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<![CDATA[Hyaluronan Expression on Vitrified Oocytes Before and After In Vitro Maturation (EKSPRESI HYALURONAN PADA OOSIT YANG DIVITRIFIKASI SEBELUM DAN SESUDAH MATURASI IN VITRO) ]]>
<![CDATA[Zakiyatul Faizah]]>;
<![CDATA[R. Haryanto Aswin]]>;
<![CDATA[Hamdani Lunardhi]]>;
<![CDATA[Widjiati Widjiati]]>
<![CDATA[ Jurnal Veteriner Vol 19 No 1 (2018) ]]>
Publisher : <![CDATA[Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association ]]>
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DOI: 10.19087/jveteriner.2018.19.1.71
<![CDATA[Oocyte vitrification is a major challenge in assisted reproductive technology. Oocyte vitrification with cumulus cells provide benefits in the process of maturation and fertilization. Vitrification leads to rapid temperature changes, therefore the decreasing in temperature could damage the cells even when the morphology was normal. Vitrification of mature oocytes is common because of its low sensitiveness towards low temperatures than immature oocytes. The aim of the research was to compare the effect of vitrification before and after in vitro maturation to the expression of hyaluronan. Maturation was operated in medium TC 50 ?L in CO2 incubators for 24 hours. Vitrification started with washing oocyte in PBS basic medium supplemented with 20% serum for 1-2 minutes, then in equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose (K1); 2) PBS + 20% serum + 0.25 M sucrose (K2); and 3).PBS + 20% serum + 0.1 M sucrose (K3). Immunocytochemical stain was performed to evaluate the hyaluronan expression. Remmele scale index (Immunoreactive score, IRS) was used to read the result. There was no differences of hyaluronan expression in oocyte and cumulus group of K1, K2 and K3 at p< 0.05, statistically. We concluded that there was no difference of hyaluronan expression on oocyte and cumulus between vitrified oocyte of pre and post in vitro maturation which indicated that oocyte could be vitrified in the immature and mature state.]]>
<![CDATA[Ekspresi Transforming Growth Factor-beta dan Growth Differentiation Factor-9 Oosit Domba yang Divitrifikasi Sesudah dan Sebelum Maturasi In Vitro ]]>
<![CDATA[Zakiyatul Faizah]]>;
<![CDATA[Raden Haryanto Aswin]]>
<![CDATA[ Jurnal Veteriner Vol 22 No 1 (2021) ]]>
Publisher : <![CDATA[Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association ]]>
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DOI: 10.19087/jveteriner.2021.22.1.109
<![CDATA[Oocyte vitrification today became a hope to preserve fertility. Its was a major challenge because of oocyte characteristic in every phase. Immature oocytes were more sensitive to osmotic stress and the membrane wes less stable while mature oocyte have spindles that were very susceptible to temperature decrease. The study aim to compare the effect of vitrification before and after in vitro maturation to the expression TGF beta and GDF9. Oocyte of ewes divided into control groups (K0), K1 maturation prior vitrification, K2 vitrification prior maturation. Vitrification begins with washing oocytes in PBS supplemented of 20%serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2).PBS + 20% serum + 0.25 M sucrose, and 3).PBS + 20% serum + 0.1 M sucrose. Immunocytochemical stain was performed to evaluate TGF ? and GDF9 expression. Remmele scale index (IRS) was used to read the result. TGF beta expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. GDF9 expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. We concluded that immature oocyte give better expression of TGF â and GDF9 than mature oocyte.]]>
<![CDATA[Effect of oocyte vitrification before and after in vitro maturation towards Bcl-2, Bax and Bcl-2/Bax ratio expression ]]>
<![CDATA[Zakiyatul Faizah]]>;
<![CDATA[R. Haryanto Aswin]]>;
<![CDATA[Hamdani Lunardhi]]>
<![CDATA[ Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/mog.V24I22016.56-60
