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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 18 Documents
 1   2 
Search results for , issue "Vol 23 No 1 (2022)" : 18 Documents clear
Intensitas Latihan Fisik Ringan-Berat Kurang Memengaruhi Kadar Follicle Stimulating Hormone Serum Mencit Betina Anggis Putri Wijayanti; Lilik Herawati; Endyka Erye Frety; Zakiyatul Faizah
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.048 KB) | DOI: 10.19087/jveteriner.2022.23.1.10

Abstract

Physical exercise can cause a change in hormonal system resulting an irregularities of menstrual cycle. Menstrual irregularities is one of the ovulation disruption symptoms. The most important cause of ovulation disruption is a decrease of Gonadothropin Releasing Hormone (GnRH) which is regulated by hipothalamus-pitutary-ovarium axis (HPO axis) mechanism resulting in decreased of Follicle Stimulating Hormone (FSH) secretion. HPO axis mechanism disruption can be caused by severe physical activity. Methods: This research took place in phaculty of veterinary of Airlangga University. The methods of this research was experimental laboratory study with Randomized Post-test Only Control Group Design. The number of sample were 28 female mice divided into four groups with a sampling technique using probability sampling with the type of simple random sampling. The independent variable of this research were moderate and severe physical exercise. The dependent variable were serum Follicle Stimulating Hormone (FSH) levels. The data were analyzed using SPSS 25 software with one way- ANOVA test. This research was conducted to analyze the difference effect of low to severe physical exercise on serum Follicle Stimulating Hormone (FSH) levels in female mice. The result is there were no significant difference in levels of Follicle Stimulating Hormone (FSH) mice with low, moderate and severe physical activity. The conclusion that can be taken in this research is there is no difference in the effect of low, moderate to severe physical exercise on serum Follicle Stimulating Hormone levels in female mice.
Deteksi Gen Penyandi Resistansi Tetracycline dan Plasmid Mediated Quinolones pada Salmonella Ayam di Bandung dan Purwakarta Leila Nur Azizah; Agustin Indrawati; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.554 KB) | DOI: 10.19087/jveteriner.2022.23.1.55

Abstract

This study aimed to identify genes encoding tetracycline and plasmid-mediated quinolones resistance to Salmonella spp from Poultry Farm in Bandung and Purwakarta, West Java. A total of 70 samples were collected from poultry farm In Bandung and Purwakarta, West Java. All isolates were test by selective media (SSA) and confirmation Salmonella with PCR. Thirty three isolate positive from selective media Salmonella Shigella Agar (SSA) and 21 isolat was confirmed as Salmonella spp by PCR. Twenty one isolate isolated were tested for tetracycline, doxycicline,, nalidixic acid, oxytetracycline, enrofloxacyn using disk diffusion method. TE-resistant were screened for presence of tet(A) and tet(B) genes by single polymerase chain reaction (PCR). The qnr(A), qnr(B) and qnr(S) genes were detected by multiplex PCR in quinolone resistant Salmonella isolates. The result of antibiotic sensitivity test showed that resistance to ampicillin (95.2%), tetracycline (100%), oxytetracycline (95.2%), nalidixic acid (90.4%), eritromisin (85.7%), enrofloxacin (76.2%), Gentamisin 47.6%, chloramphenicol (38.1%). The distribution of antibiotics-resistance genes in the Salmonella isolates included ampC (95.2%), tet(A)(61.9%), tet(B)(38.1%), qnr(A)(28.5%), qnr(B)(14.3%) and qnr(S)(23.8%).This study shows that a few pathogens of Salmonella are resistant to ampicillin, tetracycline , and quinolone. The tet and qnr genes are responsible for this resistance among Salmonella in Bandung and Purwakarta, West Java Indonesia was high.
Morfologi dan Morfometri Sel Darah Rusa Timor (Rusa timorensis) di Penangkaran Virgilius Martin Kelake Kedang; Anita Esfandiari; Arief Purwo Mihardi; Robby Wienanto
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.145 KB) | DOI: 10.19087/jveteriner.2022.23.1.99

