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BERITA BIOLOGI

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Published by Lembaga Ilmu Pengetahuan Indonesia

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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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BAKTERI ENTOMOPATOGEN SEBAGAI AGEN BIOKONTROL TERHADAP LARVA Spodoptera litura (F.) [Entomopathogenic Bacteria as Biocontrol Agent Against Spodoptera litura (F.) Larvae] Zulfiana, Deni; Krishanti, Ni Putu Ratna Ayu; Wikantyoso, Bramantyo; Zulfitri, Apriwi
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Spodoptera litura (F.) (Lepidoptera: Noctuidae) is one of the agricultural pests that attacking almost all kinds of herbaceous plants, especiallyvegetables. Insect control using entomopathogenic bacteria is an alternative strategy that is effective and has a lower environmentalÂ impact than the use of synthetic insecticides. The purpose of this research was to explore entomopathogenic bacteria that have insecticidalÂ activity against S. litura larvae at various stages of instars. The result showed that 25% of total number of isolated bacteria have potency asÂ entomopathogenic bacteria. Isolate Staphylococcus sciuri strain BLSP-3 and isolate Serratia sp. strain BLSP-4 showed the highest larvicidalÂ activity against the first and second instar larvae of S. litura 83% and 86%, respectively. The activity against on the third instar larvaeÂ however was only by 40%. However, the mortality caused by both isolates was lower than that of Bacillus thuringiensis (more than 90%Â mortality to the first and second instars and 80 % of the third instar larvae). It is suggested that both of isolates are potential to be developedÂ further as a biocontrol agent to control S. litura population.

RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements] Iskandariah, Iskandariah; Soelistyowati, Dinar Tri; Gustiano, Rudhy; Kusmini, Irin Iriana; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.

KARAKTERISTIK RAGAM KULTIVAR KELAPA (Cocos nucifera L.) YANG DIGUNAKAN SEBAGAI BAHAN UPAKARA PADUDUSAN ALIT DI BALI [Characteristic Variation of Coconut (Cocos nucifera L.) as Materials of Upakara Padudusan Alit Ceremonial in Bali] Kriswiyanti, Eniek
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Exploration have been conducted to determine characters variation of coconut (Cocos nucifera L.) usually used in Upakara Padudusan Alit ceremonial by Balinese people, covering coconut plantation areas in each regency (kabupaten) of Bali.The characterization is based on measurement and observation of parts of plant according to âDescriptors for Coconutâ (IBPGR, 1992). Upakara Padudusan Alit use five âcultivarsâ of coconut known as "nyuh panca warna", derived from coconut with specific individual character i.e. has bole. There are five cultivars C. nucifera L. var. typica Nar. ), i.e. âGading Tall, Bulan Tall, Udang Tall and Sudamala Tall. Besides there are 4 derived cultivars from Dwarf coconut (Cocos nucifera L. var. Riff nana), i.e. âGadang Dwarf, Gading Dwarf , Bulan Dwarf and âUdang Dwarf. Characteristics of each coconut are in certain part, like Gadang, Gading, Bulan, with green, yellow and white epicarp. The Nyuh Udang is red in calyx base or in mesocarp; inflorescence of âSudamala tallâ has two bracts, branched spatha and flat spikelet stalk. Determination key of these cultivars is produced.

REPRODUCTIVE BIOLOGY OF STRIPED SNAKEHEAD (Channa striata Bloch, 1973) IN BOGOR AND BEKASI, WEST JAVA [Biologi Reproduksi Ikan Gabus (Channa striata Bloch, 1973) di Bogor dan Bekasi, Jawa Barat] Saputra, Adang; Ath-thar, Muhamad Hunaina Fariduddin; Samsudin, Reza; Putri, Fera Permata; Prakoso, Vitas Atmadi
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Striped snakehead is one of the potential local species, however, its production in Indonesia still comes from capture fisheries. Over exploitation had decrease the population in nature. Domestication is one of promising tool to solve the problems faced. In order to breed the domesticated species, the information of their reproduction was needed. This study aimed to describe relationship between length and weight, length and fecundity, weight â fecundity and gonad weight â fecundity of the striped snakehead from West Java. A total of 19 mature female specimens were collected from Parung (Bogor), and seven mature female specimens were collected from Babelan (Bekasi). The results showed that the determinant value (R2), the first for Parung and the second for Babelan, between weight and fecundity were 0.599 and 0.843, length-fecundity were 0.541 and 0.841; gonad weight â fecundity 1.00 and 0.846 Gonadosomatic Index (GSI) ranged from 0.67 to 9.94% and 2.03 to 8.17%. Gonad Index ranged from 6.17 â 76.73 and 18.48 â 76.77, respectively. The data indicated that growth pattern was allometric. Length and body weight has linear relationship with gonads and fecundity. Fecundity has higher correlation to the weight rather than length. Gonad weight and fecundity has a strong relationship.