<![CDATA[Objectives: to compare the expression of Bcl-2, Bax and Bcl-2/Bax ratio in cumulus cell and oocyte between vitrified oocyte pre and post in vitro maturation.Materials and Methods: Maturation was operated in medium TC 100 µl for 24 hours. Vitrification begins with washing oocyte in PBS basic medium supplemented of 20% serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Imunocytochemistry observed the expression of Bcl-2, bax and Bcl-2/bax ratio.Results: Bcl-2 expression on oocyte in control group differed significantly with treatment group, Bcl-2 expression on cumulus in control group differed significantly with treatment 1 group. Bax expression on oocyte in control group differed significantly with treatment group. Bax expression on cumulus in control group differed significantly with treatment group. Bcl-2/Bax expression ratio on oocyte and cumulus did not differ significantly in all groupConclusion: No difference Bcl-2/Bax expression ratio on oocyte and cumulus between vitrified oocyte pre and post in vitro maturation.]]>
<![CDATA[Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF) ]]>
<![CDATA[Audia Mumtaz Rifasky]]>;
<![CDATA[Puspa Wardhani]]>;
<![CDATA[Ashon Sa’adi]]>;
<![CDATA[Ninik Darsini]]>;
<![CDATA[Hamdani Lunardhi]]>;
<![CDATA[Zakiyatul Faizah]]>
<![CDATA[ Majalah Obstetri dan Ginekologi Vol. 29 No. 1 (2021): April ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/mog.V29I12021.14-17
<![CDATA[Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia. ]]>
<![CDATA[PEMBAGIAN PAKET SEMBAKO SEBAGAI BENTUK BANTUAN SOSIAL KEPADA PEKERJA JALANAN DI WILAYAH KELURAHAN CILENDEK TIMUR DALAM MASA TRANSISI PANDEMIK COVID-19 ]]>
<![CDATA[Hanifah Puspita Nurul H]]>;
<![CDATA[Anggi Senda Nikenindya]]>;
<![CDATA[Dhien Meutia Islamadina Wulandani]]>;
<![CDATA[Permata Aprilia Putri]]>;
<![CDATA[Cahya Ramadhanti Putri]]>;
<![CDATA[Zalfa Qanita]]>;
<![CDATA[Almira Nadya Yasmine]]>;
<![CDATA[Saskia Ramadhanti]]>;
<![CDATA[Rangga Panengah B]]>;
<![CDATA[Zakiyatul Faizah]]>
<![CDATA[ Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 2 (2020): JURNAL LAYANAN MASYARAKAT ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/jlm.v4i2.2020.486-491
<![CDATA[COVID-19 is a zoonotic disease that has the ability to transmit from animals to humans and transmission occurs through droplets, therefore the government appeal on people to carry out social distancing and quarantine. The purpose of this activity is to help food needs and facilitate the people who affected by COVID - 19 to be able to apply government program is Pola Hidup Bersih dan Sehat (PHBS) independently as an effort to prevent an increase in COVID - 19 cases. The implementation method is community service, starting with channeling social assistance in the form of distribution of groceries, hand sanitizers and liquid soap to the community around the streets of the City of Cilendek Timur, Bogor City. The results of this activity are a form of community service activities that are focused on one of the fields, namely the economic field, where the activities carried out in the form of providing social assistance by distributing nine basic goods or food to people in need.abstrakCOVID-19 merupakan penyakit zoonosis yang memiliki kemampuan menularkan dari hewan kepada manusia dan penularan terjadi melalui droplets, maka dari itu pemerintah menghimbau masyarakat untuk melakukan social distancing dan karantina. Tujuan dari kegiatan ini adalah membantu mencukupi kebutuhan pangan dan memfasilitasi masyarakat yang terdampak COVID – 19 untuk dapat menerapkan Perilaku Hidup Bersih dan Sehat (PHBS) secara mandiri sebagai upaya mencegah peningkatan kasus COVID – 19. Metode pelaksanaan adalah pengabdian masyarakat, dimulai dengan menyalurkan bantuan sosial dalam bentuk pembagian sembako, hand sanitizer dan sabun cair kepada masyarakat disekitar jalan Kelurahan Cilendek Timur Kota Bogor. Hasil dari kegiatan ini merupakan bentuk dari kegiatan pengabdian masyarakat yang difokuskan pada salah satu bidang yaitu bidang ekonomi, dimana kegiatan yang dilakukan diwujudkan dalam bentuk pemberian bantuan sosial dengan cara membagikan sembilan bahan pokok atau sembako kepada orang-orang yang membutuhkan.]]>
