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JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

JHPT TROPIKA
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Published by Universitas Lampung

ISSN : 14117525 EISSN : 24610399 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Jurnal Hama dan Penyakit Tropika (JHPT Tropika) publishes articles in plant pests, plant pathogens, plant damage caused by those pests and pathogens and or their management in tropical areas. In addition to basic and applied research papers, JHPT Tropika publishes short communication that have not been published. Before being accepted for publication, all manuscripts must be peer reviewed. The journal is published sixmonthly in March and September.

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Articles 521 Documents

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PERKEMBANGAN DAN REPRODUKSI CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) PADA PAKAN ALAMI DAN SEMIBUATAN Novalia Jelita Sari dan Djoko Prijono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 4 No. 2 (2004): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Development and reproduction of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) on natural and artificial diets. Development and reproduction of Crocidolomia pavonana on natural and artificial diets were studied in the laboratory (25.2 ± 0.9 ï‚°C; 84.6% ± 6.7% RH; ca. 12 h photophase). The natural diets tested were broccoli, cabbage, chinese cabbage, and cauliflower leaves. The artificial diets used were agar–based general lepidopteran diets mixed with red bean or broccoli leaves, and supplemented with vitamins and microbial inhibitors. Observation of insect development was done since the egg stage. After the eggs hatched, larvae were kept singly in plastic cups and fed with appropriate diets. Fifty larvae were used for each diet. Records were kept with regard to the duration of each larval instar, pupal period, and the pupal weight. The emerging adults were paired, and then the number of dead adults and that of eggs laid were recorded daily. On all the natural diets tested, the larval stage of C. pavonana passed through four instars. The egg incubation, total larval developmental, and pupal period of C. pavonana on chinese cabbage were the shortest compared to those on the other natural diets. The pupal weight, however, was the highest on broccoli, followed by that on chinese cabbage, cabbage, and cauliflower. The female fecundity was also the highest on broccoli (average 258 eggs/female) followed by that on chinese cabbage (212), cauliflower (162), and cabbage (102). Broccoli diet also yielded adults with the longest lifespan although the adult lifespan on broccoli was not significantly different from that on the other natural diets, except that of males on cabbage. C. pavonana failed to develop successfully on six kinds of artificial diets tested. The best artificial diet (broccoli–based diet with microbial inhibitors 20% of the normal amount) only yielded two males and five females with deformed wings, but none of the emerging females produced eggs.

DIAGNOSIS DAN PENGENDALIAN PENYAKIT ANTRAKNOSA PADA PAKIS DENGAN FUNGISIDA Christanti Sumardiyono; Tri Joko; Yuli Kristiawati; Yufita Dwi Chinta
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Leatherleaf fern is an important export commodity of floriculture. One of the most destructive disease on leatherleaf fern is anthracnose. The aim of this study was to identify the causal agent of antracnose-like disease on leatherleaf fern, to detect the pathogen in soil, and to control the disease with fungicides. Diagnosis was done with modified Koch Postulate. Identification of the pathogen was done by direct observation from the symptom under microscope. Isolation of the pathogen from die back leaves and soil was done on PDA. Inoculation was done on apple fruit indicator. The tested fungicides were difeconazole, benomyl, mancozeb+cymoxanil 8/64, tebuconazole, acebenzolar e-methyl+mancozeb 1/48, mancozeb, and 73.8% mancozeb + 6.2% carbendazim. In vitro test was done by poisoned food technique at 0% (checked), 0.025%, 0.05%, 0.1%, 0.2% and 0.4% concentration, while field test was done at 0.4% level concentration. Field application of pesticides was conducted by spraying twice a week for eight weeks. The result showed that the anthracnose-like disease of leatherleaf fern was anthracnose caused by Colletotrichum sp. The pathogen was found and survived in soil. In vitro test showed that 0.4% was the best concentration to inhibit mycelium growth. Based on this result, field experiment was done on 0.4% concentration of fungicides. The mixture of 73.8% mancozeb and 6.2% carbendazim was the most effective fungicide to inhibit Colletotrichum sp. in vitro. Spraying with 0.4% concentration eight times significantly reduced disease intensity in the field.

