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BERITA BIOLOGI
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Articles 2,125 Documents
UJI TOKSISITAS ORAL REPEATED DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) MENGGUNAKAN MODEL TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR Laksmindra Fitria; Rosita Dwi Putri Suranto; Indira Diah Utami; Septy Azizah Puspitasari
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3936

Abstract

Hairy fig is a tropical medium-sized tree that produces abundant fruits throughout the year. In some Asian countries, the fruits are consumed as traditional medicine and food ingredient. Meanwhile in Indonesia there has not been much use. A series of oral toxicity tests must be conducted to study the possibility of toxic effects and the safety before further exploration. Oral single dose toxicity study of young and ripe hairy fig fruit filtrate has been carried out. Results demonstrated no observed adverse effect level (NOAEL) at a concentration of 100%. This study was aimed to continue the oral toxicity test with repeated dose following standard toxicity procedure by OECD Test Guideline No. 407 with some modifications. Parameters observed were mortality, sublethal effects consisted of physical conditions and behavior, body weight, core temperature, complete blood count, as well as liver, heart, and renal functions by measuring ALT, AST, and creatinine, respectively. Sampling points on day 0, 7, 14, 21, and 28. Results showed no observed adverse effect level (NOAEL) in both young and ripe fruits filtrate at a concentration of 100% however with a tendency to cause anemia and associate with renal dysfunction. Therefore, it is necessary to perform similar method of toxicity test but with lower concentration, also continue with further toxicity tests (subchronic and chronic periods).  
STUDI POTENSI TANAMAN TEBU IRENG (Saccharum officinarum L.) SEBAGAI ANTIOKSIDAN DAN ANTIMIKROBA Putri Sri Andila; I Putu Agus Hendra Wibawa; I Nyoman Lugrayasa; Wawan Sujarwo
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3924

Abstract

Tebu ireng (Saccharum officinarum L.) merupakan jenis tebu lokal yang memiliki ciri khusus yaitu warna batangnya yang hitam.Secara tradisional tebu ireng dimanfaatkan sebagai obat penyakit diabetes.Selain dapat dimanfaatkan secara tradisional sebagai obat diabetes, diyakini tebu ireng masih banyak menyimpan manfaat lain yang belum banyak diketahui. Studi ini dilakukan untuk mengetahui efektivitas ekstrak tebu ireng sebagai antioksidan dan antimikroba, ditinjau dari beberapa bagian tanamannya. Proses ekstraksi menggunakan metode maserasi dengan pelarut methanol, uji aktivitas antioksidan dilakukan dengan metode DPPH free radical scavenger menggunakan spektrofotometer UV-Vis. Pengujian aktivitas antimikroba dilakukan dengan menggunakan metode difusi agar (Kirby-Bauer disc diffusion method). Hasil studi menunjukkan bahwa tebu ireng memiliki kemampuan sebagai antiokidan.Tingkat kepekatan warna dari tebu ireng berkorelasi dengan aktifitas antiokidannya.Ekstrak dari keseluruhan bagian tanaman tebu ireng efektif untuk menghambat pertumbuhan bakteri Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa dan Streptococcus mutans, namun tidak efektif menghambat pertumbuhan jamur Candida albicans.Tebu ireng mengandung pigmen Antosianinserta kaya akan serat pangan .
Rafflesia pricei MEIJER (RAFFLESIACEAE): A NEW LOCALITY IN BORNEO Dewi Lestari; Ridha Mahyuni; Rajif Iryadi
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3856

Abstract

Rafflesia pricei is an endemic species from Borneo. Previously, this species had been reported from Sabah, Sarawak, Brunei Darussalam, East Kalimantan (Now, North Kalimantan). A new locality of the R. pricei was found in Pa’ Pulid forest, near to Pa’ Api village, Krayan Nunukan, North Kalimantan. Most vegetation in this area is dominated by Saurauia sp. (Actinidaceae), Knema sp.(Myristicaceae), Arecaceae, Fern, Zingiber sp. (Zingiberaceae) and Araceae. The soil in the area has a brown color, the pH is 6, the texture is dominated by clay (54%), and the soil moisture 80%. 
THE FUNCTIONAL CHARACTER OF Auricularia auricula CRUDE POLYSACCHARIDES: ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY Rizki Rabeca Elfirta; Iwan Saskiawan
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3988