<![CDATA[NATURAL DEAF EDUCATION FOR THE COMMUNITY ]]>
<![CDATA[Puguh Setyo Nugroho]]>;
<![CDATA[Agustinus Agustinus]]>;
<![CDATA[William William]]>;
<![CDATA[Eko Budi Siswidiyanto]]>;
<![CDATA[Nur Rohmah]]>;
<![CDATA[Zakiyatul Faizah]]>
<![CDATA[ Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 6 No. 1 (2022): JURNAL LAYANAN MASYARAKAT ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/jlm.v6i1.2022.69-76
<![CDATA[Congenital deafness will impact the quality of life of affected individuals if they do not obtain early detection and intervention. Socialization of congenital deafness is needed, thereby the community can engage in early detection and intervention efforts. Socializing about the early detection and intervention of congenital deafness. Educational activities are carried out with counseling using the lecture method, discussion, and pretest and posttest. The average pretest result was 62.03 (± 11.51) and the posttest average was 88.39 (± 13.41). Based on the comparison between the pretest and posttest results, we found a significant difference (p < 0.0001) statistically. Public knowledge of congenital deafness needs to be improved in order to increase community participation in terms of early detection and intervention.]]>
<![CDATA[Pengaruh Seminar Online terhadap Peningkatan Pengetahuan Murid SMAN 1 Purwoasri Terkait COVID-19 pada Anak dan Remaja Selama Pandemi COVID-19 ]]>
<![CDATA[Muhammad Ircham Gustriawan]]>;
<![CDATA[Muhammad Fadhil Kamaruddin]]>;
<![CDATA[Pandya Daniswara Winantyo]]>;
<![CDATA[Zakiyatul Faizah]]>
<![CDATA[ Syntax Literate Jurnal Ilmiah Indonesia ]]>
Publisher : <![CDATA[Syntax Corporation ]]>
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DOI: 10.36418/syntax-literate.v7i1.5740
<![CDATA[Permasalahan di bidang kesehatan yang belakangan ini mendapat perhatian oleh pemerintah yaitu mengenai COVID-19. Pada tanggal 11 Maret 2020, WHO menetapkan COVID-19 sebagai pandemi sehingga mengharuskan pembelajaran dilaksanakan secara online. Dengan meningkatnya kasus COVID-19 di Indonesia, salah satu sektor yang sangat terdampak yaitu pendidikan. Tujuan utama dari penelitian ini adalah untuk meningkatkan pengetahuan dan pemahaman siswa SMA tentang COVID-19 pada anak dan remaja, serta meningkatkan kesiapan siswa-siswi dalam pembelajaran luring dan daring di era pandemi ini. Penelitian ini dilaksanakan secara daring melalui zoom dengan peserta webinar sebanyak 262 orang. Evaluasi diperoleh dari penilaian pre-test dan post- test melalui angket online yang berisi 20 pertanyaan mengenai materi COVID-19 pada anak dan remaja, evaluasi dilakukan dengan menilai peningkatan pengetahuan peserta melalui perbandingan nilai antara pre-test dan post- test, kemudian dilakukan uji statistik Wilcoxon Signed Ranks untuk mengetahui apakah terdapat perbedaan yang signifikan antara nilai pre-test dan post-test. Hasil dari pre-test dan post-test didapatkan peningkatan nilai rata-rata sebanyak 3,7% setelah dilaksanakan webinar. Didapatkan nilai rata-rata pre test responden 87% dan rerata post test sebesar 90,7%. Terdapat peningkatan pengetahuan dan pemahaman siswa terhadap pencegahan penularan virus Covid-19 setelah menghadiri seminar online.]]>
<![CDATA[Faktor Sosial Budaya yang Memengaruhi Kesehatan Ibu dan Anak (Studi Etnografi) ]]>
<![CDATA[Salsabila Salsabila]]>;
<![CDATA[Zakiyatul Faizah]]>;
<![CDATA[Budi Prasetyo]]>
<![CDATA[ Oksitosin : Jurnal Ilmiah Kebidanan Vol. 9 No. 1 (2022): OKSITOSIN : Jurnal Ilmiah Kebidanan ]]>
Publisher : <![CDATA[Prodi D III Kebidanan Fakultas Ilmu Kesehatan Universitas Ibrahimy ]]>
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DOI: 10.35316/oksitosin.v9i1.1651
