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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 [Segregation of hpt Gene by PCR Analysis and its Expression in Transgenic Rice Population Overexpressing HD-Zip oshox6 Gene] Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; OuwerkerV, Pieter BF; Slamet Loedin, Inez Hortense
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.

KEONG HAMA Pomacea DI INDONESIA: KARAKTER MORFOLOGI DAN SEBARANNYA(MOLLUSCA, GASTROPODA: AMPULLARIIDAE) Isnaningsih, Nur Rohmatin; Marwoto, Ristiyanti M
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.761

The golden apple snail Pomacea is an invasive species not only in Indonesia but mostly in Southeast Asia. The snail caused serious damaged on more than thousands hectares of rice-fields. The study was based on the specimens deposited at the Museum Zoologicum Bogoriense (MZB)aimed to evaluate the occurrence of Pomacea and its distribution in Indonesia.Based on the shell characters there are four species recognized which are P. canaliculata, P.insularum, P. scalaris, P.paludosa that found from many places, and noted that P. canaliculata has wider distribution from Sumatra to Papua. The description of each species was presented at this paper as well as the map of the occurrence of Pomacea in Indonesia.

PEMANFAATAN TUMBUHAN DALAM KEHIDUPAN MASYARAKAT SUKU MUYU DIDESA SOA DAN SEKITARNYA, MERAUKE, PAPUA Susiarti, Siti; Rahayu, Rita D
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Papua possesses high biodiversity and several ethnics.Muyu community at Soa Village, Merauke still has a close relationship with their surroundings, as found in the usage of natural medicine, traditional food and ritual ceremony.A research was conducted at Soa Village and surroundings, Merauke, Papua by open-ended discussion and observation methods.There are 37 species used to cure 26 kinds of diseases as traditional medicines by Muyu Tribe community. Commonly, they use the plants still in wild position, gathered from village surrounding and parts of plant used namely bark, root and leaves.Many kinds of traditional food as staple food like sago (Metroxylon sagu).Wati (Piper methysticum) is used in ritual ceremony.

KERAGAMAN AKTINOMISETES ASAL SERASAH, SEDIMEN, DAN TANAH PULAU ENGGANO, BENGKULU [Deversity of Actinomycetes From Soil, Sediment, and Leaf Litter Samples of Enggano Island, Bengkulu] Putri, Ade Lia; Nurkanto, Arif
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A total of 344 isolates of actinomycetes were isolated from leaf litter and soil samples collected from four locations of Enggano Island, Bengkulu. eighty eight isolates were selected using morphological characteristics and identified using 16S rRNA gene sequence analysis. Three families (Micromonosporacea, Streptomycetaceae, and Streptosporangiaceae) and eight genera were found from these samples. Approximately 80% of these strains belonged to Streptomyces group and 20% of isolates were similar to rare actinomycetes. Based on homology search (BLAST) and phylogenetic tree analysis, isolates of rare actinomycetes (EgA15, EgA30, EgA85, EgA386, EgA236, EgA243, EgA335, EgA112, EgA41,EgA312, EgA314) were identified as Kitasatosporia paracochleata (100%), Kitasatospora azatica (99%), Kitasatospora griseola (99%), Sphaerisporangium album (99%), Actinoplanes nipponensis (99%), Pseudosporangium ferrugineum (99%), Nonomuraea rosela (99%), Nonomuraea guangzhouensis (98%), Micromonospora chaiyaphumensis (99%),Micromonospora chaiyaphumensis (99%), and Couchioplanes caeruleus (99%) respectively.

PENGGUNAAN PACLOBUTRAZOLDAN ABA DAL AM PERBANYAKAN X NENAS SIMADU MELALUI KULTUR IN VITRO Purnamaningsih, Ragapadmi; Mariska, Ika; Supriati, Yati
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pineapple (Ananas comosus L. Merr.), represents an important crop in Subang. Somaclonal variation is one of the problem to develop pineapple, especially Simadu variety. Probability to conduct Simadu progeny from the mother plant is very low (5%).Its caused by chimeric of the somatic cells that form meristem.In vitro culture is the alternative method to solve the problem by using the meristem cells from Simadu fruit as explant. Unfortunately, genetic diversity has been observed in many spesies during tissue culture.This phenomenon is usually termed somaclonal variation. Many studies on pineapple demonstrsted that some in vitro propagated materials differ from the source materials from which they are derived.To minimize genetic variability, the use of growth inhibitor such as paclobutazol and absisic acid hopefully would gave the important role in genetic stability. The aim of the research is to multiply Simadu pineapple by using tissue culture technic. In vitro shoot induce from crown of the Simadu fruit until get the sterile shoots. Combination of kinetin (0-5 ppm) with paclobutrazol ( 0-0.1 ppm) or ABA (0-1 ppm) was used in the multiplication stage. Result showed that there are no interaction between kinetin and paclobutrazol or ABA, but there is influence of the single factor. Kinetin increase leave number but decrease plant height and root number. Paclobutrazol increase shoot and leave number, but decrease plant height and root number. There is no influence of ABA to plant height, shoot and root number but decreased leaves number.

