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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 206   207   208   209   210 
PERTUMBUHAN DAN PRODUKTIVITAS Oedogonium sp. PADA INTENSITAS CAHAYA YANG BERBEDA Niken Tunjung Murti Pratiwi; Qadar Hasani; Ahmad Muhtadi; Neri Kautsari
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3890

Abstract

Oedogonium sp. is important, not only from an academic standpoint but also has great ecological significance, especially in limnology. This study examines the growth and productivity of Oedogonium sp. under different light intensity in laboratory scale. The main research includes Oedogonium sp. culture, measurement of growth (doubling time (DT) and relative growth rate (RGR)), measurement of primary productivity, and the effect of light measurement on cells through observation of histological preparations. The results showed that Oedogonium sp. on indoor culture showed negative growth (RGR of -0.054), whereas in semi-outdoor culture showed positive growth (RGR 0.046 - 0.47 g / day and DT for 14.72 - 15.09 days. Primary productivity and respiration in 1000 lux lighting cultures showed greater value than cultures in 650 lux lighting. The length of Oedogonium sp. ranged from 18.68 - 34.47 µm in dark light, 51.33 - 95.00 µm in moderate light, and 48.83 - 70.75 µm in bright light. This study showed that in indoor culture, the availability of light, temperature, pH and DO significantly affect the growth of Oedogonium sp., whereas in semi-outdoor culture, temperature, pH and DO factors was not limiting factors. The difference of light intensity influenced the growth and productivity of Oedogonium sp.   
ASPEK BIOLOGIS IKAN EKOR PEDANG (Xiphophorus hellerii HECKEL, 1848) DI CATUR DANAU BALI I Nyoman Yoga Parawangsa; Prawira Atmaja Rintar Pandapotan Tampubolon; Nyoman Dati Pertami
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3864

Abstract

Ikan ekor pedang (Xiphophorus hellerii) merupakan jenis ikan asing yang mendiami catur danau Bali (Danau Tamblingan, Danau Buyan, Danau Bratan dan Danau Batur). Tujuan penelitian ini adalah untuk mengungkapkan nisbah kelamin, pola pertumbuhan dan kondisi ikan ekor pedang di catur danau Bali. Nisbah kelamin ikan ekor pedang di keempat danau berada dalam keadaan tidak seimbang. Proporsi ikan jantan di Danau Tamblingan, Danau Beratan dan Danau Batur lebih banyak daripada ikan betina dan kondisi sebaliknya ditemukan di Danau Buyan. Pola pertumbuhan ikan jantan di catur danau Bali adalah alometrik negatif dan ikan betina menunjukkan pola pertumbuhan alometrik negatif di Danau Tamblingan dan Danau Beratan dan pola pertumbuhan isometrik di Danau Buyan dan Danau Batur. Spesies ikan ini berada dalam kondisi baik di catur danau Bali dengan nilai faktor kondisi berkisar antara 0,47–1,54 dengan rerata 0,92 untuk ikan jantan dan 0,43–2,94 dengan rerata 1,12 untuk ikan betina. 
VEGETASI POHON DAN PERSEBARANNYA DI TAMAN WISATA ALAM GUNUNG TUNAK DAN HUTAN KERAMAT, MANDALIKA, LOMBOK TENGAH, PROVINSI NUSA TENGGARA BARAT Muhammad Mansur
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3867

Abstract

The study was conducted on March 2018 in two locations study, i.e. Mount Tunak Nature Tourism Park (NTP) (6400 m2 plot area) and Keramat Forest (4800 m2 plot area), Central Lombok District, West Nusa Tenggara Province. The objective of the study was to find out the diversity of tree species and its distribution which can be used for forest area management by the local government and can support the establishment of a Special Economic Region in Mandalika as a place for conservation, research, education and scientific tourism. In the total plot of 1.12 hectare, we recorded 1560 individuals, representing 56 species,  47 genera  and 19 families. All stems ≥ 5 cm in diameter were measured and identified. In the plot of Mt. Tunak NTP, we recorded 992 individuals, representing 41 species, basal area was 11.4 m2, 31.7 m3of estimated bole volume, 78.3 tons biomass, 39.2 tons carbon stock and 143.7 tons CO2 sequestration. Five dominant tree species (trunk diameter ≥ 5 cm) recorded with the highest Importance Value Index (IVI) were Aglaia argentea (IVI= 34.3%), Schoutenia ovata (IVI= 33.3%), Drypetes neglecta (IVI= 25.4%), Glycosmis pentaphylla (IVI= 16.4%) and Grewia koordersiana (IVI= 14.9%). The level of diversity of tree species in the Mt. Tunak NTP was classified into a midle level with a Diversity Index (H')= 3.3, while in the Keramat Forest we recorded 568 individuals, representing 37 species, basal area was 19.9 m2, 51.1 m3 of estimated bole volume, 149.8 tons biomass, 74.9 tons carbon stock and 274.9 tons CO2 sequestration. Five dominant tree species were recorded; Dalbergia latifolia (IVI= 69.4%), Grewia koordersiana (IVI= 34.5%), Schoutenia ovata (IVI= 34.3%), Cupaniopsis macropetala (IVI= 18.2%) and Rhus succedanea (IVI= 14.2%). The level of diversity recorded (H')= 2.9 and classified as midle level. The composition of vegetation in Mt.Tunak NTP and Keramat Forest is different (Jaccard Similarity Index was 39.3%).    
EFEK SELENIUM OKSIKLORIDA TERHADAP AKTIVITAS IMUNOMODULATOR DARI EKSOPOLISAKARIDA Lactobacillus plantarum Fifi Afiati; D. C. Agustina; S. Wiryowidagdo; Kusmiati Kusmiati; Atit Kanti
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3875

