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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 11   12   13   14   15 
Isolats Bakteri Indigenous Penghasil Milk-Clotting Protease untuk Fermentasi Keju Rahmani, Nanik; Sari, Yana Nurita; Palupi, Nurheni Sri; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.729 KB) | DOI: 10.14203/jbi.v9i2.166

Abstract

The aims of this research is to isolation of bacteria that potential to produce of milk clotting protease enzymes fromfermented food that will be used as a substitute for rennet in cheese making. There are five food fermentations suchas tauco, tempeh, red oncom, sticky tape, and pickled mustard greens that are used as a source for isolation of bacteriathat could produce milk clotting protease. The results obtained four isolates proteolytic bacteria from two fermentedfood samples, three isolates bacteria from tauco (TCN 1, TCN 2, TCN 3) and one isolate from pickledmustard greens (DSN 1). Based on 16S rDNA, these isolates were identified as Bacillus sp. Bacterial isolate TCN 1has a milk clotting activity of 29.17 U/mL, whereas bacteria isolates of TCN 2, TCN 3 and DSN 1 have activities of70 U/mL achieved at the 24 hours incubation, respectively. The proteolytic activities of bacteria isolates TCN 1,TCN 2, TCN 3 and DSN 1 at the 24 hours fermentation process were 0.0117 U/mL, 0.0021 U/mL, 0.0150 U/mL,and 0.200 U/mL, respectively. The ratio of milk clotting protease activity and the proteolytic activity for bacteriaisolates TCN 1, TCN 2, TCN 3 and DSN respectively were 5402, 175000, 7292, and 3333. This showed that theenzyme from bacterial isolates TCN 2 can be used as an alternative to rennin in cheese making.Keywords: milk clotting protease, cheese, calf rennet, fermentation food
Dampak Kegiatan Manusia Terhadap Keragaman dan Pola Distribusi Kumbang Sungut Panjang (Coleoptera: Cerambycidae) di Gunung Salak, Jawa Barat Noerdjito, Woro Anggraitonongsih
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3066

Abstract

The impact of human activities on the Longhorn Bettles Diversity and Their Distribution Patterns in Mount Salak, West Java, had been conducted by observation and collecting efforts on various types of habitats which include undisturbed and disturbed forests. Longhornbeetles are one of the largest groups of wood borers and therefore typical forest dependent insects. The species diversity and distribution pattern of these beetles may vary with speciescomposition age of the trees, and stability of the forest, and therefore they are a useful bioindicator for assessing forest condition. The beetles were collected using “Artocarpus branchtraps”, a bait trap. It is a bundle of about 5-6 freshly-cut branches of Artocarpus heterophyllus (jackfruit), about 80 cm long with many leaves, tied up and hung from a tree trunk or standingpoles at 1.5 m above the ground. The trap attracted longhorn beetles that need feeding for reproductive maturity. Ten traps were set at each study site (habitat). The attracted beetleswere collected every week by beating method, because in West Java forest area, beetles are usually gathered by the 6th day after trap setting. Light traps were also set up for 2 night, at each habitat for collecting nocturnal longhorn beetles. Totally 578 specimens were collected, consisted 38 species of longhorn beetles, dominated by small body size species less than 10mm. Out of these 38 species, some species were known distributed in certain habitat andelevation, other species have wider distribution, where as some species were endemic and new record for Java.Keywords: distribution, diversity, longhorn beetles, Mount Salak
Distribusi Spasial dan Temporal Ikan Bonti-bonti (Paratherina striata Aurich), Endemik di Danau Towuti-Sulawesi Selatan Nasution, Syahroma Husni
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.76 KB) | DOI: 10.14203/jbi.v5i1.3209