PENGOROK DAUN DAN POTENSI PARASITOIDNYA PADA BERBAGAI JENIS TANAMAN SAYURAN DI LEMBAH PALU, SULAWESI TENGAH . Shahabuddin; Flora Pasaru; . Hasriyanty
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Leafminers and potency of their parasitoids at vegetable crops in Palu Valley, Central Sulawesi. Leafminer is an exotic pest and becomes a new important pest species of onion and other vegetable crops in Central Sulawesi. The study aimed to survey the leafminer species and its infestation level at several vegetable crops as well as the potency of their parasitoids as biocontrol agents. Therefore, several crops (onions, tomato, cabbage, string bean, lemon basil, amaranth) were observed toidentify the leafminer species and their associated parasitoids. Several leaves of all crops infested by leafminers were collected for rearing the leafminers and their parasitoids in laboratory. The study recorded three species of leafminers:Liriomyza chinensis, L. sativae, and L. huidobrensis and five species of parasitoids: Hemiptarsenus varicornis, Chrysocharis pentheus, Sympiesis sp., Gronotoma micromorpha, and Opius sp. While Liriomyza chinensis was only recorded from onion crops (monophagus), other species were poliphagus. An average infestation level among leafminer species varied, i.e. 21% -51% while parasitism ranged were 13%-40 %. The most abundant parasitoid recorded from all observed crops was H. varicornis.The results suggest that parasitoids, mainly H. varicornis have a high potential to control leafminers in the vegetable crops in Palu Valley.

PENENTUAN PATOTIPE DAN KERAGAMAN GENETIK Xanthomonas oryzae pv. oryzae PADA TANAMAN PADI DI WILAYAH KARESIDENAN BANYUMAS Heru Adi Djatmiko; Budi Prakoso; Nur Prihatiningsih
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

One of the major diseases of rice paddy fields in Indonesia and the Asian countries is bacterial leaf blight or kresek caused by X. oryzae pv. oryzae (Xoo). Losses caused by the disease in Indonesia reached 70–80%, in India reached 74–81%, and Japan reached 20–50%, thus causing great losses in the economy. The objectives of that research were: 1) Characterize Xoo from Karesidenan Banyumas; 2) To study of the amount of damage and AUDPC (the area under disease progress curve) of bacterial leaf blight disease at Karesidenan Banyumas; 3) To obtain of Xoo pathotype by using the test varieties; 4) To obtaining genetic diversity of Xoo that found in Banjarnegara, Purbalingga, Banyumas, Cilacap dan Kebumen region. Research was carried out in several stages: isolation and characterization of Xoo from Barlingmascakeb region, testing of Xoo with five varieties testing, assesment of disease intensity of bacterial leaf blight and AUDPC in the field, and testing the genetic diversity of Xoo. The results showed that pathogen of bacterial blight on rice is Xoo characterized yellow colour of colonies on SPA medium, negative gram reaction, catalase positive, oxidase negative, negative growth at 0.1% TZC, negative starch hydrolisis , and resistance to 0.001% Cu(NO3)2 positive. Xoo pathotype isolats found in Banjarnegara was pathotype X, Cilacap were pathotype I and II and Purbalingga was pathotype II. Eighteen of Xoo from Karesidenan Banyumas (Banjarnegara, Purbalingga, Banyumas, Cilacap and Kebumen) of RAPD differ one from the others.

AKTIVITAS ENZIM PEROKSIDASE BAWANG MERAH YANG DIINTRODUKSI DENGAN BAKTERI ENDOFIT DAN TAHAN TERHADAP PENYAKIT HAWAR DAUN BAKTERI (XANTHOMONAS AXONOPODIS PV. ALLII) Resti, Zurai; Habazar, Trimurti; Putra, Deddi Prima; ., Nasrum
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Peroxidase enzyme activity of the introduced shallots with endophytic bacteria and resistant to bacterial leaf blightÂ (Xanthomonas axonopodis pv. allii). Bacterial leaf blight caused by Xanthomonas axonopodis pv. allii is an important disease in shallots. We have earned six isolates of endophytic bacteria, which have the ability to induce systemically resistance to shallots. One mechanism in induce resistance in plants is a change in the plant defense enzyme activity such as peroxidase. The purpose of this study was to calculate the peroxidase enzyme activity of shallots crop is being introduced with endophytic bacteria and is able to induce resistance to Xanthomonas axonopodis pv. allii. This research was conducted by introducing six isolates of endophytic bacteria on shallot bulbs and planted in greenhouse. Shallots crop that was 14 days old then inoculated with the bacterium Xanthomonas axonopodis pv. allii and incubated until symptoms appear. Peroxidase enzyme activity was calculated on the roots and leaves of shallots are 0, 1, 2, 4, 6, 8, 10, 15, and 30 days after inoculation (dai). The results showed an increase in the peroxidase enzyme activity of shallots crop is being introduced by endophytic bacteria compared to the control. Isolates ULG1E2 (Serratia marcescens PPM4) was isolate with the highest peroxidase enzyme activity both in the roots and leaves are 0,051 Ã¬m/ ml.