Abstract

Lactobacillus plantarum (LAB) is a group of lactic acid bacteria that can produce exopolysaccharide (EPS). The objective of this study was to determine the effect of adding selenium chloride oxide (SeOCl2) to the increased immunomodulatory potential of EPS L. plantarum Y-1.2. The experiment was conducted in vivo using macrophage cells of peritoneal fluid of mice induced by Staphylococcus aureus. EPS was obtained by precipitation at 4 °C. Identification test using HPLC showed that crude EPS of L. plantarum Y-1.2 containing glucose monomers for value retention times 4.198 and 4.204, crude EPS approaches the retention time of glucose BP 4.234. Testing of immuno-modulatory divided into six treatment groups. Group 1-3 as the control group, a positive control (Stimuno), negative control (crude EPS 50 mg/kg BW in mice), normal control (distilled water). Group 4-6 as the test group was given crude EPS 50 mg/kg BW in mice and selenium oxide chloride doses of 0.13 µg; 0.26 µg; 0.52 µg. The test results showed a dosage of 0.52 µg selenium chloride oxide to crude EPS of 50 mg/kg BW in mice could increase the activity and phagocytic capacity amounted to 28.06% and 28.39%
Karakter Morfologi Dan Anatomi Sawilangit (Vernonia Cinera L.) Pada Ketinggian Yang Berbeda Sri Lestari; Dian Palupi; Riska Desi Aryani
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.4015

Abstract

Vernonia cinerea L. (sawilangit) is a wild plant that has potential as a medicinal plant because of the secondary metabolites in each organ. This research aimed to explore the morphological and anatomical characters of the sawilangit that grow at different altitudes in Banyumas, Cilacap, and Purbalingga. Plant samples were taken using the purposive random sampling method at an altitude of <350 masl, 350–700 masl, and >700 masl. Sawilangit growing at <350 masl has superior morphological and anatomical characters than sawilangit growing at the other two altitudes, which have the characteristics of taller, wider leaf, larger stem diameters, and darker flower color. The observation on quantitative of morphological and anatomical characters showed that sawilangit at <350 masl has an average height of 66.17 cm, root diameter of 0.33 cm, stem diameter 0.32 cm, leaf length and width of 5.7 cm and 2.14 cm, and stomata index of 0.28. These results indicated that altitude <350 masl is suitable for sawilangit growth because it can produce optimal morphological and anatomical characteristics. 
Bioprospection of Enggano Macroscopic Fungi as Antibacterial and Antioxidant Agents Evana Evana; Kartika Dyah Palupi; Listiana Oktavia; Ahmad Fathoni
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.4110

Abstract

Macrofungi in Indonesia have not been widely studied for their pharmacological activity, especially as a source of antibacterial and antioxidant properties, even though Indonesia as a tropical country has quite a high diversity of macrofungi. This study aims to reveal the potential of macrofungi from the Enggano forest as a source of antibacterial and antioxidant compounds. Four types of macrofungi were collected and their metabolites were extracted using four types of organic solvents. Antibacterial and antioxidant activity assay of the extract was carried out using the TLC Bioautography method. From the sixteen macrofungal extracts, there is one extract that has the strongest antibacterial activity compared to the others, namely n-hexane Coriolopsis polyzona. It showed moderate antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC values of 256–128 μg/mL, respectively. Meanwhile, the antioxidant activity of the macrofungal extracts showed weak activity with IC50 values of 3080–7370 μg/mL (AAI values of 0.033–0.079).
Studi Morfologi, Ontogeni, Dan Strategi Reproduksi Pada Melanoides Tuberculata (Müller , 1774) Dan Stenomelania Punctata (Lamarck, 1822) (Gastropoda: Cerithioidea: Thiaridae) Nur Rohmatin Isnaningsih; Ristiyanti M. Marwoto; Alfiah Alfiah; Riena Prihandini; Pramono H. Santoso
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.4088