Abstract

ABSTRACTSpatial and Temporal Distribution of Bonti-bonti (Paratherina striata) an EndemicFish in Towuti Lake, South Sulawesi. Bonti-bonti (Paratherina striata) is an endemicfish species in Towuti and Mahalona Lake. This fish included into vulnerable species. Itshould be protected from decreasing of fish population due to increasing exploitationand habitat quality changes. The objective of this research to study on spatial and temporaldistribution of the fish, as a basic information for its conservation. Samples were collectedfrom May 2006 to April 2007 using experimental gillnet mesh size 0.625, 0.75, 1.0, and1.25 inches at five stations. Fish number and size captured during 15 hours. Waterquality parameters that analyzed were temperature, conductivity, pH, and dissolvedoxygen using water quality checker-Horiba, alkalinity used titration methode, whilewater level and rain fall obtained from PT. Inco. Distribution of fish abundance wasanalyzed with Mann-Whitney non-parametric test. Water quality parameters thatinfluencing fish abundance were analyzed by multivariate analysis. The results show thatthe distribution of bonti-bonti spreading widely starting from lakeside to middle of thelake. The highest abundance of the fish are in inlet with sand, gravel, and stone substrat.Fish size was obtained more various at the inlet station than other stasions and it predictedas main habitat. Water qualitiy parameters of were not factors influencing difference ofspatial distribution but it was influenced by behavior of habitat selection. The highesttemporal distribution of the fish abundance in November and December influenced bydissolved oxygen and high water level.Key words : Spatial and temporal distribution, Paratherina striata, endemic fish, abundance,Towuti Lake
Efikasi Vaksin Inaktif Bivalen Avian Influenza Virus Subtipe H5N1 (Clade 2.1.3. dan Clade 2.3.2) di Indonesia Dharmayanti, NLP Indi; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1108.821 KB) | DOI: 10.14203/jbi.v11i2.2190

Abstract

ABSTRACTStatus of avian influenza virus subtype H5N1 in Indonesia until 2014 is still endemic in poultry and recorded, there were two types clade of circulating H5N1 namely clade 2.1.3 and the new introduction of lade 2.3.2 since the end of 2012. Both of the clade of avian influenza viruses subtype H5N1 (clade 2.1.3 and 2.3.2) caused the the  AI vaccination program to control of AI in poultry needs to be evaluated.  In this study, we developed a bivalent AI vaccine (which contains clade 2.1.3 and 2.3.2 viruses as a seed vaccine) that   adapted with  the circulation of AI viruses in the field. Result of the study showed that the bivalent vaccine which developed in this study has good efficacy that was challanged with both of AI clade AI and proven to reduce shedding / viral contamination to the environment. It is expected that  the development of bivalent H5N1 vaccine will increase the effectiveness and efficacy of vaccination programs to control highly pathogenic avian influenza disease in Indonesia. Keywords : avian influenza virus, clade, vaccine, bivalent 
Aplikasi Kajian DNA Molekuler dan Fenotipik Pada Program Pelepasliaran Burung Kakatua Zein, Moch Syamsul Arifin; Haryoko, Tri; Fitriana, Yuli Sulistya; Sulistyadi, Eko; Prawiradilaga, Dewi Malia
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (587.497 KB) | DOI: 10.14203/jbi.v13i1.3105

Abstract

ABSTRACTThere are six species of cockatoos in Indonesia which are well known as exotic, smart, and they can be trained in a variety of attractions. Thus, many people want to keep those birds as pets. All of pets which have been kept by community should be evaluated from various aspects before being reintroduced to their natural habitat. The examination of sex and species of illegal cocktoos play as a key role for the reintroduction programme. Theobjective of this study was to evaluate the reliability and effectivity of evaluation technique of morphometric and molecular for reintroduction programme of cockatoos. We used the COI gene sequences from 68individuals of cockatoos from pet communities in and around Jakarta and four sequences from GenBank. The phylogenetic analysis used the neighbor-joining method, in which the genetic distance matrix calculations with Kimura 2-parameter models that are implemented on a pairwise distance calculation in the MEGA program version 6:05. The result of the genetic variation of the cockatoo species which shows intraspecific divergence was Cacatua alba (n=4)= 0%, C. galerita (Australia n=9)= 0.6%, C. galerita (Indonesia n=53)= 0.3%, C .goffiniana (n=3)= 0%, C. moluccensis (n=7)= 0.1%, and C. sulphurea (n=2)= 0.3%, with a range of 0-0.6%. The results indicate that the average of intraspecific of COI in the cockatoos community was 0.25±0.055%, and interspecific divergences ranged from 3.1 to 11.6%. The phylogenetic tree shows the monophyletic clade of cockatoo species in Indonesia. In addition, DNA barcode analysis and molecular sexing could correct the error and doubts the result of five individual species identification and two individual sexing identification of C.galerita by morphological identification. The results of morphological examanation base on body weight, body length and head-bill length of C. galerita triton were not significantly different (P?0,5). Finally, 19 individuals C. galerita triton and two individuals P. aterrimus were reintroduced to their natural habitat.Keywords: cockatoo, barcodes DNA, reintroduction
Distribusi Kambing Hutan Sumatera[CapricornrS sumatraensis sumatraensis (Bechstein, 1799)] di Sipurak, Taman Nasional Kerinci Seblat, Sumatera Susanti, Neneng; Mardiastuti, Ani; Andayani, Noviar
JURNAL BIOLOGI INDONESIA Vol 4, No 2 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i2.3269