Abstract

The food stuffs can be clasified as functional food since the foods can improve the human health. One of them are the food stuffs which have function as antioxidant and antibacterial. These activities were studied on crude polysaccharides from Auricularia auricula. The sample was subsequently isolated using hot water and 1 M NaOH to obtain water and alkali soluble of crude polysaccharides. The antioxidant activity was evaluated using β-caroten-linoleat assay. The results showed that the alkali soluble of crude polysaccharides had the highest antioxidant activity (85.82%) at 350 μg/ml. The water and alkali soluble of crude polysaccharides from A.auricula were evaluated for their antibacterial activity using disc diffusion method. The alkali soluble of crude polysaccharides was found to have the highest antibacterial activity at 100 mg/ml against Staphylococcus aureus InaCC B4 and Escherichia coli InaCC B5 with clear zone values of 3.18 mm and 5.10 mm, respectively. The findings indicated that the alkali soluble of crude polysaccharides from A. auricula could potentially be used in part of well-balanced diets and could be considered as a potential source of natural antioxidant and antibacterial products.  
CATATAN PERKEMBANGBIAKAN MELIPHAGA DADA-LURIK (Microptilotis reticulatus) DI PULAU TIMOR DAN INFORMASI TERHADAP PERDAGANGANNYA Oki Hidayat
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3675

Abstract

Streak-breated honeyeater (Microptilotis reticulatus)  is one of the endemic honeyeater birds from Meliphagidae family. Biological information on this species is very limited and poorly known. Field observation was carried out on the active nest found in 2 October 2013. In addition, investigation on social media (facebook) was carried out to examine the exploitation on this species. The results shows that the  cup-shaped nest is 5.6 cm in diameter and 4.9 cm in depth, made by wood fibre, dry grass, glued by cotton fibre from plants and spider web on small branch. Nowadays M. reticulatus is trapped and traded without any regulation from management authority. As many as 651 individuals has been trapped and sold between 2016 and 2019.  
CHARACTERIZATION OF SUPERNATANT EXTRACT AND VIABILITY OF BACILLUS SUBTILIS KM16 AND PSEUDOMONAS SPP. IN FISH FEED AS BIOCONTROL AGENTS AGAINST AQUACULTURE PATHOGENS Stella Magdalena; Brenda Kristanti; Yogiara Yogiara
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3973

Abstract

The use of biocontrol agent in aquaculture is being adapted as an effective alternative to antibiotics which can lead to the elaboration of antibiotic-resistant bacteria and confer unpleasant impacts to aquatic organisms. Aquatic bacteria have been discovered as biocontrol agents and potential probiotic candidates to improve the health of aquatic organisms, feed efficiency, and disease resistance to aquaculture pathogens. However, local isolate has not intensively been explored and used to increase aquaculture sector productivity. Therefore, this research aimed to determine minimum inhibitory concentrations of their antibacterial compounds against aquaculture pathogens and to characterize aquatic bacteria by their viability in the feed. Four isolates from several aquatic environments in Indonesia (Pseudomonas sp. S1.1, Pseudomonas sp. S1.2, Pseudomonas sp. SL1.1, and Bacillus subtilis KM16) were used to characterize of antibacterial compound and to determine the viability in feed. Ethyl acetate extracts from all isolates showed better antibacaterial activity against Aeromonas hydrophila and Vibrio vulnificus than chloroform and dichloromethane extracts, in which ethyl acetate extract from Bacillus subtilis KM16 showed the strongest antibacterial activity. Pseudomonas spp. were more effective against V. vulnificus (40 mg/mL) and Bacillus subtilis KM16 was more effective against A. hydrophila (20 mg/mL), as proved by the minimum inhibitory concentrations of their ethyl acetate extracts. In this research, Bacillus subtilis KM16 had stable viability in feed than Pseudomonas sp. isolates. 
HUBUNGAN PANJANG-BOBOT, FAKTOR KONDISI DAN KARAKTERISTIK BIOMETRIK IKAN LELE AFRIKA (Clarias gariepinus) ALBINO ASAL THAILAND Bambang Iswanto; Rommy Suprapto; Pudji Suwargono
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3921