Pengaruh Musim terhadap Perkembangan Atelocauda digitata, Penyebab Penyakit Karat pada Acacia auricaliformis di Yogyakarta Siti Muslimah Widyastuti; . Harjono; Zjakiyah Ari Susanti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

ABSTRACTSeasonal effect on the growth of Atelocauda digitata, the cause of rust disease on Acacia auriculiformis in Yogyakarta. The research was aimed to determine the growth of Atelocauda digitata which attacks Acacia auriculiformis in Gunung Kidul, Yogyakarta and determine the process of A. digitata infection on A. auriculiformis. Quantification of the presence of pathogen and the damage as part of tree health monitoring was represented by the percentage of disease incidence and severity, that was used as a benchmark of A. digitata dynamics. Pathogenic organisms were confirmed by pathogenicity test. The process of infection was studied microscopically and macroscopically. Percentage of disease incidence and severity of all three observation plots during the rainy and the dry season were dynamic, with the highest damage discovered in Plot 5. A. digitata infecting A. auriculiformis produces three types of spores, namely teliospores, uredospores and aeciospores. The hyphae infected the leaf tissue two days after inoculation through stomata. Intracellular hyphae were found five days after inoculation.

VIRULENSI JAMUR ENTOMOPATOGEN BEAUVERIA BASSIANA (BALSAMO) VUILLEMIN (DEUTEROMYCOTINA: HYPHOMYCETES) TERHADAP KUTUDAUN (APHIS SPP.) DAN KEPIK HIJAU (NEZARA VIRIDULA) Indriyati .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 9 No. 2 (2009): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Virulence of entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina: Hyphomycetes) on aphid (Aphis spp.) and green stink bug (Nezara viridula). This study was conducted at the Laboratory of Plant Pests and Diseases Department of Plant Protection Faculty of Agriculture, University of Lampung. It was aimed to test the virulence of field and commercial inoculum of B. bassiana on aphid and green stink bug. The field inoculum of B. bassiana was originated from infected grasshoppers and isolated in Biocontrol Laboratory of PT Gunung Madu Plantations, Central Lampung, while the commercial inoculum (Natural BVR) was formulated by PT Natural Nusantara. A single exposure concentration assay (1 x 107 conidia/ml) for each inoculum was conducted by immersing the insects in fungal suspension for 10 second. The results indicated that the virulence of field and commercial inoculum of B. bassiana was significantly different on aphid . The field inoculum B. bassiana was highly virulent and caused 78.8% mortality, on the contrary the commercial B. bassiana showed low virulence and caused only 27.42% mortality of aphid. However, both inocula caused 76% and 70% mortality on green stink bug respectively. The lethal period of the field and commercial B. bassiana are 3.70 days and 3.72 days respectively on aphid, and 5.44 days and 4.58 days on green stink bug. The virulence value of both inocula are 0.271 and 0.268 on aphids, and 0.195 and 0.245 on green stink bug. The virulence of both inocula is not significantly different. This results suggests that the commercial inoculum B. bassiana at 1 x 107 conidia/ml of concentration is less effective than the field inoculum B. bassiana as control agent on aphid.

