for6 hours.

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