Abstract

Rusa timor (Rusa timorensis) merupakan salah satu jenis rusa asli Indonesia. Salah satu aspek penting dalam upaya konservasi adalah terkait aspek kesehatan rusa timor. Nilai normal maupun gambaran rujukan terkait karakteristik fisiologis hewan dinilai penting dalam mengevaluasi status kesehatan rusa timor di penangkaran. Salah satu aspek yang penting untuk dipelajari yaitu karakteristik morfologi sel darah. Penelitian ini bertujuan mengetahui morfologi dan morfometri sel darah rusa timor di penangkaran. Sampel darah diperoleh dari 10 ekor rusa timor yang terdiri dari lima ekor rusa timor jantan dan lima ekor rusa timor betina. Sampel darah dipreparasi dalam sediaan ulas darah dengan pewarna Giemsa kemudian diamati di bawah mikroskop. Pengukuran morfometri sel darah dilakukan menggunakan mikroskop digital. Morfologi eritrosit rusa timor pada sediaan ulas darah memperlihatkan adanya poikilositosis berupa sel sabit. Morfometri eritrosit rusa timor jantan secara nyata (P<0,05) lebih besar dibandingkan dengan rusa timor betina. Gambaran morfologi leukosit rusa timor sama dengan leukosit mamalia pada umumnya. Morfologi dan morfometri masing-masing jenis leukosit rusa timor tidak memiliki perbedaan nyata (P>0,05) antar jenis kelamin
Analisis Infektivitas Simian-Human Immunodeficiency Virus pada Peripheral Blood Mononuclear Cell Macaca fascicularis dan M. nemestrina Secara In Vitro Gede Eko Darmono; Silvia Triwidyaningtyas; Budiman Bela; Diah Iskandriati; Dondin Sajuthi; Joko Pamungkas
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.183 KB) | DOI: 10.19087/jveteriner.2022.23.1.16

Abstract

Development of human immunodeficiency virus type 1 (HIV-1) vaccines and anti-retroviral treatmentis currently hindered by the lack of models representing prominent symptoms of HIV-1 infections seen in humans. Simian-human immunodeficiency virus (SHIV) was constructed to resolve the limitations of SIVmac model and has been used in nonhuman primate model ofviral infections, particularly infections by the close relatives of HIV-1. Macaca fascicularis and M. nemestrina are being developed as model HIV/AIDS, by using chimeric virus SHIV produced by replacing the nucleotide structure of cyclophilin A binding region, vif gene and nef of HIV-1 with cyclophilin A binding region, vif gene and nef from SIV. The research aims to study the model of HIV/AIDS on nonhuman primates PBMC in vitro using SHIV. In particular, the study aims to obtain information about the capability of SHIV replication in PBMC of M. fascicularis and M. nemestrina. Results showed a cytopathic effect (CPE) in the form of multinucleated giant cells and expression of p24 protein in PHA-stimulated PBMC cultures of M. fascicularis and M. nemestrina after SHIV infection. The conclusion of this study is that SHIV can infect PBMC M. fascicularis and M. nemestrina in vitro based on CPE and expression of p24 protein.
Peningkatan Performa Reproduksi Induk Babi Melalui Pengaturan Penyapihan Anak Babi Ni Luh Gde Sumardani; Komang Budaarsa; Anthonius Wayan Puger
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (22.718 KB) | DOI: 10.19087/jveteriner.2022.23.1.64