Abstract

ABSTRACTSumatran Serow Capricornis sumatraensis sumatraensis (Bechstein, 1799))Distribution at Sipurak-Kerinci Seblat National Park. Sumatran serow [Capricornissumatraensis sumatraensis (Bechstein, 1799)J is one of endangered species in KerinciSeblat National Park (KSNP). Few data are available about Sumatran serow in KSNP.The research was designed to analize the distribution of Sumatran serow in Sipurak.Fieldstudy was conducted from September 2005 to February 2006 in Sipurak, KSNP.The detection-non detection survey method was applied for observation. There were 44cells sampling, the number of cells determined by purposive sampling base on habitattypes and accessibility to reach the location of cell (easy or hard). The data were analyzedby Arcview 3.2 PC. The sumatran serow just indirectly detected. Sign of its existencemainly detected at ramp. Distribution of sumatran serow mainly was invented on the lowland forest type (8 1,82%) which are characterized by caves, rock-cliff, and rugged hills.Key words: Distribution; detection-non detection; sumatran serow; Sipurak
Potensi Serapan Gas Karbondioksida (CO2) Pada Jenis-Jenis Pohon Pelindung Jalan Mansur, Muhammad; Pratama, Bayu Arief
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2079

Abstract

A Study of Potential Absorption of Carbon Dioxide (CO2) in Wayside Trees in Bogor City around was conductedin April to May 2013 by using a portable LCi ADC Bioscientific Ltd. Photosynthesis System. Inventory of waysidetrees species in the site was done by surveys, and sixty-three samples from twenty-one species of wayside trees havebeen measured. This study for determined the ability of CO2 absorption in each species of wayside trees species.Twenty one species of wayside trees were recorded. Angsana (Pterocarpus indicus), Mahogany (Swieteniamacrophylla), Walnuts (Canarium indicum), and Tanjung (Mimusops elengii) were common wayside trees planted inBogor and surrounding areas. The highest rate of CO2 absorption was recorded for Kersen (Muntingia calabura) onaverage of 23.92 μmol/m2/s , followed by the Bintaro (Cerbera manghas) 16.42 μmol/m2/s, Dadap (Erythrina cristagalli)16.38 μmol/m2/s, and Trembesi (Albizia saman) 15.87 μmol/m2/s. A common planted species in the rangebetween 8 to 13 μmol/m2/s, such as; Angsana (Pterocarpus indicus) 12,79 μmol/m2/s, Walnuts (Canariumindicum) 9,39 μmol/m2/s, Tanjung (Mimusops elengi) 8,72 μmol/m2/s and Mahogany (Swietenia macrophylla) 8,30μmol/m2/s. While the lowest CO2 absorption is Burahol (Stelechocarpus burahol) 7.16 μmol/m2/s, BungaSaputangan (Maniltoa grandiflora) 7.89 μmol/m2/s and Damar (Agathis alba) 7.86 μmol/m2/s. There were positivecorrelation between transpiration rate with CO2 absorption rate and stomatal conductance on all the tree specieswere measured.Keywords: CO2 absorption, wayside trees, Bogor.
Eksplorasi Keanekaragaman Aktinomisetes Tanah Ternate Sebagai Sumber Antibiotik Nurkanto, Arif; Listyaningsih, Febrianti; Julistiono, Heddy; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.268 KB) | DOI: 10.14203/jbi.v6i3.3141