Abstract

The freshwater Melanoides tuberculata (Thiarid) has wide distribution and have many variation in color, size, and scluptured of their shells. Comparing with another Thiarid so called Stenomelania punctata, shell of M. tuberculata similar in having turreted shape, blackish color, number of whorls, and size of the shells. This study aim to compare more detailed between M. tuberculata and S. punctata based on their morphology, ontogeny and type of reproduction. Ontogeny studies of these two species show that M. tuberculata produces juveniles in embryonic shell form during their reproduction. This embryonic shell is nourished and developed in the subhaemocoelic brood-pouch organ as evidenced by being found in the size range 0.12–5.95 mm. One individu M. tuberculata can produce 1–66 embryonic shells. Meanwhile, in subhaemocoelic brood-pouch of S. punctata only the unshell embryo was seen and embryonic shell was not found. The difference of reproduction system determines the reproductive strategy in both species. M. tuberculata conduct euviviparity reproduction whereas S. punctata is ovoviviparous that releases juveniles in free-swimming veliger form.
Kriopreservasi Tunas in Vitro Pepaya ‘Sukma’ Dengan Perlakuan Prakultur, Loading, Dan Dehidrasi Dengan Pvs2 Dan Modifikasinya Fitri Fatma Wardani; Joko Ridho Witono; Darda Efendi; Diny Dinarti
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.3999

Abstract

Papaya has high genetic variability because it is an open-pollinated plant and has genotype and phenotypeare that are easily changed due to environment changes. Cryopreservation is a storing method of germplasm in liquid nitrogen (-196 oC) which can maintain the genotype and phenotype of germplasm. The experiment aimed to obtain the best preculture, loading, and dehydration for cryopreservation of papaya ‘Sukma’ in vitro shoots. For preculture, we planted shoots on MS media with 0.3 M and 0.4 M sucrose for 1, 2, and 3 days. In the loading treatment, we immersed shoots in loading solution (liquid MS+1.2M glycerol+0.4M sucrose) for 0, 10, 20, and 30 minutes. For dehydration, we immersed shoots in cryoprotectant (PVS2 and its modification) for 5, 10, and 15 minutes. Then, shoots were immersed in liquid nitrogen. The results showed thatshoots had the best survival rate while they had been precultured on MS medium with 0.3 M sucrose for 3 days. The best loading treatment time was 20–30 minutes. The best dehydration treatment was obtained by modification of PVS2 for 10 minutes. The shoots have not been able to recovery after cryopreservation, so it can be concluded that cryopreservation of in vitro papaya ‘Sukma’ shoots has not been successful.
The Opportunity of Developing Microalgae Cultivation Techniques in Indonesia Evi Siti Sofiyah; Iva Yenis Septiariva; I Wayan Koko Suryawan
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.4000

Abstract

The rate of population growth which is relatively rapidly increasing in Indonesia, will require increased fuel. The depletion of the availability of fossil fuels causes the search for the other natural resources needed to become a renewable energy source. One of the significant changes today is microalgae. The application of the algal aquaculture system has been widely applied in the world. The media used in cultivation also varies, one of which is wastewater. The composition of biodiesel energy in Indonesia is increasing and is starting to become the people's choice. Indonesia, which is rich in natural resources, especially the high biodiversity of microalgae, causes microalgae's potential use to be very high. Many studies report the explosion of algal participation in many parts of Indonesia. Research concerning the cultivation of microalgae has been widely successful in Indonesia. The use of microalgae is already available in the field with domestic water treatment applications. The conversion of microalgae into biodiesel also successfully met the requirements of SNI 04-7182-2006.
Effectiveness of Frangipani Leaf Extract (Plumeria alba Linn.) as a UV Filter Irein Kusuma Angreini; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.3951

Abstract

UV filters are necessary since UV rays are harmful to human skin. UV filter compounds in commercial sunscreen products can cause adverse effects. Thus, natural UV filters containing flavonoids, e.g. Frangipani (Plumeria alba Linn.) leaves, are needed. This research examined the use of flavonoids from P. alba leaf as a UV filter with in vitro and in vivo. Maceration was performed with 70% ethyl alco-hol and liquid-liquid extraction for flavonoid purification. Parameters tested were total flavonoid compounds, SPF value, and clinical symptoms of the melanogenesis inhibition scoring. The purification result was 43.6%. The flavonoid concentration in pure extract (64.4 mg QE /g) was higher than crude extract (57.6 mg QE/g). The highest SPF value (33.88) was observed in pure extract at 1400 mg/mL, while the same concentration of crude extract resulted in a lower SPF value (33.06). Pearson correlation analysis (0.536) indicated a di-rectly proportional relationship between SPF value and extract concentration. In vivo analysis was performed by scoring between 0-4 following the severity of erythema. After 21 days of sunlight exposure, the worst erythema was found in the negative control group (score 1.292). The best result was found in the group treated with pure extract 1400 mg/ml (score 0.542).

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