REHABILITASI LAHAN TERDEGRADASI PASCA PENAMBANGAN TIMAH DI DABO, PULAU SINGKEP: PERTUMBUHAN DAN PENAMPILAN PADI GOGO Harahap, Robinson; Siagian, MH
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1067

Exploitation of natural resources often brings unexpected effects on the enviroment. This is happened in well-known Indonesian mining area of tin i.e. in Singkep Island, Riau Province.The accumulation of tailing as a mining waste, degraded the soil quality both chemical and physical aspects.To restore the degraded (death) land post-mining, biological agents must be introduced, to restore the biological processes within the soil as close as possible to their original conditions pre-mining.Planting rice in post mining land is thought to be one of the step to biologically, restore the quality of post-mining soil. A study of the seven upland rice varieties was conducted at Dabo village, Singkep District, Riau, using Randomized Block Design (RBD), within the scheme of introducing biological agent to post-mining soil.The high varities of upland rice consisted Jatiluhur, Limboto, Maninjau, Ranau,TB154 E.TB-2, Way Rarem and as comparison was local Pacitan variety.The results indicated that the plant heigh appereance of the high varities of upland rice variety was better compared to the local Pacitan variety, but the amount of seedling per bush of the local Pacitan variety was more at 45 and 60 days after planting. The average of panicle length on the high varities of upland rice 2 was 73.34 cm which was longer then the comparing local Pacitan variety. Biomass weight and grains production per m on the high 2 varities of upland rice was heavier than comparing local Pacitan variety. The high production of grain per m were 520 and 510 grams on the TB154 E.TB-2 and Limboto varieties respectively.Among the high variety of upland rice there were high diversities 2on plant height, total seedling per bush, panicle length, biomass weight and grain production per m .There were assumed to be influenced by habitat and genetic factors.The high varities of upland rice were able to grow and produce well on the nutrient poor soil and at low soil pH (4.87).

KERAGAMAN GENETIK HlBRIDA BEBERAPA STRAIN IKAN NILA {Oreochromis niloticus Bleeker) Gustiano, Rudhy; Soelistyowati, Dinar; Fauzan, Agung Luthfi; Arifin, Otong Zenal
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Objectives of this study are to determine the genetic variability among progeny of intraspecific crossing between three strains of Oreochromis niloticus Bleeker (BEST, Nirwana, and Red NIFI) using Randomly Amplified Polymorphic DNA (RAPD) and truss morphometric. The results showed that hybridization enabled to increase genetic variability respectedly on progeny of Nirwana male crossed to BEST female (27,93%) as well as to Red NIFI female. An analysist of truss morphometric indicated that BEST population had the lowest similarity and largest genetic distance (0,6) to the Nirwana and Red NIFI which were closer to the hybrid populations.

KUALITAS KRIOPRESERVASI SEMEN RUSA BAWEAN {Axis kuhlii (Temminck, 1836)} HASIL PENANGKARAN [Quality of Cryopreserved Semen of Captive Bawean Deer {Axis kuhlii (Temminck, 1836)}] Wirdateti, Wirdateti; Nugraha, R Taufiq P; Semiadi, G; Widyastuti, SK; Yulianto, Yulianto
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Bawean Deer {Axis kuhlii (Temminck, 1836)} is an endemic deer species from Bawean island, Indonesia. Under the National Protection act, the species is listed as protected and in the IUCN is categorized as Critically Endangered. In order to maintain the existence of these species, wildlife conservation of germplasm is needed through development of technique of sperm cryopreservation for Bawean deer semen. The sperm were collected from captive animals in Station for Beef Cattle Research Center, Ministry of Agriculture, at Grati, Pasuruan, which consisted of two adult males and two sub-adult males. The research objective was to determine the quality and quantity of preserved sperm from captive deer Bawean in the form of frozen semen for conservation needs and further usage. Prior to sperm collections, the animal was anesthetized with mixture of xylazine and ketamin. Sperm was then collected by an electroejaculator using a 2-cm probe diameter and 17 cm of length. Extender solution used was tris glycerol. The semen was able to be collected only from two adult stags with sperm motility of 50-60% and sperm concentration was in the range of 500-1140 x 106sel/ml. This study suggested the need for monitoring the developmentof sperm in relation to antler development.

