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BERITA BIOLOGI

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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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ANALISA GENETIK PISANG HIBRID DIPLOID BERDASARKAN MARKA RAPD (Random Amplified Polymorphic DNA) [Genetic Analysis of Diploid Banana Hybrids Based on RAPD Markers] Martanti, Diyah; Poerba, Yuyu Suryasari; Herlina, Herlina
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Banana breeding by crosses is one way to get new cultivars with a better quality. This study aims to verify molecular characteristic of diploid hibrid bananas i.e MDRK, SNMT and MDMT using Random Amplified Polymorphic DNA (RAPD) markers. Ten RAPD primers have been selected to detect the differences in molecular pattern from all three diploid hybrid bananas. The results showed that the hybrid combination of MDRK, SNMT and MDMT produced bands that shared some similarities with each parents by 43.83%, 51.25% and 47.21% respectively as the number of bands from type I, III and IV markers. Type III marker is the important marker for identification of male parents. Type VII marker (non-parent bands) was detected from hybrid MDRK, SNMT and MDMT by 18 (20.22%), 6 (7.5%) and 11 (12.64%) respectively. Marker of type VII can be used to identified hybrid because they are present only in offspring. The highest polymorphic bands was obtained by SNMT (83.9%) and the lowest obtained was performed by MDMT (71.25%). From this result, RAPD markers can be used as a tool to detect the differences of molecular pattern of diploid hybrid bananas.

DAMPAK PENAMBANGAN GAMBUT TERHADAP BIODIVERSITAS IKAN DI KABUPATEN BENGKALIS, RIAU Haryono, Haryono; Tjakrawidjaja, Agus H
BERITA BIOLOGI Vol 5, No 3 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on peat mining impact on fish diversity was conducted at Perawang, Bengkalis Regency,Province of Riau between 1996 and 1997.The purpose of study is to discover the diversity and potential of fishes in relation with water quality due to peat mining activities.The results recorded 17 species belonging to 14 genus and 10 families in mining areas, while in the protected areas (with 3 Watersheds) recorded 4 species in Bukit Batu, 21 species in Siak Kecil and 17 species in Kampar.Cyprinidae is dominant family in mining areas, while Belontiidae in protected areas.The water quality ranges are DO between 0.49 and 4.60 ppm, CO2 between 2.31 and 26.36 ppm, pH between 3.80 and 4.80 and turbidity 1.40 and 4.20. The potential offish found are mainly consumption and ornamental fish.

ISOLASI DAN IDENTIFIKASI SENYAWA ANTIBAKTERI MINYAK ATSIRI DAUN KEMBANG BULAN {Tithonia diversifolia (Hemsley) A. Gray} Soetjipto, Hartati; Dewi, Lusiawati; Prayitno, Sentot Adi
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Isolation and identification of chemical compounds and antibacterial compounds of the essential oil of Kembang bulan or Japanese sunflower {Tithonia diversifolia (Hemsley) A. Gray} leaves showed that the essential oil contain 29 components. The main components were caryophyllene (27.76%), nerolidol (21.81%),caryophyllene oxide (7.06%), copaene (6.41%) and bicylogermacrene (4.90%). The oil was screened for their antibacterial activity through bioautography method. Two antibacterial spots which had Rf 0.49 and Rf 0.61were obtained. The spot with Rf 0.49 was a mixture of 21 compounds with main compounds were heneicosane, nonacosane and tetratetracontane, while the spot with Rf 0.61 consisted of 22 compounds, which the main compound was nerolidol.

EVALUASI KONDISI HUTAN BERDASARKAN KERAGAMAN KUMBANG SUNGUT PANJANG (COLEOPTERA, CERAMBYCIDAE) 1 DI KAWASAN GUNUNG SLAMET Noerdjito, Woro Anggraitoningsih
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Evaluation of forest conditions using longhorn beetles (Coleoptera: Cerambycidae) fauna have been carried out at 3 parts of Mt. Slamet which are, northern part through Guci, southern part through Kalipagu, Baturraden and easthern part through Bambangan. In northern parts, research activities were conducted at about 1200-2500m above sea level (m.asl.), in southern part, research activities was conducted at about 700-1000m m.asl and in eastern part research activities was conducted at about 1500-2400m asl.Out of the 37 species of longhorn beetles were collected during this research, dominated by Sybra fuscotriangularis (55 specimens). The longhorn beetles collected in southern part, indicated lowest individual number (36 spesimen) but indicating hightest species number. In the contrary, the highest number of spesimens collected but lowest species number (10 species) was collected in the eastern part, while in northern part, it was collected 86 individu (17 species).The species compositions and the number of individuals of longhorn beetles collected from each parts (northern, southern and easthern part) were different with specific groups. Longhorn beetles species collected from secondary forest was similar to the primary forest.Distribution pattern of longhorn beetles in Mt. Slamet indicated that some forest species were found at certain altitude such as Batocera spp and Gnoma thomsoni which only found at 700-1000m asl. The forest area which this altitute (700-1000m asl.) was located at the southern part of Mt Slamet (Kalipagu, Baturraden) where known as the main support of water reservoir for electric power and seven spout water (Pancuran Tujuh). Thus this area has to be conserved from illegal logging and human activities.