Abstract

The African catfish (Clarias gariepinus) has been introduced several times to Indonesia, including from Thailand (Paiton strain). The breeding of Paiton strain resulted in albino individuals. The present study aimed to investigate the length-weight relationship, condition factor and biometric characteristic of those albinos. Five pairs of each albino and normal coloured Paiton African catfish were artificially bred. Larvae and juveniles from each pair were reared one month of larval rearing phase, one month of nursery phase and two months of grow-out phase, then the total length and body weight were measured for length-weight relationship and condition factor analysis, finally the biometric was characterized. The present study revealed that the relationship between total length (L) and body weight (W) of the Paiton African catfish was positive allometric (W = 0.0038L3.23 in the albino and W = 0.0027L3.27 in the normal coloured one). The albino Paiton African catfish was more rotund (condition factor of 0.79±0.07) than the normal colored one (condition factor of 0.68±0.06). Biometrically, the albino Paiton African catfish has a bigger head portion and fewer dorsal and anal fin rays than those of the normal coloured one. Despite the colour difference, the albino and normal coloured Paiton African catfish were morphologically different.  
KEANEKARAGAMAN DAN KOMPOSISI SPESIES MAKROALGA LAUT PADA TIPOLOGI PANTAI YANG BERBEDA DI KAWASAN PESISIR GUNUNGKIDUL D.I. YOGYAKARTA Dwi Sartika; Abdul Razaq Chasani; Ajeng Meidya Ningrum; Septi Lutfiatun Nafiah; Septy Wulan Cahyani
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3941

Abstract

Makroalga laut merupakan alga makroskopis yang dapat dijumpai di daerah intertidal kawasan pesisir Gunungkidul D.I. Yogyakarta, terdiri dari makroalga hijau (Chlorophyceae), coklat (Phaeophyceae), dan merah (Florideophyceae). Kawasan pesisir Gunungkidul terdiri dari deretan pantai dengan berbagai tipologi sehingga keanekaragaman jenis makroalga lautnya akan berbeda pada tipologi pantai yang berbeda. Penelitian ini bertujuan untuk mengetahui keanekaragaman dan komposisi spesies makroalga laut pada tipologi pantai yang berbeda di kawasan pesisir Gunungkidul D.I. Yogyakarta. Pengambilan sampel dilakukan pada bulan September 2019 hingga Februari 2020 menggunakan metode purposive sampling, kemudian dilakukan pengawetan awetan basah dan identifikasi di Laboratorium Sistematika Tumuhan Fakultas Biologi UGM. Perbandingan komposisi spesies dianalisis berdasarkan Indeks Kesamaan Sorensen. Dari hasil penelitian yang dilakukan didapatkan 37 spesies yang tergolong kedalam 28 genus, 19 family dan 10 ordo, Makroalga laut di wilayah pesisir Gunungkidul di dominasi oleh kelas Florideophyceae dengan persentase kehadiran spesies 43,37% (18 spesies); kelas Chlorophyceae 28,95% (10 spesies); dan Phaeophyceae 23,68% (9 spesies). Komposisi spesies makroalga laut di kawasan pesisir Gunungkidul memiliki tingkat kesamaan yang tinggi (>0,5) walaupun hidup di tipologi pantai yang berbeda. Persamaan tertinggi ditemukan pada makroalga laut yang hidup di pantai dengan tipologi subaerial dan marine deposition coast. 
ISOLASI DAN UJI KOMPATIBILITAS BAKTERI HIDROLITIK DARI TANAH TEMPAT PEMROSESAN AKHIR TALANGAGUNG, KABUPATEN MALANG Prilya Dewi Fitriasari; Nanda Amalia; Susiyanti Farkhiyah
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3828

Abstract

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.   
LARVA TREMATODA PADA SIPUT AIR TAWAR DI AREAL PERSAWAHAN DAERAH ISTIMEWA YOGYAKARTA Soenarwan Hery Poerwanto; Dian Antika Kusuma Dewi; Giyantolin Giyantolin
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3939

Abstract

The life cycle of Digenea subclass trematodes  that mostly involves snails as intermediate host in the larval stage in the form of sporocysts, redia and cercariae. This study aims to determine the species of freshwater snails, populations and stages of trematoda larvae that infect freshwater snails in the paddy fields and also the factors that influence the population of trematoda larvae. This research method uses purposive random sampling. Snails were collected from paddy fields in Sleman Regency, Yogyakarta City and Bantul Regency, Special Region of Yogyakarta. Snails and Trematodes larvae were identified based on morphological characters with a microscope. The results showed that the species of freshwater snail as  an inang antara  of Trematode was Lymnaea sp. (96.9%), Pomacea canaliculata (2.6%), and Brotia sp. (0.5%). The total population of trematoda larvae in freshwater snails was 4329 individuals with the largest population in Sleman Regency (2784 individuals). Trematode larval stages found were sporocysts (4.8%), redia (8.3%), Leptocercous type cercariae (35.2%), and Furcocercous type cercariae (51.7%). The most trematode larvae found in  fresh water snail was  Furcocercous type cercariae. Factor affecting trematode larvae population are the species and abundance of freshwater snail. 

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