EFEK FERRI SITRAT TERHADAP KEMAMPUAN KHAMIR Candida tropicalis DALAM MEREDUKSI 3-(4,5-DIMETHYLTHIAZOL-2-YL)- 2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) [Effect of ferric citrate on 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) reduction in yeast Candida tropicalis] Julistiono, Heddy; Muthmainah, Resti Siti; Adam, Adam
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Effect of iron (ferric citrate) on 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) reduction in yeast Candida tropicalis was investigated. Reduction of MTT in yeast grown in YMB media containing 5 mM of ferric citrate decreased significantly compared to that of yeast grown containing 0, or 1.25 or 2.5 mM ferric citrate after 24 h incubation. However, there was no difference in cell density among cultures treated with 0 mM, 1.25 mM, and 5 mM ferric citrate. Ferric citrate of 5 mM caused smaller colony when cells were grown on YPDG media. Reduction of MTT in smaller colony cells was weaker than that of with normal size colony. An antioxidant, Epigallocatechin Gallate (EGCG) of 0.01 % could not reverse MTT reduction caused by 5 mM ferric citrate. Since enzymes responsible in MTT reduction are usually located in mitochondrion, the data suggested that, in the condition of 5 mM ferric citrate might cause mitochondrion disorder without killing the yeast cells. The data was in concordance with other studies on other yeast or human cells. However, this study does not show role of free radicals provoked by high level of iron concentration.

KEANEKARAGAMAN JENIS POHON DI KAWASAN CAGAR ALAM DUNGUS IWUL, JASINGA, BOGOR [Tree Biodiversity in Dungus Iwul Nature Reserve, Jasinga, Bogor] Polosakan, Ruddy; Alhamd, Laode
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Dungus Iwul Nature Reserve is one of the remaining conservation areas in Java and has an unique vegetation. Study on tree biodiversity was carried out in one hectare permanent plot. The results showed that there were 78 species of 35 families and 63 genera with 1219 trees per hectare. The species of Orania sylvicola was very dominating the region, with high distribution (Frequency = 100%) and the total basal area (basal area relative; BA = 50.96%). Species of O. sylvicola (Important Value; IV = 129.48%) highly covered the area, followed by Mischocarpus sundaicus (IV = 19.93%), Diospyros frutescens (IV = 9.67%), Ficus variegata (IV = 9.38%) and Knema laurina (IV = 8.51%). Family with largest number of species was Euphorbiaceae (11 species), followed by Lauraceae (7 species) and 4 species of Fabaceae, Meliaceae and Rubiaceae.

TEKNOLOGI PENGAIRAN DAN PENGOLAHAN TANAH PADA BUDIDAYA PADI SAWAH UNTUK MITIGASI GAS METANA (CH4) Naharia, Orbanus; Saeni, M Sri; Sabihan, Supiandi; Burhan, Harris
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Global warming that caused by green house effect is one phenomena where short wave sunlight radiation penetrates the atmosphere and changes to become long wave on earth surface, when reaching the earth surface, a part of the wave reflected to atmmosphere,however not the entire reflected wave will be released to outer space; greenhouse gases layer in the atmosphere will reflect part of the wave to the earth surface resulting in increasing surface temperature.According to data from National Communication in 1997, agriculture and husbandry sectors give large contribution for the increasing green house gases particularly CH4 produced from rice field cultivation.Due to the reason, research for "irrigation and soil cultivation technology on rice field for mitigation of methane (CH4) gas emission in dry season" has been conducted. The objectives are to analyze different effect of continuously flooded (5 cm), intermittent irrigation and saturated water condition (0 - 1 cm water level) on CH4 emission, to analyze effect of soil cultivation and zero tillage on CH4 emission and to analyze interaction between irrigation treatment and soil cultivation on rice field for CH4 emission.The result indicated that type of irrigation systems affect the CH4 emission on rice field cultivation. Intermitten irrigation system shows able to suppress CH4 emission 56.34%, while saturated water condition system is 54.61%. Type of soil cultivation also affects the CH4 emission. Soil preparation with zero tillage by touchdown reduced CH4 emission by 25.55%, while by gramoxone the reduction was 48.84%. The lowest CH4 emission was under saturated water condition system combined with zero tillage treatment. Combination saturated water condition system by touchdown and zero tillage treatment is resulting emission in amount of 61.54 kg/CH4/ha/season. The combination of saturated water condition system by gramoxone and zero tillage treatment is resulting emission in amount of 88.12 kg CH4/ha/season.Combination of all treatment has no significant difference on rice yield.

INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Sapsuha, Yusri; Soetrisno, Djoko; Kustantinah, Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33Â±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33Â±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33Â±11.50.

IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN env SU [Identification of Jembrana Disease Virus by Using a Molecular Marker of env SU Gene in Bali Cattle] Indriawati, Indriawati; Margawati, Endang Tri; Ridwan, Muhammad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of Â± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.

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