RESPON GALUR/VARIETAS KAPAS (Gossypium hirsutum L.) TERHADAP PUPUK DOSIS N dan ZAT PENGATUR TUMBUH PADA SISTEM TUMPANGSARI DENGAN JAGUNG [Responses of Cotton Lines/Variety (Gossypium hirsutum L.) to Dosage of Nitrogen Fertiliser and Plant Growth Regulator Under Intercropping with Maize] Kadarwati, Fitriningdyah Tri; Riajaya, Prima Diarini
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Cotton lines/varieties with high productivity require high availability of nutrients in the soil, especially nitrogen (N). To maximize the utilization and distribution of nutrients in the crops, plant growth regulator (PGR) is needed to optimize the cotton production. The research was aimed to estimate the suitable dose of N fertilizer and plant growth regulator suitable for the new cotton lines. The experiment was conducted in Mojomulyo village, Tambakromo District, Pati, Central Java, from May to October 2011. Â The research was arranged in a Split Plot Design with three replications. The main plots were three lines/varieties of cotton: 99022/1; 99023/5 and Kanesia 13. The subplots were sixdose Â of combination of N fertilizer with PGR namely (1) N 90 + mepiquat chloride; (2) N 90 + pachlobutrasol; (3) N 90 Â without PGR; (4) N 120 + mepiquat chloride; (5) N 120 + pachlobutrasol; and (6) N 120 Â without PGR. The results showed that under drought conditions, the use of PGR pachlobutrasol was better than mepiquat chloride. Pachlobutrasol has a higher impact on cotton production with high N fertilization (120 N/ha) resulting in 701.26 kg cotton/ha. When mepiquat chloride was added cotton production reached 665.37 kg/ha and 604.81 kg/ ha with no PGR. Production of cotton lines 99023/5 was 721.65 kg/ha higher that of Kanesia 13 (577,50 kg/ha).

OPTIMASI FREKUENSI PEMBERIAN VITAMIN C PAD A PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPES VIRUS (KHV) PADA IKAN MAS (Cyprinus carpio Linn.) Taukhid, Taukhid; Lusiastuti, Angela Mariana; Suryadi, Kusumasari; Rosidah, Rosidah; Setiadharma, Gunawan
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The research with objective to understand optimization frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) feeding to control the Koi Herpes Virus (KHV) disease infecting common carp has been done in Fish Disease Laboratory Fishes were reared in plastic container (80 litres), with density of 20 fish sized 10 gram in average. The treatments were: (A) daily application, (B) three daily application, (C) five daily application, and (D) without vitamin C as a control. Examined fishes were challenged to KHV infection after the 21 days rearing period by cohabitation method for 2 weeks. Observations been done on behaviour, clinical signs and mortality of fishes. The results showed that the highest survival rate was found on the application o vitamin C given every 3 days (50.0%); followed by every day (12.5%), every 5 days (7.5%), and the lowest was found on contro group (1.3%). Control techniques in the case of KHV carp populations through the provision of vitamin C immunostimulatory conducted regularly since well before the existence of KHV infection provides the best protective level.

INTERAKSI ANTARA Trichoderma harzianum, Penicillium sp. DAN Pseudomonas sp. SERTA KAPASITAS ANTAGONISMENYA TERHADAP Phytophthora capsicilN VITRO Suharna, Nandang
BERITA BIOLOGI Vol 6, No 6 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A preliminary study has been done to know antagonism capacities of three isolates of Trichoderma harzianum, two isolates of Penicillium sp.and one isolate of Pseudomonas sp.against Phytophthora capsici in vitro and interaction among those six antagonists.The highest antagonism capacity possessed by Penicillium sp. KN1, respectively followed by Penicillium sp.KN2,Pseudomonas sp. GH1 and the three T. harzianum isolates. Except for those three T. harzianum isolates, the two Penicillium sp.isolates and Pseudomonas sp. GH1 isolate indicated anti fungal activity against this fungal pathogen.Based on microscopic observation, there was no mycoparasitism within three T.harzianum isolates against Ph. capsici.While interaction occurred among antagonist showed that Pseudomonas sp.GH1 was antagonistic against the other five antagonists.Growth inhibition by Penicillium sp. KN2 showed against this plant pathogen.Beside the need of further study in green house and field, this result appears the need of study to clarify and identify of the chemical subtance of anti fungal possessed by Penicillium sp. KN1 dan Penicillium sp. KN2.The result showed that the six microbes most potential for biological control agents against Ph. capsici.

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