Abstract

ABSTRACTExploration of Soil Actinomycetes Diversity from Ternate as Indigenous Antibiotic Sources.Actinomycetes of soil samples from Ternate, North Moluccas were isolated using SDS-YEmethod in humic acid vitamin agar. Ternate has high abundance of Actinomycetes, approximately6.00 – 487 x 104 CFU/ g soil, depends on habitat types. We have selected 60 isolates andconducted antibiotic screening against pathogenic bacteria and fungi using agar diffusionmethod and found both narrow and broad antibiotic spectrum types . Based on 16S rDNAanalysis, all Actinomycetes with antibiotic activities are belong to the genus Streptomyces. .Minimum Inhibitor Concentration (MIC) value was determined by broth microdilution method.It was found that MIC values varied, depended on microbial tested. We found two isolateswith higher antibiotic activity compared to the commercial antibiotics (chloramphenicol,erythromycin for antibacterial and nystatin, kabicidin for antifungal). Cell destruction analysiscaused antibiotic activities was conducted through leak of protein and nuclatic acid.Key words : Actinomycetes, soil, Ternate, antibiotic, cell distruction
Effect of Tokay Gecko (Gekko gecko LINNAEUS, 1758) Saliva on Angiogenesis During Wound Healing Phase of Autotomized Tail in Common Sun Skink (Eutropis multifasciata KUHL, 1820) Inayah, Nurul; Soesilo, Nyoman Puniawati; Pratiwi, Rarastoeti
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1344.355 KB) | DOI: 10.14203/jbi.v13i2.3399

Abstract

ABSTRACTThe purpose of this study was to investigate the effect of Tokay gecko saliva on morphology and angiogenesis response on the healing process of skink tail wound and also to characterize the protein profile of Gecko saliva. Twelve skinks were autotomized and wound surface of tail smeared by young gecko saliva, adult gecko saliva, and human’s saliva twice per day and control. The morphological changes of the wound surface were observed. The angiogenesis response was observed in vitro using Chorioallantois Membrane (CAM) of the ninth days chick embryos. Protein profile of gecko saliva analyzed with SDS-PAGE. Generally, treated wound showed a better healing. Young gecko saliva able to stimulate angiogenesis in wound healing stage of sun skink tail after autotomy. Saliva protein of young and adult Gecko differences was not only in the size (or density) but also in the number of the bands. The young and adult Gecko revealed a striking consistency of protein patterns, indicating a profound physiological stability of the whole saliva. This study showed that Gecko saliva able to accelerate wound healing, thus Gecko saliva may have potential me as a future therapeutic for treating infected wounds.Keywords: Tokay gecko saliva, wound healing, sun skink tail, angiogenesis, protein profile.
Penapisan dan Karakterisasi Rhizobakteria serta Uji Aktivitasnya dalam Mendukung Perkecambahan dan Pertumbuhan Benih Jagung (Zea mays L.) Agustiyani, Dwi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2890

Abstract

ABSTRACTPlant Growth Promoting Rhizobacteria (PGPR) are a group of bacteria known to influence plant growth by direct or indirect mechanisms. In search of efficient PGPR which potential as biofertilizers, a total of 26 isolates of rhizobacteria were isolated from various plant rhizosphere. These 26 isolates were screened through in vitro methods for PGPR properties like phosphate solubilization, siderophore, IAA and amonia production, and catalase activity. The result revealed that 9 isolates showed positive activities for phosphate solubilization 17 isolates for IAA production 18 isolates for siderophores 19 isolates for ammonia production and all of the isolates have catalase activities . Only four isolates (Az.KT.CSC, Az.D.8B, Az.D.8A and Az.Lo.10B) exhibited multiple plant growth promoting traits viz., phosphate solubilization, siderophore, IAA, amonia production, and catalase activity. Ten isolates that have different characters were further investigated for quantitative analysis of IAA production, HCN production and its effect on germination and seedling growth of Zea mays. The range of IAA production was 3,12– 134,27 ppm, among ten isolates, Az.D.8B isolate produced the highest IAA (134,27 ppm). Production of HCN was detected in eight isolates. Four isolates positively affected the germination of Zea mays seeds. Highest root elongation was recorded when seeds were treated with Az.Lo.5 isolate. Whereas, the highest chlorophyl content and plant high were recorded when seeds were treated with Az.B.8B isolate.Keywords: N-fixing bacteria, PGPR, IAA, siderophore, catalase

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