KONSERVASI KEANEKARAGAMAN HAYATI TAMAN NASIONAL GUNUNG HALIMUN BAGI PEMBANGUNAN DAERAH TERLANJUTKAN Alikodra, Hadi S
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

PENURUNAN KADAR TANIN DAN ASAM FITAT PADA TEPUNG SORGUM MELALUI FERMENTASI Rhizopus oligosporus, Lactobacillus plantarum dan Saccharomyces cerevisiae [Reduction of Tannin and Phytic Acid on Sorghum Flour by using Fermentation of Rhizopus oligosporus, Lactobacillus plantarum and Saccharomyces cerevisiae] Setiarto, Raden Haryo Bimo; Widhyastuti, Nunuk
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Problems in using sorghum flour as food material was the presence of tannin and phytic acid that can reduce nutritition quality of sorghum flour. This study aimed to analyze the influence of Rhizopus oligosporus, Lactobacillus plantarum and Saccharomyces cerevisiae fermentation on the reduction levels of tannin and phytic acid in sorghum flour. Production of sorghum flour was done by four treatments in triplo i.e control (without fermentation), liquid fermentation (with L. plantarum and S. cerevisiae), solid fermentation (with R. oligosporus), mixture of solid and liquid fermentation (with R. oligosporus, L . plantarum and S. cerevisiae). Analysis levels of tannin and phytic acid in sorghum flour fermentation was performed by using spectrophotometry technique. The results showed that the fermentation process was able to reduce levels of tannin from 29.13 to 33.69% and phytic acid levels from 13.36% to 44.65% on sorghum flour. The highest reduction levels of tannin and phytic acid was produced in mixture of solid and liquid fermentation 33.69% and 44.65% respectively. Reduction levels of tannin and phytic acid can be caused of tannase and phytase enzyme which produced by the microbes during the fermentation processes. Â Â

EVALUASI PERTUMBUHAN DAN PRODUKSI TANAMAN KOLEKSI PLASMA NUTFAH JARAK PAGAR {Jatropha curcas L.) Effendi, Dedi Soleh
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

For anticipating future need for physic nut (Jatropha curcas L.) variety with high yield, short growing period demand and tolerant to pest and plant disease, an exploration for collecting new accession to East Nusa Tenggara (NTT), Bali and Gorontalo had been conducted. Beside exploration it has also been developed some accession and improve population from KIJP Pakuwon, Muktiharjo and Asembagus.Population formed in plan furrows and designed in sizes of plots. Accession from the exploration and donor have been planted on August, 2008 in PT Bumimas Ekapersadas plantation in collaboration with Indonesian Center for Estate Crops Research and Development at Pasirranji, Bekasi, West Java. Physical data and collection plant identification result was targeted to obtain variety that has short period to bare fruit, high yield rate and tolerant to pest and plant disease.Based on the examination of morphological growth and development, it can be concluded that Jcur.0006 showed characterstic of short and development in growth and production, accession that has characteristic a short period to bloom and high yield rate with perfect growth is Jcur.0006, plant number 15, and Jcur.0030 with plant number 8, but this variety is less tolerant to louse pest.With assumption that each branch has one bunch, thus 1.600 dry seed weight equals to 1 kg and 2 m x 2 m planting range, first year production potential from each accession originated from Gorontalo Jcur.0006; while plant number 6 and from NTT Jcur.0030 plant number 8, may reach minimum rate not less than 2.25 ton/ha dry seeds and 2.39 ton/ha dry seeds.

KAJIAN KARAKTERISTK GENETIK RUSA TIMOR (Cervus timorensis timorensis Blanville, 1822) ( Study on Characteristic of Genetic of the Timor Deer (Cervus timorensis timorensis) Zein, M Syamsul Arifin; Surjobroto, Bambang; Solihin, Dedy D; Prijono, Siti N
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on characteristic of genetic of the Timor deer (Cervus timorensis timorensis)was conducted during the periode of June - November 1997.Twenty four blood samples from Timor, Semau, Pantar, and Alor Island,Nusa Tenggara Timur Province were examined for this study.Restriction site analysis of part of ribosomeRNA mitochondrial DNA (447 bp),using restriction endonucleasis Haelll and Mbol showed that there were two haplotype variation.

PEMANFAATAN TUMBUHAN PADA MASYARAKAT KASEPUHAN DESA CISUNGSANG DIKAWASAN TAMAN NASIONAL GUNUNG HALIMUN KABUPATEN LEBAK BANTEN Wardah, Wardah
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Kasepuhan ethnic group surrounding Mt. Halimun National Park uses plants traditionally. Based on our research in May 2004 in Cisungsang, Lebak District, Banten province, there are 113 species of plants belongs to 41 families used in many ways. There are 20 local rice varieties, 30 species as vegetables, 30 species as medicinal properties, 28 species as food, 2 species as ornamental plants, 2 species as handycraff and 1 species as for dye. The wisdom in the traditional knowledge of this ethnic group is be discussed.

SEBARAN DAN HABITAT KUKANG JAWA (Nycticebus javanicus) DI AREA PERKEBUNAN SAYUR GUNUNG PAPANDAYAN, KABUPATEN GARUT [Distribution and Habitat of Javan low loris (Nycticebus javanicus) in Vegetables Gardens at Mount Papandayan, Garut District Area] Wirdateti, Wirdateti
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1887

The need of medicine product are increasing now. The fermentation of rice by using Monascus purpureus produce lovastatin, pigments and citrinin as secondary metabolites. The aim of this study was to know the concentration of lovastatin, red and yellow pigments and citrinin in rice which fermented by Monascus purpureus that had been mutated by using ultraviolet irradiation.Three strains were used in this research, such as AsK, yellow Jmba and red Jmba.The result were analyzed by using HPLC and Spectrofotometer. The result showed that the yellow Jmba strain which treated by UV mutagenesis for 3 minutes contain higher lovastatin (1.798%) and pigment and lower citrinin (0.019%) compare to without mutagenesis. On the other hand ASK strain produced higher lovastatin (1.903%) but higher citrinin (1.270%).This research is expected to provide scientific information to the public. That M. purpureus contained lovastatin that is beneficial, red and yellow pigments for natural food colorant.

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