Abstract

This study aims to determine the optimal timing of weaning piglets from the sows so that the sows can return to estrus, thereby increasing the efficiency of production and reproduction of these sows. The design used a randomized block design (RBD) with three treatments, the piglets weaned at 25 days (A), 28 days (B), and 30 days (C) in three pig farms. Each treatment consisted of three replications, and each replication consisted of two of the sow. The variables observed: litter size, bodyweight of weaned piglets, estrus after weaning, and the percentage of mortality of piglets after weaning. The results showed that the bodyweight of the weaning piglets and the mortality after weaning were 6.83 kg; 1.33% (A), 8.17 kg; 1.17%(B), and 9.0 kg; 1.17% (C) significant (P<0.05) in treatment B; The sows were on estrus after weaning an average of 3.67 days (A and C) and 3.33 days (B) was nonsignificant (P>0.05). The litter size reaches 10.17 head (A), 13.0 head (B) and 11.5 head (C) were nonsignificant (P>0.05). The conclusion of this research is the optimum time for the implementation of weaning piglets is 28 days old in the pig farming business.
Cover, Editorial Board, Daftar Isi I Wayan Batan
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7563.079 KB)

Abstract

Cover, Editorial Board, Daftar Isi
Peningkatan Performa Reproduksi Induk Babi Melalui Pengaturan Penyapihan Anak Babi Ni Luh Gde Sumardani; Komang Budaarsa; Anthonius Wayan Puger
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.6 KB) | DOI: 10.19087/jveteriner.2022.23.1.64

Abstract

Penelitian ini bertujuan untuk menentukan waktu optimal penyapihan anak babi agar induk babi dapat berahi kembali, guna meningkatkan efisiensi produksi dan reproduksi induk babi tersebut. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok dengan tiga perlakuan yaitu penyapihan umur 25 hari (A), penyapihan umur 28 hari (B), dan penyapihan umur 30 hari (C) pada tiga usaha peternakan babi. Setiap perlakuan terdiri dari tiga ulangan, dan masing-masing ulangan terdiri dari dua ekor induk babi. Variabel yang diamati meliputi: litter size, bobot badan anak babi yang disapih, induk berahi kembali pascasapih, dan mortalitas anak babi pascasapih. Hasil penelitian menunjukkan bahwa rataan bobot badan anak babi saat disapih berbeda nyata (P<0,05) pada perlakuan B yaitu: 6,83 kg (A), 8,17 kg (B), dan 9,0 kg (C). Mortalitas pascasapih yakni: 1,33% (A), 1,17% (B), dan 1,17% (C) tidak berbeda nyata (P<0,05) antar perlakuan; Induk kembali berahi setelah sapih rata-rata 3,67 hari (A dan C) dan 3,33 hari (B) tidak berbeda nyata (P>0,05). Rataan litter size mencapai 10,17 ekor (A), 13,0 ekor (B) dan 11,5 ekor (C) tidak berbeda nyata (P>0,05). Simpulan dari penelitian ini adalah waktu optimum pelaksanaan penyapihan anak babi adalah umur 28 hari.
Produksi Rekombinan Bovine Lactoferrin pada Sistem Ekspresi Eschericihia coli Ni Luh Wayan Yulia Mirayanti; I Gusti Ngurah Kade Mahardika; Made Pharmawati
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.111 KB) | DOI: 10.19087/jveteriner.2022.23.1.105

Abstract

Lactoferrin is an 80 kDa glycoprotein which has advantages in biological activity as an antimicrobial, antibacterial, antiviral, antiparasitic, and immunomodulatory agent. Advances in recombinant technology have made it possible to produce lactoferrin proteins on a large scale, the production of recombinant lactoferrin can be carried out in various expression systems. Escherichia coli is one of the hosts in expression systems that are widely used in the production of recombinant proteins. The recombinant protein lactoferrin is produced by insertion of the bovine lactoferrin gene in the plasmid pET 11-a. The bovine lactoferrin gene that has been inserted into the plasmid pET, transformed and expressed in the cell host of E. coli BL21. The bovine lactoferrin protein expression was induced by the addition of a chaperone one of the coexpressions, which accompanied the E. coli synthesis system to achieve the desired protein expression. The expression of pET11a - Bovlacto plasmid in E. coli was supported by the inducer L-arabinose and IPTG. The bovine lactoferrin gene embedded in the recombinant plasmid pET-11a was able to be well expressed in E. coli BL21 with the hybridization signal in the dot blot method test and the target specific band, namely in the 80-85 kDa position range of SDS-PAGE electrophoresis results.

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