<![CDATA[Maternal and child health as a key indicator of community welfare is measured by Maternal Mortality Rate (MMR) and Infant Mortality Rate (IMR). The problem of maternal and child mortality is a problem that cannot be separated from the culture or behavior of the community itself. The social and cultural system in the community is an important consideration in health services that can be utilized more optimally. The purpose of this study was to determine what cultural factors affect the health of mothers and children. This research is a rapid ethnographic assessment procedure (REAP) with a qualitative approach. Sampling technique using snowball or chain referral sampling. It is carried out sequentially from one participant to another. Is a variation of the purposive sample. The number of respondents as many as 8 people who fit the inclusion criteria. The data shown were the result of indepth interviews and FGDs (Focus Group Discussion). This study resulted in findings about: (1) Kyai become community role models in responding to life's problems ranging from political choices to choices of access to health. (2) Women do not have sufficient full power or empower themselves to choose health services, especially during pregnancy and childbirth. (3) The element of belief inherited from generation to generation also forms the knowledge system of the Cibitung community. (4) The livelihood system of farm laborers as the main source of the economy in Cibitung. Keywords: Socio-Cultural, Ethnographic, Religious Systems, Kinship Systems, Knowledge Systems, Livelihood Systems]]>
<![CDATA[HUBUNGAN USIA DAN TINGKAT STRES DENGAN KEJADIAN INFERTILITAS DI POLI OBGYN RSUD Dr. SOETOMO SURABAYA ]]>
<![CDATA[Dessy A Ningsi]]>;
<![CDATA[Zakiyatul Faizah]]>;
<![CDATA[Jimmy Yanuar Annas]]>
<![CDATA[ Indonesian Midwifery and Health Sciences Journal Vol. 3 No. 2 (2019): Indonesian Midwifery and Health Sciences Journal, April 2019 ]]>
Publisher : <![CDATA[UNIVERSITAS AIRLANGGA ]]>
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DOI: 10.20473/imhsj.v3i2.2019.179-186
<![CDATA[Abstrak Latar Belakang : Infertilitas merupakan problem yang dihadapi oleh pasangan suami istri yang telah menikah selama minimal satu tahun, melakukan hubungan seksual teratur tanpa menggunakan kontrasepsi tetapi belum berhasil memperoleh kehamilan. usia dan tingkat stres merupakan faktor yang berhubungan erat dengan tingkat kesuburan seorang wanita. Penelitian ini bertujuan untuk mengetahui hubungan antara usia dan tingkat stres dengan kejadian infertilitas. Metode : Penelitian ini adalah penelitian analitik observasional dengan desain hospital based case control di Poli Obgyn RSUD dr. Soetomo Surabaya yang dilakukan sejak bulan maret sampai bulai april 2019. Sampel yang digunakan dalam penelitian ini terdari dari 42 orang wanita infertil dan 42 orang wanita fertil yang diambil dengan metode Purposive Sampling. Data dianalisa menggunakan analisis bivariat menggunakan uji Chi Square dan uji Spearman. Hasil : Hasil penelitian menunjukkan pada kelompok kasus 76,2% responden berada pada kategori usia kurang dari 35 tahun dan pada kelompok kontrol sebanyak 81% pada kategori yang sama. Tingkat stress pada kelompok kasus dan kontrol masing - masing sebanyak 83,3% berada pada kategori normal. Hasil uji chi square antara usia dan kejadian infertilitas didapatkan p value = 0,595 (p value > 0,05). Hasil uji Spearman antara tingkat stress dengan kejadian infertilitas didapatkan p value = 0,906 (p value > 0,05). Kesimpulan: Hasil uji statistik menunjukkan tidak terdapat hubungan yang bermakna antara usia dan tingkat stress dengan kejadian infertilitas.AbstractBackground: Infertility is a problem faced by married couples who have been married for at least one year, have regular sexual intercourse without using contraception but have not succeeded in obtaining a pregnancy. Age and stress level are factors that are closely related to a woman's fertility rate. This study aims to identify the relationship between age and stress levels with the incidence of infertility. Method: This study was an observational analytic study with a hospital based case control design at Poli Obgyn RSUD dr. Soetomo Surabaya was carried out from March until April 2019. The samples used in