SELEKSI PSEUDOMONAD FLUORESEN SECARA LANGSUNG DI LAPANGAN UNTUK PENGENDALIAN PENYAKIT LINCAT PADA TEMBAKAU Triwidodo Arwiyanto, Fatma Yuniarsih, Toekidjo Martoredjo, dan Gembong Dalmadiyo.
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 1 (2007): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Field Screening of Pseudomonad Fluorescent for Controlling Tobacco Lincat Disease. Lincat disease is one of many factors which decreases tobacco production in Temanggung. The disease was caused by a combination between Ralstonia solanacearum and Meloidogyne incognita. The disease is difficult to control by any available control measures. The objective of this research was to find fluorescent pseudomonads which could suppress the lincat disease. The bacteria were isolated from local plants and tested in the field directly without any prior tests in the laboratory. The results indicated that dipping root system of tobacco for 20 minutes in the suspension of fluorescent pseudomonad suppressed lincat disease in the field. The results showed that there were variation in disease index between treatments. Plot treated with isolate pf22, pf23, pf30, pf42, pf51, and pf 83 showed the disease index of 16,67%; 13,34%; 20%; 20%; 16,67%; and 6,67%, respectively. However, there was an inconsistency in the capability of the isolates in the disease suppression. At the second experiment, the plants treated with pf83 isolate showed the disease index of 36,6% with the control plot of 63,3%. Laboratory tests indicated that there were 44 isolates which could inhibit pathogen growth in vitro. The fluorescent pseudomonads used in this experiments were proved not to belong to plant pathogenic bacteria.

EKSPRESI GEN PROTEIN SELUBUNG TOMATO INFECTIOUS CHLOROSIS VIRUS PADA ESCHERICHIA COLI Fitrianingrum Kurniawati; Gede Suastika; Giyanto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Expression of tomato infectious chlorosis virus coat protein gene on Escherichia coli. Tomato infectious chlorosis virus (TICV) is the causal agent of chlorotic disease of tomato. Detection of TICV can be carried out by RT-PCR and serological test. Titer of TICV in plant tissue is very low because TICV is limited to phloem. Serological detection of TICV requires antiserum which is not available in Indonesia. Producing antibody through cloning and coat protein gene (TICV CP gene) expression is a promising approach in producing antiserum. The objective of this study was to express TICV CP gene as antigen for antiserum production. TICV CP gene was amplified using RT-PCR from total RNA extracted from TICV infected leaves collected from Cipanas, Cianjur, West Java. The amplified CP gene was then sequenced and sub-cloned into pET 21b expression vector, transformed into Escherichia coli strain BL21 DE3(pLysS) and induced expression using IPTG 1 mM overnight at 37 Â°C. CP that contains 6xhistag was purified using NiNTAspin column and then confirmed by SDS-PAGE. The size of TICV CP gene was 750 bp and the gene was expressed on pET 21 b vector and SDS-PAGE showed a 29 kDa band.

PENGUJIAN TRICHODERMA SP. SEBAGAI PENGENDALI HAWAR DAUN BIBIT KAKAO YANG DISEBABKAN OLEH PHYTHOPTHORA PALMIVORA ., Sutarman
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Analysist for Trichoderma sp. as the controller of the cocoa seedlings leaf blight which caused by Phythopthora palmivora. This study aims to determine: survival and ability to live of Trichoderma sp. isolates TCN-Klp supported by predisposing wounding or without wounding the leaves as well as the ability to suppress P. palmivora inoculated on the leaves of cocoa seedlings with and without wounding the leaves before inoculation. The research conducted at the Microbiology Laboratory and Greenhouse of Agriculture Faculty, Universitas Muhammadiyah Sidoarjo on March-July 2015. There were two kinds of experiments: inoculation without wounding the leaves (1st experiment) and inoculation with wounding leaves (2nd experiment). Both of them were done by inoculation treatment: pathogens, Trichoderma, pathogens and Trichoderma simultaneously, then pathogens and incubated for 2x24 hours then inoculated. Both kinds of experiments were arranged in a complete randomize design (CRD) with 3 times of replication for 1st experiment and 4 times of replication for 2nd experiment. The data of result was analyzed by ANOVA followed by 5% test level of Duncan test. The results showed that the isolates TCN-Klp of Trichoderma sp: (i) had own survival and ability to live well in the leaf surface without injury, and (ii) was able to suppress pathogens by inoculation simultaneously, which was preceded and precede the pathogens with a gap of 24 hours on with and without wounding leaves at 10 days after inoculation.

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