this study were from 42 infertile women and 42 fertile women taken using the Purposive Sampling method. Data were analyzed by bivariate analysis with Chi Square test and Spearman test. Result: The results of the study showed that in the case group 76.2% of respondents were in the age group of under 35 years and the control group as much as 81% in the same category. Stress levels in the case and control groups were 83.3% in the normal category respectively. The results of the chi square test between age and the incidence of infertility obtained p value = 0.595 (p value> 0.05). The Spearman test results between stress levels and infertility events obtained p value = 0.906 (p value> 0.05). Conclusion: The results of the statistical test showed no significant relationship between age and stress level with the incidence of infertility.]]>
<![CDATA[PENGARUH PAPARAN MEDIA MASSA TERHADAP LITERASI KESEHATAN REPRODUKSI REMAJA PONDOK PESANTREN ]]>
<![CDATA[Esti Isyroofanaa]]>;
<![CDATA[Zakiyatul Faizah]]>;
<![CDATA[Martono Tri Utomo]]>
<![CDATA[ Indonesian Midwifery and Health Sciences Journal Vol. 3 No. 4 (2019): Indonesian Midwifery and Health Sciences Journal, October 2019 ]]>
Publisher : <![CDATA[UNIVERSITAS AIRLANGGA ]]>
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DOI: 10.20473/imhsj.v3i4.2019.341-348
<![CDATA[Abstrak Latar Belakang : Kesehatan merupakan salah satu di antara tiga faktor utama yang mempengaruhi Indeks Pembangunan Manusia (IPM) selain pendidikan dan pendapatan. Media dan sumber informasi kesehatan yang semakin berkembang menjadikan masyarakat lebih melek kesehatan. Pondok Pesantren Putri Hidayatul Mubtadi-aat Lirboyo Kediri menerapkan dalam penggunaan media massa mutlak tidak diperbolehkan seperti televisi, radio atau handphone. Metode : Penelitian ini merupakan penelitian analitik observasional dengan menggunakan pendekatan cross sectional. Besar sampel sebanyak 260 santri remaja umur 10 sampai 19 tahun sesuai dengan kriteria inklusi. Pengambilan data kuantitatif dilakukan dengan metode Non-Random Sampling dengan teknik consecutive sampling. Variabel independen dalam penelitian ini adalah paparan media massa, sedangkan variabel dependennya adalah tingkat health literacy kesehatan reproduksi remaja. Untuk mengetahui tingkat signifikan, data yang terkumpul akan diuji dengan uji regresi logistik sederhana dengan menggunakan derajat kepercayaan 95%. Hasil : ada pengaruh kualitas paparan media (p=0,006), kuantitas paparan media massa (p=0,002) terhadap tingkat health literacy. Kesimpulan : diperlukan lebih banyak paparan media yang berkualitas untuk meningkatkan health literacy terkait kesehatan reproduksi pada santri Pondok Pesantren Putri Hidayatul Mubtadi-aat Lirboyo Kediri.Abstract Background: Health is one of the three main factors that influence the Human Development Index (HDI) in addition to education and income. Media and health information sources that are increasingly developing make people more health literate. Islamic Boarding School, Lirboyo Kediri, is not permitted to use the mass media as television, radio or cellphones. Method: This research was an observational analytic study using a cross sectional approach. The size of samples was 260 young students aged 10 to 19 years according to the inclusion criteria. Quantitative data were retrieved Non-Randomly using consecutive sampling technique. The independent variable in this study was mass media exposure, while the dependent variable is the level of health literacy of adolescent reproductive health. To find out the effect, a simple logistic regression was applied using 95% confidence level. Results: the quality of the media that were accessed (p=0.006), the quantity of media that were accessed (p=0.002), age (p=0.000) and the training experience (p=0.000) affected health literacy individually. Conclusion: there is needs of increasing access to quality mass media in order to improve health literacy aming students of Pondok Pesantren Putri Hidayatul Mubtadi-aat Lirboyo Kediri.]]>
